Streptomyces phage IchabodCrane

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Karimacvirus; unclassified Karimacvirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8DEC4|A0A5P8DEC4_9CAUD Uncharacterized protein OS=Streptomyces phage IchabodCrane OX=2652898 GN=26 PE=4 SV=1
MM1 pKa = 7.48NIDD4 pKa = 3.87DD5 pKa = 6.42LIEE8 pKa = 4.1EE9 pKa = 4.25WHH11 pKa = 6.9EE12 pKa = 4.24GDD14 pKa = 4.8SPLPLHH20 pKa = 6.79EE21 pKa = 4.39FLGMTSEE28 pKa = 4.44EE29 pKa = 3.84YY30 pKa = 10.95DD31 pKa = 3.5LFVRR35 pKa = 11.84FGTLPEE41 pKa = 5.11GITYY45 pKa = 10.7GDD47 pKa = 3.54SS48 pKa = 3.12

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8DEJ2|A0A5P8DEJ2_9CAUD HNH endonuclease OS=Streptomyces phage IchabodCrane OX=2652898 GN=98 PE=4 SV=1
MM1 pKa = 7.42ALVGNSNTCWFQRR14 pKa = 11.84CGNKK18 pKa = 9.45PIHH21 pKa = 7.29RR22 pKa = 11.84IRR24 pKa = 11.84YY25 pKa = 6.78YY26 pKa = 10.09NKK28 pKa = 9.42TVMVCNKK35 pKa = 9.76HH36 pKa = 6.39KK37 pKa = 10.88NLDD40 pKa = 3.8GAGCAPSRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84PKK52 pKa = 10.83GSAA55 pKa = 2.9

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

214

0

214

33830

34

2099

158.1

17.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.46 ± 0.338

1.052 ± 0.114

6.426 ± 0.178

7.026 ± 0.352

3.804 ± 0.127

7.467 ± 0.232

1.75 ± 0.109

5.436 ± 0.148

6.61 ± 0.329

7.21 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.042 ± 0.134

4.611 ± 0.148

3.586 ± 0.157

3.263 ± 0.197

5.542 ± 0.191

5.862 ± 0.259

6.113 ± 0.396

6.988 ± 0.202

1.96 ± 0.09

3.792 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski