Lake Sarah-associated circular virus-10

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQK3|A0A140AQK3_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-10 OX=1685736 PE=3 SV=1
MM1 pKa = 7.75TDD3 pKa = 2.65TRR5 pKa = 11.84SRR7 pKa = 11.84DD8 pKa = 2.85WCFTLNNYY16 pKa = 7.52TEE18 pKa = 4.18EE19 pKa = 4.64EE20 pKa = 4.28YY21 pKa = 11.16DD22 pKa = 3.98VIHH25 pKa = 6.51SLSLSQYY32 pKa = 9.74KK33 pKa = 10.27YY34 pKa = 9.25IVVGKK39 pKa = 9.62EE40 pKa = 3.62VGEE43 pKa = 4.34SGTNHH48 pKa = 5.71LQGYY52 pKa = 9.67IYY54 pKa = 10.19FVNAKK59 pKa = 10.21SMSAVKK65 pKa = 10.61KK66 pKa = 9.48MISKK70 pKa = 10.01RR71 pKa = 11.84CHH73 pKa = 6.31LEE75 pKa = 3.77SAKK78 pKa = 10.57GSPLQAATYY87 pKa = 9.6CKK89 pKa = 10.16KK90 pKa = 10.83DD91 pKa = 3.37NNDD94 pKa = 3.24YY95 pKa = 11.12YY96 pKa = 11.17EE97 pKa = 4.43NGEE100 pKa = 4.14LPVIQGKK107 pKa = 7.81RR108 pKa = 11.84TDD110 pKa = 3.66LDD112 pKa = 4.02EE113 pKa = 5.28IRR115 pKa = 11.84DD116 pKa = 3.76ILKK119 pKa = 8.42QTNKK123 pKa = 9.47MSDD126 pKa = 3.09VVMVAKK132 pKa = 10.41SYY134 pKa = 11.07QSVKK138 pKa = 9.87MAEE141 pKa = 4.24QILKK145 pKa = 8.97YY146 pKa = 10.0HH147 pKa = 6.79EE148 pKa = 4.48KK149 pKa = 10.4PRR151 pKa = 11.84MEE153 pKa = 4.1KK154 pKa = 10.25PYY156 pKa = 10.51VEE158 pKa = 4.59WYY160 pKa = 9.99YY161 pKa = 11.56GPTGTGKK168 pKa = 10.18SKK170 pKa = 10.63KK171 pKa = 9.86AYY173 pKa = 8.7EE174 pKa = 4.13VLSDD178 pKa = 3.52EE179 pKa = 5.83CYY181 pKa = 9.81TCLSTGKK188 pKa = 9.52WFDD191 pKa = 3.95GYY193 pKa = 10.82DD194 pKa = 2.99AHH196 pKa = 7.74KK197 pKa = 10.91NVLIDD202 pKa = 4.76DD203 pKa = 3.82MRR205 pKa = 11.84KK206 pKa = 10.49DD207 pKa = 3.54FMKK210 pKa = 10.43FHH212 pKa = 6.83EE213 pKa = 4.91LLRR216 pKa = 11.84LLDD219 pKa = 4.22RR220 pKa = 11.84YY221 pKa = 11.07AMRR224 pKa = 11.84VEE226 pKa = 4.5CKK228 pKa = 10.11GGTRR232 pKa = 11.84QFVASHH238 pKa = 6.9IIITSCYY245 pKa = 8.27HH246 pKa = 6.1PKK248 pKa = 11.19DD249 pKa = 3.43MFEE252 pKa = 4.03TRR254 pKa = 11.84EE255 pKa = 4.67DD256 pKa = 3.29IQQLLRR262 pKa = 11.84RR263 pKa = 11.84IDD265 pKa = 3.7KK266 pKa = 10.63IEE268 pKa = 3.96NFEE271 pKa = 4.03

Molecular weight: 31.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQK3|A0A140AQK3_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-10 OX=1685736 PE=3 SV=1
MM1 pKa = 7.59AKK3 pKa = 9.96FVKK6 pKa = 10.17KK7 pKa = 10.21PYY9 pKa = 9.93RR10 pKa = 11.84KK11 pKa = 8.78VRR13 pKa = 11.84KK14 pKa = 8.7YY15 pKa = 10.87AKK17 pKa = 10.24KK18 pKa = 9.7PVKK21 pKa = 10.64KK22 pKa = 9.97MMKK25 pKa = 8.35TSKK28 pKa = 10.73SFVKK32 pKa = 10.17KK33 pKa = 8.31VQKK36 pKa = 10.25IIHH39 pKa = 6.8KK40 pKa = 10.14DD41 pKa = 3.25VEE43 pKa = 4.58TKK45 pKa = 10.79VVVFNSNATAFNQQINSTGDD65 pKa = 3.63CLRR68 pKa = 11.84LLPDD72 pKa = 3.58IVNGTSEE79 pKa = 3.91NTKK82 pKa = 9.77IGNIIQLQSLNIRR95 pKa = 11.84GVLTFALSQTASQNVRR111 pKa = 11.84IGVRR115 pKa = 11.84MLILRR120 pKa = 11.84AKK122 pKa = 10.34RR123 pKa = 11.84FNDD126 pKa = 3.19WNQSATDD133 pKa = 3.92FATNYY138 pKa = 7.65TKK140 pKa = 10.83LLEE143 pKa = 4.65GSTSGFDD150 pKa = 3.44GSVAAFNTPVNHH162 pKa = 7.72DD163 pKa = 4.07YY164 pKa = 11.19FSVVADD170 pKa = 3.26KK171 pKa = 10.78RR172 pKa = 11.84FYY174 pKa = 10.07MSQSVILASGPTINTNEE191 pKa = 3.88TTKK194 pKa = 10.69FINFSVPYY202 pKa = 9.66SRR204 pKa = 11.84RR205 pKa = 11.84KK206 pKa = 8.51LTYY209 pKa = 9.87DD210 pKa = 3.0QDD212 pKa = 4.26FSGLAPTNYY221 pKa = 9.49PYY223 pKa = 11.28FMVLGYY229 pKa = 10.61SKK231 pKa = 11.24LDD233 pKa = 3.36GSVADD238 pKa = 4.31GTGTTYY244 pKa = 10.12LTFQYY249 pKa = 9.98TATAKK254 pKa = 10.91FEE256 pKa = 4.38DD257 pKa = 3.92AA258 pKa = 5.0

Molecular weight: 28.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

529

258

271

264.5

30.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.482 ± 0.967

1.512 ± 0.728

6.049 ± 0.654

4.915 ± 1.927

4.726 ± 1.206

5.293 ± 0.086

1.89 ± 0.722

5.293 ± 0.165

9.641 ± 0.219

6.805 ± 0.14

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.214 ± 0.575

5.293 ± 1.09

2.647 ± 0.294

3.97 ± 0.19

4.726 ± 0.299

7.372 ± 0.496

7.561 ± 1.377

6.994 ± 0.741

0.756 ± 0.239

5.86 ± 0.782
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski