Black grass cryptic virus 2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6DU17|A0A0D6DU17_9VIRU RNA dependent RNA polymerase OS=Black grass cryptic virus 2 OX=1587517 GN=RdRp PE=4 SV=1
MM1 pKa = 6.87STSEE5 pKa = 4.01GNLRR9 pKa = 11.84NDD11 pKa = 3.87PNRR14 pKa = 11.84IPEE17 pKa = 5.4PIPMDD22 pKa = 3.68IDD24 pKa = 3.7QLGHH28 pKa = 6.01SATDD32 pKa = 3.5VFDD35 pKa = 3.86PTHH38 pKa = 6.92FDD40 pKa = 3.59PEE42 pKa = 4.7GVKK45 pKa = 10.39PLVQDD50 pKa = 3.6PVAPKK55 pKa = 10.38KK56 pKa = 10.23RR57 pKa = 11.84GKK59 pKa = 6.55THH61 pKa = 6.59PRR63 pKa = 11.84SLDD66 pKa = 3.29KK67 pKa = 10.98QVVEE71 pKa = 4.45NSTPVNDD78 pKa = 3.92LRR80 pKa = 11.84RR81 pKa = 11.84VSASLAPSEE90 pKa = 5.41SRR92 pKa = 11.84FPDD95 pKa = 3.34PNMFIPNFVILFHH108 pKa = 7.03NLATMNNKK116 pKa = 8.62VCTMRR121 pKa = 11.84PFGEE125 pKa = 4.31SGTVNNWIPQVSNIVFAVLAIVQVLRR151 pKa = 11.84AQQLAGLLTPSQNRR165 pKa = 11.84FLKK168 pKa = 10.77SFIGKK173 pKa = 8.83HH174 pKa = 4.84PLEE177 pKa = 4.63TIIIPGPLLPFFRR190 pKa = 11.84SISSANPGFGNFGDD204 pKa = 4.12VTPVIPPFTQNEE216 pKa = 3.85EE217 pKa = 3.64RR218 pKa = 11.84LYY220 pKa = 10.78NIDD223 pKa = 4.27YY224 pKa = 10.35IPPSKK229 pKa = 10.53EE230 pKa = 3.64HH231 pKa = 5.64VFQGITMMLPNIPLMMDD248 pKa = 3.08QLVALVKK255 pKa = 10.55HH256 pKa = 5.82VLTLEE261 pKa = 3.74NNIPKK266 pKa = 10.09KK267 pKa = 10.82YY268 pKa = 9.51STFTQLQTIFGMNKK282 pKa = 9.56PEE284 pKa = 4.01EE285 pKa = 3.95QAPRR289 pKa = 11.84HH290 pKa = 5.19SAFVRR295 pKa = 11.84LQKK298 pKa = 10.91SIGFWQRR305 pKa = 11.84PTASDD310 pKa = 3.51GLIQRR315 pKa = 11.84FANYY319 pKa = 9.51IDD321 pKa = 4.4DD322 pKa = 4.44ACPDD326 pKa = 3.98FPAPPKK332 pKa = 10.45YY333 pKa = 10.59VSTHH337 pKa = 6.48AGTPADD343 pKa = 3.98PTQDD347 pKa = 3.4PPIPEE352 pKa = 4.51VPATWTALDD361 pKa = 4.39DD362 pKa = 4.27FAQFMCMEE370 pKa = 4.3EE371 pKa = 4.03TSVWFSKK378 pKa = 10.37FLEE381 pKa = 4.68GMHH384 pKa = 6.17VFNKK388 pKa = 8.25QWKK391 pKa = 8.94DD392 pKa = 3.36NEE394 pKa = 4.26RR395 pKa = 11.84LSNIHH400 pKa = 5.63PTKK403 pKa = 10.47SAAGLITFYY412 pKa = 10.74DD413 pKa = 3.68AEE415 pKa = 4.35YY416 pKa = 9.28TAIPTEE422 pKa = 4.04SQFRR426 pKa = 11.84DD427 pKa = 3.08RR428 pKa = 11.84SFNNATHH435 pKa = 5.74QLVMNTRR442 pKa = 11.84ATTACPHH449 pKa = 7.31IIPDD453 pKa = 4.11DD454 pKa = 5.11AIDD457 pKa = 4.08AQSSQLNAACARR469 pKa = 11.84VFNNAMTIGAPDD481 pKa = 3.49VTRR484 pKa = 11.84FGYY487 pKa = 10.25YY488 pKa = 8.24WVAKK492 pKa = 7.73PTTFQTNNGNLPDD505 pKa = 3.99MFPNVISSPDD515 pKa = 3.46YY516 pKa = 10.67FADD519 pKa = 3.41AA520 pKa = 4.46

Molecular weight: 58.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6DU17|A0A0D6DU17_9VIRU RNA dependent RNA polymerase OS=Black grass cryptic virus 2 OX=1587517 GN=RdRp PE=4 SV=1
MM1 pKa = 7.41TIEE4 pKa = 4.0AQYY7 pKa = 11.71EE8 pKa = 4.16DD9 pKa = 4.6MLKK12 pKa = 9.58MNQPPVHH19 pKa = 6.48IVQDD23 pKa = 4.02EE24 pKa = 4.39NFTTAFNLTMKK35 pKa = 10.72AFAPTEE41 pKa = 3.89KK42 pKa = 10.35VRR44 pKa = 11.84IVHH47 pKa = 5.33YY48 pKa = 10.71VRR50 pKa = 11.84TKK52 pKa = 10.01HH53 pKa = 5.75YY54 pKa = 7.67PWPLSSSVEE63 pKa = 4.25RR64 pKa = 11.84PFSTDD69 pKa = 2.83PKK71 pKa = 8.5VIKK74 pKa = 10.01YY75 pKa = 8.87VKK77 pKa = 9.48QKK79 pKa = 10.45CINGEE84 pKa = 3.89IEE86 pKa = 3.91NEE88 pKa = 3.58RR89 pKa = 11.84MSFHH93 pKa = 5.98NTFDD97 pKa = 3.54YY98 pKa = 11.33VYY100 pKa = 10.14DD101 pKa = 3.62SSRR104 pKa = 11.84LVIHH108 pKa = 6.72NIKK111 pKa = 10.28NGKK114 pKa = 9.2CFTGDD119 pKa = 3.14LTKK122 pKa = 11.17DD123 pKa = 3.06HH124 pKa = 7.58LYY126 pKa = 10.29PIIAHH131 pKa = 6.13FRR133 pKa = 11.84PGLGKK138 pKa = 9.76PGSSAIKK145 pKa = 10.15NRR147 pKa = 11.84LVWGVSKK154 pKa = 10.73IFLIAEE160 pKa = 4.28CMFMYY165 pKa = 10.61PLFDD169 pKa = 3.52NYY171 pKa = 10.54LRR173 pKa = 11.84NGKK176 pKa = 7.62TPLLWKK182 pKa = 10.81YY183 pKa = 7.38EE184 pKa = 4.1TALGGWTKK192 pKa = 10.66LYY194 pKa = 11.24NEE196 pKa = 4.78IQPQIANTNATIITADD212 pKa = 2.76WSEE215 pKa = 3.87FDD217 pKa = 3.16RR218 pKa = 11.84RR219 pKa = 11.84VPFEE223 pKa = 4.77LIEE226 pKa = 4.04MVINASLEE234 pKa = 4.22YY235 pKa = 10.64YY236 pKa = 10.69DD237 pKa = 4.97LGHH240 pKa = 6.41YY241 pKa = 10.22APDD244 pKa = 3.63YY245 pKa = 10.7LYY247 pKa = 9.25HH248 pKa = 6.44TEE250 pKa = 4.23SNCKK254 pKa = 10.18YY255 pKa = 9.12EE256 pKa = 4.16IPRR259 pKa = 11.84ILDD262 pKa = 3.37LFKK265 pKa = 8.74WTIYY269 pKa = 9.55ATLSSPLLMPDD280 pKa = 2.51GRR282 pKa = 11.84VWMRR286 pKa = 11.84TRR288 pKa = 11.84NGVPSGMFRR297 pKa = 11.84TQWLDD302 pKa = 3.48SIINGIMITTILLDD316 pKa = 3.81AGFKK320 pKa = 8.97VTEE323 pKa = 4.04KK324 pKa = 10.88FILKK328 pKa = 10.26VLGDD332 pKa = 4.13DD333 pKa = 3.62SLTVLFHH340 pKa = 6.79FVPQAKK346 pKa = 10.36HH347 pKa = 6.1EE348 pKa = 4.17DD349 pKa = 3.66LKK351 pKa = 11.68SFLAEE356 pKa = 3.79KK357 pKa = 10.69ALLRR361 pKa = 11.84FNAKK365 pKa = 10.23LSLEE369 pKa = 4.01KK370 pKa = 10.54TEE372 pKa = 4.83ISNSLYY378 pKa = 10.32GVEE381 pKa = 3.85LLGYY385 pKa = 9.86RR386 pKa = 11.84NRR388 pKa = 11.84NGAAYY393 pKa = 9.64RR394 pKa = 11.84DD395 pKa = 3.92HH396 pKa = 7.53RR397 pKa = 11.84KK398 pKa = 9.55LLAQLLFPEE407 pKa = 5.08SEE409 pKa = 4.22APTYY413 pKa = 10.72ASLMGRR419 pKa = 11.84CVGIAYY425 pKa = 10.17ADD427 pKa = 3.73LGRR430 pKa = 11.84SKK432 pKa = 11.06LLYY435 pKa = 9.67RR436 pKa = 11.84VCKK439 pKa = 10.4KK440 pKa = 10.19IFEE443 pKa = 4.44RR444 pKa = 11.84LSAAGYY450 pKa = 10.2RR451 pKa = 11.84PDD453 pKa = 2.83SRR455 pKa = 11.84QIHH458 pKa = 6.62RR459 pKa = 11.84YY460 pKa = 8.97LSGSKK465 pKa = 9.96FVEE468 pKa = 3.91TRR470 pKa = 11.84MEE472 pKa = 3.6IQINKK477 pKa = 9.08FPSTLEE483 pKa = 3.54IQKK486 pKa = 9.81WSRR489 pKa = 11.84TPYY492 pKa = 10.77NRR494 pKa = 11.84TKK496 pKa = 10.88SDD498 pKa = 3.54NDD500 pKa = 4.01HH501 pKa = 6.31YY502 pKa = 10.56WPPEE506 pKa = 3.66IFWPHH511 pKa = 4.44FTGPP515 pKa = 3.39

Molecular weight: 59.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1035

515

520

517.5

58.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.473 ± 0.679

1.063 ± 0.067

5.314 ± 0.428

4.831 ± 0.652

5.99 ± 0.49

4.638 ± 0.269

2.705 ± 0.136

6.57 ± 0.275

5.314 ± 0.971

8.213 ± 1.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.118

5.894 ± 0.681

7.923 ± 1.502

3.575 ± 0.816

5.024 ± 0.525

6.184 ± 0.02

6.667 ± 0.297

5.411 ± 0.492

1.643 ± 0.323

3.671 ± 1.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski