Amino acid dipepetide frequency for Agromyces sp. MF30-A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.146AlaAla: 22.146 ± 0.26
0.617AlaCys: 0.617 ± 0.028
8.181AlaAsp: 8.181 ± 0.105
9.382AlaGlu: 9.382 ± 0.111
4.117AlaPhe: 4.117 ± 0.062
12.886AlaGly: 12.886 ± 0.146
2.437AlaHis: 2.437 ± 0.057
6.335AlaIle: 6.335 ± 0.092
2.864AlaLys: 2.864 ± 0.061
13.98AlaLeu: 13.98 ± 0.141
2.67AlaMet: 2.67 ± 0.054
2.381AlaAsn: 2.381 ± 0.051
6.943AlaPro: 6.943 ± 0.113
3.35AlaGln: 3.35 ± 0.065
9.628AlaArg: 9.628 ± 0.129
8.451AlaSer: 8.451 ± 0.115
7.213AlaThr: 7.213 ± 0.096
11.792AlaVal: 11.792 ± 0.141
1.874AlaTrp: 1.874 ± 0.048
2.321AlaTyr: 2.321 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.592CysAla: 0.592 ± 0.024
0.037CysCys: 0.037 ± 0.006
0.284CysAsp: 0.284 ± 0.016
0.241CysGlu: 0.241 ± 0.016
0.163CysPhe: 0.163 ± 0.013
0.53CysGly: 0.53 ± 0.026
0.118CysHis: 0.118 ± 0.011
0.198CysIle: 0.198 ± 0.015
0.055CysLys: 0.055 ± 0.007
0.427CysLeu: 0.427 ± 0.022
0.082CysMet: 0.082 ± 0.009
0.095CysAsn: 0.095 ± 0.011
0.287CysPro: 0.287 ± 0.018
0.096CysGln: 0.096 ± 0.011
0.312CysArg: 0.312 ± 0.021
0.31CysSer: 0.31 ± 0.018
0.317CysThr: 0.317 ± 0.019
0.374CysVal: 0.374 ± 0.021
0.069CysTrp: 0.069 ± 0.011
0.089CysTyr: 0.089 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.284AspAla: 9.284 ± 0.119
0.249AspCys: 0.249 ± 0.018
4.416AspAsp: 4.416 ± 0.081
4.795AspGlu: 4.795 ± 0.081
1.774AspPhe: 1.774 ± 0.048
6.018AspGly: 6.018 ± 0.097
1.18AspHis: 1.18 ± 0.036
2.163AspIle: 2.163 ± 0.047
0.846AspLys: 0.846 ± 0.034
5.537AspLeu: 5.537 ± 0.084
0.676AspMet: 0.676 ± 0.026
0.825AspAsn: 0.825 ± 0.033
4.026AspPro: 4.026 ± 0.071
1.422AspGln: 1.422 ± 0.049
4.763AspArg: 4.763 ± 0.097
2.567AspSer: 2.567 ± 0.057
2.711AspThr: 2.711 ± 0.055
5.209AspVal: 5.209 ± 0.075
0.939AspTrp: 0.939 ± 0.035
1.253AspTyr: 1.253 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
8.042GluAla: 8.042 ± 0.105
0.262GluCys: 0.262 ± 0.018
2.078GluAsp: 2.078 ± 0.054
2.967GluGlu: 2.967 ± 0.067
1.976GluPhe: 1.976 ± 0.047
3.976GluGly: 3.976 ± 0.073
1.681GluHis: 1.681 ± 0.04
2.841GluIle: 2.841 ± 0.067
1.183GluLys: 1.183 ± 0.045
8.041GluLeu: 8.041 ± 0.11
0.97GluMet: 0.97 ± 0.039
1.121GluAsn: 1.121 ± 0.037
3.419GluPro: 3.419 ± 0.076
2.332GluGln: 2.332 ± 0.056
6.063GluArg: 6.063 ± 0.103
3.155GluSer: 3.155 ± 0.062
3.206GluThr: 3.206 ± 0.064
5.043GluVal: 5.043 ± 0.081
0.967GluTrp: 0.967 ± 0.032
1.243GluTyr: 1.243 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.492PheAla: 4.492 ± 0.085
0.143PheCys: 0.143 ± 0.011
2.537PheAsp: 2.537 ± 0.053
2.045PheGlu: 2.045 ± 0.053
1.013PhePhe: 1.013 ± 0.039
3.592PheGly: 3.592 ± 0.072
0.551PheHis: 0.551 ± 0.025
1.313PheIle: 1.313 ± 0.04
0.439PheLys: 0.439 ± 0.025
2.767PheLeu: 2.767 ± 0.059
0.475PheMet: 0.475 ± 0.022
0.666PheAsn: 0.666 ± 0.028
1.359PhePro: 1.359 ± 0.04
0.748PheGln: 0.748 ± 0.028
1.866PheArg: 1.866 ± 0.046
1.696PheSer: 1.696 ± 0.038
2.142PheThr: 2.142 ± 0.054
2.904PheVal: 2.904 ± 0.057
0.478PheTrp: 0.478 ± 0.029
0.635PheTyr: 0.635 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
11.658GlyAla: 11.658 ± 0.158
0.55GlyCys: 0.55 ± 0.026
5.243GlyAsp: 5.243 ± 0.083
5.463GlyGlu: 5.463 ± 0.075
3.387GlyPhe: 3.387 ± 0.07
8.078GlyGly: 8.078 ± 0.128
1.837GlyHis: 1.837 ± 0.052
4.719GlyIle: 4.719 ± 0.078
1.995GlyLys: 1.995 ± 0.054
9.08GlyLeu: 9.08 ± 0.116
1.829GlyMet: 1.829 ± 0.045
1.715GlyAsn: 1.715 ± 0.049
3.742GlyPro: 3.742 ± 0.067
2.354GlyGln: 2.354 ± 0.051
6.599GlyArg: 6.599 ± 0.098
5.412GlySer: 5.412 ± 0.088
5.402GlyThr: 5.402 ± 0.101
8.184GlyVal: 8.184 ± 0.105
1.652GlyTrp: 1.652 ± 0.048
2.262GlyTyr: 2.262 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.434HisAla: 2.434 ± 0.056
0.1HisCys: 0.1 ± 0.011
1.41HisAsp: 1.41 ± 0.043
1.29HisGlu: 1.29 ± 0.036
0.557HisPhe: 0.557 ± 0.027
2.117HisGly: 2.117 ± 0.051
0.514HisHis: 0.514 ± 0.023
0.637HisIle: 0.637 ± 0.028
0.251HisLys: 0.251 ± 0.017
1.953HisLeu: 1.953 ± 0.053
0.288HisMet: 0.288 ± 0.018
0.312HisAsn: 0.312 ± 0.015
1.534HisPro: 1.534 ± 0.043
0.436HisGln: 0.436 ± 0.025
1.596HisArg: 1.596 ± 0.043
0.968HisSer: 0.968 ± 0.031
0.869HisThr: 0.869 ± 0.03
1.708HisVal: 1.708 ± 0.05
0.29HisTrp: 0.29 ± 0.018
0.423HisTyr: 0.423 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.264IleAla: 7.264 ± 0.086
0.222IleCys: 0.222 ± 0.015
3.626IleAsp: 3.626 ± 0.076
3.245IleGlu: 3.245 ± 0.066
1.147IlePhe: 1.147 ± 0.048
4.97IleGly: 4.97 ± 0.087
0.608IleHis: 0.608 ± 0.027
1.84IleIle: 1.84 ± 0.049
0.712IleLys: 0.712 ± 0.031
3.297IleLeu: 3.297 ± 0.068
0.596IleMet: 0.596 ± 0.028
0.801IleAsn: 0.801 ± 0.034
2.248IlePro: 2.248 ± 0.051
0.85IleGln: 0.85 ± 0.032
2.767IleArg: 2.767 ± 0.054
2.246IleSer: 2.246 ± 0.055
2.761IleThr: 2.761 ± 0.05
5.01IleVal: 5.01 ± 0.077
0.528IleTrp: 0.528 ± 0.023
0.718IleTyr: 0.718 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.235LysAla: 2.235 ± 0.056
0.07LysCys: 0.07 ± 0.008
0.826LysAsp: 0.826 ± 0.035
0.899LysGlu: 0.899 ± 0.038
0.491LysPhe: 0.491 ± 0.024
1.517LysGly: 1.517 ± 0.05
0.453LysHis: 0.453 ± 0.023
0.863LysIle: 0.863 ± 0.035
0.652LysLys: 0.652 ± 0.034
1.963LysLeu: 1.963 ± 0.053
0.339LysMet: 0.339 ± 0.019
0.483LysAsn: 0.483 ± 0.026
1.17LysPro: 1.17 ± 0.04
0.745LysGln: 0.745 ± 0.031
1.643LysArg: 1.643 ± 0.041
1.092LysSer: 1.092 ± 0.038
1.213LysThr: 1.213 ± 0.043
1.623LysVal: 1.623 ± 0.047
0.258LysTrp: 0.258 ± 0.018
0.427LysTyr: 0.427 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
15.586LeuAla: 15.586 ± 0.185
0.454LeuCys: 0.454 ± 0.021
7.039LeuAsp: 7.039 ± 0.109
5.853LeuGlu: 5.853 ± 0.097
2.861LeuPhe: 2.861 ± 0.056
9.771LeuGly: 9.771 ± 0.129
1.799LeuHis: 1.799 ± 0.05
4.425LeuIle: 4.425 ± 0.087
1.693LeuLys: 1.693 ± 0.051
9.968LeuLeu: 9.968 ± 0.128
1.508LeuMet: 1.508 ± 0.042
1.686LeuAsn: 1.686 ± 0.045
5.412LeuPro: 5.412 ± 0.083
2.284LeuGln: 2.284 ± 0.055
6.988LeuArg: 6.988 ± 0.1
5.451LeuSer: 5.451 ± 0.076
5.725LeuThr: 5.725 ± 0.077
9.75LeuVal: 9.75 ± 0.124
1.197LeuTrp: 1.197 ± 0.04
1.542LeuTyr: 1.542 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
1.941MetAla: 1.941 ± 0.046
0.084MetCys: 0.084 ± 0.009
0.677MetAsp: 0.677 ± 0.029
0.594MetGlu: 0.594 ± 0.027
0.495MetPhe: 0.495 ± 0.025
1.193MetGly: 1.193 ± 0.038
0.362MetHis: 0.362 ± 0.019
0.767MetIle: 0.767 ± 0.028
0.386MetLys: 0.386 ± 0.021
1.943MetLeu: 1.943 ± 0.047
0.288MetMet: 0.288 ± 0.02
0.481MetAsn: 0.481 ± 0.024
1.188MetPro: 1.188 ± 0.033
0.558MetGln: 0.558 ± 0.027
1.387MetArg: 1.387 ± 0.035
1.334MetSer: 1.334 ± 0.045
1.495MetThr: 1.495 ± 0.045
1.252MetVal: 1.252 ± 0.04
0.197MetTrp: 0.197 ± 0.015
0.26MetTyr: 0.26 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.413AsnAla: 2.413 ± 0.053
0.12AsnCys: 0.12 ± 0.012
1.156AsnAsp: 1.156 ± 0.039
1.058AsnGlu: 1.058 ± 0.035
0.604AsnPhe: 0.604 ± 0.031
1.91AsnGly: 1.91 ± 0.053
0.355AsnHis: 0.355 ± 0.02
0.786AsnIle: 0.786 ± 0.029
0.335AsnLys: 0.335 ± 0.022
1.793AsnLeu: 1.793 ± 0.046
0.288AsnMet: 0.288 ± 0.018
0.409AsnAsn: 0.409 ± 0.024
1.552AsnPro: 1.552 ± 0.041
0.528AsnGln: 0.528 ± 0.026
1.308AsnArg: 1.308 ± 0.04
0.947AsnSer: 0.947 ± 0.033
1.094AsnThr: 1.094 ± 0.035
1.587AsnVal: 1.587 ± 0.046
0.277AsnTrp: 0.277 ± 0.017
0.441AsnTyr: 0.441 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
7.588ProAla: 7.588 ± 0.108
0.169ProCys: 0.169 ± 0.015
3.656ProAsp: 3.656 ± 0.067
4.081ProGlu: 4.081 ± 0.079
1.772ProPhe: 1.772 ± 0.047
5.251ProGly: 5.251 ± 0.086
1.053ProHis: 1.053 ± 0.031
2.289ProIle: 2.289 ± 0.055
1.165ProLys: 1.165 ± 0.042
4.827ProLeu: 4.827 ± 0.074
0.859ProMet: 0.859 ± 0.031
1.06ProAsn: 1.06 ± 0.034
2.354ProPro: 2.354 ± 0.059
1.279ProGln: 1.279 ± 0.042
3.412ProArg: 3.412 ± 0.079
3.159ProSer: 3.159 ± 0.065
3.129ProThr: 3.129 ± 0.066
5.076ProVal: 5.076 ± 0.09
0.837ProTrp: 0.837 ± 0.029
1.011ProTyr: 1.011 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.39GlnAla: 3.39 ± 0.064
0.108GlnCys: 0.108 ± 0.01
0.911GlnAsp: 0.911 ± 0.038
1.103GlnGlu: 1.103 ± 0.038
0.881GlnPhe: 0.881 ± 0.031
1.954GlnGly: 1.954 ± 0.043
0.651GlnHis: 0.651 ± 0.03
1.246GlnIle: 1.246 ± 0.037
0.582GlnLys: 0.582 ± 0.029
3.109GlnLeu: 3.109 ± 0.061
0.426GlnMet: 0.426 ± 0.02
0.586GlnAsn: 0.586 ± 0.022
1.443GlnPro: 1.443 ± 0.047
1.083GlnGln: 1.083 ± 0.042
2.247GlnArg: 2.247 ± 0.058
1.444GlnSer: 1.444 ± 0.039
1.396GlnThr: 1.396 ± 0.039
2.287GlnVal: 2.287 ± 0.051
0.422GlnTrp: 0.422 ± 0.02
0.548GlnTyr: 0.548 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
9.487ArgAla: 9.487 ± 0.134
0.316ArgCys: 0.316 ± 0.02
4.215ArgAsp: 4.215 ± 0.08
4.604ArgGlu: 4.604 ± 0.07
2.565ArgPhe: 2.565 ± 0.053
5.56ArgGly: 5.56 ± 0.086
1.687ArgHis: 1.687 ± 0.043
3.688ArgIle: 3.688 ± 0.072
1.276ArgLys: 1.276 ± 0.037
7.821ArgLeu: 7.821 ± 0.106
1.628ArgMet: 1.628 ± 0.039
1.387ArgAsn: 1.387 ± 0.037
3.743ArgPro: 3.743 ± 0.072
1.926ArgGln: 1.926 ± 0.049
6.867ArgArg: 6.867 ± 0.107
4.303ArgSer: 4.303 ± 0.076
3.966ArgThr: 3.966 ± 0.065
6.286ArgVal: 6.286 ± 0.098
1.106ArgTrp: 1.106 ± 0.031
1.583ArgTyr: 1.583 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
7.297SerAla: 7.297 ± 0.11
0.275SerCys: 0.275 ± 0.016
3.116SerAsp: 3.116 ± 0.062
2.809SerGlu: 2.809 ± 0.066
1.881SerPhe: 1.881 ± 0.052
5.996SerGly: 5.996 ± 0.083
0.987SerHis: 0.987 ± 0.038
2.718SerIle: 2.718 ± 0.053
1.125SerLys: 1.125 ± 0.035
5.254SerLeu: 5.254 ± 0.082
1.153SerMet: 1.153 ± 0.04
1.171SerAsn: 1.171 ± 0.037
3.146SerPro: 3.146 ± 0.058
1.312SerGln: 1.312 ± 0.04
3.917SerArg: 3.917 ± 0.074
3.395SerSer: 3.395 ± 0.072
3.55SerThr: 3.55 ± 0.07
4.766SerVal: 4.766 ± 0.076
0.873SerTrp: 0.873 ± 0.033
1.2SerTyr: 1.2 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
7.483ThrAla: 7.483 ± 0.102
0.234ThrCys: 0.234 ± 0.021
3.392ThrAsp: 3.392 ± 0.061
3.121ThrGlu: 3.121 ± 0.062
1.769ThrPhe: 1.769 ± 0.046
5.85ThrGly: 5.85 ± 0.095
1.04ThrHis: 1.04 ± 0.033
2.834ThrIle: 2.834 ± 0.055
1.183ThrLys: 1.183 ± 0.039
5.609ThrLeu: 5.609 ± 0.081
0.895ThrMet: 0.895 ± 0.029
1.133ThrAsn: 1.133 ± 0.04
3.71ThrPro: 3.71 ± 0.066
1.262ThrGln: 1.262 ± 0.036
3.632ThrArg: 3.632 ± 0.063
3.107ThrSer: 3.107 ± 0.068
3.48ThrThr: 3.48 ± 0.071
5.533ThrVal: 5.533 ± 0.096
0.748ThrTrp: 0.748 ± 0.032
0.908ThrTyr: 0.908 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
12.312ValAla: 12.312 ± 0.131
0.459ValCys: 0.459 ± 0.022
5.925ValAsp: 5.925 ± 0.098
5.324ValGlu: 5.324 ± 0.089
3.108ValPhe: 3.108 ± 0.064
7.112ValGly: 7.112 ± 0.102
1.744ValHis: 1.744 ± 0.046
4.46ValIle: 4.46 ± 0.07
1.634ValLys: 1.634 ± 0.046
9.564ValLeu: 9.564 ± 0.127
1.374ValMet: 1.374 ± 0.036
1.791ValAsn: 1.791 ± 0.05
5.037ValPro: 5.037 ± 0.079
2.212ValGln: 2.212 ± 0.05
6.093ValArg: 6.093 ± 0.094
4.828ValSer: 4.828 ± 0.075
5.4ValThr: 5.4 ± 0.089
9.114ValVal: 9.114 ± 0.128
1.163ValTrp: 1.163 ± 0.039
1.523ValTyr: 1.523 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.69TrpAla: 1.69 ± 0.049
0.099TrpCys: 0.099 ± 0.01
0.657TrpAsp: 0.657 ± 0.029
0.544TrpGlu: 0.544 ± 0.023
0.583TrpPhe: 0.583 ± 0.025
0.996TrpGly: 0.996 ± 0.038
0.309TrpHis: 0.309 ± 0.02
0.73TrpIle: 0.73 ± 0.029
0.299TrpLys: 0.299 ± 0.018
1.842TrpLeu: 1.842 ± 0.059
0.297TrpMet: 0.297 ± 0.018
0.442TrpAsn: 0.442 ± 0.022
0.754TrpPro: 0.754 ± 0.03
0.541TrpGln: 0.541 ± 0.022
1.244TrpArg: 1.244 ± 0.043
0.974TrpSer: 0.974 ± 0.033
0.748TrpThr: 0.748 ± 0.031
1.159TrpVal: 1.159 ± 0.037
0.344TrpTrp: 0.344 ± 0.021
0.298TrpTyr: 0.298 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.234TyrAla: 2.234 ± 0.047
0.108TyrCys: 0.108 ± 0.012
1.311TyrAsp: 1.311 ± 0.04
1.147TyrGlu: 1.147 ± 0.039
0.72TyrPhe: 0.72 ± 0.028
1.884TyrGly: 1.884 ± 0.043
0.321TyrHis: 0.321 ± 0.021
0.604TyrIle: 0.604 ± 0.028
0.287TyrLys: 0.287 ± 0.019
2.215TyrLeu: 2.215 ± 0.048
0.238TyrMet: 0.238 ± 0.017
0.418TyrAsn: 0.418 ± 0.021
0.957TyrPro: 0.957 ± 0.031
0.5TyrGln: 0.5 ± 0.023
1.678TyrArg: 1.678 ± 0.044
1.101TyrSer: 1.101 ± 0.039
1.108TyrThr: 1.108 ± 0.038
1.521TyrVal: 1.521 ± 0.038
0.332TyrTrp: 0.332 ± 0.019
0.444TyrTyr: 0.444 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2774 proteins (909229 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski