Sulfitobacter donghicola DSW-25 = KCTC 12864 = JCM 14565

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter; Sulfitobacter donghicola

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3416 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A073IGL0|A0A073IGL0_9RHOB Glycine/betaine ABC transporter OS=Sulfitobacter donghicola DSW-25 = KCTC 12864 = JCM 14565 OX=1300350 GN=DSW25_13230 PE=3 SV=1
MM1 pKa = 8.08IEE3 pKa = 4.09VEE5 pKa = 4.27LLQAIEE11 pKa = 4.3SGQNVTSGHH20 pKa = 6.09FGGNVIAGKK29 pKa = 8.61NTDD32 pKa = 4.02DD33 pKa = 6.16GIPTEE38 pKa = 4.32QNSLAHH44 pKa = 6.16QEE46 pKa = 3.81LDD48 pKa = 3.27IEE50 pKa = 4.62VARR53 pKa = 11.84YY54 pKa = 7.69PAGEE58 pKa = 4.53PDD60 pKa = 4.81LMYY63 pKa = 10.84KK64 pKa = 10.5DD65 pKa = 4.01GMVINGALPDD75 pKa = 3.79HH76 pKa = 7.16LINFLMAARR85 pKa = 11.84ASGQSVVLVTPTHH98 pKa = 6.2EE99 pKa = 5.18GYY101 pKa = 10.5HH102 pKa = 5.9GADD105 pKa = 2.97ILTEE109 pKa = 4.15FTSLVLGQFSDD120 pKa = 4.14VIHH123 pKa = 6.63AFEE126 pKa = 5.61IGNEE130 pKa = 4.15YY131 pKa = 9.98WNHH134 pKa = 4.16QTEE137 pKa = 4.19TSYY140 pKa = 12.0GEE142 pKa = 4.07VANDD146 pKa = 3.22SVLAISSQLTGQYY159 pKa = 10.48GDD161 pKa = 3.21IPIWVQMGDD170 pKa = 3.04AGGQASEE177 pKa = 4.34FAKK180 pKa = 10.54DD181 pKa = 3.38APLSEE186 pKa = 4.91GIGWLWRR193 pKa = 11.84NIGANNLILDD203 pKa = 3.79QLTPEE208 pKa = 4.08ARR210 pKa = 11.84ATIDD214 pKa = 3.34GVVEE218 pKa = 4.12HH219 pKa = 6.67YY220 pKa = 10.55YY221 pKa = 10.65FRR223 pKa = 11.84EE224 pKa = 3.75DD225 pKa = 3.03HH226 pKa = 6.51QYY228 pKa = 11.33LGFYY232 pKa = 10.11NDD234 pKa = 3.32QNIVMDD240 pKa = 3.94HH241 pKa = 6.12EE242 pKa = 4.94VWQSAFGRR250 pKa = 11.84NLTLNITEE258 pKa = 4.16WNIRR262 pKa = 11.84TTNLDD267 pKa = 3.19QLGIRR272 pKa = 11.84AASTLIAQFSFMMDD286 pKa = 3.28MEE288 pKa = 4.27VDD290 pKa = 3.57EE291 pKa = 5.74AYY293 pKa = 10.35VWPPMHH299 pKa = 6.23NTSTDD304 pKa = 3.33LAGSSNVLLDD314 pKa = 3.76PEE316 pKa = 4.41TGIVINSVGGATFDD330 pKa = 3.95LMSSSLVGLQYY341 pKa = 10.55RR342 pKa = 11.84PSATTSDD349 pKa = 3.11SSLLHH354 pKa = 6.17NYY356 pKa = 9.26VYY358 pKa = 10.72SGEE361 pKa = 4.35DD362 pKa = 2.94KK363 pKa = 10.72VVVYY367 pKa = 7.6VTSRR371 pKa = 11.84SDD373 pKa = 3.21EE374 pKa = 4.3TEE376 pKa = 3.82SVSFNLGNFFPGASLISATQIGYY399 pKa = 10.26DD400 pKa = 3.44KK401 pKa = 10.37TSSDD405 pKa = 3.43GRR407 pKa = 11.84HH408 pKa = 5.31YY409 pKa = 11.13DD410 pKa = 3.36YY411 pKa = 11.26VQRR414 pKa = 11.84EE415 pKa = 4.35FVDD418 pKa = 3.97SEE420 pKa = 4.01AVAIDD425 pKa = 3.52GEE427 pKa = 4.4LYY429 pKa = 8.87YY430 pKa = 10.88TNEE433 pKa = 3.79HH434 pKa = 6.79DD435 pKa = 3.95VRR437 pKa = 11.84ATITEE442 pKa = 4.32HH443 pKa = 6.9DD444 pKa = 3.96TSQSTNGGNFTFTLLPYY461 pKa = 10.21EE462 pKa = 4.55VIEE465 pKa = 4.14LTYY468 pKa = 9.85EE469 pKa = 3.63IPNFEE474 pKa = 4.42RR475 pKa = 11.84TDD477 pKa = 3.44GTNGKK482 pKa = 9.86DD483 pKa = 3.2IISSEE488 pKa = 4.04DD489 pKa = 3.32SKK491 pKa = 11.92NDD493 pKa = 3.5LVFSLAGNDD502 pKa = 3.7NIQAGSGDD510 pKa = 3.63DD511 pKa = 3.79SIYY514 pKa = 11.17AGDD517 pKa = 3.87GDD519 pKa = 4.87DD520 pKa = 5.65YY521 pKa = 11.62IDD523 pKa = 5.23AGLGDD528 pKa = 4.66DD529 pKa = 4.62LVSGGAGNDD538 pKa = 3.52IIRR541 pKa = 11.84GRR543 pKa = 11.84GNNDD547 pKa = 3.33IISGGSGNDD556 pKa = 4.37DD557 pKa = 3.47IDD559 pKa = 4.99GGWGDD564 pKa = 4.66DD565 pKa = 3.97KK566 pKa = 11.73LDD568 pKa = 4.06GGSGSDD574 pKa = 3.5ILNGGWGRR582 pKa = 11.84DD583 pKa = 3.64TITLDD588 pKa = 3.11SSLDD592 pKa = 3.4IAIGGGDD599 pKa = 4.26DD600 pKa = 6.54DD601 pKa = 6.27LFQLDD606 pKa = 3.9GLQKK610 pKa = 10.87YY611 pKa = 8.21GAGWGGINASNQYY624 pKa = 10.51QDD626 pKa = 2.91GTMVFLPVANYY637 pKa = 10.23NLYY640 pKa = 10.64SSVIIGGAGYY650 pKa = 8.22DD651 pKa = 3.89TIEE654 pKa = 4.98LSASDD659 pKa = 4.66DD660 pKa = 4.89AIFLHH665 pKa = 6.56NGFSSLPSSLNVSEE679 pKa = 4.58YY680 pKa = 11.26VSDD683 pKa = 4.07LPSGLMISGIEE694 pKa = 4.06EE695 pKa = 3.77FRR697 pKa = 11.84AGAGNDD703 pKa = 3.8FVDD706 pKa = 3.97LTSDD710 pKa = 3.57VFHH713 pKa = 6.89LQGQSIVIHH722 pKa = 6.69GDD724 pKa = 3.11YY725 pKa = 10.93GHH727 pKa = 7.0DD728 pKa = 3.95VVWGSAANEE737 pKa = 4.34TIFGGEE743 pKa = 4.19GNDD746 pKa = 3.7TLFGGAGNDD755 pKa = 3.82EE756 pKa = 4.22ISGGTGADD764 pKa = 3.08VFEE767 pKa = 4.52FTSTSIDD774 pKa = 3.24TSLTDD779 pKa = 3.61FDD781 pKa = 4.12VRR783 pKa = 11.84EE784 pKa = 4.09GDD786 pKa = 3.39EE787 pKa = 3.76LRR789 pKa = 11.84FYY791 pKa = 10.13NTEE794 pKa = 3.76NVEE797 pKa = 3.95FDD799 pKa = 3.59AGSVALTPGGINISYY814 pKa = 10.07RR815 pKa = 11.84NTVSEE820 pKa = 4.27TEE822 pKa = 3.99HH823 pKa = 7.37YY824 pKa = 10.72IFISLVTHH832 pKa = 5.1STEE835 pKa = 4.06FAATLPEE842 pKa = 4.11ILNALEE848 pKa = 4.16IVV850 pKa = 3.55

Molecular weight: 91.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A073IFB4|A0A073IFB4_9RHOB Radical SAM protein OS=Sulfitobacter donghicola DSW-25 = KCTC 12864 = JCM 14565 OX=1300350 GN=DSW25_15235 PE=4 SV=1
MM1 pKa = 7.42NLTAFANASPVIQIHH16 pKa = 5.32TLLALGAVLLTISIFALRR34 pKa = 11.84KK35 pKa = 9.17GSPLHH40 pKa = 6.93RR41 pKa = 11.84VMGWTWVIMMALVALSSFWINEE63 pKa = 3.57LRR65 pKa = 11.84VLGAFSPIHH74 pKa = 6.2LLSIITLVTLVSSVTAARR92 pKa = 11.84RR93 pKa = 11.84HH94 pKa = 4.2NVKK97 pKa = 9.62RR98 pKa = 11.84HH99 pKa = 4.21KK100 pKa = 10.36RR101 pKa = 11.84QMRR104 pKa = 11.84TLVFGALIVAGGFTFLPGRR123 pKa = 11.84LMHH126 pKa = 6.07TVFLGGG132 pKa = 3.45

Molecular weight: 14.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3416

0

3416

1055224

29

6491

308.9

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.669 ± 0.049

0.884 ± 0.014

6.096 ± 0.041

6.075 ± 0.04

3.816 ± 0.027

8.437 ± 0.042

2.045 ± 0.023

5.472 ± 0.029

3.815 ± 0.04

9.682 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.886 ± 0.027

2.983 ± 0.024

4.767 ± 0.031

3.486 ± 0.023

5.957 ± 0.047

5.537 ± 0.032

5.646 ± 0.059

7.127 ± 0.041

1.353 ± 0.018

2.268 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski