Amino acid dipepetide frequency for Mycobacterium phage Cain

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.797AlaAla: 20.797 ± 1.732
1.117AlaCys: 1.117 ± 0.301
8.351AlaAsp: 8.351 ± 0.731
9.095AlaGlu: 9.095 ± 0.891
3.138AlaPhe: 3.138 ± 0.524
9.414AlaGly: 9.414 ± 1.204
3.245AlaHis: 3.245 ± 0.427
5.319AlaIle: 5.319 ± 0.627
4.521AlaLys: 4.521 ± 0.508
12.659AlaLeu: 12.659 ± 0.901
3.351AlaMet: 3.351 ± 0.405
3.085AlaAsn: 3.085 ± 0.379
7.393AlaPro: 7.393 ± 0.786
4.415AlaGln: 4.415 ± 0.648
8.297AlaArg: 8.297 ± 0.748
4.893AlaSer: 4.893 ± 0.456
6.223AlaThr: 6.223 ± 0.791
9.946AlaVal: 9.946 ± 0.903
2.553AlaTrp: 2.553 ± 0.355
2.925AlaTyr: 2.925 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
1.17CysAla: 1.17 ± 0.273
0.106CysCys: 0.106 ± 0.069
0.585CysAsp: 0.585 ± 0.212
0.638CysGlu: 0.638 ± 0.196
0.266CysPhe: 0.266 ± 0.11
1.542CysGly: 1.542 ± 0.37
0.372CysHis: 0.372 ± 0.143
0.213CysIle: 0.213 ± 0.092
0.266CysLys: 0.266 ± 0.121
0.798CysLeu: 0.798 ± 0.202
0.16CysMet: 0.16 ± 0.103
0.266CysAsn: 0.266 ± 0.118
0.904CysPro: 0.904 ± 0.256
0.213CysGln: 0.213 ± 0.093
0.904CysArg: 0.904 ± 0.203
0.957CysSer: 0.957 ± 0.238
0.585CysThr: 0.585 ± 0.187
0.745CysVal: 0.745 ± 0.226
0.319CysTrp: 0.319 ± 0.151
0.213CysTyr: 0.213 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
8.404AspAla: 8.404 ± 0.563
0.745AspCys: 0.745 ± 0.209
5.212AspAsp: 5.212 ± 0.643
5.638AspGlu: 5.638 ± 0.622
0.904AspPhe: 0.904 ± 0.182
6.915AspGly: 6.915 ± 0.568
1.117AspHis: 1.117 ± 0.281
2.021AspIle: 2.021 ± 0.39
1.755AspLys: 1.755 ± 0.363
6.17AspLeu: 6.17 ± 0.64
1.755AspMet: 1.755 ± 0.334
1.755AspAsn: 1.755 ± 0.321
4.415AspPro: 4.415 ± 0.483
1.968AspGln: 1.968 ± 0.301
4.893AspArg: 4.893 ± 0.592
2.925AspSer: 2.925 ± 0.377
2.872AspThr: 2.872 ± 0.394
4.096AspVal: 4.096 ± 0.397
0.904AspTrp: 0.904 ± 0.195
1.489AspTyr: 1.489 ± 0.327
0.0AspXaa: 0.0 ± 0.0
Glu
8.297GluAla: 8.297 ± 1.061
0.957GluCys: 0.957 ± 0.224
2.393GluAsp: 2.393 ± 0.402
1.17GluGlu: 1.17 ± 0.263
2.128GluPhe: 2.128 ± 0.316
4.627GluGly: 4.627 ± 0.448
1.596GluHis: 1.596 ± 0.261
2.234GluIle: 2.234 ± 0.278
1.596GluLys: 1.596 ± 0.339
5.319GluLeu: 5.319 ± 0.593
1.17GluMet: 1.17 ± 0.246
1.436GluAsn: 1.436 ± 0.28
3.032GluPro: 3.032 ± 0.56
2.447GluGln: 2.447 ± 0.384
4.681GluArg: 4.681 ± 0.608
3.138GluSer: 3.138 ± 0.332
2.659GluThr: 2.659 ± 0.349
5.053GluVal: 5.053 ± 0.594
0.798GluTrp: 0.798 ± 0.205
2.181GluTyr: 2.181 ± 0.441
0.0GluXaa: 0.0 ± 0.0
Phe
3.138PheAla: 3.138 ± 0.518
0.266PheCys: 0.266 ± 0.117
2.872PheAsp: 2.872 ± 0.394
1.011PheGlu: 1.011 ± 0.194
0.638PhePhe: 0.638 ± 0.16
2.819PheGly: 2.819 ± 0.396
0.16PheHis: 0.16 ± 0.093
0.851PheIle: 0.851 ± 0.147
1.33PheLys: 1.33 ± 0.247
1.862PheLeu: 1.862 ± 0.304
0.532PheMet: 0.532 ± 0.139
1.436PheAsn: 1.436 ± 0.366
1.223PhePro: 1.223 ± 0.266
0.638PheGln: 0.638 ± 0.162
1.702PheArg: 1.702 ± 0.326
1.17PheSer: 1.17 ± 0.242
1.702PheThr: 1.702 ± 0.332
1.915PheVal: 1.915 ± 0.306
0.372PheTrp: 0.372 ± 0.145
0.532PheTyr: 0.532 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
10.106GlyAla: 10.106 ± 1.054
1.223GlyCys: 1.223 ± 0.299
5.957GlyAsp: 5.957 ± 0.56
5.957GlyGlu: 5.957 ± 0.539
1.915GlyPhe: 1.915 ± 0.374
8.457GlyGly: 8.457 ± 1.246
1.596GlyHis: 1.596 ± 0.295
2.234GlyIle: 2.234 ± 0.469
4.149GlyLys: 4.149 ± 0.59
6.915GlyLeu: 6.915 ± 0.827
2.128GlyMet: 2.128 ± 0.321
2.925GlyAsn: 2.925 ± 0.391
3.989GlyPro: 3.989 ± 0.545
2.287GlyGln: 2.287 ± 0.388
6.01GlyArg: 6.01 ± 0.653
5.212GlySer: 5.212 ± 0.575
6.117GlyThr: 6.117 ± 0.602
7.34GlyVal: 7.34 ± 0.637
2.393GlyTrp: 2.393 ± 0.314
2.34GlyTyr: 2.34 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
2.234HisAla: 2.234 ± 0.406
0.213HisCys: 0.213 ± 0.096
1.489HisAsp: 1.489 ± 0.359
1.064HisGlu: 1.064 ± 0.277
0.532HisPhe: 0.532 ± 0.148
2.393HisGly: 2.393 ± 0.41
0.585HisHis: 0.585 ± 0.155
0.851HisIle: 0.851 ± 0.191
0.532HisLys: 0.532 ± 0.156
1.968HisLeu: 1.968 ± 0.283
0.426HisMet: 0.426 ± 0.142
0.904HisAsn: 0.904 ± 0.21
1.383HisPro: 1.383 ± 0.275
0.479HisGln: 0.479 ± 0.167
2.181HisArg: 2.181 ± 0.406
1.011HisSer: 1.011 ± 0.2
1.064HisThr: 1.064 ± 0.231
2.713HisVal: 2.713 ± 0.365
0.372HisTrp: 0.372 ± 0.154
0.745HisTyr: 0.745 ± 0.203
0.0HisXaa: 0.0 ± 0.0
Ile
5.638IleAla: 5.638 ± 0.611
0.106IleCys: 0.106 ± 0.074
3.51IleAsp: 3.51 ± 0.391
3.032IleGlu: 3.032 ± 0.446
0.745IlePhe: 0.745 ± 0.211
4.574IleGly: 4.574 ± 0.741
0.638IleHis: 0.638 ± 0.218
0.745IleIle: 0.745 ± 0.244
1.277IleLys: 1.277 ± 0.257
2.553IleLeu: 2.553 ± 0.295
0.426IleMet: 0.426 ± 0.14
1.277IleAsn: 1.277 ± 0.198
1.755IlePro: 1.755 ± 0.381
0.904IleGln: 0.904 ± 0.246
2.287IleArg: 2.287 ± 0.361
1.277IleSer: 1.277 ± 0.275
2.34IleThr: 2.34 ± 0.335
3.723IleVal: 3.723 ± 0.362
0.745IleTrp: 0.745 ± 0.213
0.532IleTyr: 0.532 ± 0.154
0.0IleXaa: 0.0 ± 0.0
Lys
4.255LysAla: 4.255 ± 0.398
0.426LysCys: 0.426 ± 0.156
1.223LysAsp: 1.223 ± 0.232
0.585LysGlu: 0.585 ± 0.153
0.957LysPhe: 0.957 ± 0.249
3.138LysGly: 3.138 ± 0.395
0.904LysHis: 0.904 ± 0.209
1.383LysIle: 1.383 ± 0.239
0.638LysLys: 0.638 ± 0.208
3.67LysLeu: 3.67 ± 0.395
0.851LysMet: 0.851 ± 0.23
0.745LysAsn: 0.745 ± 0.172
2.181LysPro: 2.181 ± 0.33
0.745LysGln: 0.745 ± 0.199
2.819LysArg: 2.819 ± 0.464
1.649LysSer: 1.649 ± 0.246
1.862LysThr: 1.862 ± 0.314
2.713LysVal: 2.713 ± 0.368
0.745LysTrp: 0.745 ± 0.166
0.798LysTyr: 0.798 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
12.18LeuAla: 12.18 ± 0.806
0.745LeuCys: 0.745 ± 0.193
7.925LeuAsp: 7.925 ± 0.667
2.234LeuGlu: 2.234 ± 0.386
2.074LeuPhe: 2.074 ± 0.367
6.915LeuGly: 6.915 ± 0.725
2.128LeuHis: 2.128 ± 0.413
3.564LeuIle: 3.564 ± 0.381
2.34LeuLys: 2.34 ± 0.381
6.117LeuLeu: 6.117 ± 0.554
1.808LeuMet: 1.808 ± 0.28
2.393LeuAsn: 2.393 ± 0.326
4.84LeuPro: 4.84 ± 0.521
2.447LeuGln: 2.447 ± 0.355
6.542LeuArg: 6.542 ± 0.503
6.064LeuSer: 6.064 ± 0.452
4.787LeuThr: 4.787 ± 0.486
5.585LeuVal: 5.585 ± 0.443
1.33LeuTrp: 1.33 ± 0.244
1.915LeuTyr: 1.915 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
2.872MetAla: 2.872 ± 0.374
0.106MetCys: 0.106 ± 0.079
0.957MetAsp: 0.957 ± 0.225
0.479MetGlu: 0.479 ± 0.158
0.638MetPhe: 0.638 ± 0.221
1.223MetGly: 1.223 ± 0.248
0.426MetHis: 0.426 ± 0.153
1.117MetIle: 1.117 ± 0.251
0.426MetLys: 0.426 ± 0.149
1.596MetLeu: 1.596 ± 0.288
0.426MetMet: 0.426 ± 0.151
0.585MetAsn: 0.585 ± 0.166
1.223MetPro: 1.223 ± 0.251
1.011MetGln: 1.011 ± 0.218
1.542MetArg: 1.542 ± 0.243
2.5MetSer: 2.5 ± 0.293
1.755MetThr: 1.755 ± 0.299
1.808MetVal: 1.808 ± 0.292
0.319MetTrp: 0.319 ± 0.114
0.479MetTyr: 0.479 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.404AsnAla: 3.404 ± 0.386
0.266AsnCys: 0.266 ± 0.11
1.383AsnAsp: 1.383 ± 0.288
1.33AsnGlu: 1.33 ± 0.273
0.851AsnPhe: 0.851 ± 0.275
4.361AsnGly: 4.361 ± 0.448
0.479AsnHis: 0.479 ± 0.146
1.064AsnIle: 1.064 ± 0.249
0.745AsnLys: 0.745 ± 0.166
2.34AsnLeu: 2.34 ± 0.352
0.585AsnMet: 0.585 ± 0.143
0.904AsnAsn: 0.904 ± 0.211
2.234AsnPro: 2.234 ± 0.325
0.585AsnGln: 0.585 ± 0.19
2.447AsnArg: 2.447 ± 0.376
0.745AsnSer: 0.745 ± 0.182
1.596AsnThr: 1.596 ± 0.287
2.234AsnVal: 2.234 ± 0.356
0.479AsnTrp: 0.479 ± 0.15
0.532AsnTyr: 0.532 ± 0.138
0.0AsnXaa: 0.0 ± 0.0
Pro
7.872ProAla: 7.872 ± 0.693
0.638ProCys: 0.638 ± 0.197
3.085ProAsp: 3.085 ± 0.403
4.627ProGlu: 4.627 ± 0.521
1.862ProPhe: 1.862 ± 0.287
5.638ProGly: 5.638 ± 0.457
1.383ProHis: 1.383 ± 0.265
2.234ProIle: 2.234 ± 0.242
1.915ProLys: 1.915 ± 0.333
4.042ProLeu: 4.042 ± 0.511
0.798ProMet: 0.798 ± 0.168
1.649ProAsn: 1.649 ± 0.296
2.979ProPro: 2.979 ± 0.485
1.542ProGln: 1.542 ± 0.341
3.245ProArg: 3.245 ± 0.455
3.085ProSer: 3.085 ± 0.557
3.51ProThr: 3.51 ± 0.513
5.053ProVal: 5.053 ± 0.562
1.277ProTrp: 1.277 ± 0.254
1.383ProTyr: 1.383 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
4.255GlnAla: 4.255 ± 0.51
0.319GlnCys: 0.319 ± 0.118
1.277GlnAsp: 1.277 ± 0.219
0.957GlnGlu: 0.957 ± 0.187
0.851GlnPhe: 0.851 ± 0.284
1.808GlnGly: 1.808 ± 0.285
1.223GlnHis: 1.223 ± 0.228
1.862GlnIle: 1.862 ± 0.301
0.851GlnLys: 0.851 ± 0.242
2.074GlnLeu: 2.074 ± 0.37
0.798GlnMet: 0.798 ± 0.2
0.691GlnAsn: 0.691 ± 0.192
2.447GlnPro: 2.447 ± 0.358
1.596GlnGln: 1.596 ± 0.314
2.393GlnArg: 2.393 ± 0.329
1.17GlnSer: 1.17 ± 0.2
2.074GlnThr: 2.074 ± 0.322
3.245GlnVal: 3.245 ± 0.466
0.745GlnTrp: 0.745 ± 0.21
0.957GlnTyr: 0.957 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
7.074ArgAla: 7.074 ± 0.771
1.383ArgCys: 1.383 ± 0.312
5.0ArgAsp: 5.0 ± 0.732
4.681ArgGlu: 4.681 ± 0.546
2.128ArgPhe: 2.128 ± 0.341
5.053ArgGly: 5.053 ± 0.499
1.755ArgHis: 1.755 ± 0.364
3.351ArgIle: 3.351 ± 0.484
3.564ArgLys: 3.564 ± 0.513
6.117ArgLeu: 6.117 ± 0.715
1.915ArgMet: 1.915 ± 0.273
2.128ArgAsn: 2.128 ± 0.415
3.404ArgPro: 3.404 ± 0.483
2.553ArgGln: 2.553 ± 0.313
5.904ArgArg: 5.904 ± 0.604
2.979ArgSer: 2.979 ± 0.354
3.617ArgThr: 3.617 ± 0.522
5.106ArgVal: 5.106 ± 0.465
1.649ArgTrp: 1.649 ± 0.311
1.755ArgTyr: 1.755 ± 0.301
0.0ArgXaa: 0.0 ± 0.0
Ser
6.383SerAla: 6.383 ± 0.763
0.266SerCys: 0.266 ± 0.133
3.085SerAsp: 3.085 ± 0.351
2.766SerGlu: 2.766 ± 0.384
1.436SerPhe: 1.436 ± 0.311
4.308SerGly: 4.308 ± 0.828
1.17SerHis: 1.17 ± 0.247
1.649SerIle: 1.649 ± 0.297
1.33SerLys: 1.33 ± 0.236
4.627SerLeu: 4.627 ± 0.495
1.702SerMet: 1.702 ± 0.306
1.117SerAsn: 1.117 ± 0.184
2.925SerPro: 2.925 ± 0.399
2.074SerGln: 2.074 ± 0.288
2.447SerArg: 2.447 ± 0.314
2.34SerSer: 2.34 ± 0.428
4.096SerThr: 4.096 ± 0.629
3.404SerVal: 3.404 ± 0.469
1.649SerTrp: 1.649 ± 0.324
1.383SerTyr: 1.383 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
7.021ThrAla: 7.021 ± 0.71
0.479ThrCys: 0.479 ± 0.182
3.404ThrAsp: 3.404 ± 0.479
3.457ThrGlu: 3.457 ± 0.404
2.287ThrPhe: 2.287 ± 0.273
5.638ThrGly: 5.638 ± 0.566
1.862ThrHis: 1.862 ± 0.405
3.032ThrIle: 3.032 ± 0.421
1.702ThrLys: 1.702 ± 0.321
3.776ThrLeu: 3.776 ± 0.484
0.745ThrMet: 0.745 ± 0.189
1.702ThrAsn: 1.702 ± 0.296
4.096ThrPro: 4.096 ± 0.538
1.542ThrGln: 1.542 ± 0.307
3.351ThrArg: 3.351 ± 0.462
2.659ThrSer: 2.659 ± 0.473
3.138ThrThr: 3.138 ± 0.489
5.159ThrVal: 5.159 ± 0.651
1.489ThrTrp: 1.489 ± 0.273
0.798ThrTyr: 0.798 ± 0.211
0.0ThrXaa: 0.0 ± 0.0
Val
10.372ValAla: 10.372 ± 0.919
0.957ValCys: 0.957 ± 0.262
5.585ValAsp: 5.585 ± 0.584
6.01ValGlu: 6.01 ± 0.781
1.489ValPhe: 1.489 ± 0.289
6.329ValGly: 6.329 ± 0.787
1.808ValHis: 1.808 ± 0.401
3.191ValIle: 3.191 ± 0.442
2.5ValLys: 2.5 ± 0.417
6.489ValLeu: 6.489 ± 0.545
1.117ValMet: 1.117 ± 0.248
2.287ValAsn: 2.287 ± 0.302
5.106ValPro: 5.106 ± 0.461
2.021ValGln: 2.021 ± 0.364
5.212ValArg: 5.212 ± 0.603
3.776ValSer: 3.776 ± 0.482
4.893ValThr: 4.893 ± 0.531
6.01ValVal: 6.01 ± 0.662
2.447ValTrp: 2.447 ± 0.308
1.596ValTyr: 1.596 ± 0.27
0.0ValXaa: 0.0 ± 0.0
Trp
2.128TrpAla: 2.128 ± 0.388
0.426TrpCys: 0.426 ± 0.144
0.957TrpAsp: 0.957 ± 0.229
0.532TrpGlu: 0.532 ± 0.175
0.691TrpPhe: 0.691 ± 0.192
1.596TrpGly: 1.596 ± 0.269
0.585TrpHis: 0.585 ± 0.179
0.638TrpIle: 0.638 ± 0.173
0.372TrpLys: 0.372 ± 0.138
3.032TrpLeu: 3.032 ± 0.422
0.266TrpMet: 0.266 ± 0.157
0.851TrpAsn: 0.851 ± 0.208
1.064TrpPro: 1.064 ± 0.234
1.33TrpGln: 1.33 ± 0.214
2.181TrpArg: 2.181 ± 0.372
1.277TrpSer: 1.277 ± 0.257
1.064TrpThr: 1.064 ± 0.262
1.489TrpVal: 1.489 ± 0.25
0.319TrpTrp: 0.319 ± 0.112
0.532TrpTyr: 0.532 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.138TyrAla: 3.138 ± 0.401
0.479TyrCys: 0.479 ± 0.155
1.702TyrAsp: 1.702 ± 0.312
1.649TyrGlu: 1.649 ± 0.283
0.638TyrPhe: 0.638 ± 0.19
2.5TyrGly: 2.5 ± 0.389
0.106TyrHis: 0.106 ± 0.074
0.532TyrIle: 0.532 ± 0.138
0.479TyrLys: 0.479 ± 0.161
1.808TyrLeu: 1.808 ± 0.274
0.479TyrMet: 0.479 ± 0.187
0.426TyrAsn: 0.426 ± 0.111
1.223TyrPro: 1.223 ± 0.277
0.798TyrGln: 0.798 ± 0.223
2.074TyrArg: 2.074 ± 0.345
1.33TyrSer: 1.33 ± 0.257
1.383TyrThr: 1.383 ± 0.254
1.862TyrVal: 1.862 ± 0.34
0.479TyrTrp: 0.479 ± 0.163
0.638TyrTyr: 0.638 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (18802 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski