Amino acid dipepetide frequency for Mycobacterium phage Seabiscuit

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.579AlaAla: 13.579 ± 1.498
0.614AlaCys: 0.614 ± 0.174
7.005AlaAsp: 7.005 ± 0.671
6.267AlaGlu: 6.267 ± 0.771
3.687AlaPhe: 3.687 ± 0.494
8.479AlaGly: 8.479 ± 0.855
1.475AlaHis: 1.475 ± 0.336
4.363AlaIle: 4.363 ± 0.468
4.363AlaLys: 4.363 ± 0.482
8.664AlaLeu: 8.664 ± 0.826
2.704AlaMet: 2.704 ± 0.408
2.642AlaAsn: 2.642 ± 0.423
4.793AlaPro: 4.793 ± 0.69
3.318AlaGln: 3.318 ± 0.492
6.513AlaArg: 6.513 ± 0.599
5.653AlaSer: 5.653 ± 0.65
5.038AlaThr: 5.038 ± 0.612
8.848AlaVal: 8.848 ± 0.885
1.72AlaTrp: 1.72 ± 0.307
3.072AlaTyr: 3.072 ± 0.444
0.0AlaXaa: 0.0 ± 0.0
Cys
0.676CysAla: 0.676 ± 0.216
0.0CysCys: 0.0 ± 0.0
0.492CysAsp: 0.492 ± 0.151
0.676CysGlu: 0.676 ± 0.223
0.123CysPhe: 0.123 ± 0.076
0.737CysGly: 0.737 ± 0.264
0.184CysHis: 0.184 ± 0.108
0.184CysIle: 0.184 ± 0.097
0.307CysLys: 0.307 ± 0.145
0.307CysLeu: 0.307 ± 0.14
0.123CysMet: 0.123 ± 0.087
0.369CysAsn: 0.369 ± 0.17
0.369CysPro: 0.369 ± 0.158
0.184CysGln: 0.184 ± 0.101
0.553CysArg: 0.553 ± 0.161
0.553CysSer: 0.553 ± 0.17
0.246CysThr: 0.246 ± 0.105
0.246CysVal: 0.246 ± 0.13
0.307CysTrp: 0.307 ± 0.128
0.184CysTyr: 0.184 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
6.636AspAla: 6.636 ± 0.676
0.492AspCys: 0.492 ± 0.176
4.24AspAsp: 4.24 ± 0.541
3.871AspGlu: 3.871 ± 0.494
2.212AspPhe: 2.212 ± 0.366
6.329AspGly: 6.329 ± 0.502
1.167AspHis: 1.167 ± 0.254
2.826AspIle: 2.826 ± 0.409
2.335AspLys: 2.335 ± 0.416
7.066AspLeu: 7.066 ± 0.75
1.045AspMet: 1.045 ± 0.212
1.843AspAsn: 1.843 ± 0.322
5.284AspPro: 5.284 ± 0.575
1.598AspGln: 1.598 ± 0.359
3.687AspArg: 3.687 ± 0.391
3.134AspSer: 3.134 ± 0.504
3.81AspThr: 3.81 ± 0.452
4.363AspVal: 4.363 ± 0.56
1.905AspTrp: 1.905 ± 0.339
1.966AspTyr: 1.966 ± 0.347
0.0AspXaa: 0.0 ± 0.0
Glu
5.776GluAla: 5.776 ± 0.764
0.43GluCys: 0.43 ± 0.171
4.854GluAsp: 4.854 ± 0.537
5.161GluGlu: 5.161 ± 0.728
2.273GluPhe: 2.273 ± 0.337
3.564GluGly: 3.564 ± 0.441
1.475GluHis: 1.475 ± 0.336
3.318GluIle: 3.318 ± 0.472
2.826GluLys: 2.826 ± 0.447
6.697GluLeu: 6.697 ± 0.618
1.782GluMet: 1.782 ± 0.331
1.843GluAsn: 1.843 ± 0.344
2.458GluPro: 2.458 ± 0.404
2.151GluGln: 2.151 ± 0.361
3.932GluArg: 3.932 ± 0.583
3.441GluSer: 3.441 ± 0.378
3.81GluThr: 3.81 ± 0.531
5.346GluVal: 5.346 ± 0.579
1.659GluTrp: 1.659 ± 0.344
2.826GluTyr: 2.826 ± 0.497
0.0GluXaa: 0.0 ± 0.0
Phe
2.519PheAla: 2.519 ± 0.354
0.369PheCys: 0.369 ± 0.162
2.949PheAsp: 2.949 ± 0.427
1.966PheGlu: 1.966 ± 0.365
0.553PhePhe: 0.553 ± 0.171
3.257PheGly: 3.257 ± 0.477
0.799PheHis: 0.799 ± 0.246
1.29PheIle: 1.29 ± 0.294
1.29PheLys: 1.29 ± 0.276
2.642PheLeu: 2.642 ± 0.409
0.737PheMet: 0.737 ± 0.231
1.045PheAsn: 1.045 ± 0.238
1.659PhePro: 1.659 ± 0.31
0.922PheGln: 0.922 ± 0.232
1.905PheArg: 1.905 ± 0.318
2.151PheSer: 2.151 ± 0.456
1.843PheThr: 1.843 ± 0.3
2.273PheVal: 2.273 ± 0.349
0.737PheTrp: 0.737 ± 0.211
0.983PheTyr: 0.983 ± 0.231
0.0PheXaa: 0.0 ± 0.0
Gly
7.68GlyAla: 7.68 ± 0.968
0.553GlyCys: 0.553 ± 0.18
5.53GlyAsp: 5.53 ± 0.586
4.547GlyGlu: 4.547 ± 0.52
2.704GlyPhe: 2.704 ± 0.464
9.524GlyGly: 9.524 ± 2.38
1.966GlyHis: 1.966 ± 0.352
4.547GlyIle: 4.547 ± 0.723
3.502GlyLys: 3.502 ± 0.45
7.865GlyLeu: 7.865 ± 0.891
1.72GlyMet: 1.72 ± 0.298
3.195GlyAsn: 3.195 ± 0.406
3.748GlyPro: 3.748 ± 0.528
2.273GlyGln: 2.273 ± 0.34
4.854GlyArg: 4.854 ± 0.556
6.206GlySer: 6.206 ± 0.858
5.407GlyThr: 5.407 ± 0.607
5.161GlyVal: 5.161 ± 0.592
2.519GlyTrp: 2.519 ± 0.386
3.195GlyTyr: 3.195 ± 0.436
0.0GlyXaa: 0.0 ± 0.0
His
1.843HisAla: 1.843 ± 0.311
0.184HisCys: 0.184 ± 0.129
1.229HisAsp: 1.229 ± 0.244
1.843HisGlu: 1.843 ± 0.38
0.737HisPhe: 0.737 ± 0.235
1.659HisGly: 1.659 ± 0.392
0.737HisHis: 0.737 ± 0.206
0.737HisIle: 0.737 ± 0.196
1.045HisLys: 1.045 ± 0.288
1.29HisLeu: 1.29 ± 0.276
0.123HisMet: 0.123 ± 0.089
0.369HisAsn: 0.369 ± 0.15
1.352HisPro: 1.352 ± 0.227
0.922HisGln: 0.922 ± 0.223
1.659HisArg: 1.659 ± 0.284
0.737HisSer: 0.737 ± 0.21
1.045HisThr: 1.045 ± 0.251
1.782HisVal: 1.782 ± 0.304
0.492HisTrp: 0.492 ± 0.161
0.614HisTyr: 0.614 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
6.267IleAla: 6.267 ± 0.805
0.246IleCys: 0.246 ± 0.124
2.888IleAsp: 2.888 ± 0.314
3.564IleGlu: 3.564 ± 0.406
1.167IlePhe: 1.167 ± 0.217
3.564IleGly: 3.564 ± 0.497
0.983IleHis: 0.983 ± 0.247
1.782IleIle: 1.782 ± 0.293
1.966IleLys: 1.966 ± 0.269
3.379IleLeu: 3.379 ± 0.499
0.799IleMet: 0.799 ± 0.199
2.028IleAsn: 2.028 ± 0.304
3.072IlePro: 3.072 ± 0.421
1.413IleGln: 1.413 ± 0.372
4.24IleArg: 4.24 ± 0.497
3.257IleSer: 3.257 ± 0.488
3.257IleThr: 3.257 ± 0.451
3.072IleVal: 3.072 ± 0.533
0.737IleTrp: 0.737 ± 0.183
1.782IleTyr: 1.782 ± 0.284
0.0IleXaa: 0.0 ± 0.0
Lys
3.994LysAla: 3.994 ± 0.491
0.369LysCys: 0.369 ± 0.163
2.888LysAsp: 2.888 ± 0.448
2.089LysGlu: 2.089 ± 0.357
1.598LysPhe: 1.598 ± 0.298
2.642LysGly: 2.642 ± 0.396
1.229LysHis: 1.229 ± 0.288
2.396LysIle: 2.396 ± 0.392
2.151LysLys: 2.151 ± 0.387
3.318LysLeu: 3.318 ± 0.42
0.737LysMet: 0.737 ± 0.198
1.229LysAsn: 1.229 ± 0.249
2.765LysPro: 2.765 ± 0.406
1.659LysGln: 1.659 ± 0.314
3.011LysArg: 3.011 ± 0.455
2.151LysSer: 2.151 ± 0.386
2.273LysThr: 2.273 ± 0.445
3.072LysVal: 3.072 ± 0.419
0.737LysTrp: 0.737 ± 0.232
0.983LysTyr: 0.983 ± 0.233
0.0LysXaa: 0.0 ± 0.0
Leu
9.831LeuAla: 9.831 ± 0.786
0.492LeuCys: 0.492 ± 0.17
6.022LeuAsp: 6.022 ± 0.574
5.346LeuGlu: 5.346 ± 0.598
2.028LeuPhe: 2.028 ± 0.385
7.312LeuGly: 7.312 ± 0.758
1.475LeuHis: 1.475 ± 0.303
5.284LeuIle: 5.284 ± 0.553
3.81LeuLys: 3.81 ± 0.498
5.53LeuLeu: 5.53 ± 0.561
1.966LeuMet: 1.966 ± 0.28
2.888LeuAsn: 2.888 ± 0.376
5.53LeuPro: 5.53 ± 0.624
2.581LeuGln: 2.581 ± 0.426
5.653LeuArg: 5.653 ± 0.523
5.469LeuSer: 5.469 ± 0.513
5.653LeuThr: 5.653 ± 0.543
4.485LeuVal: 4.485 ± 0.592
1.106LeuTrp: 1.106 ± 0.316
2.151LeuTyr: 2.151 ± 0.409
0.0LeuXaa: 0.0 ± 0.0
Met
2.335MetAla: 2.335 ± 0.323
0.0MetCys: 0.0 ± 0.0
1.229MetAsp: 1.229 ± 0.23
1.598MetGlu: 1.598 ± 0.3
0.676MetPhe: 0.676 ± 0.204
1.352MetGly: 1.352 ± 0.299
0.43MetHis: 0.43 ± 0.147
0.799MetIle: 0.799 ± 0.231
0.922MetLys: 0.922 ± 0.219
1.167MetLeu: 1.167 ± 0.234
0.184MetMet: 0.184 ± 0.096
0.983MetAsn: 0.983 ± 0.22
1.29MetPro: 1.29 ± 0.248
0.369MetGln: 0.369 ± 0.153
1.413MetArg: 1.413 ± 0.297
2.151MetSer: 2.151 ± 0.355
2.212MetThr: 2.212 ± 0.282
1.045MetVal: 1.045 ± 0.255
0.184MetTrp: 0.184 ± 0.091
0.492MetTyr: 0.492 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.379AsnAla: 3.379 ± 0.495
0.123AsnCys: 0.123 ± 0.095
2.212AsnAsp: 2.212 ± 0.384
1.966AsnGlu: 1.966 ± 0.355
1.045AsnPhe: 1.045 ± 0.257
3.318AsnGly: 3.318 ± 0.466
0.553AsnHis: 0.553 ± 0.142
1.536AsnIle: 1.536 ± 0.348
0.553AsnLys: 0.553 ± 0.172
2.335AsnLeu: 2.335 ± 0.332
0.614AsnMet: 0.614 ± 0.172
1.045AsnAsn: 1.045 ± 0.344
2.949AsnPro: 2.949 ± 0.355
1.106AsnGln: 1.106 ± 0.276
1.72AsnArg: 1.72 ± 0.395
1.659AsnSer: 1.659 ± 0.32
1.782AsnThr: 1.782 ± 0.299
2.273AsnVal: 2.273 ± 0.4
0.614AsnTrp: 0.614 ± 0.173
1.167AsnTyr: 1.167 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
5.284ProAla: 5.284 ± 0.525
0.614ProCys: 0.614 ± 0.22
4.424ProAsp: 4.424 ± 0.51
4.485ProGlu: 4.485 ± 0.486
2.151ProPhe: 2.151 ± 0.378
4.731ProGly: 4.731 ± 0.545
1.045ProHis: 1.045 ± 0.257
2.581ProIle: 2.581 ± 0.404
2.028ProLys: 2.028 ± 0.262
4.608ProLeu: 4.608 ± 0.523
0.922ProMet: 0.922 ± 0.257
1.72ProAsn: 1.72 ± 0.302
2.396ProPro: 2.396 ± 0.459
1.598ProGln: 1.598 ± 0.375
2.949ProArg: 2.949 ± 0.477
3.502ProSer: 3.502 ± 0.471
3.932ProThr: 3.932 ± 0.566
3.871ProVal: 3.871 ± 0.487
0.676ProTrp: 0.676 ± 0.226
1.536ProTyr: 1.536 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
3.195GlnAla: 3.195 ± 0.464
0.307GlnCys: 0.307 ± 0.148
1.229GlnAsp: 1.229 ± 0.309
1.72GlnGlu: 1.72 ± 0.292
1.045GlnPhe: 1.045 ± 0.211
2.704GlnGly: 2.704 ± 0.306
0.553GlnHis: 0.553 ± 0.16
2.765GlnIle: 2.765 ± 0.542
0.983GlnLys: 0.983 ± 0.203
3.871GlnLeu: 3.871 ± 0.555
0.922GlnMet: 0.922 ± 0.216
0.676GlnAsn: 0.676 ± 0.203
1.536GlnPro: 1.536 ± 0.271
1.843GlnGln: 1.843 ± 0.389
1.966GlnArg: 1.966 ± 0.334
1.598GlnSer: 1.598 ± 0.288
1.475GlnThr: 1.475 ± 0.246
2.458GlnVal: 2.458 ± 0.366
0.614GlnTrp: 0.614 ± 0.141
0.492GlnTyr: 0.492 ± 0.147
0.0GlnXaa: 0.0 ± 0.0
Arg
6.267ArgAla: 6.267 ± 0.625
0.737ArgCys: 0.737 ± 0.241
2.888ArgAsp: 2.888 ± 0.409
4.301ArgGlu: 4.301 ± 0.502
1.966ArgPhe: 1.966 ± 0.383
5.038ArgGly: 5.038 ± 0.615
1.167ArgHis: 1.167 ± 0.282
2.888ArgIle: 2.888 ± 0.488
3.564ArgLys: 3.564 ± 0.529
5.837ArgLeu: 5.837 ± 0.728
1.905ArgMet: 1.905 ± 0.389
2.273ArgAsn: 2.273 ± 0.436
2.581ArgPro: 2.581 ± 0.404
2.151ArgGln: 2.151 ± 0.345
5.1ArgArg: 5.1 ± 0.669
3.441ArgSer: 3.441 ± 0.476
3.195ArgThr: 3.195 ± 0.504
5.038ArgVal: 5.038 ± 0.496
1.413ArgTrp: 1.413 ± 0.305
2.151ArgTyr: 2.151 ± 0.322
0.0ArgXaa: 0.0 ± 0.0
Ser
5.653SerAla: 5.653 ± 0.603
0.43SerCys: 0.43 ± 0.189
3.134SerAsp: 3.134 ± 0.376
3.81SerGlu: 3.81 ± 0.516
1.843SerPhe: 1.843 ± 0.375
6.943SerGly: 6.943 ± 1.077
1.413SerHis: 1.413 ± 0.321
2.826SerIle: 2.826 ± 0.402
2.335SerLys: 2.335 ± 0.331
4.977SerLeu: 4.977 ± 0.564
1.598SerMet: 1.598 ± 0.351
2.151SerAsn: 2.151 ± 0.392
3.195SerPro: 3.195 ± 0.558
1.659SerGln: 1.659 ± 0.298
3.072SerArg: 3.072 ± 0.431
3.134SerSer: 3.134 ± 0.607
3.011SerThr: 3.011 ± 0.48
3.81SerVal: 3.81 ± 0.395
1.536SerTrp: 1.536 ± 0.335
1.475SerTyr: 1.475 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
6.82ThrAla: 6.82 ± 0.95
0.307ThrCys: 0.307 ± 0.15
4.117ThrAsp: 4.117 ± 0.502
4.24ThrGlu: 4.24 ± 0.514
2.273ThrPhe: 2.273 ± 0.357
6.759ThrGly: 6.759 ± 0.544
1.045ThrHis: 1.045 ± 0.322
3.011ThrIle: 3.011 ± 0.528
2.519ThrLys: 2.519 ± 0.378
5.714ThrLeu: 5.714 ± 0.592
0.676ThrMet: 0.676 ± 0.2
1.598ThrAsn: 1.598 ± 0.302
3.441ThrPro: 3.441 ± 0.43
1.598ThrGln: 1.598 ± 0.294
2.888ThrArg: 2.888 ± 0.502
2.949ThrSer: 2.949 ± 0.567
4.547ThrThr: 4.547 ± 0.6
4.608ThrVal: 4.608 ± 0.532
1.045ThrTrp: 1.045 ± 0.31
1.72ThrTyr: 1.72 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
6.759ValAla: 6.759 ± 0.68
0.246ValCys: 0.246 ± 0.108
5.653ValAsp: 5.653 ± 0.591
4.608ValGlu: 4.608 ± 0.541
2.212ValPhe: 2.212 ± 0.319
4.67ValGly: 4.67 ± 0.75
1.536ValHis: 1.536 ± 0.305
3.81ValIle: 3.81 ± 0.449
3.195ValLys: 3.195 ± 0.471
4.854ValLeu: 4.854 ± 0.622
1.045ValMet: 1.045 ± 0.292
2.335ValAsn: 2.335 ± 0.308
4.055ValPro: 4.055 ± 0.463
2.396ValGln: 2.396 ± 0.409
5.223ValArg: 5.223 ± 0.586
4.608ValSer: 4.608 ± 0.427
5.469ValThr: 5.469 ± 0.563
4.977ValVal: 4.977 ± 0.56
1.167ValTrp: 1.167 ± 0.263
2.089ValTyr: 2.089 ± 0.362
0.0ValXaa: 0.0 ± 0.0
Trp
1.72TrpAla: 1.72 ± 0.339
0.184TrpCys: 0.184 ± 0.109
1.29TrpAsp: 1.29 ± 0.234
1.106TrpGlu: 1.106 ± 0.202
0.86TrpPhe: 0.86 ± 0.221
1.598TrpGly: 1.598 ± 0.293
0.492TrpHis: 0.492 ± 0.168
1.167TrpIle: 1.167 ± 0.243
0.307TrpLys: 0.307 ± 0.169
1.966TrpLeu: 1.966 ± 0.288
0.43TrpMet: 0.43 ± 0.145
0.369TrpAsn: 0.369 ± 0.155
0.86TrpPro: 0.86 ± 0.244
1.106TrpGln: 1.106 ± 0.251
1.106TrpArg: 1.106 ± 0.291
0.922TrpSer: 0.922 ± 0.234
1.659TrpThr: 1.659 ± 0.328
2.151TrpVal: 2.151 ± 0.333
0.492TrpTrp: 0.492 ± 0.209
0.369TrpTyr: 0.369 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.581TyrAla: 2.581 ± 0.434
0.123TyrCys: 0.123 ± 0.098
1.536TyrAsp: 1.536 ± 0.323
2.458TyrGlu: 2.458 ± 0.384
0.737TyrPhe: 0.737 ± 0.178
2.519TyrGly: 2.519 ± 0.393
0.676TyrHis: 0.676 ± 0.207
1.413TyrIle: 1.413 ± 0.311
1.29TyrLys: 1.29 ± 0.28
2.519TyrLeu: 2.519 ± 0.463
0.737TyrMet: 0.737 ± 0.173
1.352TyrAsn: 1.352 ± 0.29
1.72TyrPro: 1.72 ± 0.3
1.106TyrGln: 1.106 ± 0.241
2.458TyrArg: 2.458 ± 0.352
1.29TyrSer: 1.29 ± 0.229
2.212TyrThr: 2.212 ± 0.361
2.028TyrVal: 2.028 ± 0.381
0.492TyrTrp: 0.492 ± 0.18
0.737TyrTyr: 0.737 ± 0.207
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (16276 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski