Nonomuraea solani

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6D4K4|A0A1H6D4K4_9ACTN Uncharacterized protein OS=Nonomuraea solani OX=1144553 GN=SAMN05444920_104720 PE=4 SV=1
MM1 pKa = 7.17NPIVPTAKK9 pKa = 10.18RR10 pKa = 11.84GIGAAALAAGMVLASPITAGAAAAPPATYY39 pKa = 10.61ASVVGDD45 pKa = 3.68TLNVDD50 pKa = 3.92GNYY53 pKa = 10.01EE54 pKa = 4.41SNHH57 pKa = 4.6ITITPAAYY65 pKa = 9.91GRR67 pKa = 11.84VRR69 pKa = 11.84IADD72 pKa = 3.84ANNPTLPGGDD82 pKa = 4.48CIDD85 pKa = 3.65ITDD88 pKa = 4.32HH89 pKa = 6.57EE90 pKa = 5.07VEE92 pKa = 5.0CGLSGTPTGLDD103 pKa = 3.19VDD105 pKa = 4.05GHH107 pKa = 6.32EE108 pKa = 4.85GADD111 pKa = 3.69TIINLLPTSNKK122 pKa = 7.47TQARR126 pKa = 11.84LHH128 pKa = 6.36GGSGNDD134 pKa = 3.51VIYY137 pKa = 10.44GGPGVQWLNGGLSPDD152 pKa = 4.5DD153 pKa = 3.74LAANQGRR160 pKa = 11.84PDD162 pKa = 3.52TGNDD166 pKa = 3.25KK167 pKa = 11.24LFGGCAQEE175 pKa = 4.76CADD178 pKa = 5.29SGDD181 pKa = 4.04LLEE184 pKa = 5.95GSDD187 pKa = 4.59GNDD190 pKa = 3.36GLTGGPGNDD199 pKa = 4.29DD200 pKa = 3.43LRR202 pKa = 11.84GGIGVDD208 pKa = 3.85TYY210 pKa = 11.7AGGSGRR216 pKa = 11.84DD217 pKa = 3.45VVSYY221 pKa = 10.78ADD223 pKa = 3.12KK224 pKa = 10.76GVRR227 pKa = 11.84VQASLNNVADD237 pKa = 4.36DD238 pKa = 5.19GIAGEE243 pKa = 4.84QEE245 pKa = 3.99NLPNDD250 pKa = 3.36VEE252 pKa = 4.81DD253 pKa = 3.86IYY255 pKa = 11.33GGRR258 pKa = 11.84SADD261 pKa = 3.41VLTGNDD267 pKa = 4.18ADD269 pKa = 3.83NTLNGGPGGGDD280 pKa = 2.97ALYY283 pKa = 11.03GNGGSDD289 pKa = 3.19TLYY292 pKa = 10.92GGSGVDD298 pKa = 4.68KK299 pKa = 11.01LYY301 pKa = 11.4GDD303 pKa = 4.44YY304 pKa = 10.51KK305 pKa = 10.86DD306 pKa = 4.47SAMTYY311 pKa = 10.51GNDD314 pKa = 3.46YY315 pKa = 10.79LYY317 pKa = 11.31GGADD321 pKa = 3.47GDD323 pKa = 4.46LLVGGWGIDD332 pKa = 3.17HH333 pKa = 6.31SRR335 pKa = 11.84EE336 pKa = 3.61YY337 pKa = 10.91DD338 pKa = 3.4YY339 pKa = 10.56DD340 pKa = 3.86TGTDD344 pKa = 3.09TCQSVEE350 pKa = 4.32SEE352 pKa = 4.2DD353 pKa = 3.72HH354 pKa = 6.97DD355 pKa = 3.67KK356 pKa = 11.73CEE358 pKa = 5.72AVLPWPP364 pKa = 4.3

Molecular weight: 36.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6EZP3|A0A1H6EZP3_9ACTN Transport permease protein OS=Nonomuraea solani OX=1144553 GN=SAMN05444920_13610 PE=3 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84GRR4 pKa = 11.84ARR6 pKa = 11.84LRR8 pKa = 11.84ALLRR12 pKa = 11.84VLGRR16 pKa = 11.84MPSPARR22 pKa = 11.84SGPRR26 pKa = 11.84RR27 pKa = 11.84SGCLGPYY34 pKa = 9.14TSGTRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84TPLSLGRR50 pKa = 11.84LRR52 pKa = 11.84SLRR55 pKa = 11.84RR56 pKa = 11.84VTPRR60 pKa = 11.84ARR62 pKa = 11.84PRR64 pKa = 11.84CPQRR68 pKa = 11.84LGRR71 pKa = 11.84PAPRR75 pKa = 11.84LTRR78 pKa = 11.84RR79 pKa = 11.84APAPAAPRR87 pKa = 11.84PNQAPHH93 pKa = 6.9PPNRR97 pKa = 11.84PPPAPLRR104 pKa = 11.84PPSRR108 pKa = 11.84ALHH111 pKa = 5.84LPSRR115 pKa = 11.84APPARR120 pKa = 11.84LPLRR124 pKa = 11.84RR125 pKa = 11.84APLVARR131 pKa = 11.84RR132 pKa = 11.84PPSLVLPPGLRR143 pKa = 11.84PPNRR147 pKa = 11.84ALRR150 pKa = 11.84LPSRR154 pKa = 11.84APVVVPHH161 pKa = 6.88PSRR164 pKa = 11.84APLMAPCPRR173 pKa = 11.84QLVLMAPRR181 pKa = 11.84PRR183 pKa = 11.84RR184 pKa = 11.84APLMVPRR191 pKa = 11.84PRR193 pKa = 11.84RR194 pKa = 11.84PVLRR198 pKa = 11.84GRR200 pKa = 11.84PRR202 pKa = 11.84RR203 pKa = 11.84APALPVGRR211 pKa = 11.84GRR213 pKa = 11.84LRR215 pKa = 11.84RR216 pKa = 11.84PPRR219 pKa = 11.84TSPRR223 pKa = 11.84PSPASAPSRR232 pKa = 11.84PAARR236 pKa = 11.84NRR238 pKa = 11.84PVGLPRR244 pKa = 11.84QVRR247 pKa = 11.84ARR249 pKa = 11.84ARR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84ACDD256 pKa = 3.41RR257 pKa = 11.84SPPAALRR264 pKa = 11.84ARR266 pKa = 11.84PGPNRR271 pKa = 11.84RR272 pKa = 11.84ATLRR276 pKa = 11.84PTPRR280 pKa = 11.84RR281 pKa = 11.84RR282 pKa = 11.84ARR284 pKa = 11.84PSQAA288 pKa = 3.03

Molecular weight: 31.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12105

0

12105

3978721

27

4145

328.7

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.182 ± 0.032

0.765 ± 0.006

5.781 ± 0.018

5.567 ± 0.022

2.877 ± 0.014

9.371 ± 0.023

2.241 ± 0.01

3.62 ± 0.013

2.1 ± 0.017

10.685 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.9 ± 0.009

1.874 ± 0.014

6.001 ± 0.018

2.715 ± 0.012

8.083 ± 0.029

4.976 ± 0.017

6.018 ± 0.023

8.426 ± 0.021

1.614 ± 0.01

2.204 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski