Proteome-pI 2.0 - Proteome Isoelectric Point Database

Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Desulfuromonadaceae; Pelobacter; Pelobacter propionicus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A1AUP5|A1AUP5_PELPD Cobyrinic acid a c-diamide synthase family protein OS=Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1) OX=338966 GN=Ppro_3473 PE=4 SV=1

MM1 pKa = 6.88ITSITSATDD10 pKa = 3.15STTTSAAIKK19 pKa = 10.21SSLGMDD25 pKa = 3.18SSDD28 pKa = 3.47FLQLFIAQMQYY39 pKa = 10.93QDD41 pKa = 4.24PLAPTDD47 pKa = 3.46ATAMMNQLSQLSLVEE62 pKa = 3.61QSYY65 pKa = 10.13NQTTALSNLLAAQNNSLSMGSVSFIGKK92 pKa = 5.86TVKK95 pKa = 10.81ANGDD99 pKa = 3.98DD100 pKa = 3.38IVFDD104 pKa = 4.11GTSSPSLQYY113 pKa = 11.06NLSSASAATTLTISDD128 pKa = 3.76SSGEE132 pKa = 4.22SVRR135 pKa = 11.84TVSLGAQSTGDD146 pKa = 4.21NSYY149 pKa = 8.19TWDD152 pKa = 3.64GCDD155 pKa = 3.18NSGNKK160 pKa = 9.72LSAGAYY166 pKa = 6.09TFSVSAKK173 pKa = 8.16TGSGASVTAKK183 pKa = 9.9TYY185 pKa = 5.96TTGVIDD191 pKa = 4.25GVNLTSTTPYY201 pKa = 10.75LSIGTVSVPLTDD213 pKa = 3.46VLNVSGAA220 pKa = 3.27

Molecular weight: 22.49 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0R7L9|A0R7L9_PELPD Uncharacterized protein OS=Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1) OX=338966 GN=Ppro_3637 PE=4 SV=1

MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTSRR12 pKa = 11.84KK13 pKa = 7.49RR14 pKa = 11.84THH16 pKa = 6.14GFLVRR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.01GRR39 pKa = 11.84KK40 pKa = 8.16NLAVRR45 pKa = 11.84IATKK49 pKa = 10.56

Molecular weight: 5.74 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3694	0	3694	1202724	37	2954	325.6	35.99

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.259 ± 0.047	1.374 ± 0.017	5.265 ± 0.032	6.461 ± 0.042	3.927 ± 0.026	7.858 ± 0.039	2.072 ± 0.018	5.959 ± 0.034	4.285 ± 0.041	10.633 ± 0.052

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.632 ± 0.018	3.251 ± 0.031	4.547 ± 0.028	3.402 ± 0.024	6.964 ± 0.042	6.25 ± 0.032	5.214 ± 0.033	6.937 ± 0.033	1.042 ± 0.014	2.669 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski