Antrodiella citrinella 
Average proteome isoelectric point is 6.23 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 9798 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A4S4MPP3|A0A4S4MPP3_9APHY HIG1 domain-containing protein OS=Antrodiella citrinella OX=2447956 GN=EUX98_g6970 PE=4 SV=1 
MM1 pKa = 7.21  PTASTDD7 pKa = 3.82  FTLEE11 pKa = 4.06  LPAMDD16 pKa = 5.15  SDD18 pKa = 6.14  LMPEE22 pKa = 3.96  WLYY25 pKa = 11.24  EE26 pKa = 4.19  PGPEE30 pKa = 4.86  IPLTQDD36 pKa = 3.0  DD37 pKa = 3.83  VRR39 pKa = 11.84  RR40 pKa = 11.84  LYY42 pKa = 11.02  RR43 pKa = 11.84  NSTCTTTEE51 pKa = 3.96  DD52 pKa = 4.2  SLSLGYY58 pKa = 10.79  GSIRR62 pKa = 11.84  APSPAEE68 pKa = 3.95  LQSLWDD74 pKa = 4.28  DD75 pKa = 4.07  SEE77 pKa = 5.05  SDD79 pKa = 3.57  SGSDD83 pKa = 4.96  DD84 pKa = 4.0  EE85 pKa = 5.8  PFDD88 pKa = 6.36  DD89 pKa = 5.99  EE90 pKa = 7.33  DD91 pKa = 5.94  DD92 pKa = 4.75  DD93 pKa = 5.71  EE94 pKa = 4.62  NLCVSDD100 pKa = 5.23  ADD102 pKa = 4.02  TDD104 pKa = 4.06  STSATLHH111 pKa = 6.54  DD112 pKa = 5.01  APVDD116 pKa = 4.02  PADD119 pKa = 4.27  HH120 pKa = 6.7  APSTSALKK128 pKa = 10.22  QPQLISPPSPPSPRR142 pKa = 11.84  RR143 pKa = 11.84  HH144 pKa = 6.36  PDD146 pKa = 3.14  DD147 pKa = 4.29  LLRR150 pKa = 11.84  LYY152 pKa = 10.04  AYY154 pKa = 10.7  SSADD158 pKa = 3.48  PDD160 pKa = 4.66  LATCLHH166 pKa = 6.9  GIDD169 pKa = 4.78  EE170 pKa = 5.14  ALLCNPFSVYY180 pKa = 11.26  ADD182 pKa = 3.36  VCYY185 pKa = 10.48  NAAVKK190 pKa = 10.47  RR191 pKa = 11.84  EE192 pKa = 4.01  PQNGGVSRR200 pKa = 11.84  GSNEE204 pKa = 3.79  LEE206 pKa = 3.7  RR207 pKa = 11.84  VTEE210 pKa = 3.87  EE211 pKa = 5.06  DD212 pKa = 3.21  EE213 pKa = 4.39  TEE215 pKa = 3.96  EE216 pKa = 4.45  AEE218 pKa = 4.37  SEE220 pKa = 4.14  EE221 pKa = 5.28  SEE223 pKa = 5.71  DD224 pKa = 3.29  EE225 pKa = 4.33  DD226 pKa = 4.75  EE227 pKa = 5.03  PVLHH231 pKa = 6.97  SPIQTGVVASNWTSGVYY248 pKa = 9.82  VGNAPSEE255 pKa = 4.18  ALSALALPAPYY266 pKa = 9.64  HH267 pKa = 5.81  ATMDD271 pKa = 3.88  SKK273 pKa = 10.87  PGCFVSPVSTNN284 pKa = 2.84   
 Molecular weight: 30.62 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.693 
IPC2_protein 3.757 
IPC_protein 3.77 
Toseland    3.554 
ProMoST     3.923 
Dawson      3.757 
Bjellqvist  3.91 
Wikipedia   3.681 
Rodwell     3.592 
Grimsley    3.465 
Solomon     3.757 
Lehninger   3.706 
Nozaki      3.872 
DTASelect   4.088 
Thurlkill   3.605 
EMBOSS      3.694 
Sillero     3.897 
Patrickios  1.265 
IPC_peptide 3.745 
IPC2_peptide  3.872 
IPC2.peptide.svr19  3.795 
 Protein with the highest isoelectric point: 
>tr|A0A4S4MVL2|A0A4S4MVL2_9APHY Signal recognition particle receptor subunit beta OS=Antrodiella citrinella OX=2447956 GN=EUX98_g3829 PE=3 SV=1 
MM1 pKa = 6.8  THH3 pKa = 6.6  DD4 pKa = 5.65  AIWFSRR10 pKa = 11.84  PRR12 pKa = 11.84  KK13 pKa = 7.98  YY14 pKa = 11.02  GKK16 pKa = 10.34  GSRR19 pKa = 11.84  QCRR22 pKa = 11.84  LCAHH26 pKa = 5.84  QAGLIRR32 pKa = 11.84  KK33 pKa = 9.09  YY34 pKa = 10.91  GIDD37 pKa = 3.51  LCRR40 pKa = 11.84  QCFRR44 pKa = 11.84  EE45 pKa = 4.02  KK46 pKa = 10.43  SAAIGFTKK54 pKa = 10.0  TRR56 pKa = 3.53   
 Molecular weight: 6.52 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.351 
IPC2_protein 9.604 
IPC_protein 10.131 
Toseland    10.584 
ProMoST     10.511 
Dawson      10.687 
Bjellqvist  10.394 
Wikipedia   10.862 
Rodwell     11.023 
Grimsley    10.73 
Solomon     10.774 
Lehninger   10.76 
Nozaki      10.628 
DTASelect   10.365 
Thurlkill   10.584 
EMBOSS      10.979 
Sillero     10.628 
Patrickios  10.804 
IPC_peptide 10.789 
IPC2_peptide  9.677 
IPC2.peptide.svr19  8.599 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        9798 
 
        
        0
 
        
        9798 
         
        4715391
 
        28
 
        4919
 
        481.3
 
        53.14
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.829 ± 0.02
1.08 ± 0.008
 
        6.015 ± 0.019
6.098 ± 0.024
 
        3.703 ± 0.015
6.339 ± 0.02
 
        2.536 ± 0.011
4.761 ± 0.017
       
        4.703 ± 0.021
9.026 ± 0.027
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.155 ± 0.008
3.378 ± 0.014
 
        6.413 ± 0.028
3.75 ± 0.016
 
        5.972 ± 0.021
8.341 ± 0.029
 
        6.118 ± 0.015
6.671 ± 0.019
       
        1.373 ± 0.007
2.678 ± 0.011
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here