Tomato severe leaf curl virus-[Guatemala 96-1]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato severe leaf curl virus

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9WHE6|Q9WHE6_9GEMI Capsid protein OS=Tomato severe leaf curl virus-[Guatemala 96-1] OX=223361 GN=cp PE=3 SV=2
MM1 pKa = 7.63PRR3 pKa = 11.84NPNLFRR9 pKa = 11.84LAAKK13 pKa = 10.33NIFLTYY19 pKa = 8.23PQCDD23 pKa = 3.36IPKK26 pKa = 10.32DD27 pKa = 3.57EE28 pKa = 5.61AIEE31 pKa = 3.92MLQNLPWSVVKK42 pKa = 8.51PTYY45 pKa = 10.14IRR47 pKa = 11.84VARR50 pKa = 11.84EE51 pKa = 3.38EE52 pKa = 4.12HH53 pKa = 6.9ADD55 pKa = 3.74GFPHH59 pKa = 6.45LHH61 pKa = 6.82CLIQLSGKK69 pKa = 10.23SNIKK73 pKa = 9.85DD74 pKa = 3.18ARR76 pKa = 11.84FFDD79 pKa = 3.84LTHH82 pKa = 6.84PRR84 pKa = 11.84RR85 pKa = 11.84SANFHH90 pKa = 6.82PNAQAAKK97 pKa = 8.99DD98 pKa = 3.72TNAVQNYY105 pKa = 4.66ITKK108 pKa = 10.44EE109 pKa = 3.51GDD111 pKa = 3.42YY112 pKa = 10.99CEE114 pKa = 4.36SGQYY118 pKa = 9.59KK119 pKa = 10.27VSGGTKK125 pKa = 9.48SNKK128 pKa = 9.65DD129 pKa = 3.55DD130 pKa = 3.96VYY132 pKa = 11.43HH133 pKa = 6.35NAVNAASAGEE143 pKa = 3.88ALDD146 pKa = 4.38IIRR149 pKa = 11.84AGDD152 pKa = 3.58PKK154 pKa = 10.67TFIVSYY160 pKa = 10.64HH161 pKa = 4.93NVKK164 pKa = 10.92ANIEE168 pKa = 4.08RR169 pKa = 11.84LFQKK173 pKa = 10.56APEE176 pKa = 4.06PWVPPFPLSSFTNVPDD192 pKa = 5.1EE193 pKa = 4.14MQEE196 pKa = 3.79WADD199 pKa = 3.88DD200 pKa = 3.88YY201 pKa = 11.37FGRR204 pKa = 11.84DD205 pKa = 2.77AAARR209 pKa = 11.84PEE211 pKa = 4.08RR212 pKa = 11.84PISIIIEE219 pKa = 4.26GEE221 pKa = 4.01SRR223 pKa = 11.84TGKK226 pKa = 8.6TMWARR231 pKa = 11.84ALGPHH236 pKa = 6.63NYY238 pKa = 10.14LSGHH242 pKa = 6.9LDD244 pKa = 3.48FNSRR248 pKa = 11.84VYY250 pKa = 11.25SNEE253 pKa = 3.32AEE255 pKa = 4.23YY256 pKa = 11.29NVIDD260 pKa = 6.18DD261 pKa = 4.28ITPQYY266 pKa = 11.58LKK268 pKa = 9.91MKK270 pKa = 9.18HH271 pKa = 4.39WKK273 pKa = 9.63EE274 pKa = 3.81LIGAQKK280 pKa = 10.57DD281 pKa = 3.42WQSNCKK287 pKa = 9.38YY288 pKa = 10.21GKK290 pKa = 9.18PVQIKK295 pKa = 10.19GGIPSIVLCNPGEE308 pKa = 4.19GASYY312 pKa = 11.04KK313 pKa = 10.78SFLEE317 pKa = 4.04KK318 pKa = 10.97EE319 pKa = 4.07EE320 pKa = 4.04NKK322 pKa = 10.49ALKK325 pKa = 10.33DD326 pKa = 3.54WTLHH330 pKa = 4.48NAKK333 pKa = 10.41FIFLNSTLYY342 pKa = 10.11QAA344 pKa = 5.09

Molecular weight: 38.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9WHE6|Q9WHE6_9GEMI Capsid protein OS=Tomato severe leaf curl virus-[Guatemala 96-1] OX=223361 GN=cp PE=3 SV=2
MM1 pKa = 7.98PKK3 pKa = 9.65RR4 pKa = 11.84DD5 pKa = 3.82APWRR9 pKa = 11.84LMAGTPKK16 pKa = 10.2VSRR19 pKa = 11.84SSNQSPRR26 pKa = 11.84TGTGPKK32 pKa = 9.32FDD34 pKa = 4.92KK35 pKa = 10.85AHH37 pKa = 5.99AWVNRR42 pKa = 11.84PMYY45 pKa = 9.63RR46 pKa = 11.84KK47 pKa = 9.4PRR49 pKa = 11.84IYY51 pKa = 10.24RR52 pKa = 11.84AWRR55 pKa = 11.84TPDD58 pKa = 3.14VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 9.32VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.55VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.13TDD166 pKa = 3.03LRR168 pKa = 11.84DD169 pKa = 3.51RR170 pKa = 11.84YY171 pKa = 10.23QVMHH175 pKa = 6.91KK176 pKa = 10.25FYY178 pKa = 11.12GKK180 pKa = 8.61VTGGQYY186 pKa = 11.07ASNEE190 pKa = 3.69QAIVKK195 pKa = 9.07RR196 pKa = 11.84FWRR199 pKa = 11.84VNNHH203 pKa = 4.18VVYY206 pKa = 10.62NHH208 pKa = 5.8QEE210 pKa = 3.47AGKK213 pKa = 10.41YY214 pKa = 8.39EE215 pKa = 4.09NHH217 pKa = 6.52TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.66FYY246 pKa = 11.34DD247 pKa = 3.3SVLNN251 pKa = 4.02

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

855

128

344

213.8

24.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.316 ± 1.301

1.871 ± 0.428

5.146 ± 0.528

4.444 ± 0.769

4.327 ± 0.304

5.497 ± 0.663

4.094 ± 0.64

6.082 ± 0.915

6.55 ± 0.739

6.316 ± 0.9

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.988 ± 0.737

6.784 ± 0.363

5.965 ± 0.879

4.211 ± 0.933

6.316 ± 1.047

6.901 ± 1.082

4.912 ± 0.69

5.614 ± 1.517

1.871 ± 0.295

4.795 ± 0.84
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski