Cellulophaga phage phi19:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZW40|R9ZW40_9CAUD Uncharacterized protein OS=Cellulophaga phage phi19:1 OX=1327970 GN=Phi19:1_gp016 PE=4 SV=1
MM1 pKa = 7.74ASLAQLTAQIDD12 pKa = 3.66ILTQNVSDD20 pKa = 5.17LNDD23 pKa = 3.4TVAAILSNTIIVPNYY38 pKa = 9.37VINASDD44 pKa = 4.02PNVKK48 pKa = 10.43VGDD51 pKa = 3.79KK52 pKa = 10.6VIGYY56 pKa = 7.38GFPNINGGGNFMADD70 pKa = 3.35VVSLPISTYY79 pKa = 11.44ANISNPIEE87 pKa = 3.94WW88 pKa = 3.7

Molecular weight: 9.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9ZW07|R9ZW07_9CAUD Uncharacterized protein OS=Cellulophaga phage phi19:1 OX=1327970 GN=Phi19:1_gp089 PE=4 SV=1
MM1 pKa = 7.4NNKK4 pKa = 7.97QRR6 pKa = 11.84KK7 pKa = 8.64LEE9 pKa = 4.08YY10 pKa = 10.03IRR12 pKa = 11.84AKK14 pKa = 10.29NALRR18 pKa = 11.84LRR20 pKa = 11.84KK21 pKa = 9.3IHH23 pKa = 6.58FLRR26 pKa = 11.84FDD28 pKa = 3.38VAEE31 pKa = 4.12VMTNQQSFVLNEE43 pKa = 3.96FVNKK47 pKa = 9.59QLNRR51 pKa = 11.84YY52 pKa = 5.97TLMRR56 pKa = 11.84NKK58 pKa = 9.65IFNVNYY64 pKa = 9.48EE65 pKa = 4.08YY66 pKa = 11.61NLIDD70 pKa = 3.67FTCGYY75 pKa = 9.88LGYY78 pKa = 10.69GFEE81 pKa = 5.0KK82 pKa = 10.41GLKK85 pKa = 8.57TIRR88 pKa = 11.84IKK90 pKa = 10.84YY91 pKa = 8.43KK92 pKa = 10.45RR93 pKa = 11.84KK94 pKa = 9.69

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

17594

36

1147

155.7

17.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.104 ± 0.422

1.194 ± 0.153

6.275 ± 0.207

7.065 ± 0.328

4.547 ± 0.182

5.491 ± 0.263

1.358 ± 0.15

8.065 ± 0.235

9.151 ± 0.464

8.759 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.135

7.218 ± 0.242

2.336 ± 0.149

2.882 ± 0.195

3.473 ± 0.169

7.105 ± 0.303

6.315 ± 0.32

5.985 ± 0.21

1.159 ± 0.105

4.388 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski