Aureococcus anophagefferens (Harmful bloom alga)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Pelagophyceae; Pelagomonadales; Aureococcus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0YB55|F0YB55_AURAN AAA_12 domain-containing protein (Fragment) OS=Aureococcus anophagefferens OX=44056 GN=AURANDRAFT_17677 PE=4 SV=1
MM1 pKa = 7.42GLHH4 pKa = 6.51RR5 pKa = 11.84ALAALLVLALGAAASEE21 pKa = 4.82DD22 pKa = 3.73FSQVAKK28 pKa = 10.71LVASDD33 pKa = 4.15GAAGDD38 pKa = 3.67EE39 pKa = 4.76FGWSVASSGDD49 pKa = 3.58FVVVGAYY56 pKa = 10.56LDD58 pKa = 4.91DD59 pKa = 5.06DD60 pKa = 5.11AGTSSGSAYY69 pKa = 10.43VFRR72 pKa = 11.84TVDD75 pKa = 3.21GVTEE79 pKa = 4.12QIAKK83 pKa = 10.12LVSSDD88 pKa = 4.77AAAQDD93 pKa = 3.4QFGASVAIHH102 pKa = 6.49NDD104 pKa = 3.11IIVVGANHH112 pKa = 7.46DD113 pKa = 3.89DD114 pKa = 3.67DD115 pKa = 6.59AGYY118 pKa = 10.97DD119 pKa = 3.44SGSAYY124 pKa = 9.7IFKK127 pKa = 10.19TSDD130 pKa = 3.59GGATWPQVAKK140 pKa = 10.68LVASDD145 pKa = 4.15GAAGDD150 pKa = 3.68EE151 pKa = 4.68FGWSIASSGDD161 pKa = 3.48FVVVGANLDD170 pKa = 3.85DD171 pKa = 4.96DD172 pKa = 5.63AGTSSGSAYY181 pKa = 10.43VFRR184 pKa = 11.84TVDD187 pKa = 3.21GVTEE191 pKa = 4.12QIAKK195 pKa = 10.12LVSSDD200 pKa = 4.77AAAQDD205 pKa = 3.45QFGVSVAIHH214 pKa = 6.37NDD216 pKa = 3.16IIVVGAAWNDD226 pKa = 3.7DD227 pKa = 3.53AGSNSGSAYY236 pKa = 9.75IFKK239 pKa = 10.19TSDD242 pKa = 3.59GGATWPQVAKK252 pKa = 10.65LVADD256 pKa = 3.96DD257 pKa = 4.68AEE259 pKa = 5.53AYY261 pKa = 10.36DD262 pKa = 3.79LFGWSVAVEE271 pKa = 4.18GNLVVVGALQDD282 pKa = 3.6DD283 pKa = 4.55TNAGSAYY290 pKa = 10.47AFRR293 pKa = 11.84TTDD296 pKa = 4.46GGATWTQAAKK306 pKa = 10.23LAPGSAAYY314 pKa = 10.19HH315 pKa = 6.56FGTSLSISGNTLAVGAYY332 pKa = 7.14EE333 pKa = 4.4ANSFAGGAYY342 pKa = 10.5LFSTTDD348 pKa = 3.41NGASWTQDD356 pKa = 2.59AYY358 pKa = 11.42LQASDD363 pKa = 4.09YY364 pKa = 11.49ASADD368 pKa = 3.38RR369 pKa = 11.84FYY371 pKa = 11.2EE372 pKa = 4.0ALVLSGNRR380 pKa = 11.84LVVGATYY387 pKa = 10.99DD388 pKa = 3.27NDD390 pKa = 3.43MGGNSGSVYY399 pKa = 10.09IFEE402 pKa = 5.1APQPAPSPP410 pKa = 3.81

Molecular weight: 41.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0YT44|F0YT44_AURAN Expressed protein OS=Aureococcus anophagefferens OX=44056 GN=AURANDRAFT_69566 PE=4 SV=1
RR1 pKa = 6.84PTSSSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84SSARR13 pKa = 11.84APTPARR19 pKa = 11.84GRR21 pKa = 11.84RR22 pKa = 11.84TTTPRR27 pKa = 11.84TTTPRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84PTRR38 pKa = 11.84PRR40 pKa = 11.84SRR42 pKa = 11.84SGPTASRR49 pKa = 11.84TTRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84PTATSPAAPRR64 pKa = 11.84TRR66 pKa = 11.84TGSTSATRR74 pKa = 11.84RR75 pKa = 11.84AWRR78 pKa = 11.84RR79 pKa = 11.84STSPRR84 pKa = 11.84PSRR87 pKa = 11.84PRR89 pKa = 11.84SPRR92 pKa = 11.84TRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84GAAGSRR106 pKa = 11.84SRR108 pKa = 11.84SPRR111 pKa = 11.84PSAAPRR117 pKa = 11.84RR118 pKa = 11.84PANRR122 pKa = 11.84GRR124 pKa = 11.84GATTNRR130 pKa = 11.84GRR132 pKa = 11.84GATTTRR138 pKa = 11.84PSASPCKK145 pKa = 9.9CHH147 pKa = 7.25LVV149 pKa = 3.46

Molecular weight: 16.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11501

0

11501

6025983

29

15048

524.0

56.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.294 ± 0.056

1.833 ± 0.012

6.889 ± 0.024

5.616 ± 0.023

3.333 ± 0.014

8.227 ± 0.03

2.094 ± 0.011

2.571 ± 0.018

3.71 ± 0.024

9.232 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.592 ± 0.011

2.148 ± 0.013

5.84 ± 0.023

2.295 ± 0.013

8.156 ± 0.048

5.725 ± 0.025

4.551 ± 0.021

7.289 ± 0.022

1.342 ± 0.008

2.263 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski