Erythrura gouldiae polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Gammapolyomavirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1ETR3|A0A0K1ETR3_9POLY Large T antigen OS=Erythrura gouldiae polyomavirus 1 OX=2169903 PE=4 SV=1
MM1 pKa = 7.45SKK3 pKa = 11.07ADD5 pKa = 3.27IAEE8 pKa = 4.03LRR10 pKa = 11.84EE11 pKa = 4.0LLQLPKK17 pKa = 10.39NASFEE22 pKa = 4.48KK23 pKa = 10.43IKK25 pKa = 10.69AAYY28 pKa = 8.77RR29 pKa = 11.84KK30 pKa = 9.1KK31 pKa = 10.53ALMYY35 pKa = 10.6HH36 pKa = 6.72PDD38 pKa = 3.36KK39 pKa = 11.61GGDD42 pKa = 3.52EE43 pKa = 4.35EE44 pKa = 5.1KK45 pKa = 10.31MKK47 pKa = 10.69RR48 pKa = 11.84LNSLMEE54 pKa = 3.69QAKK57 pKa = 9.26TYY59 pKa = 10.91DD60 pKa = 3.72FSAEE64 pKa = 3.81NRR66 pKa = 11.84EE67 pKa = 3.63EE68 pKa = 4.46DD69 pKa = 4.22LYY71 pKa = 11.48CEE73 pKa = 4.42EE74 pKa = 4.52SLSSEE79 pKa = 4.32EE80 pKa = 4.46EE81 pKa = 4.17DD82 pKa = 3.77VSPGPSQRR90 pKa = 11.84AEE92 pKa = 3.82DD93 pKa = 3.31TGYY96 pKa = 11.49GSFSASFNASFTDD109 pKa = 3.66SVSALEE115 pKa = 4.22FQHH118 pKa = 6.95ACTKK122 pKa = 8.79ITKK125 pKa = 9.89LQDD128 pKa = 2.87KK129 pKa = 9.85LLAYY133 pKa = 9.32FAYY136 pKa = 10.78VDD138 pKa = 3.97GQRR141 pKa = 11.84KK142 pKa = 8.03QCLAQPYY149 pKa = 9.37RR150 pKa = 11.84DD151 pKa = 3.95LKK153 pKa = 10.58RR154 pKa = 11.84AYY156 pKa = 10.18QGVPWNLFNEE166 pKa = 4.7HH167 pKa = 5.37SHH169 pKa = 6.08FF170 pKa = 4.44

Molecular weight: 19.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1EUA9|A0A0K1EUA9_9POLY Capsid protein VP1 OS=Erythrura gouldiae polyomavirus 1 OX=2169903 PE=3 SV=1
MM1 pKa = 7.4AVAVWRR7 pKa = 11.84PEE9 pKa = 3.54VDD11 pKa = 4.04FLFPGASEE19 pKa = 4.8LVDD22 pKa = 3.25ALYY25 pKa = 10.79YY26 pKa = 10.27INPLEE31 pKa = 4.27WGPTIFQNIGRR42 pKa = 11.84YY43 pKa = 8.0IWDD46 pKa = 3.48YY47 pKa = 11.12VIQTGRR53 pKa = 11.84RR54 pKa = 11.84QLGEE58 pKa = 3.67ATRR61 pKa = 11.84AIVQTTSSNLYY72 pKa = 10.41DD73 pKa = 3.91LAARR77 pKa = 11.84AAEE80 pKa = 3.92RR81 pKa = 11.84ATWYY85 pKa = 8.45ITEE88 pKa = 4.18GPIRR92 pKa = 11.84SYY94 pKa = 11.33RR95 pKa = 11.84FLEE98 pKa = 4.47DD99 pKa = 3.8YY100 pKa = 11.36YY101 pKa = 10.85MEE103 pKa = 4.85LPILRR108 pKa = 11.84PDD110 pKa = 3.28ARR112 pKa = 11.84RR113 pKa = 11.84GRR115 pKa = 11.84ALAIGSDD122 pKa = 3.8AEE124 pKa = 4.28EE125 pKa = 4.48EE126 pKa = 4.1EE127 pKa = 4.59LPEE130 pKa = 4.46GLRR133 pKa = 11.84KK134 pKa = 9.72LHH136 pKa = 6.64PKK138 pKa = 7.77QTHH141 pKa = 5.77EE142 pKa = 4.07PTGATVAPSVAPGGANQRR160 pKa = 11.84HH161 pKa = 6.0CPDD164 pKa = 2.74WMLPLILGLYY174 pKa = 8.72GVVYY178 pKa = 9.34PGWKK182 pKa = 10.28AEE184 pKa = 3.97VEE186 pKa = 3.99LLEE189 pKa = 5.21KK190 pKa = 10.88EE191 pKa = 4.44EE192 pKa = 4.22SQHH195 pKa = 5.9GSKK198 pKa = 9.2TGPYY202 pKa = 8.8RR203 pKa = 11.84RR204 pKa = 11.84SRR206 pKa = 11.84SKK208 pKa = 10.55PRR210 pKa = 11.84SKK212 pKa = 9.45TPYY215 pKa = 8.45QRR217 pKa = 11.84RR218 pKa = 11.84HH219 pKa = 5.39RR220 pKa = 11.84SSRR223 pKa = 11.84SKK225 pKa = 10.43VRR227 pKa = 11.84SRR229 pKa = 3.57

Molecular weight: 26.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1878

144

634

313.0

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.253 ± 0.927

1.651 ± 0.497

4.739 ± 0.491

7.295 ± 0.533

3.248 ± 0.443

7.401 ± 0.781

2.13 ± 0.27

4.313 ± 0.542

5.325 ± 0.872

10.064 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.917 ± 0.265

3.994 ± 0.6

7.295 ± 1.142

3.834 ± 0.179

6.337 ± 0.762

6.55 ± 0.53

6.07 ± 0.359

4.846 ± 0.638

1.225 ± 0.326

3.514 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski