Achromatium sp. WMS1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Achromatium; unclassified Achromatium

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M1J3N3|A0A0M1J3N3_9GAMM Uncharacterized protein (Fragment) OS=Achromatium sp. WMS1 OX=1604834 GN=TI03_07095 PE=4 SV=1
RR1 pKa = 8.48VVVLATDD8 pKa = 3.71YY9 pKa = 11.41DD10 pKa = 3.72FHH12 pKa = 7.44QAGDD16 pKa = 3.86HH17 pKa = 6.46SSVMPNNGDD26 pKa = 3.52TVLDD30 pKa = 4.09GDD32 pKa = 4.27PPGTGEE38 pKa = 5.23DD39 pKa = 4.59YY40 pKa = 8.84PTIDD44 pKa = 3.24QVKK47 pKa = 9.98DD48 pKa = 3.34ALVEE52 pKa = 4.04ANIYY56 pKa = 10.09PIFAVTYY63 pKa = 8.57GVQSSYY69 pKa = 11.84DD70 pKa = 3.51DD71 pKa = 4.14LVNQLGRR78 pKa = 11.84GDD80 pKa = 3.98TVEE83 pKa = 4.58LSSDD87 pKa = 3.06SSNIVSSIQTGLEE100 pKa = 3.71NYY102 pKa = 9.84KK103 pKa = 10.72ADD105 pKa = 4.53FIEE108 pKa = 4.5NVISTPYY115 pKa = 11.01DD116 pKa = 3.22DD117 pKa = 4.55TLTGNSLDD125 pKa = 3.65NRR127 pKa = 11.84IEE129 pKa = 4.23GGAGEE134 pKa = 4.6DD135 pKa = 3.64TLKK138 pKa = 11.29GLGGDD143 pKa = 3.76DD144 pKa = 3.75TLVGGDD150 pKa = 4.19DD151 pKa = 4.57ADD153 pKa = 3.79TAIYY157 pKa = 9.86RR158 pKa = 11.84GVYY161 pKa = 8.47STVPGEE167 pKa = 3.95TSEE170 pKa = 4.65YY171 pKa = 9.59EE172 pKa = 4.15VTRR175 pKa = 11.84TRR177 pKa = 11.84LDD179 pKa = 3.31VTVKK183 pKa = 10.85DD184 pKa = 4.1LVDD187 pKa = 3.73NRR189 pKa = 11.84DD190 pKa = 3.69DD191 pKa = 3.94TDD193 pKa = 3.47TLTDD197 pKa = 3.31IEE199 pKa = 4.55TLQFANGSIDD209 pKa = 3.73VSSLPVEE216 pKa = 4.4DD217 pKa = 4.91PSINFYY223 pKa = 10.2WSARR227 pKa = 11.84TIASIINNCYY237 pKa = 10.08QVMAFNWMNAICYY250 pKa = 8.6AAA252 pKa = 4.6

Molecular weight: 27.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M1J9Z0|A0A0M1J9Z0_9GAMM Nitric oxide reductase OS=Achromatium sp. WMS1 OX=1604834 GN=TI03_01640 PE=4 SV=1
MM1 pKa = 7.69PSFWQLRR8 pKa = 11.84CASYY12 pKa = 10.28IVKK15 pKa = 10.45AGGIIAYY22 pKa = 6.58PTEE25 pKa = 3.95AVFGLGCDD33 pKa = 4.09PYY35 pKa = 11.21NQRR38 pKa = 11.84ALTKK42 pKa = 10.04ILALKK47 pKa = 9.62GRR49 pKa = 11.84PINKK53 pKa = 9.4GVILIAADD61 pKa = 4.24FNQIIPFIAPLSNLPAARR79 pKa = 11.84ITQIKK84 pKa = 9.71ASWPGPVTWVLPARR98 pKa = 11.84NDD100 pKa = 3.22IPHH103 pKa = 7.28WLTGGRR109 pKa = 11.84HH110 pKa = 5.56TIAVRR115 pKa = 11.84ITAHH119 pKa = 6.53PSAVALCRR127 pKa = 11.84ICKK130 pKa = 7.87TALVSTSANSTNHH143 pKa = 5.88LPLRR147 pKa = 11.84NSLQLRR153 pKa = 11.84RR154 pKa = 11.84WLQRR158 pKa = 11.84LPGNNEE164 pKa = 3.18NHH166 pKa = 6.53INYY169 pKa = 9.32ILPGYY174 pKa = 9.16CGPTSRR180 pKa = 11.84PSRR183 pKa = 11.84IIDD186 pKa = 3.45ATSGLTLRR194 pKa = 11.84HH195 pKa = 5.73

Molecular weight: 21.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1173

0

1173

269738

28

1634

230.0

25.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.219 ± 0.087

1.096 ± 0.032

5.133 ± 0.079

5.458 ± 0.068

3.786 ± 0.044

6.473 ± 0.107

2.473 ± 0.047

7.596 ± 0.071

5.055 ± 0.07

10.742 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.038

4.784 ± 0.068

4.373 ± 0.057

4.91 ± 0.085

5.087 ± 0.066

5.69 ± 0.056

6.282 ± 0.074

6.185 ± 0.07

1.315 ± 0.031

3.097 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski