Exilibacterium tricleocarpae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Cellvibrionaceae; Exilibacterium

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A545T5W8|A0A545T5W8_9GAMM HAD-IA family hydrolase OS=Exilibacterium tricleocarpae OX=2591008 GN=FKG94_18135 PE=4 SV=1
MM1 pKa = 7.33LVKK4 pKa = 10.5LRR6 pKa = 11.84QIKK9 pKa = 7.05QHH11 pKa = 6.27KK12 pKa = 9.57NRR14 pKa = 11.84LLKK17 pKa = 10.21RR18 pKa = 11.84WAVLSMLVLLAACDD32 pKa = 4.12DD33 pKa = 4.12NGPAEE38 pKa = 4.4NQPPQAPPLALTTAEE53 pKa = 4.56DD54 pKa = 4.18TPLSITLPGTDD65 pKa = 2.77SDD67 pKa = 4.89GFITYY72 pKa = 9.78HH73 pKa = 5.91SVVTAPVHH81 pKa = 5.06GTLEE85 pKa = 4.4GVAPDD90 pKa = 4.57LLYY93 pKa = 10.53IPDD96 pKa = 3.93ADD98 pKa = 4.14FFGSDD103 pKa = 3.1SFVYY107 pKa = 10.54RR108 pKa = 11.84LTDD111 pKa = 3.13NAGAVSSATVSIEE124 pKa = 3.71VTAVNDD130 pKa = 3.54APVAVDD136 pKa = 4.2DD137 pKa = 4.69SYY139 pKa = 11.99TVAGYY144 pKa = 8.83TSTTIAAPGVLSNDD158 pKa = 3.1SDD160 pKa = 3.72IDD162 pKa = 3.92SASTDD167 pKa = 3.53LTTAAATLDD176 pKa = 3.6TRR178 pKa = 11.84EE179 pKa = 4.3GGTVTMAVDD188 pKa = 3.97GGFTYY193 pKa = 9.99TPPSTVGLTEE203 pKa = 5.01DD204 pKa = 3.3SFEE207 pKa = 4.21YY208 pKa = 10.18TVSDD212 pKa = 3.58NHH214 pKa = 5.95PTAPQSATARR224 pKa = 11.84VTLVLTDD231 pKa = 3.99TGNQPPLARR240 pKa = 11.84DD241 pKa = 3.32LALTTSEE248 pKa = 4.33DD249 pKa = 3.56TSLSILLQGEE259 pKa = 4.24DD260 pKa = 3.33ADD262 pKa = 4.39GVVTAYY268 pKa = 10.77SVITPPTRR276 pKa = 11.84GRR278 pKa = 11.84LSGAAPAMTYY288 pKa = 9.75TPAANYY294 pKa = 9.17FGSDD298 pKa = 3.1SFVYY302 pKa = 10.24GVTDD306 pKa = 3.37NAGATSTATVTITITPVNDD325 pKa = 3.33APVALDD331 pKa = 4.05DD332 pKa = 4.22SFTVARR338 pKa = 11.84NKK340 pKa = 8.68PTVVAAPGVLANDD353 pKa = 3.93SDD355 pKa = 4.32IEE357 pKa = 4.28SAPASLTVTAQTITTARR374 pKa = 11.84GGSVNLDD381 pKa = 3.08SRR383 pKa = 11.84GGFIYY388 pKa = 10.39SPPLTAGVAEE398 pKa = 5.49DD399 pKa = 3.61SFQYY403 pKa = 10.21TLSDD407 pKa = 3.65NDD409 pKa = 3.44PTGARR414 pKa = 11.84FSTGRR419 pKa = 11.84VTLILTGAEE428 pKa = 4.14NQRR431 pKa = 11.84PTAVDD436 pKa = 3.88LNLATPEE443 pKa = 4.03DD444 pKa = 3.92QPLGVTLRR452 pKa = 11.84GEE454 pKa = 4.61DD455 pKa = 3.57GDD457 pKa = 4.43GVIVSYY463 pKa = 11.06AVTVPPTRR471 pKa = 11.84GRR473 pKa = 11.84LTGSAPNLTYY483 pKa = 10.32TPQPDD488 pKa = 3.82YY489 pKa = 11.26FGDD492 pKa = 3.54DD493 pKa = 3.13SFTYY497 pKa = 10.13RR498 pKa = 11.84VTDD501 pKa = 3.77DD502 pKa = 3.44VGATATGTVRR512 pKa = 11.84ITVSTVNDD520 pKa = 3.97APTAAADD527 pKa = 3.47SFNAVRR533 pKa = 11.84NALTTVAAPGVLANDD548 pKa = 3.73SDD550 pKa = 4.24IEE552 pKa = 4.29SAAATLTVTATTLATAQDD570 pKa = 3.96GVVTLNADD578 pKa = 3.36GGFTYY583 pKa = 9.84TPPPTAGLTTDD594 pKa = 3.59SFQYY598 pKa = 10.04TLNDD602 pKa = 3.63NDD604 pKa = 4.33PVDD607 pKa = 4.14PLSATGEE614 pKa = 4.14VTLILTGAEE623 pKa = 4.1NLPPSATDD631 pKa = 3.43LTLTTAEE638 pKa = 4.98DD639 pKa = 3.88SPLSITLQGADD650 pKa = 3.29SDD652 pKa = 4.69GAVATYY658 pKa = 9.36TVTTPPQHH666 pKa = 6.15GRR668 pKa = 11.84LTGVAPDD675 pKa = 3.66LTYY678 pKa = 10.86TPDD681 pKa = 3.45ADD683 pKa = 4.04YY684 pKa = 10.79FGSDD688 pKa = 3.02SFVYY692 pKa = 10.18EE693 pKa = 4.1VTDD696 pKa = 3.89SIGAIASATVTLTVNAVNDD715 pKa = 4.21APTAAADD722 pKa = 3.72SFNAARR728 pKa = 11.84NALTTVAAPGVLANDD743 pKa = 3.91SDD745 pKa = 4.47IEE747 pKa = 4.36STPAALTVTAVTLATAQGGAVTLNADD773 pKa = 3.52GGFTYY778 pKa = 9.84TPPPTAGLTTDD789 pKa = 3.59SFQYY793 pKa = 10.04TLNDD797 pKa = 3.63NDD799 pKa = 4.33PVDD802 pKa = 4.14PLSATGEE809 pKa = 4.14VTLILTGAEE818 pKa = 4.1NLPPSATDD826 pKa = 3.43LTLTTAEE833 pKa = 4.98DD834 pKa = 3.88SPLSITLQGTDD845 pKa = 2.5SDD847 pKa = 4.62GAVVAYY853 pKa = 8.33TVTTPPQHH861 pKa = 5.75GQLAGVAPNLIYY873 pKa = 10.54TPAADD878 pKa = 3.84YY879 pKa = 10.45FGSDD883 pKa = 3.03SFVYY887 pKa = 10.18EE888 pKa = 4.1VTDD891 pKa = 3.89SIGAIASATVTLTINAVNDD910 pKa = 3.84APTAAADD917 pKa = 3.72SFNAARR923 pKa = 11.84NTLTTVAAPGVLANDD938 pKa = 3.76SDD940 pKa = 4.25IEE942 pKa = 4.4SVPAEE947 pKa = 4.21LTVTAATLATAQGGAVTLNADD968 pKa = 3.52GGFTYY973 pKa = 9.81TPPVTTGLSTDD984 pKa = 3.23SFQYY988 pKa = 10.07TLNDD992 pKa = 3.63NDD994 pKa = 4.33PVDD997 pKa = 4.14PLSATGEE1004 pKa = 4.14VTLILTGAEE1013 pKa = 4.1NLPPSATDD1021 pKa = 3.43LTLTTAEE1028 pKa = 4.98DD1029 pKa = 3.88SPLSITLQGADD1040 pKa = 2.91SDD1042 pKa = 4.64GAVVAYY1048 pKa = 8.33TVTTPPQHH1056 pKa = 5.75GQLAGVAPNLTYY1068 pKa = 10.53TPAADD1073 pKa = 3.75YY1074 pKa = 10.39FGSDD1078 pKa = 2.98SFIYY1082 pKa = 10.4AVTDD1086 pKa = 3.47NLGATASATVALTISAVNDD1105 pKa = 3.27APTAVDD1111 pKa = 5.37DD1112 pKa = 5.77DD1113 pKa = 4.32FTAQGNIITLIAAPGVLANDD1133 pKa = 3.98SDD1135 pKa = 4.54VEE1137 pKa = 4.39SADD1140 pKa = 3.78ADD1142 pKa = 3.8LTATAEE1148 pKa = 4.52TIATDD1153 pKa = 3.13QGGSVTINTNGSLLYY1168 pKa = 9.59TPPPAAGVTADD1179 pKa = 3.6SFHH1182 pKa = 6.2YY1183 pKa = 9.41TLNDD1187 pKa = 3.39NDD1189 pKa = 4.26PVDD1192 pKa = 4.08PRR1194 pKa = 11.84TSTGTVTIALTTYY1207 pKa = 10.25TEE1209 pKa = 4.24GLSVQGLPAASGDD1222 pKa = 3.82SATARR1227 pKa = 11.84LYY1229 pKa = 11.27LSGTAEE1235 pKa = 4.69SMLSYY1240 pKa = 10.17QWSADD1245 pKa = 3.48GSWVIDD1251 pKa = 3.4SGQGSEE1257 pKa = 4.91VITLTAPAAAGAANVSVVVEE1277 pKa = 4.25DD1278 pKa = 4.45DD1279 pKa = 3.38SGNRR1283 pKa = 11.84AVASLPLSKK1292 pKa = 10.6AGEE1295 pKa = 4.1TAPVLEE1301 pKa = 4.27SLYY1304 pKa = 11.13LEE1306 pKa = 5.09LPPASAPVEE1315 pKa = 4.21LAVTAYY1321 pKa = 10.65DD1322 pKa = 3.96PDD1324 pKa = 4.27GLALDD1329 pKa = 4.36YY1330 pKa = 9.53TWKK1333 pKa = 10.74SAGATVASGATAQWQPPLPGRR1354 pKa = 11.84YY1355 pKa = 9.44QIDD1358 pKa = 3.46IEE1360 pKa = 4.34VDD1362 pKa = 3.18NSSLVAGGSLQRR1374 pKa = 11.84HH1375 pKa = 4.85YY1376 pKa = 10.23TGGAPWAFFRR1386 pKa = 11.84GSRR1389 pKa = 11.84QGTGVRR1395 pKa = 11.84IPADD1399 pKa = 3.37TSSNSGALKK1408 pKa = 8.28WQRR1411 pKa = 11.84PFTMANCAVADD1422 pKa = 3.81NFVPGVVQGEE1432 pKa = 4.4DD1433 pKa = 3.06GTLYY1437 pKa = 10.73AGSSTEE1443 pKa = 3.77GKK1445 pKa = 10.35LYY1447 pKa = 10.89AFNPDD1452 pKa = 3.68DD1453 pKa = 5.89GVIAWSFTTVGTRR1466 pKa = 11.84IDD1468 pKa = 3.88ATPVIAEE1475 pKa = 4.59DD1476 pKa = 3.7GSIYY1480 pKa = 10.52VVEE1483 pKa = 4.4SGGTVYY1489 pKa = 10.83AVNADD1494 pKa = 3.79GSEE1497 pKa = 3.86KK1498 pKa = 9.64WSYY1501 pKa = 11.39AIGAPVSGSPAIGADD1516 pKa = 3.16GTVYY1520 pKa = 10.84VGTDD1524 pKa = 2.88NGGASVLLALNPADD1538 pKa = 5.72GLPKK1542 pKa = 9.9WSAPFALAAEE1552 pKa = 4.62TRR1554 pKa = 11.84SSVNIGADD1562 pKa = 3.3GTVYY1566 pKa = 10.88ARR1568 pKa = 11.84DD1569 pKa = 3.37FAGNLFAINPADD1581 pKa = 3.68GTQRR1585 pKa = 11.84WQITLGGGSGSTPVIAADD1603 pKa = 3.37GTLYY1607 pKa = 10.62FGSFSPPRR1615 pKa = 11.84LYY1617 pKa = 10.95AVGPDD1622 pKa = 4.75GSPKK1626 pKa = 9.62WDD1628 pKa = 3.13TAMGNPSLAGIGATPSVGADD1648 pKa = 3.01GTVYY1652 pKa = 10.71VGVWEE1657 pKa = 4.64AGLNGSVYY1665 pKa = 10.68AIDD1668 pKa = 3.85PVGGTVVWRR1677 pKa = 11.84RR1678 pKa = 11.84QLDD1681 pKa = 3.73SFVQSAATAVGADD1694 pKa = 3.06GRR1696 pKa = 11.84VYY1698 pKa = 10.22VVSARR1703 pKa = 11.84GVFYY1707 pKa = 10.92ALDD1710 pKa = 4.48PDD1712 pKa = 3.88NGAVDD1717 pKa = 3.38WSFDD1721 pKa = 2.8IGAATNEE1728 pKa = 4.21EE1729 pKa = 4.74SPSPLAIGTDD1739 pKa = 3.54GSIYY1743 pKa = 10.57VYY1745 pKa = 10.26TCDD1748 pKa = 3.12GVLRR1752 pKa = 11.84AFQQ1755 pKa = 3.44

Molecular weight: 178.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A545U3L0|A0A545U3L0_9GAMM Uncharacterized protein OS=Exilibacterium tricleocarpae OX=2591008 GN=FKG94_05190 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.25RR14 pKa = 11.84NHH16 pKa = 5.6GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.31QLAAA44 pKa = 4.14

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5556

0

5556

2009629

18

12098

361.7

39.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.475 ± 0.053

1.036 ± 0.012

6.002 ± 0.034

5.701 ± 0.029

3.855 ± 0.022

7.86 ± 0.031

2.148 ± 0.017

5.216 ± 0.029

3.544 ± 0.036

10.442 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.025 ± 0.019

3.551 ± 0.026

4.59 ± 0.025

4.198 ± 0.025

6.349 ± 0.028

5.93 ± 0.028

5.557 ± 0.034

7.169 ± 0.027

1.329 ± 0.013

3.025 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski