Streptococcus satellite phage Javan299

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIV4|A0A4D5ZIV4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan299 OX=2558618 GN=JavanS299_0010 PE=4 SV=1
MM1 pKa = 6.37TVIYY5 pKa = 9.68IRR7 pKa = 11.84LMLEE11 pKa = 4.04SLEE14 pKa = 4.29SDD16 pKa = 3.6CILYY20 pKa = 10.69YY21 pKa = 10.42EE22 pKa = 5.57GYY24 pKa = 10.26FDD26 pKa = 4.52NLKK29 pKa = 10.89EE30 pKa = 3.92EE31 pKa = 4.53LALKK35 pKa = 10.57LDD37 pKa = 3.73VSEE40 pKa = 5.75DD41 pKa = 4.24DD42 pKa = 3.88IDD44 pKa = 3.38MTMAYY49 pKa = 7.55FTKK52 pKa = 10.51CGLIQVDD59 pKa = 3.51EE60 pKa = 4.83DD61 pKa = 4.22KK62 pKa = 11.34NAEE65 pKa = 4.03LPQAKK70 pKa = 10.02ALVQQEE76 pKa = 4.52TNQAAYY82 pKa = 8.49MRR84 pKa = 11.84EE85 pKa = 3.73YY86 pKa = 10.61RR87 pKa = 11.84KK88 pKa = 8.32QQRR91 pKa = 11.84EE92 pKa = 3.92QKK94 pKa = 10.22EE95 pKa = 4.23NLTMSSDD102 pKa = 3.49NLTLLTTCKK111 pKa = 10.34TEE113 pKa = 3.65IEE115 pKa = 4.16KK116 pKa = 10.84EE117 pKa = 3.94KK118 pKa = 10.5EE119 pKa = 3.62IEE121 pKa = 3.81IEE123 pKa = 4.03EE124 pKa = 4.39EE125 pKa = 3.78IEE127 pKa = 4.05EE128 pKa = 4.4KK129 pKa = 11.14NFFCSYY135 pKa = 10.53CC136 pKa = 3.52

Molecular weight: 16.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNX3|A0A4D5ZNX3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan299 OX=2558618 GN=JavanS299_0017 PE=4 SV=1
MM1 pKa = 7.64FDD3 pKa = 4.35GFPEE7 pKa = 4.13RR8 pKa = 11.84PRR10 pKa = 11.84YY11 pKa = 8.12MKK13 pKa = 9.81RR14 pKa = 11.84DD15 pKa = 3.77RR16 pKa = 11.84YY17 pKa = 9.2HH18 pKa = 5.94KK19 pKa = 10.09HH20 pKa = 4.58YY21 pKa = 10.97KK22 pKa = 10.24KK23 pKa = 10.13FLKK26 pKa = 10.6YY27 pKa = 9.68IEE29 pKa = 4.58KK30 pKa = 10.6GDD32 pKa = 3.91RR33 pKa = 11.84FWLNGSRR40 pKa = 11.84LL41 pKa = 3.57

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2712

41

388

135.6

15.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.863 ± 0.43

0.701 ± 0.211

5.973 ± 0.485

9.181 ± 0.954

3.761 ± 0.298

4.462 ± 0.489

1.77 ± 0.277

6.711 ± 0.368

9.292 ± 0.479

10.324 ± 0.62

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.365

4.978 ± 0.442

2.249 ± 0.313

5.494 ± 0.529

5.052 ± 0.332

5.642 ± 0.316

6.342 ± 0.408

4.425 ± 0.345

0.922 ± 0.153

4.24 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski