Amino acid dipepetide frequency for Gammaproteobacteria bacterium MFB021

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.233AlaAla: 15.233 ± 0.176	1.269AlaCys: 1.269 ± 0.035	6.51AlaAsp: 6.51 ± 0.086	7.607AlaGlu: 7.607 ± 0.098	4.117AlaPhe: 4.117 ± 0.075	9.976AlaGly: 9.976 ± 0.104	2.561AlaHis: 2.561 ± 0.054	5.654AlaIle: 5.654 ± 0.079	2.971AlaLys: 2.971 ± 0.071	15.415AlaLeu: 15.415 ± 0.161	3.2AlaMet: 3.2 ± 0.049	2.777AlaAsn: 2.777 ± 0.056	5.298AlaPro: 5.298 ± 0.077	4.719AlaGln: 4.719 ± 0.083	8.942AlaArg: 8.942 ± 0.122	6.586AlaSer: 6.586 ± 0.084	6.013AlaThr: 6.013 ± 0.082	7.64AlaVal: 7.64 ± 0.095	1.997AlaTrp: 1.997 ± 0.049	2.523AlaTyr: 2.523 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.013CysAla: 1.013 ± 0.031	0.152CysCys: 0.152 ± 0.012	0.543CysAsp: 0.543 ± 0.024	0.532CysGlu: 0.532 ± 0.023	0.299CysPhe: 0.299 ± 0.017	0.984CysGly: 0.984 ± 0.033	0.336CysHis: 0.336 ± 0.016	0.347CysIle: 0.347 ± 0.019	0.169CysLys: 0.169 ± 0.015	0.985CysLeu: 0.985 ± 0.031	0.163CysMet: 0.163 ± 0.013	0.211CysAsn: 0.211 ± 0.014	0.47CysPro: 0.47 ± 0.021	0.39CysGln: 0.39 ± 0.018	0.767CysArg: 0.767 ± 0.03	0.475CysSer: 0.475 ± 0.025	0.391CysThr: 0.391 ± 0.02	0.693CysVal: 0.693 ± 0.029	0.139CysTrp: 0.139 ± 0.011	0.257CysTyr: 0.257 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	7.494AspAla: 7.494 ± 0.096	0.461AspCys: 0.461 ± 0.023	3.99AspAsp: 3.99 ± 0.08	3.516AspGlu: 3.516 ± 0.065	2.146AspPhe: 2.146 ± 0.053	5.026AspGly: 5.026 ± 0.09	1.377AspHis: 1.377 ± 0.037	2.761AspIle: 2.761 ± 0.053	1.654AspLys: 1.654 ± 0.049	5.456AspLeu: 5.456 ± 0.072	1.187AspMet: 1.187 ± 0.033	1.607AspAsn: 1.607 ± 0.042	3.244AspPro: 3.244 ± 0.059	2.037AspGln: 2.037 ± 0.046	3.492AspArg: 3.492 ± 0.058	3.009AspSer: 3.009 ± 0.055	3.135AspThr: 3.135 ± 0.072	3.891AspVal: 3.891 ± 0.063	0.995AspTrp: 0.995 ± 0.034	1.899AspTyr: 1.899 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	7.806GluAla: 7.806 ± 0.096	0.484GluCys: 0.484 ± 0.023	2.464GluAsp: 2.464 ± 0.057	3.039GluGlu: 3.039 ± 0.071	1.586GluPhe: 1.586 ± 0.038	4.255GluGly: 4.255 ± 0.074	1.519GluHis: 1.519 ± 0.041	3.094GluIle: 3.094 ± 0.064	1.636GluLys: 1.636 ± 0.047	6.216GluLeu: 6.216 ± 0.081	1.423GluMet: 1.423 ± 0.038	1.329GluAsn: 1.329 ± 0.039	2.602GluPro: 2.602 ± 0.06	2.842GluGln: 2.842 ± 0.055	6.024GluArg: 6.024 ± 0.097	3.18GluSer: 3.18 ± 0.053	3.261GluThr: 3.261 ± 0.054	4.176GluVal: 4.176 ± 0.074	0.86GluTrp: 0.86 ± 0.028	1.14GluTyr: 1.14 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.914PheAla: 3.914 ± 0.062	0.373PheCys: 0.373 ± 0.018	2.702PheAsp: 2.702 ± 0.056	2.108PheGlu: 2.108 ± 0.053	1.297PhePhe: 1.297 ± 0.042	3.271PheGly: 3.271 ± 0.06	0.803PheHis: 0.803 ± 0.025	1.575PheIle: 1.575 ± 0.04	0.884PheLys: 0.884 ± 0.03	2.995PheLeu: 2.995 ± 0.071	0.816PheMet: 0.816 ± 0.027	1.001PheAsn: 1.001 ± 0.033	1.398PhePro: 1.398 ± 0.039	1.126PheGln: 1.126 ± 0.031	1.848PheArg: 1.848 ± 0.043	2.23PheSer: 2.23 ± 0.052	1.929PheThr: 1.929 ± 0.048	2.613PheVal: 2.613 ± 0.059	0.489PheTrp: 0.489 ± 0.024	0.955PheTyr: 0.955 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	8.266GlyAla: 8.266 ± 0.102	0.967GlyCys: 0.967 ± 0.039	4.757GlyAsp: 4.757 ± 0.084	5.596GlyGlu: 5.596 ± 0.078	3.249GlyPhe: 3.249 ± 0.055	7.181GlyGly: 7.181 ± 0.115	2.049GlyHis: 2.049 ± 0.046	4.369GlyIle: 4.369 ± 0.069	2.643GlyLys: 2.643 ± 0.059	9.238GlyLeu: 9.238 ± 0.103	2.388GlyMet: 2.388 ± 0.056	2.104GlyAsn: 2.104 ± 0.056	2.847GlyPro: 2.847 ± 0.047	3.37GlyGln: 3.37 ± 0.067	5.547GlyArg: 5.547 ± 0.084	4.573GlySer: 4.573 ± 0.077	3.827GlyThr: 3.827 ± 0.087	6.768GlyVal: 6.768 ± 0.074	1.501GlyTrp: 1.501 ± 0.039	2.469GlyTyr: 2.469 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.902HisAla: 2.902 ± 0.06	0.294HisCys: 0.294 ± 0.015	1.47HisAsp: 1.47 ± 0.037	1.197HisGlu: 1.197 ± 0.034	0.962HisPhe: 0.962 ± 0.029	2.235HisGly: 2.235 ± 0.053	0.744HisHis: 0.744 ± 0.031	0.89HisIle: 0.89 ± 0.028	0.51HisLys: 0.51 ± 0.023	2.534HisLeu: 2.534 ± 0.053	0.446HisMet: 0.446 ± 0.022	0.537HisAsn: 0.537 ± 0.021	1.525HisPro: 1.525 ± 0.044	0.977HisGln: 0.977 ± 0.031	1.715HisArg: 1.715 ± 0.046	1.064HisSer: 1.064 ± 0.034	1.05HisThr: 1.05 ± 0.032	1.459HisVal: 1.459 ± 0.039	0.473HisTrp: 0.473 ± 0.024	0.843HisTyr: 0.843 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.207IleAla: 6.207 ± 0.083	0.431IleCys: 0.431 ± 0.02	3.741IleAsp: 3.741 ± 0.061	3.379IleGlu: 3.379 ± 0.069	1.361IlePhe: 1.361 ± 0.036	4.645IleGly: 4.645 ± 0.066	0.98IleHis: 0.98 ± 0.032	1.774IleIle: 1.774 ± 0.049	1.19IleLys: 1.19 ± 0.038	3.788IleLeu: 3.788 ± 0.079	0.813IleMet: 0.813 ± 0.033	1.427IleAsn: 1.427 ± 0.039	2.16IlePro: 2.16 ± 0.056	1.52IleGln: 1.52 ± 0.039	2.887IleArg: 2.887 ± 0.05	2.562IleSer: 2.562 ± 0.045	2.586IleThr: 2.586 ± 0.057	3.626IleVal: 3.626 ± 0.061	0.451IleTrp: 0.451 ± 0.021	1.066IleTyr: 1.066 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.075LysAla: 3.075 ± 0.07	0.134LysCys: 0.134 ± 0.012	1.162LysAsp: 1.162 ± 0.035	1.206LysGlu: 1.206 ± 0.043	0.573LysPhe: 0.573 ± 0.026	1.984LysGly: 1.984 ± 0.048	0.554LysHis: 0.554 ± 0.023	1.197LysIle: 1.197 ± 0.035	0.84LysLys: 0.84 ± 0.038	2.908LysLeu: 2.908 ± 0.061	0.555LysMet: 0.555 ± 0.021	0.577LysAsn: 0.577 ± 0.025	1.511LysPro: 1.511 ± 0.051	1.227LysGln: 1.227 ± 0.041	2.385LysArg: 2.385 ± 0.048	1.489LysSer: 1.489 ± 0.038	1.335LysThr: 1.335 ± 0.036	1.92LysVal: 1.92 ± 0.045	0.262LysTrp: 0.262 ± 0.014	0.522LysTyr: 0.522 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	15.592LeuAla: 15.592 ± 0.165	1.105LeuCys: 1.105 ± 0.034	7.472LeuAsp: 7.472 ± 0.1	6.745LeuGlu: 6.745 ± 0.092	3.772LeuPhe: 3.772 ± 0.072	9.67LeuGly: 9.67 ± 0.112	2.327LeuHis: 2.327 ± 0.053	5.566LeuIle: 5.566 ± 0.089	3.249LeuLys: 3.249 ± 0.065	11.876LeuLeu: 11.876 ± 0.177	2.629LeuMet: 2.629 ± 0.055	2.771LeuAsn: 2.771 ± 0.05	6.029LeuPro: 6.029 ± 0.081	3.041LeuGln: 3.041 ± 0.052	6.867LeuArg: 6.867 ± 0.089	6.878LeuSer: 6.878 ± 0.093	6.06LeuThr: 6.06 ± 0.083	7.728LeuVal: 7.728 ± 0.107	1.471LeuTrp: 1.471 ± 0.045	2.501LeuTyr: 2.501 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.975MetAla: 2.975 ± 0.06	0.138MetCys: 0.138 ± 0.011	0.997MetAsp: 0.997 ± 0.033	0.944MetGlu: 0.944 ± 0.03	0.613MetPhe: 0.613 ± 0.027	1.738MetGly: 1.738 ± 0.046	0.498MetHis: 0.498 ± 0.02	1.201MetIle: 1.201 ± 0.035	0.755MetLys: 0.755 ± 0.027	2.628MetLeu: 2.628 ± 0.053	0.629MetMet: 0.629 ± 0.031	0.704MetAsn: 0.704 ± 0.031	1.384MetPro: 1.384 ± 0.036	1.064MetGln: 1.064 ± 0.03	1.647MetArg: 1.647 ± 0.041	1.753MetSer: 1.753 ± 0.044	1.591MetThr: 1.591 ± 0.039	1.548MetVal: 1.548 ± 0.041	0.178MetTrp: 0.178 ± 0.014	0.297MetTyr: 0.297 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.14AsnAla: 3.14 ± 0.066	0.207AsnCys: 0.207 ± 0.014	1.488AsnAsp: 1.488 ± 0.046	1.116AsnGlu: 1.116 ± 0.03	0.816AsnPhe: 0.816 ± 0.032	2.181AsnGly: 2.181 ± 0.059	0.568AsnHis: 0.568 ± 0.026	1.073AsnIle: 1.073 ± 0.033	0.514AsnLys: 0.514 ± 0.023	2.671AsnLeu: 2.671 ± 0.06	0.508AsnMet: 0.508 ± 0.021	0.631AsnAsn: 0.631 ± 0.026	1.604AsnPro: 1.604 ± 0.046	0.919AsnGln: 0.919 ± 0.027	1.752AsnArg: 1.752 ± 0.046	1.088AsnSer: 1.088 ± 0.034	1.206AsnThr: 1.206 ± 0.036	1.824AsnVal: 1.824 ± 0.045	0.339AsnTrp: 0.339 ± 0.019	0.629AsnTyr: 0.629 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	6.029ProAla: 6.029 ± 0.096	0.369ProCys: 0.369 ± 0.02	3.075ProAsp: 3.075 ± 0.059	3.531ProGlu: 3.531 ± 0.054	1.711ProPhe: 1.711 ± 0.037	4.346ProGly: 4.346 ± 0.07	1.121ProHis: 1.121 ± 0.031	2.091ProIle: 2.091 ± 0.043	1.179ProLys: 1.179 ± 0.036	5.694ProLeu: 5.694 ± 0.082	1.142ProMet: 1.142 ± 0.036	1.166ProAsn: 1.166 ± 0.031	2.232ProPro: 2.232 ± 0.058	2.067ProGln: 2.067 ± 0.044	3.211ProArg: 3.211 ± 0.064	2.618ProSer: 2.618 ± 0.054	2.384ProThr: 2.384 ± 0.049	3.523ProVal: 3.523 ± 0.059	0.786ProTrp: 0.786 ± 0.029	1.174ProTyr: 1.174 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.617GlnAla: 5.617 ± 0.086	0.284GlnCys: 0.284 ± 0.018	1.571GlnAsp: 1.571 ± 0.037	1.85GlnGlu: 1.85 ± 0.043	1.11GlnPhe: 1.11 ± 0.037	3.085GlnGly: 3.085 ± 0.056	0.944GlnHis: 0.944 ± 0.035	1.757GlnIle: 1.757 ± 0.038	0.903GlnLys: 0.903 ± 0.029	4.426GlnLeu: 4.426 ± 0.069	0.842GlnMet: 0.842 ± 0.028	0.816GlnAsn: 0.816 ± 0.028	2.198GlnPro: 2.198 ± 0.049	2.223GlnGln: 2.223 ± 0.063	3.999GlnArg: 3.999 ± 0.076	2.04GlnSer: 2.04 ± 0.041	1.951GlnThr: 1.951 ± 0.048	2.828GlnVal: 2.828 ± 0.061	0.644GlnTrp: 0.644 ± 0.026	0.775GlnTyr: 0.775 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	7.338ArgAla: 7.338 ± 0.088	0.682ArgCys: 0.682 ± 0.026	4.339ArgAsp: 4.339 ± 0.071	5.331ArgGlu: 5.331 ± 0.073	3.04ArgPhe: 3.04 ± 0.055	5.02ArgGly: 5.02 ± 0.072	2.273ArgHis: 2.273 ± 0.054	3.339ArgIle: 3.339 ± 0.069	1.55ArgLys: 1.55 ± 0.046	9.537ArgLeu: 9.537 ± 0.128	1.596ArgMet: 1.596 ± 0.036	1.461ArgAsn: 1.461 ± 0.044	3.047ArgPro: 3.047 ± 0.056	3.777ArgGln: 3.777 ± 0.079	6.096ArgArg: 6.096 ± 0.101	3.026ArgSer: 3.026 ± 0.064	2.793ArgThr: 2.793 ± 0.057	5.254ArgVal: 5.254 ± 0.073	1.243ArgTrp: 1.243 ± 0.037	2.344ArgTyr: 2.344 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	6.295SerAla: 6.295 ± 0.085	0.393SerCys: 0.393 ± 0.021	3.256SerAsp: 3.256 ± 0.063	3.014SerGlu: 3.014 ± 0.051	1.873SerPhe: 1.873 ± 0.037	5.409SerGly: 5.409 ± 0.083	1.326SerHis: 1.326 ± 0.039	2.277SerIle: 2.277 ± 0.048	1.113SerLys: 1.113 ± 0.038	6.35SerLeu: 6.35 ± 0.088	1.309SerMet: 1.309 ± 0.038	1.229SerAsn: 1.229 ± 0.036	2.821SerPro: 2.821 ± 0.053	2.267SerGln: 2.267 ± 0.047	4.121SerArg: 4.121 ± 0.063	2.8SerSer: 2.8 ± 0.068	2.49SerThr: 2.49 ± 0.048	3.852SerVal: 3.852 ± 0.069	0.773SerTrp: 0.773 ± 0.025	1.298SerTyr: 1.298 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	5.252ThrAla: 5.252 ± 0.077	0.418ThrCys: 0.418 ± 0.021	2.426ThrAsp: 2.426 ± 0.059	2.143ThrGlu: 2.143 ± 0.045	1.926ThrPhe: 1.926 ± 0.051	4.187ThrGly: 4.187 ± 0.087	1.255ThrHis: 1.255 ± 0.034	2.094ThrIle: 2.094 ± 0.054	0.854ThrLys: 0.854 ± 0.033	8.046ThrLeu: 8.046 ± 0.088	0.902ThrMet: 0.902 ± 0.034	1.122ThrAsn: 1.122 ± 0.039	3.792ThrPro: 3.792 ± 0.062	2.112ThrGln: 2.112 ± 0.044	3.814ThrArg: 3.814 ± 0.068	2.583ThrSer: 2.583 ± 0.048	2.61ThrThr: 2.61 ± 0.056	3.253ThrVal: 3.253 ± 0.074	0.673ThrTrp: 0.673 ± 0.026	1.136ThrTyr: 1.136 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	8.85ValAla: 8.85 ± 0.098	0.717ValCys: 0.717 ± 0.026	4.433ValAsp: 4.433 ± 0.069	4.427ValGlu: 4.427 ± 0.073	2.409ValPhe: 2.409 ± 0.052	5.539ValGly: 5.539 ± 0.084	1.433ValHis: 1.433 ± 0.037	3.996ValIle: 3.996 ± 0.06	1.968ValLys: 1.968 ± 0.049	7.249ValLeu: 7.249 ± 0.114	1.919ValMet: 1.919 ± 0.045	1.855ValAsn: 1.855 ± 0.046	3.261ValPro: 3.261 ± 0.058	2.021ValGln: 2.021 ± 0.042	4.247ValArg: 4.247 ± 0.072	4.288ValSer: 4.288 ± 0.058	4.101ValThr: 4.101 ± 0.067	5.859ValVal: 5.859 ± 0.093	0.904ValTrp: 0.904 ± 0.029	1.564ValTyr: 1.564 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.175TrpAla: 1.175 ± 0.037	0.157TrpCys: 0.157 ± 0.014	0.594TrpAsp: 0.594 ± 0.022	0.666TrpGlu: 0.666 ± 0.027	0.54TrpPhe: 0.54 ± 0.025	0.94TrpGly: 0.94 ± 0.033	0.467TrpHis: 0.467 ± 0.02	0.692TrpIle: 0.692 ± 0.029	0.346TrpLys: 0.346 ± 0.014	2.421TrpLeu: 2.421 ± 0.057	0.401TrpMet: 0.401 ± 0.02	0.374TrpAsn: 0.374 ± 0.018	0.723TrpPro: 0.723 ± 0.029	1.037TrpGln: 1.037 ± 0.034	1.332TrpArg: 1.332 ± 0.045	0.784TrpSer: 0.784 ± 0.031	0.625TrpThr: 0.625 ± 0.027	0.966TrpVal: 0.966 ± 0.03	0.274TrpTrp: 0.274 ± 0.015	0.332TrpTyr: 0.332 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.834TyrAla: 2.834 ± 0.053	0.282TyrCys: 0.282 ± 0.017	1.385TyrAsp: 1.385 ± 0.043	0.992TyrGlu: 0.992 ± 0.031	0.925TyrPhe: 0.925 ± 0.03	2.04TyrGly: 2.04 ± 0.048	0.692TyrHis: 0.692 ± 0.027	0.845TyrIle: 0.845 ± 0.028	0.487TyrLys: 0.487 ± 0.023	2.915TyrLeu: 2.915 ± 0.056	0.414TyrMet: 0.414 ± 0.022	0.573TyrAsn: 0.573 ± 0.022	1.302TyrPro: 1.302 ± 0.034	1.127TyrGln: 1.127 ± 0.035	2.392TyrArg: 2.392 ± 0.054	1.239TyrSer: 1.239 ± 0.04	1.261TyrThr: 1.261 ± 0.037	1.619TyrVal: 1.619 ± 0.034	0.396TyrTrp: 0.396 ± 0.021	0.715TyrTyr: 0.715 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3307 proteins (1054741 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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