Actinomadura rudentiformis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H9YPK2|A0A6H9YPK2_9ACTN Methyltransferase domain-containing protein OS=Actinomadura rudentiformis OX=359158 GN=F8566_35085 PE=4 SV=1
MM1 pKa = 7.48TLQEE5 pKa = 5.35LIGQLTEE12 pKa = 4.0ADD14 pKa = 3.8LAEE17 pKa = 4.5TDD19 pKa = 4.42DD20 pKa = 4.82DD21 pKa = 4.17LTVFASEE28 pKa = 4.37PWTADD33 pKa = 2.59SDD35 pKa = 3.96ATATPNPDD43 pKa = 4.34DD44 pKa = 3.95IARR47 pKa = 11.84PDD49 pKa = 3.73PQGRR53 pKa = 11.84TYY55 pKa = 11.09LLEE58 pKa = 4.86ISLIHH63 pKa = 7.15DD64 pKa = 3.8VLEE67 pKa = 4.32TWSAHH72 pKa = 5.42HH73 pKa = 6.76AGARR77 pKa = 11.84PSPQQACEE85 pKa = 3.62AVIYY89 pKa = 9.82YY90 pKa = 10.3AEE92 pKa = 4.67HH93 pKa = 7.0DD94 pKa = 4.36AYY96 pKa = 10.95LIPDD100 pKa = 4.6DD101 pKa = 4.69NPAA104 pKa = 3.92

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H9YSD7|A0A6H9YSD7_9ACTN TlpA family protein disulfide reductase OS=Actinomadura rudentiformis OX=359158 GN=F8566_07090 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLSARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.18GRR40 pKa = 11.84SRR42 pKa = 11.84IAVV45 pKa = 3.34

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9446

0

9446

3027437

29

5158

320.5

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.15 ± 0.032

0.783 ± 0.007

5.938 ± 0.019

5.697 ± 0.027

2.857 ± 0.012

9.286 ± 0.027

2.224 ± 0.011

3.747 ± 0.016

2.268 ± 0.022

10.293 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.92 ± 0.01

1.9 ± 0.015

6.091 ± 0.025

2.821 ± 0.013

8.198 ± 0.028

5.007 ± 0.018

5.9 ± 0.023

8.303 ± 0.022

1.561 ± 0.011

2.057 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski