Aureobasidium namibiae CBS 147.97

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Dothideales; Saccotheciaceae; Aureobasidium; Aureobasidium namibiae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074XGN1|A0A074XGN1_9PEZI TPT domain-containing protein OS=Aureobasidium namibiae CBS 147.97 OX=1043004 GN=M436DRAFT_45443 PE=4 SV=1
MM1 pKa = 6.6TTPDD5 pKa = 3.4GSPWVAVSKK14 pKa = 9.89TPTTINLHH22 pKa = 6.58ANTSAPSNTTSSDD35 pKa = 3.26QSIATNDD42 pKa = 3.1RR43 pKa = 11.84HH44 pKa = 7.59LSDD47 pKa = 3.98LRR49 pKa = 11.84SWLYY53 pKa = 10.33ATFSWGYY60 pKa = 6.08EE61 pKa = 4.01TPAASSAVAAPSTQATYY78 pKa = 10.79AAASSAAHH86 pKa = 6.12SSATGEE92 pKa = 4.28VLATPADD99 pKa = 3.56NGAEE103 pKa = 4.02YY104 pKa = 10.47LSPVTIGGQKK114 pKa = 10.33LDD116 pKa = 4.25LNFDD120 pKa = 3.93TGSSDD125 pKa = 3.07LWVFSTALTSAQIGEE140 pKa = 4.23HH141 pKa = 5.66SAYY144 pKa = 10.4DD145 pKa = 3.72PSKK148 pKa = 10.61SSSYY152 pKa = 9.97QQLDD156 pKa = 3.03GSTFSLSYY164 pKa = 11.28GDD166 pKa = 3.92GSGAAGVVGFDD177 pKa = 3.34TVDD180 pKa = 2.66IGGATVTRR188 pKa = 11.84QAIEE192 pKa = 4.0LATSVSSAFVSDD204 pKa = 3.92ADD206 pKa = 3.7SDD208 pKa = 4.37GLVGLAFSTLNSVSPRR224 pKa = 11.84PQKK227 pKa = 9.92TFFDD231 pKa = 4.42NIMSEE236 pKa = 4.26LAQPVFTAALDD247 pKa = 3.91LDD249 pKa = 4.16GSGTYY254 pKa = 10.34EE255 pKa = 4.23FGTIDD260 pKa = 3.2SSKK263 pKa = 9.87FTGDD267 pKa = 3.49LQFTPVNASSGFWQVDD283 pKa = 3.55SNRR286 pKa = 11.84YY287 pKa = 9.48SIGGKK292 pKa = 9.92DD293 pKa = 3.85CDD295 pKa = 3.7RR296 pKa = 11.84TNASPAIIDD305 pKa = 3.66TGTSLLLLDD314 pKa = 4.51PEE316 pKa = 4.84VVQAYY321 pKa = 9.74YY322 pKa = 11.4ADD324 pKa = 4.19IPSASYY330 pKa = 10.89DD331 pKa = 3.75STVGGYY337 pKa = 8.5TYY339 pKa = 10.81DD340 pKa = 3.57CSEE343 pKa = 4.62SLPEE347 pKa = 3.74FAVAVGDD354 pKa = 3.74YY355 pKa = 7.91MANIPGSGITFAPVSTKK372 pKa = 9.49TCFGGVQSNSGSDD385 pKa = 3.37LQIFGDD391 pKa = 4.09VLLKK395 pKa = 10.57HH396 pKa = 6.31HH397 pKa = 6.44FVVFNGQSEE406 pKa = 5.07VIGMAAKK413 pKa = 10.67AEE415 pKa = 4.12

Molecular weight: 43.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074X0M6|A0A074X0M6_9PEZI Uncharacterized protein OS=Aureobasidium namibiae CBS 147.97 OX=1043004 GN=M436DRAFT_36588 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNTIRR36 pKa = 11.84YY37 pKa = 5.73NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10258

0

10258

4634218

49

4952

451.8

49.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.927 ± 0.019

1.243 ± 0.01

5.824 ± 0.015

5.972 ± 0.022

3.728 ± 0.015

6.556 ± 0.023

2.427 ± 0.01

4.893 ± 0.014

4.998 ± 0.024

8.811 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.011

3.828 ± 0.012

5.756 ± 0.025

4.191 ± 0.019

5.773 ± 0.021

8.275 ± 0.038

6.153 ± 0.021

6.13 ± 0.017

1.448 ± 0.01

2.825 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski