Arthrobacter phage Idaho

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T834|A0A4D6T834_9CAUD Uncharacterized protein OS=Arthrobacter phage Idaho OX=2565509 GN=1 PE=4 SV=1
MM1 pKa = 7.84PNLTDD6 pKa = 5.65LLTLTKK12 pKa = 9.82RR13 pKa = 11.84AIMGSPSPLEE23 pKa = 4.39FGSSTSGGYY32 pKa = 7.41TWADD36 pKa = 3.02GMALSPILPPSIAGGAVSVKK56 pKa = 10.43DD57 pKa = 3.98ALRR60 pKa = 11.84CPPVARR66 pKa = 11.84GVGLYY71 pKa = 9.71AAAMLQMDD79 pKa = 4.98WEE81 pKa = 5.62AGDD84 pKa = 3.89ATKK87 pKa = 10.7AWLNKK92 pKa = 9.8GSGIVSPRR100 pKa = 11.84LRR102 pKa = 11.84NVRR105 pKa = 11.84TMTDD109 pKa = 3.0LLLANRR115 pKa = 11.84ALWITSGTGYY125 pKa = 10.73DD126 pKa = 3.35RR127 pKa = 11.84QEE129 pKa = 4.02AVHH132 pKa = 6.29LHH134 pKa = 4.91AHH136 pKa = 6.39LWSVDD141 pKa = 3.48EE142 pKa = 5.37LGTISLSGVLPDD154 pKa = 4.37ANGDD158 pKa = 3.72YY159 pKa = 11.55VMGGRR164 pKa = 11.84VVTNVANLIYY174 pKa = 10.5FPGAMPLGFLEE185 pKa = 4.24NAQDD189 pKa = 3.64SLSYY193 pKa = 10.96YY194 pKa = 10.76LDD196 pKa = 3.06ILATIKK202 pKa = 10.62SRR204 pKa = 11.84AAAPMAVMEE213 pKa = 4.5LKK215 pKa = 10.47ILDD218 pKa = 4.36SYY220 pKa = 11.5DD221 pKa = 3.44GPEE224 pKa = 4.85PGDD227 pKa = 3.7DD228 pKa = 5.72DD229 pKa = 4.85YY230 pKa = 12.07DD231 pKa = 4.08EE232 pKa = 6.68DD233 pKa = 4.66EE234 pKa = 4.81DD235 pKa = 6.04PIRR238 pKa = 11.84QTQAAYY244 pKa = 9.58VAARR248 pKa = 11.84RR249 pKa = 11.84MPDD252 pKa = 2.89GAVTVTPKK260 pKa = 10.82DD261 pKa = 2.96VDD263 pKa = 4.89LIVHH267 pKa = 5.09QVEE270 pKa = 4.92DD271 pKa = 4.54DD272 pKa = 3.32GHH274 pKa = 5.79MLIEE278 pKa = 4.05ARR280 pKa = 11.84AALRR284 pKa = 11.84VDD286 pKa = 3.86VANHH290 pKa = 6.05LNMPASMLDD299 pKa = 3.48GEE301 pKa = 4.97TGSSSDD307 pKa = 4.35YY308 pKa = 11.59ANTLQKK314 pKa = 10.69RR315 pKa = 11.84NEE317 pKa = 4.03FEE319 pKa = 4.47DD320 pKa = 4.02LSLALFTDD328 pKa = 5.68PIAARR333 pKa = 11.84LSMDD337 pKa = 3.79DD338 pKa = 3.33VTPPGEE344 pKa = 4.1LVDD347 pKa = 5.83FKK349 pKa = 10.41PLDD352 pKa = 3.7MAPVAVGNTGDD363 pKa = 4.3AVGNLQAGQLPRR375 pKa = 11.84DD376 pKa = 3.65NKK378 pKa = 10.3NRR380 pKa = 11.84EE381 pKa = 3.75ITRR384 pKa = 11.84DD385 pKa = 3.65PEE387 pKa = 3.85NALL390 pKa = 3.63

Molecular weight: 41.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6TBT1|A0A4D6TBT1_9CAUD HNH endonuclease OS=Arthrobacter phage Idaho OX=2565509 GN=21 PE=4 SV=1
MM1 pKa = 7.48GGSLLTPSAKK11 pKa = 10.01RR12 pKa = 11.84VDD14 pKa = 4.14EE15 pKa = 4.28LRR17 pKa = 11.84AAALALRR24 pKa = 11.84LLPKK28 pKa = 10.58GIRR31 pKa = 11.84NDD33 pKa = 2.95INKK36 pKa = 8.88NRR38 pKa = 11.84RR39 pKa = 11.84AVLNPMWRR47 pKa = 11.84DD48 pKa = 3.11AVNAKK53 pKa = 10.27AVTTMDD59 pKa = 3.83KK60 pKa = 11.05LVLAKK65 pKa = 10.11GARR68 pKa = 11.84VTPGNPARR76 pKa = 11.84AMAATSKK83 pKa = 10.57RR84 pKa = 11.84PLSGGLVPDD93 pKa = 4.8DD94 pKa = 4.59RR95 pKa = 11.84DD96 pKa = 3.42TAVAFEE102 pKa = 4.3FGSPEE107 pKa = 3.72RR108 pKa = 11.84QKK110 pKa = 10.33EE111 pKa = 3.83VEE113 pKa = 4.06YY114 pKa = 10.98ARR116 pKa = 11.84RR117 pKa = 11.84SPKK120 pKa = 10.32GGRR123 pKa = 11.84HH124 pKa = 3.38QVKK127 pKa = 9.68RR128 pKa = 11.84HH129 pKa = 5.18TKK131 pKa = 9.26RR132 pKa = 11.84QLPMLARR139 pKa = 11.84KK140 pKa = 9.68GRR142 pKa = 11.84VIYY145 pKa = 9.78AAWKK149 pKa = 8.06ATAPRR154 pKa = 11.84IIALDD159 pKa = 3.6TQTIARR165 pKa = 11.84LIYY168 pKa = 9.54RR169 pKa = 11.84AYY171 pKa = 9.91EE172 pKa = 4.27GKK174 pKa = 10.8SNN176 pKa = 3.67

Molecular weight: 19.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

4897

35

608

222.6

23.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.825 ± 0.675

0.49 ± 0.158

7.066 ± 0.506

5.228 ± 0.391

2.205 ± 0.207

8.822 ± 0.292

1.348 ± 0.18

3.982 ± 0.235

4.288 ± 0.514

8.863 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.225

3.206 ± 0.235

4.962 ± 0.528

3.717 ± 0.268

6.106 ± 0.528

5.473 ± 0.272

6.637 ± 0.376

7.106 ± 0.408

1.838 ± 0.202

2.287 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski