Jodiemicrovirus-1

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0PWM8|A0A5C0PWM8_9VIRU Major capsid protein OS=Jodiemicrovirus-1 OX=2603850 PE=3 SV=1
MM1 pKa = 7.31FKK3 pKa = 10.52KK4 pKa = 9.94IRR6 pKa = 11.84YY7 pKa = 5.66VTIHH11 pKa = 6.56NYY13 pKa = 10.47KK14 pKa = 10.66EE15 pKa = 3.87MDD17 pKa = 3.33SSFEE21 pKa = 4.04YY22 pKa = 11.02VNPTKK27 pKa = 10.82VVDD30 pKa = 3.88DD31 pKa = 3.9VGYY34 pKa = 11.08LNLYY38 pKa = 8.61QYY40 pKa = 11.89AMRR43 pKa = 11.84ALKK46 pKa = 10.57GYY48 pKa = 9.48IVPISSLKK56 pKa = 9.37YY57 pKa = 10.48DD58 pKa = 3.83EE59 pKa = 5.48DD60 pKa = 4.47VSVDD64 pKa = 4.59DD65 pKa = 3.66IHH67 pKa = 8.52KK68 pKa = 10.56SASQSEE74 pKa = 4.4NQKK77 pKa = 10.5SEE79 pKa = 4.27AKK81 pKa = 10.56DD82 pKa = 3.66EE83 pKa = 4.46LNQADD88 pKa = 5.27DD89 pKa = 3.87EE90 pKa = 4.83LEE92 pKa = 4.28LYY94 pKa = 8.5EE95 pKa = 4.55AAMDD99 pKa = 4.23AGSIHH104 pKa = 7.06HH105 pKa = 7.44DD106 pKa = 2.95IDD108 pKa = 3.66PTVEE112 pKa = 4.09NEE114 pKa = 4.18KK115 pKa = 9.92STDD118 pKa = 3.73LPDD121 pKa = 3.92KK122 pKa = 10.97SEE124 pKa = 4.24PASSEE129 pKa = 3.94ATKK132 pKa = 10.73DD133 pKa = 3.21

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0PZD8|A0A5C0PZD8_9VIRU Minor capsid protein OS=Jodiemicrovirus-1 OX=2603850 PE=4 SV=1
MM1 pKa = 7.73LGPLSRR7 pKa = 11.84FLISAAPAVVKK18 pKa = 10.47LFTRR22 pKa = 11.84SKK24 pKa = 9.18PRR26 pKa = 11.84PPGRR30 pKa = 11.84VNVPPARR37 pKa = 11.84PIRR40 pKa = 11.84SIDD43 pKa = 3.53DD44 pKa = 4.15FYY46 pKa = 12.15SKK48 pKa = 11.18LNASKK53 pKa = 10.81SPSWFNRR60 pKa = 11.84LLKK63 pKa = 10.77LGAAGGAAGALGSLSPSSTGDD84 pKa = 3.41LPSGVYY90 pKa = 10.51SNPSRR95 pKa = 11.84FGYY98 pKa = 9.69FDD100 pKa = 3.59KK101 pKa = 11.26VNKK104 pKa = 10.26LSSQYY109 pKa = 9.19QNRR112 pKa = 11.84FSGFANNLDD121 pKa = 4.29TNASTPSVSGSKK133 pKa = 10.21LLSGIPGNLLSLAGGIGTGLFSRR156 pKa = 11.84YY157 pKa = 8.98LDD159 pKa = 3.24RR160 pKa = 11.84KK161 pKa = 10.44SINQSNAIQQAAEE174 pKa = 3.61DD175 pKa = 4.15RR176 pKa = 11.84AWKK179 pKa = 10.58RR180 pKa = 11.84EE181 pKa = 3.75TEE183 pKa = 4.32YY184 pKa = 11.38NHH186 pKa = 7.12PAPQMARR193 pKa = 11.84FRR195 pKa = 11.84EE196 pKa = 4.37AGLNPALMYY205 pKa = 10.7SSSLSSASGTTHH217 pKa = 7.13APLTSRR223 pKa = 11.84SSYY226 pKa = 8.45YY227 pKa = 9.29QDD229 pKa = 5.17RR230 pKa = 11.84IASYY234 pKa = 10.81LSLANMHH241 pKa = 6.36AQNKK245 pKa = 9.61LLDD248 pKa = 3.92AQARR252 pKa = 11.84SVNADD257 pKa = 3.07VFGKK261 pKa = 10.52EE262 pKa = 3.85KK263 pKa = 10.41INRR266 pKa = 11.84VLGDD270 pKa = 3.66YY271 pKa = 10.72GFSTDD276 pKa = 3.82PTWLRR281 pKa = 11.84SIFRR285 pKa = 11.84FIDD288 pKa = 3.22YY289 pKa = 10.65RR290 pKa = 11.84NNN292 pKa = 3.15

Molecular weight: 31.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1343

49

552

268.6

30.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.85 ± 1.074

1.191 ± 0.637

6.031 ± 0.828

4.393 ± 0.902

5.733 ± 0.87

5.659 ± 1.077

2.681 ± 0.589

4.914 ± 0.373

6.329 ± 1.661

8.786 ± 0.724

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.936 ± 0.254

4.84 ± 0.607

4.84 ± 0.905

2.978 ± 0.076

6.478 ± 0.678

10.276 ± 1.081

4.989 ± 0.701

5.436 ± 0.708

1.117 ± 0.214

4.542 ± 0.619
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski