Amino acid dipepetide frequency for Gordonia phage Ewald

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.424AlaAla: 19.424 ± 2.366
0.525AlaCys: 0.525 ± 0.2
9.825AlaAsp: 9.825 ± 0.834
6.975AlaGlu: 6.975 ± 0.898
2.175AlaPhe: 2.175 ± 0.433
11.624AlaGly: 11.624 ± 1.317
1.875AlaHis: 1.875 ± 0.455
4.8AlaIle: 4.8 ± 0.603
4.05AlaLys: 4.05 ± 0.551
9.675AlaLeu: 9.675 ± 1.037
3.375AlaMet: 3.375 ± 0.52
3.075AlaAsn: 3.075 ± 0.769
5.25AlaPro: 5.25 ± 0.987
4.35AlaGln: 4.35 ± 0.496
7.8AlaArg: 7.8 ± 0.892
5.85AlaSer: 5.85 ± 1.013
7.35AlaThr: 7.35 ± 0.785
7.125AlaVal: 7.125 ± 0.701
1.5AlaTrp: 1.5 ± 0.343
3.075AlaTyr: 3.075 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
0.675CysAla: 0.675 ± 0.258
0.15CysCys: 0.15 ± 0.143
0.825CysAsp: 0.825 ± 0.302
0.375CysGlu: 0.375 ± 0.181
0.0CysPhe: 0.0 ± 0.0
1.05CysGly: 1.05 ± 0.329
0.15CysHis: 0.15 ± 0.113
0.45CysIle: 0.45 ± 0.171
0.15CysLys: 0.15 ± 0.091
0.225CysLeu: 0.225 ± 0.122
0.075CysMet: 0.075 ± 0.073
0.075CysAsn: 0.075 ± 0.072
0.525CysPro: 0.525 ± 0.2
0.075CysGln: 0.075 ± 0.083
0.9CysArg: 0.9 ± 0.335
0.525CysSer: 0.525 ± 0.169
0.6CysThr: 0.6 ± 0.202
0.825CysVal: 0.825 ± 0.295
0.075CysTrp: 0.075 ± 0.084
0.075CysTyr: 0.075 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
8.7AspAla: 8.7 ± 0.703
0.525AspCys: 0.525 ± 0.211
4.575AspAsp: 4.575 ± 0.876
5.25AspGlu: 5.25 ± 0.589
1.425AspPhe: 1.425 ± 0.278
7.95AspGly: 7.95 ± 0.709
1.5AspHis: 1.5 ± 0.4
2.7AspIle: 2.7 ± 0.459
2.175AspLys: 2.175 ± 0.528
5.85AspLeu: 5.85 ± 0.759
1.425AspMet: 1.425 ± 0.316
1.8AspAsn: 1.8 ± 0.359
6.075AspPro: 6.075 ± 0.752
2.4AspGln: 2.4 ± 0.403
5.175AspArg: 5.175 ± 0.593
3.15AspSer: 3.15 ± 0.578
2.625AspThr: 2.625 ± 0.453
4.575AspVal: 4.575 ± 0.683
1.35AspTrp: 1.35 ± 0.469
0.9AspTyr: 0.9 ± 0.231
0.0AspXaa: 0.0 ± 0.0
Glu
6.375GluAla: 6.375 ± 0.68
0.6GluCys: 0.6 ± 0.24
2.7GluAsp: 2.7 ± 0.574
2.25GluGlu: 2.25 ± 0.452
2.025GluPhe: 2.025 ± 0.39
2.85GluGly: 2.85 ± 0.462
1.725GluHis: 1.725 ± 0.341
3.375GluIle: 3.375 ± 0.437
1.725GluLys: 1.725 ± 0.307
4.875GluLeu: 4.875 ± 0.437
1.875GluMet: 1.875 ± 0.318
2.475GluAsn: 2.475 ± 0.463
2.325GluPro: 2.325 ± 0.468
2.925GluGln: 2.925 ± 0.466
4.5GluArg: 4.5 ± 0.681
2.925GluSer: 2.925 ± 0.399
3.375GluThr: 3.375 ± 0.396
3.9GluVal: 3.9 ± 0.566
2.4GluTrp: 2.4 ± 0.496
1.35GluTyr: 1.35 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.311
0.225PheCys: 0.225 ± 0.207
2.25PheAsp: 2.25 ± 0.417
1.275PheGlu: 1.275 ± 0.296
0.825PhePhe: 0.825 ± 0.267
2.55PheGly: 2.55 ± 0.432
0.3PheHis: 0.3 ± 0.13
0.825PheIle: 0.825 ± 0.263
0.675PheLys: 0.675 ± 0.221
0.975PheLeu: 0.975 ± 0.241
0.375PheMet: 0.375 ± 0.164
1.05PheAsn: 1.05 ± 0.383
1.95PhePro: 1.95 ± 0.384
0.375PheGln: 0.375 ± 0.197
2.025PheArg: 2.025 ± 0.377
1.5PheSer: 1.5 ± 0.358
2.325PheThr: 2.325 ± 0.497
1.425PheVal: 1.425 ± 0.27
0.45PheTrp: 0.45 ± 0.154
0.675PheTyr: 0.675 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
9.825GlyAla: 9.825 ± 1.451
0.525GlyCys: 0.525 ± 0.243
6.825GlyAsp: 6.825 ± 0.576
4.8GlyGlu: 4.8 ± 0.66
2.325GlyPhe: 2.325 ± 0.468
9.975GlyGly: 9.975 ± 1.938
1.725GlyHis: 1.725 ± 0.334
3.9GlyIle: 3.9 ± 0.681
2.4GlyLys: 2.4 ± 0.346
7.425GlyLeu: 7.425 ± 0.8
2.025GlyMet: 2.025 ± 0.399
2.175GlyAsn: 2.175 ± 0.418
5.1GlyPro: 5.1 ± 0.68
4.2GlyGln: 4.2 ± 0.789
5.925GlyArg: 5.925 ± 0.695
5.025GlySer: 5.025 ± 0.633
5.775GlyThr: 5.775 ± 0.692
6.225GlyVal: 6.225 ± 0.925
2.175GlyTrp: 2.175 ± 0.362
1.725GlyTyr: 1.725 ± 0.441
0.0GlyXaa: 0.0 ± 0.0
His
1.8HisAla: 1.8 ± 0.304
0.225HisCys: 0.225 ± 0.126
1.425HisAsp: 1.425 ± 0.412
1.35HisGlu: 1.35 ± 0.252
0.3HisPhe: 0.3 ± 0.114
1.8HisGly: 1.8 ± 0.405
0.45HisHis: 0.45 ± 0.2
0.675HisIle: 0.675 ± 0.248
0.675HisLys: 0.675 ± 0.188
1.8HisLeu: 1.8 ± 0.363
0.45HisMet: 0.45 ± 0.182
0.45HisAsn: 0.45 ± 0.221
1.5HisPro: 1.5 ± 0.403
0.3HisGln: 0.3 ± 0.145
1.95HisArg: 1.95 ± 0.361
0.9HisSer: 0.9 ± 0.27
0.9HisThr: 0.9 ± 0.293
1.125HisVal: 1.125 ± 0.246
0.525HisTrp: 0.525 ± 0.246
0.45HisTyr: 0.45 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
5.475IleAla: 5.475 ± 0.825
0.375IleCys: 0.375 ± 0.167
3.225IleAsp: 3.225 ± 0.487
3.225IleGlu: 3.225 ± 0.527
0.75IlePhe: 0.75 ± 0.244
3.375IleGly: 3.375 ± 0.472
0.825IleHis: 0.825 ± 0.257
1.35IleIle: 1.35 ± 0.246
1.125IleLys: 1.125 ± 0.242
3.0IleLeu: 3.0 ± 0.486
0.45IleMet: 0.45 ± 0.172
1.35IleAsn: 1.35 ± 0.328
2.625IlePro: 2.625 ± 0.455
1.275IleGln: 1.275 ± 0.343
3.6IleArg: 3.6 ± 0.58
2.625IleSer: 2.625 ± 0.454
2.7IleThr: 2.7 ± 0.364
3.975IleVal: 3.975 ± 0.692
0.825IleTrp: 0.825 ± 0.308
0.975IleTyr: 0.975 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
4.125LysAla: 4.125 ± 0.676
0.15LysCys: 0.15 ± 0.108
1.2LysAsp: 1.2 ± 0.217
1.35LysGlu: 1.35 ± 0.391
0.975LysPhe: 0.975 ± 0.202
1.65LysGly: 1.65 ± 0.494
0.6LysHis: 0.6 ± 0.24
1.2LysIle: 1.2 ± 0.22
1.05LysLys: 1.05 ± 0.307
3.075LysLeu: 3.075 ± 0.57
0.6LysMet: 0.6 ± 0.237
0.675LysAsn: 0.675 ± 0.2
1.8LysPro: 1.8 ± 0.284
1.95LysGln: 1.95 ± 0.541
1.875LysArg: 1.875 ± 0.333
2.625LysSer: 2.625 ± 0.53
1.95LysThr: 1.95 ± 0.326
2.55LysVal: 2.55 ± 0.424
0.975LysTrp: 0.975 ± 0.382
0.975LysTyr: 0.975 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
10.199LeuAla: 10.199 ± 0.843
0.3LeuCys: 0.3 ± 0.177
4.95LeuAsp: 4.95 ± 0.633
4.125LeuGlu: 4.125 ± 0.465
1.8LeuPhe: 1.8 ± 0.348
6.3LeuGly: 6.3 ± 0.772
1.575LeuHis: 1.575 ± 0.364
3.3LeuIle: 3.3 ± 0.634
2.775LeuLys: 2.775 ± 0.498
5.925LeuLeu: 5.925 ± 0.538
1.65LeuMet: 1.65 ± 0.359
2.175LeuAsn: 2.175 ± 0.417
5.625LeuPro: 5.625 ± 0.721
2.4LeuGln: 2.4 ± 0.476
5.85LeuArg: 5.85 ± 0.553
4.35LeuSer: 4.35 ± 0.6
5.925LeuThr: 5.925 ± 0.584
5.475LeuVal: 5.475 ± 0.732
1.125LeuTrp: 1.125 ± 0.29
1.65LeuTyr: 1.65 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
3.0MetAla: 3.0 ± 0.514
0.225MetCys: 0.225 ± 0.115
1.575MetAsp: 1.575 ± 0.388
0.675MetGlu: 0.675 ± 0.293
0.3MetPhe: 0.3 ± 0.159
1.5MetGly: 1.5 ± 0.412
0.525MetHis: 0.525 ± 0.181
0.975MetIle: 0.975 ± 0.318
0.525MetLys: 0.525 ± 0.21
1.725MetLeu: 1.725 ± 0.383
0.375MetMet: 0.375 ± 0.178
0.825MetAsn: 0.825 ± 0.245
1.275MetPro: 1.275 ± 0.335
1.05MetGln: 1.05 ± 0.357
1.8MetArg: 1.8 ± 0.448
1.875MetSer: 1.875 ± 0.418
2.4MetThr: 2.4 ± 0.479
1.35MetVal: 1.35 ± 0.327
0.6MetTrp: 0.6 ± 0.19
0.3MetTyr: 0.3 ± 0.206
0.0MetXaa: 0.0 ± 0.0
Asn
3.6AsnAla: 3.6 ± 0.552
0.225AsnCys: 0.225 ± 0.127
1.575AsnAsp: 1.575 ± 0.343
1.2AsnGlu: 1.2 ± 0.3
0.6AsnPhe: 0.6 ± 0.199
3.75AsnGly: 3.75 ± 0.652
0.6AsnHis: 0.6 ± 0.215
1.2AsnIle: 1.2 ± 0.35
0.675AsnLys: 0.675 ± 0.235
1.5AsnLeu: 1.5 ± 0.28
0.6AsnMet: 0.6 ± 0.189
0.9AsnAsn: 0.9 ± 0.274
2.175AsnPro: 2.175 ± 0.401
1.2AsnGln: 1.2 ± 0.28
2.55AsnArg: 2.55 ± 0.517
1.725AsnSer: 1.725 ± 0.354
2.7AsnThr: 2.7 ± 0.543
1.35AsnVal: 1.35 ± 0.257
0.525AsnTrp: 0.525 ± 0.204
0.225AsnTyr: 0.225 ± 0.131
0.0AsnXaa: 0.0 ± 0.0
Pro
7.575ProAla: 7.575 ± 0.978
0.375ProCys: 0.375 ± 0.174
5.775ProAsp: 5.775 ± 0.679
3.975ProGlu: 3.975 ± 0.634
1.425ProPhe: 1.425 ± 0.278
5.55ProGly: 5.55 ± 0.53
1.125ProHis: 1.125 ± 0.346
2.85ProIle: 2.85 ± 0.426
1.575ProLys: 1.575 ± 0.315
4.5ProLeu: 4.5 ± 0.643
1.425ProMet: 1.425 ± 0.379
2.025ProAsn: 2.025 ± 0.396
4.5ProPro: 4.5 ± 0.885
2.025ProGln: 2.025 ± 0.396
3.675ProArg: 3.675 ± 0.535
3.375ProSer: 3.375 ± 0.848
3.45ProThr: 3.45 ± 0.425
4.275ProVal: 4.275 ± 0.633
1.275ProTrp: 1.275 ± 0.351
1.65ProTyr: 1.65 ± 0.394
0.0ProXaa: 0.0 ± 0.0
Gln
3.825GlnAla: 3.825 ± 0.845
0.15GlnCys: 0.15 ± 0.104
1.35GlnAsp: 1.35 ± 0.267
1.35GlnGlu: 1.35 ± 0.321
1.275GlnPhe: 1.275 ± 0.34
2.4GlnGly: 2.4 ± 0.364
0.6GlnHis: 0.6 ± 0.208
2.55GlnIle: 2.55 ± 0.395
1.2GlnLys: 1.2 ± 0.307
3.525GlnLeu: 3.525 ± 0.489
1.125GlnMet: 1.125 ± 0.327
1.05GlnAsn: 1.05 ± 0.239
2.55GlnPro: 2.55 ± 0.51
2.325GlnGln: 2.325 ± 0.513
3.15GlnArg: 3.15 ± 0.521
1.725GlnSer: 1.725 ± 0.43
2.625GlnThr: 2.625 ± 0.394
2.7GlnVal: 2.7 ± 0.4
0.6GlnTrp: 0.6 ± 0.183
0.825GlnTyr: 0.825 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
6.75ArgAla: 6.75 ± 0.806
0.6ArgCys: 0.6 ± 0.229
4.725ArgAsp: 4.725 ± 0.595
4.05ArgGlu: 4.05 ± 0.748
2.25ArgPhe: 2.25 ± 0.445
5.925ArgGly: 5.925 ± 0.664
2.175ArgHis: 2.175 ± 0.419
3.6ArgIle: 3.6 ± 0.556
2.925ArgLys: 2.925 ± 0.367
6.225ArgLeu: 6.225 ± 0.744
2.4ArgMet: 2.4 ± 0.406
2.175ArgAsn: 2.175 ± 0.461
3.675ArgPro: 3.675 ± 0.673
2.625ArgGln: 2.625 ± 0.335
8.4ArgArg: 8.4 ± 1.089
4.2ArgSer: 4.2 ± 0.53
3.825ArgThr: 3.825 ± 0.517
5.25ArgVal: 5.25 ± 0.714
1.95ArgTrp: 1.95 ± 0.384
1.875ArgTyr: 1.875 ± 0.426
0.0ArgXaa: 0.0 ± 0.0
Ser
6.15SerAla: 6.15 ± 0.818
0.3SerCys: 0.3 ± 0.141
4.2SerAsp: 4.2 ± 0.581
2.775SerGlu: 2.775 ± 0.43
1.35SerPhe: 1.35 ± 0.3
5.325SerGly: 5.325 ± 0.773
0.075SerHis: 0.075 ± 0.071
2.55SerIle: 2.55 ± 0.417
2.325SerLys: 2.325 ± 0.394
4.05SerLeu: 4.05 ± 0.536
1.5SerMet: 1.5 ± 0.459
1.725SerAsn: 1.725 ± 0.37
3.6SerPro: 3.6 ± 0.547
1.875SerGln: 1.875 ± 0.366
3.525SerArg: 3.525 ± 0.52
2.475SerSer: 2.475 ± 0.535
4.425SerThr: 4.425 ± 0.423
4.95SerVal: 4.95 ± 0.574
0.75SerTrp: 0.75 ± 0.218
0.825SerTyr: 0.825 ± 0.221
0.0SerXaa: 0.0 ± 0.0
Thr
8.4ThrAla: 8.4 ± 1.062
0.45ThrCys: 0.45 ± 0.24
4.8ThrAsp: 4.8 ± 0.525
3.9ThrGlu: 3.9 ± 0.521
1.425ThrPhe: 1.425 ± 0.445
5.85ThrGly: 5.85 ± 0.835
1.125ThrHis: 1.125 ± 0.222
2.55ThrIle: 2.55 ± 0.447
1.875ThrLys: 1.875 ± 0.412
4.125ThrLeu: 4.125 ± 0.522
0.975ThrMet: 0.975 ± 0.294
1.875ThrAsn: 1.875 ± 0.317
5.325ThrPro: 5.325 ± 0.682
1.875ThrGln: 1.875 ± 0.35
3.825ThrArg: 3.825 ± 0.529
3.675ThrSer: 3.675 ± 0.459
5.1ThrThr: 5.1 ± 0.824
5.025ThrVal: 5.025 ± 0.608
1.275ThrTrp: 1.275 ± 0.371
1.05ThrTyr: 1.05 ± 0.299
0.0ThrXaa: 0.0 ± 0.0
Val
7.275ValAla: 7.275 ± 0.894
0.9ValCys: 0.9 ± 0.287
5.775ValAsp: 5.775 ± 0.781
5.1ValGlu: 5.1 ± 0.658
1.875ValPhe: 1.875 ± 0.411
6.6ValGly: 6.6 ± 0.655
1.35ValHis: 1.35 ± 0.378
2.4ValIle: 2.4 ± 0.48
2.175ValLys: 2.175 ± 0.349
5.625ValLeu: 5.625 ± 0.678
1.425ValMet: 1.425 ± 0.259
1.575ValAsn: 1.575 ± 0.347
5.1ValPro: 5.1 ± 0.644
1.725ValGln: 1.725 ± 0.49
5.775ValArg: 5.775 ± 0.682
3.9ValSer: 3.9 ± 0.664
4.425ValThr: 4.425 ± 0.534
5.7ValVal: 5.7 ± 0.75
1.8ValTrp: 1.8 ± 0.365
1.65ValTyr: 1.65 ± 0.41
0.0ValXaa: 0.0 ± 0.0
Trp
1.725TrpAla: 1.725 ± 0.336
0.6TrpCys: 0.6 ± 0.207
1.05TrpAsp: 1.05 ± 0.368
1.05TrpGlu: 1.05 ± 0.368
0.825TrpPhe: 0.825 ± 0.316
1.725TrpGly: 1.725 ± 0.319
0.45TrpHis: 0.45 ± 0.221
0.825TrpIle: 0.825 ± 0.252
0.45TrpLys: 0.45 ± 0.187
1.65TrpLeu: 1.65 ± 0.306
0.525TrpMet: 0.525 ± 0.153
0.825TrpAsn: 0.825 ± 0.277
0.975TrpPro: 0.975 ± 0.316
1.275TrpGln: 1.275 ± 0.242
1.725TrpArg: 1.725 ± 0.389
1.35TrpSer: 1.35 ± 0.379
1.05TrpThr: 1.05 ± 0.325
2.1TrpVal: 2.1 ± 0.434
0.6TrpTrp: 0.6 ± 0.221
0.375TrpTyr: 0.375 ± 0.195
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.4TyrAla: 2.4 ± 0.397
0.375TyrCys: 0.375 ± 0.178
1.5TyrAsp: 1.5 ± 0.357
1.5TyrGlu: 1.5 ± 0.403
0.6TyrPhe: 0.6 ± 0.226
2.325TyrGly: 2.325 ± 0.506
0.3TyrHis: 0.3 ± 0.182
1.05TyrIle: 1.05 ± 0.271
0.825TyrLys: 0.825 ± 0.24
1.725TyrLeu: 1.725 ± 0.33
0.15TyrMet: 0.15 ± 0.121
0.6TyrAsn: 0.6 ± 0.182
0.45TyrPro: 0.45 ± 0.174
0.675TyrGln: 0.675 ± 0.207
1.575TyrArg: 1.575 ± 0.402
0.975TyrSer: 0.975 ± 0.261
0.975TyrThr: 0.975 ± 0.311
2.25TyrVal: 2.25 ± 0.446
0.375TyrTrp: 0.375 ± 0.186
0.45TyrTyr: 0.45 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13335 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski