Amino acid dipepetide frequency for Cowpox virus (strain Brighton Red) (CPV)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.454AlaAla: 2.454 ± 0.214
1.168AlaCys: 1.168 ± 0.147
2.118AlaAsp: 2.118 ± 0.15
1.972AlaGlu: 1.972 ± 0.192
1.679AlaPhe: 1.679 ± 0.162
1.533AlaGly: 1.533 ± 0.168
0.482AlaHis: 0.482 ± 0.093
3.797AlaIle: 3.797 ± 0.234
2.716AlaLys: 2.716 ± 0.203
3.169AlaLeu: 3.169 ± 0.204
1.052AlaMet: 1.052 ± 0.123
2.293AlaAsn: 2.293 ± 0.171
1.008AlaPro: 1.008 ± 0.124
0.672AlaGln: 0.672 ± 0.103
1.65AlaArg: 1.65 ± 0.164
3.271AlaSer: 3.271 ± 0.244
2.512AlaThr: 2.512 ± 0.213
2.848AlaVal: 2.848 ± 0.212
0.19AlaTrp: 0.19 ± 0.048
1.709AlaTyr: 1.709 ± 0.172
0.0AlaXaa: 0.0 ± 0.0
Cys
0.876CysAla: 0.876 ± 0.128
0.613CysCys: 0.613 ± 0.1
1.519CysAsp: 1.519 ± 0.167
1.037CysGlu: 1.037 ± 0.135
0.686CysPhe: 0.686 ± 0.096
1.081CysGly: 1.081 ± 0.115
0.497CysHis: 0.497 ± 0.092
2.147CysIle: 2.147 ± 0.23
1.271CysLys: 1.271 ± 0.152
1.884CysLeu: 1.884 ± 0.17
0.54CysMet: 0.54 ± 0.088
1.387CysAsn: 1.387 ± 0.156
0.774CysPro: 0.774 ± 0.116
0.394CysGln: 0.394 ± 0.084
0.993CysArg: 0.993 ± 0.111
1.767CysSer: 1.767 ± 0.173
1.3CysThr: 1.3 ± 0.178
1.533CysVal: 1.533 ± 0.159
0.204CysTrp: 0.204 ± 0.059
1.256CysTyr: 1.256 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
2.585AspAla: 2.585 ± 0.194
1.212AspCys: 1.212 ± 0.146
5.856AspAsp: 5.856 ± 0.502
4.586AspGlu: 4.586 ± 0.248
2.746AspPhe: 2.746 ± 0.22
2.906AspGly: 2.906 ± 0.186
1.241AspHis: 1.241 ± 0.136
8.324AspIle: 8.324 ± 0.424
5.141AspLys: 5.141 ± 0.262
4.936AspLeu: 4.936 ± 0.252
1.826AspMet: 1.826 ± 0.142
4.717AspAsn: 4.717 ± 0.313
1.694AspPro: 1.694 ± 0.138
1.154AspGln: 1.154 ± 0.13
2.468AspArg: 2.468 ± 0.222
4.527AspSer: 4.527 ± 0.268
3.943AspThr: 3.943 ± 0.277
4.63AspVal: 4.63 ± 0.282
0.482AspTrp: 0.482 ± 0.093
3.856AspTyr: 3.856 ± 0.239
0.0AspXaa: 0.0 ± 0.0
Glu
2.001GluAla: 2.001 ± 0.16
1.183GluCys: 1.183 ± 0.146
3.651GluAsp: 3.651 ± 0.225
3.125GluGlu: 3.125 ± 0.241
2.381GluPhe: 2.381 ± 0.192
1.621GluGly: 1.621 ± 0.153
1.183GluHis: 1.183 ± 0.113
4.761GluIle: 4.761 ± 0.274
3.52GluLys: 3.52 ± 0.251
5.374GluLeu: 5.374 ± 0.392
1.533GluMet: 1.533 ± 0.156
3.461GluAsn: 3.461 ± 0.231
1.811GluPro: 1.811 ± 0.172
1.344GluGln: 1.344 ± 0.133
2.643GluArg: 2.643 ± 0.316
4.031GluSer: 4.031 ± 0.262
3.33GluThr: 3.33 ± 0.244
2.702GluVal: 2.702 ± 0.236
0.526GluTrp: 0.526 ± 0.081
3.856GluTyr: 3.856 ± 0.228
0.0GluXaa: 0.0 ± 0.0
Phe
1.519PheAla: 1.519 ± 0.174
0.803PheCys: 0.803 ± 0.102
3.125PheAsp: 3.125 ± 0.233
2.03PheGlu: 2.03 ± 0.151
1.942PhePhe: 1.942 ± 0.186
1.796PheGly: 1.796 ± 0.155
0.716PheHis: 0.716 ± 0.1
4.571PheIle: 4.571 ± 0.267
3.286PheLys: 3.286 ± 0.208
3.899PheLeu: 3.899 ± 0.331
1.329PheMet: 1.329 ± 0.142
3.257PheAsn: 3.257 ± 0.22
1.314PhePro: 1.314 ± 0.144
0.789PheGln: 0.789 ± 0.093
1.855PheArg: 1.855 ± 0.171
3.593PheSer: 3.593 ± 0.231
2.819PheThr: 2.819 ± 0.229
2.789PheVal: 2.789 ± 0.235
0.336PheTrp: 0.336 ± 0.067
2.264PheTyr: 2.264 ± 0.164
0.0PheXaa: 0.0 ± 0.0
Gly
2.088GlyAla: 2.088 ± 0.175
0.803GlyCys: 0.803 ± 0.091
2.585GlyAsp: 2.585 ± 0.2
2.03GlyGlu: 2.03 ± 0.145
1.723GlyPhe: 1.723 ± 0.147
2.337GlyGly: 2.337 ± 0.216
0.876GlyHis: 0.876 ± 0.11
3.753GlyIle: 3.753 ± 0.234
2.731GlyLys: 2.731 ± 0.239
2.906GlyLeu: 2.906 ± 0.184
0.993GlyMet: 0.993 ± 0.142
2.95GlyAsn: 2.95 ± 0.205
0.92GlyPro: 0.92 ± 0.119
0.613GlyGln: 0.613 ± 0.087
1.869GlyArg: 1.869 ± 0.156
2.819GlySer: 2.819 ± 0.196
2.088GlyThr: 2.088 ± 0.204
2.862GlyVal: 2.862 ± 0.235
0.263GlyTrp: 0.263 ± 0.059
2.366GlyTyr: 2.366 ± 0.178
0.0GlyXaa: 0.0 ± 0.0
His
1.008HisAla: 1.008 ± 0.101
0.584HisCys: 0.584 ± 0.114
1.329HisAsp: 1.329 ± 0.123
0.92HisGlu: 0.92 ± 0.089
0.759HisPhe: 0.759 ± 0.091
1.183HisGly: 1.183 ± 0.143
0.555HisHis: 0.555 ± 0.094
2.307HisIle: 2.307 ± 0.202
1.373HisLys: 1.373 ± 0.131
1.899HisLeu: 1.899 ± 0.171
0.57HisMet: 0.57 ± 0.088
1.256HisAsn: 1.256 ± 0.128
0.832HisPro: 0.832 ± 0.099
0.526HisGln: 0.526 ± 0.085
1.022HisArg: 1.022 ± 0.134
1.431HisSer: 1.431 ± 0.138
1.358HisThr: 1.358 ± 0.152
1.373HisVal: 1.373 ± 0.152
0.19HisTrp: 0.19 ± 0.048
0.949HisTyr: 0.949 ± 0.135
0.0HisXaa: 0.0 ± 0.0
Ile
3.49IleAla: 3.49 ± 0.208
1.446IleCys: 1.446 ± 0.121
7.711IleAsp: 7.711 ± 0.427
5.038IleGlu: 5.038 ± 0.274
3.797IlePhe: 3.797 ± 0.208
3.695IleGly: 3.695 ± 0.253
2.03IleHis: 2.03 ± 0.167
8.251IleIle: 8.251 ± 0.381
7.2IleLys: 7.2 ± 0.426
8.266IleLeu: 8.266 ± 0.363
2.161IleMet: 2.161 ± 0.178
7.58IleAsn: 7.58 ± 0.323
3.505IlePro: 3.505 ± 0.244
2.059IleGln: 2.059 ± 0.185
3.856IleArg: 3.856 ± 0.253
8.514IleSer: 8.514 ± 0.292
5.287IleThr: 5.287 ± 0.301
5.725IleVal: 5.725 ± 0.326
0.511IleTrp: 0.511 ± 0.082
4.513IleTyr: 4.513 ± 0.276
0.0IleXaa: 0.0 ± 0.0
Lys
1.884LysAla: 1.884 ± 0.201
1.913LysCys: 1.913 ± 0.243
4.732LysAsp: 4.732 ± 0.231
3.87LysGlu: 3.87 ± 0.238
2.95LysPhe: 2.95 ± 0.249
2.074LysGly: 2.074 ± 0.172
1.942LysHis: 1.942 ± 0.172
6.674LysIle: 6.674 ± 0.329
5.55LysLys: 5.55 ± 0.329
6.703LysLeu: 6.703 ± 0.239
1.84LysMet: 1.84 ± 0.165
5.112LysAsn: 5.112 ± 0.249
2.103LysPro: 2.103 ± 0.169
2.118LysGln: 2.118 ± 0.176
3.593LysArg: 3.593 ± 0.269
5.418LysSer: 5.418 ± 0.277
4.264LysThr: 4.264 ± 0.277
3.783LysVal: 3.783 ± 0.275
0.789LysTrp: 0.789 ± 0.134
4.761LysTyr: 4.761 ± 0.267
0.0LysXaa: 0.0 ± 0.0
Leu
3.228LeuAla: 3.228 ± 0.233
1.855LeuCys: 1.855 ± 0.199
5.886LeuAsp: 5.886 ± 0.253
5.082LeuGlu: 5.082 ± 0.371
4.542LeuPhe: 4.542 ± 0.272
3.111LeuGly: 3.111 ± 0.232
2.118LeuHis: 2.118 ± 0.286
6.572LeuIle: 6.572 ± 0.33
5.842LeuLys: 5.842 ± 0.319
8.748LeuLeu: 8.748 ± 0.408
2.468LeuMet: 2.468 ± 0.208
5.199LeuAsn: 5.199 ± 0.268
3.388LeuPro: 3.388 ± 0.257
1.84LeuGln: 1.84 ± 0.153
3.593LeuArg: 3.593 ± 0.275
7.638LeuSer: 7.638 ± 0.291
5.783LeuThr: 5.783 ± 0.302
5.564LeuVal: 5.564 ± 0.33
0.482LeuTrp: 0.482 ± 0.114
4.571LeuTyr: 4.571 ± 0.198
0.0LeuXaa: 0.0 ± 0.0
Met
1.46MetAla: 1.46 ± 0.145
0.584MetCys: 0.584 ± 0.086
2.103MetAsp: 2.103 ± 0.159
1.621MetGlu: 1.621 ± 0.142
1.256MetPhe: 1.256 ± 0.123
0.905MetGly: 0.905 ± 0.118
0.497MetHis: 0.497 ± 0.09
2.57MetIle: 2.57 ± 0.183
1.782MetLys: 1.782 ± 0.193
2.454MetLeu: 2.454 ± 0.178
0.92MetMet: 0.92 ± 0.118
1.855MetAsn: 1.855 ± 0.183
0.935MetPro: 0.935 ± 0.134
0.526MetGln: 0.526 ± 0.084
1.066MetArg: 1.066 ± 0.108
2.307MetSer: 2.307 ± 0.189
1.519MetThr: 1.519 ± 0.139
1.46MetVal: 1.46 ± 0.148
0.175MetTrp: 0.175 ± 0.056
1.636MetTyr: 1.636 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
2.775AsnAla: 2.775 ± 0.184
1.285AsnCys: 1.285 ± 0.157
4.6AsnAsp: 4.6 ± 0.278
3.651AsnGlu: 3.651 ± 0.248
2.468AsnPhe: 2.468 ± 0.204
3.33AsnGly: 3.33 ± 0.291
1.533AsnHis: 1.533 ± 0.166
7.872AsnIle: 7.872 ± 0.366
5.433AsnLys: 5.433 ± 0.304
4.732AsnLeu: 4.732 ± 0.246
2.147AsnMet: 2.147 ± 0.184
5.813AsnAsn: 5.813 ± 0.337
2.234AsnPro: 2.234 ± 0.152
1.3AsnGln: 1.3 ± 0.117
3.125AsnArg: 3.125 ± 0.229
4.396AsnSer: 4.396 ± 0.235
4.513AsnThr: 4.513 ± 0.269
4.264AsnVal: 4.264 ± 0.262
0.351AsnTrp: 0.351 ± 0.067
3.315AsnTyr: 3.315 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
1.139ProAla: 1.139 ± 0.134
0.584ProCys: 0.584 ± 0.093
2.001ProAsp: 2.001 ± 0.183
2.351ProGlu: 2.351 ± 0.16
1.519ProPhe: 1.519 ± 0.151
1.358ProGly: 1.358 ± 0.154
0.701ProHis: 0.701 ± 0.121
2.994ProIle: 2.994 ± 0.23
1.986ProLys: 1.986 ± 0.191
3.082ProLeu: 3.082 ± 0.218
0.92ProMet: 0.92 ± 0.098
2.103ProAsn: 2.103 ± 0.157
1.723ProPro: 1.723 ± 0.205
0.672ProGln: 0.672 ± 0.11
1.679ProArg: 1.679 ± 0.223
2.731ProSer: 2.731 ± 0.205
2.41ProThr: 2.41 ± 0.217
2.161ProVal: 2.161 ± 0.174
0.219ProTrp: 0.219 ± 0.057
1.606ProTyr: 1.606 ± 0.164
0.0ProXaa: 0.0 ± 0.0
Gln
0.657GlnAla: 0.657 ± 0.1
0.57GlnCys: 0.57 ± 0.104
1.271GlnAsp: 1.271 ± 0.113
1.241GlnGlu: 1.241 ± 0.148
0.935GlnPhe: 0.935 ± 0.099
0.555GlnGly: 0.555 ± 0.095
0.643GlnHis: 0.643 ± 0.096
1.519GlnIle: 1.519 ± 0.14
1.446GlnLys: 1.446 ± 0.165
2.366GlnLeu: 2.366 ± 0.189
0.701GlnMet: 0.701 ± 0.098
1.475GlnAsn: 1.475 ± 0.146
0.745GlnPro: 0.745 ± 0.148
0.876GlnGln: 0.876 ± 0.111
1.139GlnArg: 1.139 ± 0.152
1.431GlnSer: 1.431 ± 0.149
1.387GlnThr: 1.387 ± 0.13
0.964GlnVal: 0.964 ± 0.131
0.219GlnTrp: 0.219 ± 0.056
1.577GlnTyr: 1.577 ± 0.139
0.0GlnXaa: 0.0 ± 0.0
Arg
1.212ArgAla: 1.212 ± 0.127
1.066ArgCys: 1.066 ± 0.128
3.052ArgAsp: 3.052 ± 0.236
2.264ArgGlu: 2.264 ± 0.331
2.176ArgPhe: 2.176 ± 0.204
1.899ArgGly: 1.899 ± 0.159
1.329ArgHis: 1.329 ± 0.152
3.388ArgIle: 3.388 ± 0.229
2.789ArgLys: 2.789 ± 0.197
4.206ArgLeu: 4.206 ± 0.262
1.066ArgMet: 1.066 ± 0.125
2.921ArgAsn: 2.921 ± 0.212
1.446ArgPro: 1.446 ± 0.18
1.431ArgGln: 1.431 ± 0.149
2.629ArgArg: 2.629 ± 0.226
3.125ArgSer: 3.125 ± 0.211
2.234ArgThr: 2.234 ± 0.191
2.541ArgVal: 2.541 ± 0.171
0.409ArgTrp: 0.409 ± 0.079
2.658ArgTyr: 2.658 ± 0.229
0.0ArgXaa: 0.0 ± 0.0
Ser
2.877SerAla: 2.877 ± 0.208
1.606SerCys: 1.606 ± 0.18
4.965SerAsp: 4.965 ± 0.335
3.739SerGlu: 3.739 ± 0.265
3.709SerPhe: 3.709 ± 0.258
3.447SerGly: 3.447 ± 0.263
1.431SerHis: 1.431 ± 0.116
7.229SerIle: 7.229 ± 0.317
6.09SerLys: 6.09 ± 0.32
7.142SerLeu: 7.142 ± 0.332
2.322SerMet: 2.322 ± 0.212
4.761SerAsn: 4.761 ± 0.275
2.775SerPro: 2.775 ± 0.214
1.942SerGln: 1.942 ± 0.188
3.271SerArg: 3.271 ± 0.225
6.82SerSer: 6.82 ± 0.373
4.878SerThr: 4.878 ± 0.335
4.98SerVal: 4.98 ± 0.288
0.351SerTrp: 0.351 ± 0.079
3.68SerTyr: 3.68 ± 0.229
0.0SerXaa: 0.0 ± 0.0
Thr
2.439ThrAla: 2.439 ± 0.217
1.417ThrCys: 1.417 ± 0.165
4.279ThrAsp: 4.279 ± 0.227
3.067ThrGlu: 3.067 ± 0.229
2.629ThrPhe: 2.629 ± 0.21
2.191ThrGly: 2.191 ± 0.186
1.314ThrHis: 1.314 ± 0.151
5.988ThrIle: 5.988 ± 0.28
4.367ThrLys: 4.367 ± 0.274
4.761ThrLeu: 4.761 ± 0.24
1.796ThrMet: 1.796 ± 0.185
3.826ThrAsn: 3.826 ± 0.245
2.877ThrPro: 2.877 ± 0.319
1.125ThrGln: 1.125 ± 0.146
2.337ThrArg: 2.337 ± 0.152
4.849ThrSer: 4.849 ± 0.309
4.016ThrThr: 4.016 ± 0.268
4.075ThrVal: 4.075 ± 0.259
0.526ThrTrp: 0.526 ± 0.078
3.052ThrTyr: 3.052 ± 0.181
0.0ThrXaa: 0.0 ± 0.0
Val
2.278ValAla: 2.278 ± 0.181
1.563ValCys: 1.563 ± 0.172
4.44ValAsp: 4.44 ± 0.293
3.52ValGlu: 3.52 ± 0.243
3.125ValPhe: 3.125 ± 0.247
1.855ValGly: 1.855 ± 0.188
1.008ValHis: 1.008 ± 0.115
5.52ValIle: 5.52 ± 0.259
4.965ValLys: 4.965 ± 0.268
5.17ValLeu: 5.17 ± 0.273
1.49ValMet: 1.49 ± 0.152
4.6ValAsn: 4.6 ± 0.242
1.928ValPro: 1.928 ± 0.198
1.154ValGln: 1.154 ± 0.103
2.6ValArg: 2.6 ± 0.164
4.892ValSer: 4.892 ± 0.218
3.739ValThr: 3.739 ± 0.292
3.666ValVal: 3.666 ± 0.237
0.234ValTrp: 0.234 ± 0.054
3.461ValTyr: 3.461 ± 0.251
0.0ValXaa: 0.0 ± 0.0
Trp
0.175TrpAla: 0.175 ± 0.05
0.161TrpCys: 0.161 ± 0.041
0.277TrpAsp: 0.277 ± 0.064
0.424TrpGlu: 0.424 ± 0.091
0.38TrpPhe: 0.38 ± 0.077
0.234TrpGly: 0.234 ± 0.054
0.117TrpHis: 0.117 ± 0.038
0.599TrpIle: 0.599 ± 0.104
0.657TrpLys: 0.657 ± 0.112
0.73TrpLeu: 0.73 ± 0.106
0.38TrpMet: 0.38 ± 0.067
0.438TrpAsn: 0.438 ± 0.093
0.263TrpPro: 0.263 ± 0.052
0.175TrpGln: 0.175 ± 0.054
0.292TrpArg: 0.292 ± 0.068
0.424TrpSer: 0.424 ± 0.077
0.409TrpThr: 0.409 ± 0.078
0.307TrpVal: 0.307 ± 0.065
0.0TrpTrp: 0.0 ± 0.0
0.365TrpTyr: 0.365 ± 0.064
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.161TyrAla: 2.161 ± 0.169
1.344TyrCys: 1.344 ± 0.144
3.33TyrAsp: 3.33 ± 0.25
2.468TyrGlu: 2.468 ± 0.179
2.629TyrPhe: 2.629 ± 0.175
2.468TyrGly: 2.468 ± 0.176
1.212TyrHis: 1.212 ± 0.112
5.798TyrIle: 5.798 ± 0.277
3.929TyrLys: 3.929 ± 0.234
5.082TyrLeu: 5.082 ± 0.234
1.563TyrMet: 1.563 ± 0.16
4.133TyrAsn: 4.133 ± 0.304
1.665TyrPro: 1.665 ± 0.154
1.008TyrGln: 1.008 ± 0.125
2.161TyrArg: 2.161 ± 0.199
4.031TyrSer: 4.031 ± 0.235
3.14TyrThr: 3.14 ± 0.25
2.979TyrVal: 2.979 ± 0.226
0.351TyrTrp: 0.351 ± 0.076
3.155TyrTyr: 3.155 ± 0.239
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 226 proteins (68474 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski