Tortoise microvirus 43

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W792|A0A4P8W792_9VIRU Replication initiation protein OS=Tortoise microvirus 43 OX=2583147 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.2RR3 pKa = 11.84QRR5 pKa = 11.84EE6 pKa = 4.14EE7 pKa = 4.05TGGYY11 pKa = 7.66TYY13 pKa = 10.77SGSSSEE19 pKa = 3.98YY20 pKa = 9.54SAYY23 pKa = 10.01EE24 pKa = 4.01AYY26 pKa = 10.2QKK28 pKa = 11.4GLIGGGGNGGTGKK41 pKa = 8.23TVTAGPGVDD50 pKa = 3.72GAQTVPVGEE59 pKa = 4.46PVAEE63 pKa = 4.15VAPVTQQTPPAPVTGPGGPRR83 pKa = 11.84RR84 pKa = 11.84QSTLLSPLEE93 pKa = 3.92PMGPYY98 pKa = 9.97GPRR101 pKa = 11.84IQRR104 pKa = 11.84EE105 pKa = 4.68GTRR108 pKa = 11.84LTPKK112 pKa = 9.8MEE114 pKa = 4.1MSVQEE119 pKa = 4.2MLVDD123 pKa = 4.55GYY125 pKa = 10.91RR126 pKa = 11.84INANPRR132 pKa = 11.84TSNAEE137 pKa = 3.57EE138 pKa = 3.99WEE140 pKa = 3.87IRR142 pKa = 11.84YY143 pKa = 9.65AEE145 pKa = 4.4PGEE148 pKa = 4.15WVGGIVVMASDD159 pKa = 3.07AWYY162 pKa = 7.99NTKK165 pKa = 10.07QALGDD170 pKa = 3.73LSYY173 pKa = 11.19KK174 pKa = 10.83ANAANNSWAGATHH187 pKa = 7.07AGDD190 pKa = 5.36AFGDD194 pKa = 3.69WFDD197 pKa = 4.58SRR199 pKa = 11.84LQSVSPKK206 pKa = 10.44AGEE209 pKa = 4.07LVQEE213 pKa = 4.21PVDD216 pKa = 3.31WGRR219 pKa = 3.47

Molecular weight: 23.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9S0|A0A4P8W9S0_9VIRU Major capsid protein OS=Tortoise microvirus 43 OX=2583147 PE=4 SV=1
MM1 pKa = 7.89ANRR4 pKa = 11.84TGNTSKK10 pKa = 10.95EE11 pKa = 4.07KK12 pKa = 10.89AEE14 pKa = 3.72RR15 pKa = 11.84AARR18 pKa = 11.84NTSSASSGSRR28 pKa = 11.84TSSSSRR34 pKa = 11.84SNSGTSKK41 pKa = 10.67ASSGSKK47 pKa = 10.21GVSTPRR53 pKa = 11.84TSSRR57 pKa = 11.84QSAPRR62 pKa = 11.84APDD65 pKa = 3.36PVSRR69 pKa = 11.84QSSRR73 pKa = 11.84ATARR77 pKa = 11.84ADD79 pKa = 3.61ASRR82 pKa = 11.84GDD84 pKa = 4.02HH85 pKa = 5.93GWNDD89 pKa = 2.96SWAGASTDD97 pKa = 3.45NGSIGSNSGSDD108 pKa = 3.77YY109 pKa = 11.33GWGNAKK115 pKa = 10.22KK116 pKa = 9.74KK117 pKa = 8.72VTGGTPTSPSPSLPSPTAPDD137 pKa = 3.42PVEE140 pKa = 3.99TRR142 pKa = 11.84KK143 pKa = 9.85SAKK146 pKa = 8.9ATNLRR151 pKa = 11.84IAPTRR156 pKa = 11.84EE157 pKa = 3.85TLPHH161 pKa = 6.75KK162 pKa = 10.01EE163 pKa = 3.72RR164 pKa = 11.84SPARR168 pKa = 11.84QEE170 pKa = 3.87ARR172 pKa = 11.84AKK174 pKa = 10.11RR175 pKa = 11.84STPIEE180 pKa = 3.98PAKK183 pKa = 10.28QSPSPKK189 pKa = 9.39VRR191 pKa = 11.84EE192 pKa = 3.89EE193 pKa = 4.02KK194 pKa = 10.79KK195 pKa = 10.32PVLHH199 pKa = 6.52CKK201 pKa = 9.52PRR203 pKa = 11.84PKK205 pKa = 10.4DD206 pKa = 3.6NKK208 pKa = 8.18PTGGGGGGGNFKK220 pKa = 10.72KK221 pKa = 10.68FIPWCGG227 pKa = 3.05

Molecular weight: 23.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1693

89

539

241.9

26.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.451 ± 1.037

0.413 ± 0.19

5.08 ± 0.601

7.324 ± 0.746

2.953 ± 0.694

8.683 ± 1.145

2.304 ± 0.527

3.131 ± 0.418

5.67 ± 1.135

6.852 ± 1.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.478

3.249 ± 0.745

7.147 ± 0.888

4.194 ± 0.736

7.915 ± 0.724

6.615 ± 1.991

5.316 ± 1.101

6.143 ± 0.894

1.949 ± 0.409

3.131 ± 0.787
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski