Amino acid dipepetide frequency for Bacillus phage vB_BtS_BMBtp16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.231AlaAla: 5.231 ± 1.316
0.214AlaCys: 0.214 ± 0.14
3.737AlaAsp: 3.737 ± 0.619
5.018AlaGlu: 5.018 ± 0.911
2.349AlaPhe: 2.349 ± 0.561
4.164AlaGly: 4.164 ± 0.829
0.854AlaHis: 0.854 ± 0.323
4.057AlaIle: 4.057 ± 0.528
5.658AlaLys: 5.658 ± 0.996
6.939AlaLeu: 6.939 ± 0.819
2.882AlaMet: 2.882 ± 0.497
2.562AlaAsn: 2.562 ± 0.533
1.601AlaPro: 1.601 ± 0.53
2.669AlaGln: 2.669 ± 0.687
2.349AlaArg: 2.349 ± 0.549
3.523AlaSer: 3.523 ± 0.661
4.164AlaThr: 4.164 ± 0.733
3.523AlaVal: 3.523 ± 0.752
1.922AlaTrp: 1.922 ± 0.708
3.523AlaTyr: 3.523 ± 0.547
0.0AlaXaa: 0.0 ± 0.0
Cys
0.534CysAla: 0.534 ± 0.303
0.107CysCys: 0.107 ± 0.108
0.427CysAsp: 0.427 ± 0.215
0.427CysGlu: 0.427 ± 0.296
0.107CysPhe: 0.107 ± 0.115
0.534CysGly: 0.534 ± 0.299
0.32CysHis: 0.32 ± 0.233
0.214CysIle: 0.214 ± 0.129
0.427CysLys: 0.427 ± 0.245
0.214CysLeu: 0.214 ± 0.176
0.214CysMet: 0.214 ± 0.156
0.32CysAsn: 0.32 ± 0.197
0.214CysPro: 0.214 ± 0.164
0.214CysGln: 0.214 ± 0.139
0.32CysArg: 0.32 ± 0.22
0.32CysSer: 0.32 ± 0.165
0.427CysThr: 0.427 ± 0.22
0.32CysVal: 0.32 ± 0.2
0.0CysTrp: 0.0 ± 0.0
0.641CysTyr: 0.641 ± 0.246
0.0CysXaa: 0.0 ± 0.0
Asp
4.057AspAla: 4.057 ± 0.606
0.32AspCys: 0.32 ± 0.223
3.096AspAsp: 3.096 ± 0.592
4.911AspGlu: 4.911 ± 0.794
2.242AspPhe: 2.242 ± 0.451
3.63AspGly: 3.63 ± 0.559
0.427AspHis: 0.427 ± 0.364
4.484AspIle: 4.484 ± 0.797
4.057AspLys: 4.057 ± 0.569
4.804AspLeu: 4.804 ± 0.904
1.922AspMet: 1.922 ± 0.456
3.096AspAsn: 3.096 ± 0.556
2.562AspPro: 2.562 ± 0.415
1.601AspGln: 1.601 ± 0.351
1.815AspArg: 1.815 ± 0.529
3.203AspSer: 3.203 ± 0.549
3.416AspThr: 3.416 ± 0.506
5.018AspVal: 5.018 ± 0.569
1.068AspTrp: 1.068 ± 0.393
2.135AspTyr: 2.135 ± 0.418
0.0AspXaa: 0.0 ± 0.0
Glu
5.765GluAla: 5.765 ± 1.167
0.854GluCys: 0.854 ± 0.299
5.124GluAsp: 5.124 ± 0.735
6.832GluGlu: 6.832 ± 1.161
2.989GluPhe: 2.989 ± 0.538
3.203GluGly: 3.203 ± 0.458
1.281GluHis: 1.281 ± 0.398
5.658GluIle: 5.658 ± 0.754
7.58GluLys: 7.58 ± 0.948
7.26GluLeu: 7.26 ± 1.105
3.096GluMet: 3.096 ± 0.624
5.124GluAsn: 5.124 ± 0.799
1.815GluPro: 1.815 ± 0.48
3.843GluGln: 3.843 ± 0.907
3.63GluArg: 3.63 ± 0.669
3.309GluSer: 3.309 ± 0.573
3.416GluThr: 3.416 ± 0.569
3.63GluVal: 3.63 ± 0.882
0.961GluTrp: 0.961 ± 0.353
2.349GluTyr: 2.349 ± 0.485
0.0GluXaa: 0.0 ± 0.0
Phe
2.242PheAla: 2.242 ± 0.436
0.641PheCys: 0.641 ± 0.27
2.028PheAsp: 2.028 ± 0.464
2.455PheGlu: 2.455 ± 0.507
1.495PhePhe: 1.495 ± 0.604
2.882PheGly: 2.882 ± 0.726
0.427PheHis: 0.427 ± 0.27
2.882PheIle: 2.882 ± 0.496
3.63PheLys: 3.63 ± 0.336
2.669PheLeu: 2.669 ± 0.548
0.747PheMet: 0.747 ± 0.333
2.028PheAsn: 2.028 ± 0.474
1.601PhePro: 1.601 ± 0.375
0.961PheGln: 0.961 ± 0.439
1.388PheArg: 1.388 ± 0.346
3.523PheSer: 3.523 ± 0.662
2.669PheThr: 2.669 ± 0.517
2.989PheVal: 2.989 ± 0.852
0.427PheTrp: 0.427 ± 0.238
2.242PheTyr: 2.242 ± 0.596
0.0PheXaa: 0.0 ± 0.0
Gly
4.27GlyAla: 4.27 ± 0.775
0.534GlyCys: 0.534 ± 0.278
2.882GlyAsp: 2.882 ± 0.467
5.445GlyGlu: 5.445 ± 0.636
3.309GlyPhe: 3.309 ± 0.498
3.096GlyGly: 3.096 ± 0.742
1.068GlyHis: 1.068 ± 0.35
5.338GlyIle: 5.338 ± 0.739
5.124GlyLys: 5.124 ± 0.936
5.338GlyLeu: 5.338 ± 0.719
1.922GlyMet: 1.922 ± 0.48
3.523GlyAsn: 3.523 ± 0.673
0.641GlyPro: 0.641 ± 0.246
1.815GlyGln: 1.815 ± 0.535
3.096GlyArg: 3.096 ± 0.658
3.523GlySer: 3.523 ± 0.58
3.95GlyThr: 3.95 ± 1.03
4.911GlyVal: 4.911 ± 0.679
0.747GlyTrp: 0.747 ± 0.232
2.776GlyTyr: 2.776 ± 0.716
0.0GlyXaa: 0.0 ± 0.0
His
0.534HisAla: 0.534 ± 0.239
0.107HisCys: 0.107 ± 0.118
0.854HisAsp: 0.854 ± 0.303
0.641HisGlu: 0.641 ± 0.282
0.427HisPhe: 0.427 ± 0.177
1.174HisGly: 1.174 ± 0.414
0.214HisHis: 0.214 ± 0.216
1.174HisIle: 1.174 ± 0.323
1.495HisLys: 1.495 ± 0.384
1.281HisLeu: 1.281 ± 0.428
0.214HisMet: 0.214 ± 0.159
0.961HisAsn: 0.961 ± 0.29
0.32HisPro: 0.32 ± 0.193
0.214HisGln: 0.214 ± 0.136
0.854HisArg: 0.854 ± 0.283
0.747HisSer: 0.747 ± 0.209
0.534HisThr: 0.534 ± 0.224
1.922HisVal: 1.922 ± 0.527
0.214HisTrp: 0.214 ± 0.158
0.641HisTyr: 0.641 ± 0.327
0.0HisXaa: 0.0 ± 0.0
Ile
5.018IleAla: 5.018 ± 0.529
0.107IleCys: 0.107 ± 0.115
3.63IleAsp: 3.63 ± 0.626
7.046IleGlu: 7.046 ± 1.161
2.349IlePhe: 2.349 ± 0.444
4.911IleGly: 4.911 ± 0.587
1.068IleHis: 1.068 ± 0.435
4.27IleIle: 4.27 ± 0.704
5.338IleLys: 5.338 ± 0.737
5.018IleLeu: 5.018 ± 0.968
2.562IleMet: 2.562 ± 0.611
3.416IleAsn: 3.416 ± 0.404
2.349IlePro: 2.349 ± 0.435
3.523IleGln: 3.523 ± 0.672
2.989IleArg: 2.989 ± 0.648
4.377IleSer: 4.377 ± 0.481
5.445IleThr: 5.445 ± 0.907
4.484IleVal: 4.484 ± 0.566
0.747IleTrp: 0.747 ± 0.31
3.096IleTyr: 3.096 ± 0.662
0.0IleXaa: 0.0 ± 0.0
Lys
5.658LysAla: 5.658 ± 0.74
0.107LysCys: 0.107 ± 0.115
4.591LysAsp: 4.591 ± 0.671
7.473LysGlu: 7.473 ± 1.463
2.669LysPhe: 2.669 ± 0.509
5.765LysGly: 5.765 ± 0.815
1.601LysHis: 1.601 ± 0.437
6.405LysIle: 6.405 ± 0.903
10.996LysLys: 10.996 ± 1.429
6.512LysLeu: 6.512 ± 1.039
3.416LysMet: 3.416 ± 0.486
6.192LysAsn: 6.192 ± 0.909
1.601LysPro: 1.601 ± 0.456
4.591LysGln: 4.591 ± 0.729
3.843LysArg: 3.843 ± 0.461
4.804LysSer: 4.804 ± 0.683
5.551LysThr: 5.551 ± 0.919
5.551LysVal: 5.551 ± 0.89
1.281LysTrp: 1.281 ± 0.509
2.562LysTyr: 2.562 ± 0.516
0.0LysXaa: 0.0 ± 0.0
Leu
6.512LeuAla: 6.512 ± 1.15
0.961LeuCys: 0.961 ± 0.378
4.911LeuAsp: 4.911 ± 0.692
6.512LeuGlu: 6.512 ± 1.207
3.737LeuPhe: 3.737 ± 0.559
7.26LeuGly: 7.26 ± 0.894
1.068LeuHis: 1.068 ± 0.504
4.484LeuIle: 4.484 ± 0.741
6.192LeuLys: 6.192 ± 1.055
7.26LeuLeu: 7.26 ± 1.358
2.242LeuMet: 2.242 ± 0.535
5.018LeuAsn: 5.018 ± 0.706
1.601LeuPro: 1.601 ± 0.362
2.669LeuGln: 2.669 ± 0.775
4.484LeuArg: 4.484 ± 0.83
6.299LeuSer: 6.299 ± 1.044
4.057LeuThr: 4.057 ± 0.578
3.523LeuVal: 3.523 ± 0.518
1.068LeuTrp: 1.068 ± 0.41
1.922LeuTyr: 1.922 ± 0.533
0.0LeuXaa: 0.0 ± 0.0
Met
2.028MetAla: 2.028 ± 0.447
0.107MetCys: 0.107 ± 0.109
2.455MetAsp: 2.455 ± 0.511
1.815MetGlu: 1.815 ± 0.447
0.641MetPhe: 0.641 ± 0.251
1.601MetGly: 1.601 ± 0.393
0.32MetHis: 0.32 ± 0.22
2.349MetIle: 2.349 ± 0.58
3.63MetLys: 3.63 ± 0.562
2.455MetLeu: 2.455 ± 0.523
0.747MetMet: 0.747 ± 0.279
1.815MetAsn: 1.815 ± 0.344
0.747MetPro: 0.747 ± 0.322
0.747MetGln: 0.747 ± 0.286
1.708MetArg: 1.708 ± 0.385
1.922MetSer: 1.922 ± 0.433
1.281MetThr: 1.281 ± 0.401
1.601MetVal: 1.601 ± 0.368
0.107MetTrp: 0.107 ± 0.111
1.388MetTyr: 1.388 ± 0.605
0.0MetXaa: 0.0 ± 0.0
Asn
3.63AsnAla: 3.63 ± 0.718
0.107AsnCys: 0.107 ± 0.109
3.63AsnAsp: 3.63 ± 0.62
4.164AsnGlu: 4.164 ± 0.693
1.601AsnPhe: 1.601 ± 0.46
5.231AsnGly: 5.231 ± 1.015
0.641AsnHis: 0.641 ± 0.234
4.804AsnIle: 4.804 ± 0.723
5.018AsnLys: 5.018 ± 0.925
4.804AsnLeu: 4.804 ± 0.599
1.708AsnMet: 1.708 ± 0.377
2.562AsnAsn: 2.562 ± 0.522
2.028AsnPro: 2.028 ± 0.493
2.135AsnGln: 2.135 ± 0.518
1.601AsnArg: 1.601 ± 0.523
2.776AsnSer: 2.776 ± 0.639
3.63AsnThr: 3.63 ± 0.66
3.416AsnVal: 3.416 ± 0.57
0.747AsnTrp: 0.747 ± 0.336
2.349AsnTyr: 2.349 ± 0.479
0.0AsnXaa: 0.0 ± 0.0
Pro
1.068ProAla: 1.068 ± 0.353
0.107ProCys: 0.107 ± 0.107
2.028ProAsp: 2.028 ± 0.567
1.281ProGlu: 1.281 ± 0.344
1.388ProPhe: 1.388 ± 0.533
1.281ProGly: 1.281 ± 0.424
0.32ProHis: 0.32 ± 0.152
2.776ProIle: 2.776 ± 0.546
3.096ProLys: 3.096 ± 0.691
2.882ProLeu: 2.882 ± 0.592
0.32ProMet: 0.32 ± 0.198
1.708ProAsn: 1.708 ± 0.556
0.641ProPro: 0.641 ± 0.254
1.174ProGln: 1.174 ± 0.381
0.961ProArg: 0.961 ± 0.395
1.922ProSer: 1.922 ± 0.49
2.135ProThr: 2.135 ± 0.489
2.349ProVal: 2.349 ± 0.555
0.214ProTrp: 0.214 ± 0.162
0.854ProTyr: 0.854 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
3.096GlnAla: 3.096 ± 0.716
0.214GlnCys: 0.214 ± 0.154
1.495GlnAsp: 1.495 ± 0.307
3.203GlnGlu: 3.203 ± 0.807
2.135GlnPhe: 2.135 ± 0.496
2.242GlnGly: 2.242 ± 0.453
0.534GlnHis: 0.534 ± 0.224
3.096GlnIle: 3.096 ± 0.53
3.737GlnLys: 3.737 ± 0.629
3.416GlnLeu: 3.416 ± 0.571
0.641GlnMet: 0.641 ± 0.236
2.028GlnAsn: 2.028 ± 0.526
1.601GlnPro: 1.601 ± 0.439
2.242GlnGln: 2.242 ± 0.59
1.281GlnArg: 1.281 ± 0.358
2.135GlnSer: 2.135 ± 0.534
2.349GlnThr: 2.349 ± 0.523
1.922GlnVal: 1.922 ± 0.491
0.961GlnTrp: 0.961 ± 0.363
1.495GlnTyr: 1.495 ± 0.375
0.0GlnXaa: 0.0 ± 0.0
Arg
2.242ArgAla: 2.242 ± 0.559
0.214ArgCys: 0.214 ± 0.139
2.349ArgAsp: 2.349 ± 0.444
3.096ArgGlu: 3.096 ± 0.575
2.242ArgPhe: 2.242 ± 0.529
2.242ArgGly: 2.242 ± 0.599
0.747ArgHis: 0.747 ± 0.242
2.989ArgIle: 2.989 ± 0.809
4.164ArgLys: 4.164 ± 0.76
2.669ArgLeu: 2.669 ± 0.789
1.174ArgMet: 1.174 ± 0.288
2.455ArgAsn: 2.455 ± 0.48
1.495ArgPro: 1.495 ± 0.371
1.388ArgGln: 1.388 ± 0.338
1.815ArgArg: 1.815 ± 0.581
2.669ArgSer: 2.669 ± 0.549
2.562ArgThr: 2.562 ± 0.506
2.989ArgVal: 2.989 ± 0.562
0.747ArgTrp: 0.747 ± 0.294
1.281ArgTyr: 1.281 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
3.843SerAla: 3.843 ± 0.567
0.107SerCys: 0.107 ± 0.134
3.416SerAsp: 3.416 ± 0.582
4.27SerGlu: 4.27 ± 0.91
3.309SerPhe: 3.309 ± 0.494
4.164SerGly: 4.164 ± 0.732
0.747SerHis: 0.747 ± 0.313
4.377SerIle: 4.377 ± 0.692
4.27SerLys: 4.27 ± 0.714
4.911SerLeu: 4.911 ± 0.61
1.708SerMet: 1.708 ± 0.565
3.096SerAsn: 3.096 ± 0.936
1.708SerPro: 1.708 ± 0.462
2.135SerGln: 2.135 ± 0.572
2.349SerArg: 2.349 ± 0.549
4.27SerSer: 4.27 ± 0.874
4.27SerThr: 4.27 ± 0.799
3.309SerVal: 3.309 ± 0.404
0.854SerTrp: 0.854 ± 0.273
2.349SerTyr: 2.349 ± 0.393
0.0SerXaa: 0.0 ± 0.0
Thr
4.484ThrAla: 4.484 ± 0.821
0.427ThrCys: 0.427 ± 0.198
4.27ThrAsp: 4.27 ± 0.579
3.63ThrGlu: 3.63 ± 0.776
1.815ThrPhe: 1.815 ± 0.397
4.591ThrGly: 4.591 ± 0.713
1.281ThrHis: 1.281 ± 0.321
3.309ThrIle: 3.309 ± 0.611
5.445ThrLys: 5.445 ± 0.858
5.231ThrLeu: 5.231 ± 0.839
1.068ThrMet: 1.068 ± 0.387
2.776ThrAsn: 2.776 ± 0.709
2.455ThrPro: 2.455 ± 0.566
2.455ThrGln: 2.455 ± 0.515
1.601ThrArg: 1.601 ± 0.413
3.63ThrSer: 3.63 ± 0.613
2.455ThrThr: 2.455 ± 0.434
3.95ThrVal: 3.95 ± 0.836
0.961ThrTrp: 0.961 ± 0.356
2.562ThrTyr: 2.562 ± 0.659
0.0ThrXaa: 0.0 ± 0.0
Val
3.95ValAla: 3.95 ± 0.828
0.641ValCys: 0.641 ± 0.265
3.416ValAsp: 3.416 ± 0.538
4.804ValGlu: 4.804 ± 0.88
2.669ValPhe: 2.669 ± 0.558
2.669ValGly: 2.669 ± 0.638
0.747ValHis: 0.747 ± 0.372
4.697ValIle: 4.697 ± 0.764
6.192ValLys: 6.192 ± 0.774
3.63ValLeu: 3.63 ± 0.592
1.495ValMet: 1.495 ± 0.332
4.591ValAsn: 4.591 ± 0.759
2.028ValPro: 2.028 ± 0.452
3.737ValGln: 3.737 ± 0.651
2.776ValArg: 2.776 ± 0.552
3.843ValSer: 3.843 ± 0.589
3.737ValThr: 3.737 ± 0.642
3.096ValVal: 3.096 ± 0.458
0.641ValTrp: 0.641 ± 0.225
2.882ValTyr: 2.882 ± 0.534
0.0ValXaa: 0.0 ± 0.0
Trp
0.534TrpAla: 0.534 ± 0.25
0.0TrpCys: 0.0 ± 0.0
0.854TrpAsp: 0.854 ± 0.318
1.601TrpGlu: 1.601 ± 0.407
0.641TrpPhe: 0.641 ± 0.231
0.641TrpGly: 0.641 ± 0.286
0.0TrpHis: 0.0 ± 0.0
1.068TrpIle: 1.068 ± 0.304
1.495TrpLys: 1.495 ± 0.353
1.281TrpLeu: 1.281 ± 0.406
0.32TrpMet: 0.32 ± 0.204
1.281TrpAsn: 1.281 ± 0.363
0.0TrpPro: 0.0 ± 0.0
0.534TrpGln: 0.534 ± 0.202
0.641TrpArg: 0.641 ± 0.216
1.281TrpSer: 1.281 ± 0.56
0.641TrpThr: 0.641 ± 0.31
0.641TrpVal: 0.641 ± 0.254
0.0TrpTrp: 0.0 ± 0.0
0.534TrpTyr: 0.534 ± 0.247
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.242TyrAla: 2.242 ± 0.453
0.427TyrCys: 0.427 ± 0.302
2.669TyrAsp: 2.669 ± 0.531
3.203TyrGlu: 3.203 ± 0.64
1.815TyrPhe: 1.815 ± 0.352
2.028TyrGly: 2.028 ± 0.385
0.854TyrHis: 0.854 ± 0.283
3.309TyrIle: 3.309 ± 0.388
3.63TyrLys: 3.63 ± 0.62
3.203TyrLeu: 3.203 ± 0.894
0.961TyrMet: 0.961 ± 0.312
2.028TyrAsn: 2.028 ± 0.497
1.495TyrPro: 1.495 ± 0.456
1.174TyrGln: 1.174 ± 0.306
2.028TyrArg: 2.028 ± 0.464
1.388TyrSer: 1.388 ± 0.385
1.708TyrThr: 1.708 ± 0.383
3.096TyrVal: 3.096 ± 0.59
0.32TyrTrp: 0.32 ± 0.199
1.174TyrTyr: 1.174 ± 0.378
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (9368 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski