Pantoea phage vB_PagM_PSKM

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Dibbivirus; Pantoea virus PSKM

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513ZYN2|A0A513ZYN2_9CAUD Putative tail-completion protein OS=Pantoea phage vB_PagM_PSKM OX=2588094 GN=PSKM_gp15 PE=4 SV=1
MM1 pKa = 7.53ALINISSGIITTPNSMGATEE21 pKa = 4.13TMGYY25 pKa = 9.31LSNMVYY31 pKa = 10.3DD32 pKa = 3.54QQQRR36 pKa = 11.84KK37 pKa = 9.1DD38 pKa = 3.49VEE40 pKa = 3.92ISFDD44 pKa = 3.39AFSLVEE50 pKa = 4.38SDD52 pKa = 4.46DD53 pKa = 4.54AEE55 pKa = 4.28PTAPLTDD62 pKa = 3.4EE63 pKa = 6.29DD64 pKa = 4.27IAQHH68 pKa = 5.75RR69 pKa = 11.84QDD71 pKa = 4.66DD72 pKa = 4.26KK73 pKa = 11.95VDD75 pKa = 3.54ALSFNAGEE83 pKa = 4.41HH84 pKa = 6.94LDD86 pKa = 3.92TLSDD90 pKa = 3.67IPVAGPDD97 pKa = 3.53YY98 pKa = 11.19SDD100 pKa = 3.24TSKK103 pKa = 11.36GLQEE107 pKa = 4.02YY108 pKa = 10.02LADD111 pKa = 3.34GWIISDD117 pKa = 3.6ANQYY121 pKa = 9.75YY122 pKa = 10.72SRR124 pKa = 11.84DD125 pKa = 3.88SFDD128 pKa = 3.15LMYY131 pKa = 11.11YY132 pKa = 10.66VDD134 pKa = 4.86FKK136 pKa = 11.43GGNVLLTRR144 pKa = 11.84AKK146 pKa = 10.25KK147 pKa = 8.8ATAGAEE153 pKa = 4.3PIHH156 pKa = 7.26VPVTVKK162 pKa = 9.98WVDD165 pKa = 3.47EE166 pKa = 4.2SDD168 pKa = 4.31CICWQCGKK176 pKa = 8.77PQNSAALAANDD187 pKa = 4.28FEE189 pKa = 5.58CLNCHH194 pKa = 6.37IKK196 pKa = 10.76VSDD199 pKa = 4.59DD200 pKa = 3.35LTPCITCGKK209 pKa = 10.0VITRR213 pKa = 11.84PGGAHH218 pKa = 4.9YY219 pKa = 10.52CNRR222 pKa = 11.84KK223 pKa = 9.62DD224 pKa = 4.18ADD226 pKa = 3.87STNSS230 pKa = 3.16

Molecular weight: 25.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513ZYR1|A0A513ZYR1_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagM_PSKM OX=2588094 GN=PSKM_gp60 PE=4 SV=1
MM1 pKa = 7.47SKK3 pKa = 11.07ASIAVSNQLAATAVPVITDD22 pKa = 3.86GGRR25 pKa = 11.84QTWRR29 pKa = 11.84KK30 pKa = 8.13KK31 pKa = 10.79LVMIPMLHH39 pKa = 6.72HH40 pKa = 5.99MAQYY44 pKa = 11.08AEE46 pKa = 4.68LMDD49 pKa = 3.8KK50 pKa = 9.83FHH52 pKa = 7.52RR53 pKa = 11.84LGSVPSSHH61 pKa = 5.9YY62 pKa = 9.09RR63 pKa = 11.84HH64 pKa = 6.22RR65 pKa = 11.84LIVTGEE71 pKa = 4.02RR72 pKa = 11.84LVEE75 pKa = 4.1GAGEE79 pKa = 4.06HH80 pKa = 6.59GEE82 pKa = 4.17PKK84 pKa = 10.6VMPVYY89 pKa = 10.61HH90 pKa = 5.93FVHH93 pKa = 6.86RR94 pKa = 11.84KK95 pKa = 6.72MKK97 pKa = 10.48SRR99 pKa = 11.84AKK101 pKa = 10.28LDD103 pKa = 3.3RR104 pKa = 11.84CFPRR108 pKa = 11.84GKK110 pKa = 10.37AYY112 pKa = 10.31GRR114 pKa = 11.84IMDD117 pKa = 4.78LARR120 pKa = 11.84IRR122 pKa = 11.84AAQVKK127 pKa = 10.17AGVVV131 pKa = 3.23

Molecular weight: 14.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15278

31

852

186.3

20.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.451 ± 0.367

1.008 ± 0.141

6.428 ± 0.206

5.943 ± 0.314

3.659 ± 0.181

7.979 ± 0.356

1.99 ± 0.195

6.349 ± 0.213

5.616 ± 0.233

7.128 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.138

4.726 ± 0.21

3.613 ± 0.154

4.006 ± 0.186

5.603 ± 0.245

6.166 ± 0.2

5.727 ± 0.217

7.036 ± 0.239

1.335 ± 0.125

3.345 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski