Streptomyces phage Dubu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DEV9|A0A514DEV9_9CAUD Uncharacterized protein OS=Streptomyces phage Dubu OX=2591226 GN=41 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 5.47LEE5 pKa = 4.43GLDD8 pKa = 3.99PNHH11 pKa = 6.14VCGDD15 pKa = 3.83GCQPYY20 pKa = 9.75VPLIFRR26 pKa = 11.84DD27 pKa = 3.8LEE29 pKa = 4.13DD30 pKa = 5.13LEE32 pKa = 4.71EE33 pKa = 4.84SDD35 pKa = 6.03HH36 pKa = 7.99IDD38 pKa = 3.53ALYY41 pKa = 10.94DD42 pKa = 3.27VLNRR46 pKa = 11.84TRR48 pKa = 11.84EE49 pKa = 3.99HH50 pKa = 6.31GALLFLALNRR60 pKa = 11.84VAPTYY65 pKa = 10.2RR66 pKa = 11.84LPARR70 pKa = 11.84TAALLLDD77 pKa = 4.33LDD79 pKa = 4.27EE80 pKa = 6.8AEE82 pKa = 4.87LDD84 pKa = 3.97HH85 pKa = 7.2EE86 pKa = 5.62LGHH89 pKa = 5.87QPNYY93 pKa = 11.19ALGDD97 pKa = 3.65VVAFAVEE104 pKa = 4.25SQGTDD109 pKa = 3.36GYY111 pKa = 10.94VGKK114 pKa = 10.65AMEE117 pKa = 4.3ALFRR121 pKa = 11.84LEE123 pKa = 5.45LGLADD128 pKa = 5.42SDD130 pKa = 5.2DD131 pKa = 4.34EE132 pKa = 4.62EE133 pKa = 6.61DD134 pKa = 5.82DD135 pKa = 4.13EE136 pKa = 6.06EE137 pKa = 5.09EE138 pKa = 4.47DD139 pKa = 3.77

Molecular weight: 15.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DES7|A0A514DES7_9CAUD Tape measure protein OS=Streptomyces phage Dubu OX=2591226 GN=15 PE=4 SV=1
MM1 pKa = 7.45AWSLTARR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.21QEE12 pKa = 3.12KK13 pKa = 10.26RR14 pKa = 11.84AALAEE19 pKa = 4.06ATARR23 pKa = 11.84AVLAEE28 pKa = 4.0WSKK31 pKa = 10.88VRR33 pKa = 11.84PEE35 pKa = 3.82SVARR39 pKa = 11.84DD40 pKa = 3.42WAILLPQVTALVQRR54 pKa = 11.84GQLHH58 pKa = 5.88AAEE61 pKa = 5.26GSHH64 pKa = 4.68TFMRR68 pKa = 11.84EE69 pKa = 3.64LLGPPSRR76 pKa = 11.84EE77 pKa = 3.86TPQIDD82 pKa = 3.38PAQFATQAPDD92 pKa = 3.18GRR94 pKa = 11.84DD95 pKa = 3.29LMGVLARR102 pKa = 11.84SAPAAISGQRR112 pKa = 11.84RR113 pKa = 11.84GLSARR118 pKa = 11.84AAMARR123 pKa = 11.84GAAFLDD129 pKa = 3.69MVVRR133 pKa = 11.84TVVADD138 pKa = 3.66TGRR141 pKa = 11.84QADD144 pKa = 3.75QAAMVANKK152 pKa = 9.81GVRR155 pKa = 11.84AYY157 pKa = 10.84VRR159 pKa = 11.84VVEE162 pKa = 5.31LPACSRR168 pKa = 11.84CIILAGRR175 pKa = 11.84EE176 pKa = 4.04YY177 pKa = 10.79GVSSGFLRR185 pKa = 11.84HH186 pKa = 5.98PRR188 pKa = 11.84CDD190 pKa = 3.24CTMEE194 pKa = 4.05PVTRR198 pKa = 11.84RR199 pKa = 11.84NNPTPLDD206 pKa = 3.88AEE208 pKa = 4.54DD209 pKa = 4.93LFEE212 pKa = 5.1SMSPEE217 pKa = 3.87LRR219 pKa = 11.84RR220 pKa = 11.84KK221 pKa = 7.98TFGEE225 pKa = 4.21AGAKK229 pKa = 9.9AIQDD233 pKa = 3.61GARR236 pKa = 11.84ISTVVNARR244 pKa = 11.84KK245 pKa = 10.45SMDD248 pKa = 3.13RR249 pKa = 11.84VQMFGKK255 pKa = 8.16TVQVTYY261 pKa = 9.96TGTGSRR267 pKa = 11.84RR268 pKa = 11.84KK269 pKa = 9.57KK270 pKa = 10.17RR271 pKa = 11.84PPRR274 pKa = 11.84LMPEE278 pKa = 4.27EE279 pKa = 4.5IYY281 pKa = 10.86RR282 pKa = 11.84LAGDD286 pKa = 4.64DD287 pKa = 3.5RR288 pKa = 11.84DD289 pKa = 3.53HH290 pKa = 8.02AIRR293 pKa = 11.84LLYY296 pKa = 10.29KK297 pKa = 10.46NGYY300 pKa = 8.67LRR302 pKa = 4.69

Molecular weight: 33.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12107

44

1273

257.6

28.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.646 ± 0.513

0.743 ± 0.138

6.699 ± 0.247

5.955 ± 0.48

3.081 ± 0.2

8.185 ± 0.405

1.677 ± 0.166

3.304 ± 0.243

3.651 ± 0.32

8.524 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.205 ± 0.162

2.354 ± 0.263

5.856 ± 0.397

3.436 ± 0.195

7.599 ± 0.384

5.575 ± 0.315

6.583 ± 0.347

7.731 ± 0.273

1.842 ± 0.177

2.354 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski