Paenibacillus sp. 7516

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5798 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A268SHU6|A0A268SHU6_9BACL Mismatch-specific DNA-glycosylase OS=Paenibacillus sp. 7516 OX=2022549 GN=CHI14_13055 PE=4 SV=1
MM1 pKa = 7.23EE2 pKa = 4.22TTVCPWCHH10 pKa = 5.9TEE12 pKa = 4.2IVWDD16 pKa = 3.89EE17 pKa = 3.92EE18 pKa = 4.19LGPEE22 pKa = 4.22EE23 pKa = 4.24EE24 pKa = 5.69CPYY27 pKa = 10.62CHH29 pKa = 7.08NEE31 pKa = 3.65LKK33 pKa = 10.51GYY35 pKa = 7.59RR36 pKa = 11.84TLNINIGEE44 pKa = 4.25EE45 pKa = 3.91EE46 pKa = 4.74DD47 pKa = 3.6EE48 pKa = 5.53DD49 pKa = 4.34EE50 pKa = 5.14VSDD53 pKa = 5.25IYY55 pKa = 11.58DD56 pKa = 3.13VDD58 pKa = 4.08DD59 pKa = 5.41AQDD62 pKa = 3.49SDD64 pKa = 3.78QTTDD68 pKa = 5.11LAALWGDD75 pKa = 3.6EE76 pKa = 4.45VEE78 pKa = 4.96LKK80 pKa = 10.89LPEE83 pKa = 4.46LRR85 pKa = 11.84TLTKK89 pKa = 10.79YY90 pKa = 11.1ADD92 pKa = 4.43DD93 pKa = 4.69GNDD96 pKa = 3.64LILYY100 pKa = 9.3EE101 pKa = 4.46EE102 pKa = 4.75GVEE105 pKa = 4.2KK106 pKa = 10.76QLDD109 pKa = 3.82LQEE112 pKa = 4.68EE113 pKa = 4.86VPEE116 pKa = 4.64CPHH119 pKa = 6.5CRR121 pKa = 11.84EE122 pKa = 3.86FMILAGTQTSTKK134 pKa = 10.07DD135 pKa = 2.88TFTPVANAVSRR146 pKa = 11.84TDD148 pKa = 3.45SLLDD152 pKa = 3.53APFTVQMYY160 pKa = 9.29VCSGCFQVQYY170 pKa = 11.07SLSEE174 pKa = 4.17DD175 pKa = 3.27DD176 pKa = 5.17RR177 pKa = 11.84LRR179 pKa = 11.84LIQNLSAKK187 pKa = 10.02KK188 pKa = 10.17

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A268S8A4|A0A268S8A4_9BACL Uncharacterized protein OS=Paenibacillus sp. 7516 OX=2022549 GN=CHI14_26255 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.64VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5798

0

5798

1863486

26

2471

321.4

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.947 ± 0.034

0.721 ± 0.009

5.211 ± 0.02

6.905 ± 0.039

3.997 ± 0.024

7.378 ± 0.033

2.226 ± 0.019

6.661 ± 0.032

5.023 ± 0.025

9.955 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.006 ± 0.016

3.889 ± 0.023

4.01 ± 0.021

4.161 ± 0.022

4.938 ± 0.03

6.426 ± 0.029

5.573 ± 0.029

7.116 ± 0.026

1.328 ± 0.015

3.529 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski