Amino acid dipepetide frequency for Streptococcus phage Javan394

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.201AlaAla: 4.201 ± 1.415
0.525AlaCys: 0.525 ± 0.193
3.501AlaAsp: 3.501 ± 0.649
5.076AlaGlu: 5.076 ± 0.914
3.413AlaPhe: 3.413 ± 0.978
4.113AlaGly: 4.113 ± 1.398
0.875AlaHis: 0.875 ± 0.289
5.514AlaIle: 5.514 ± 1.265
5.426AlaLys: 5.426 ± 0.862
6.914AlaLeu: 6.914 ± 1.261
2.363AlaMet: 2.363 ± 0.594
2.976AlaAsn: 2.976 ± 0.455
1.4AlaPro: 1.4 ± 0.27
3.063AlaGln: 3.063 ± 0.584
2.713AlaArg: 2.713 ± 0.586
3.851AlaSer: 3.851 ± 0.98
4.026AlaThr: 4.026 ± 0.821
4.639AlaVal: 4.639 ± 1.029
0.175AlaTrp: 0.175 ± 0.111
2.013AlaTyr: 2.013 ± 0.344
0.0AlaXaa: 0.0 ± 0.0
Cys
0.263CysAla: 0.263 ± 0.121
0.088CysCys: 0.088 ± 0.086
0.525CysAsp: 0.525 ± 0.167
0.35CysGlu: 0.35 ± 0.151
0.35CysPhe: 0.35 ± 0.189
0.613CysGly: 0.613 ± 0.289
0.0CysHis: 0.0 ± 0.0
0.263CysIle: 0.263 ± 0.143
0.175CysLys: 0.175 ± 0.127
0.525CysLeu: 0.525 ± 0.181
0.175CysMet: 0.175 ± 0.118
0.35CysAsn: 0.35 ± 0.167
0.35CysPro: 0.35 ± 0.19
0.263CysGln: 0.263 ± 0.163
0.0CysArg: 0.0 ± 0.0
0.175CysSer: 0.175 ± 0.1
0.175CysThr: 0.175 ± 0.125
0.263CysVal: 0.263 ± 0.174
0.088CysTrp: 0.088 ± 0.097
0.175CysTyr: 0.175 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
4.551AspAla: 4.551 ± 0.74
0.35AspCys: 0.35 ± 0.161
5.776AspAsp: 5.776 ± 1.038
5.076AspGlu: 5.076 ± 0.697
3.238AspPhe: 3.238 ± 0.619
6.301AspGly: 6.301 ± 0.856
0.613AspHis: 0.613 ± 0.234
4.814AspIle: 4.814 ± 0.763
5.601AspLys: 5.601 ± 0.864
6.389AspLeu: 6.389 ± 0.789
1.925AspMet: 1.925 ± 0.372
4.639AspAsn: 4.639 ± 0.688
0.963AspPro: 0.963 ± 0.28
1.925AspGln: 1.925 ± 0.266
2.188AspArg: 2.188 ± 0.526
4.026AspSer: 4.026 ± 0.703
3.676AspThr: 3.676 ± 0.712
4.288AspVal: 4.288 ± 0.658
0.963AspTrp: 0.963 ± 0.237
3.151AspTyr: 3.151 ± 0.598
0.0AspXaa: 0.0 ± 0.0
Glu
2.626GluAla: 2.626 ± 0.469
0.35GluCys: 0.35 ± 0.186
4.376GluAsp: 4.376 ± 0.823
4.201GluGlu: 4.201 ± 0.842
3.676GluPhe: 3.676 ± 0.531
2.363GluGly: 2.363 ± 0.293
0.788GluHis: 0.788 ± 0.238
6.476GluIle: 6.476 ± 0.972
6.476GluLys: 6.476 ± 0.842
6.214GluLeu: 6.214 ± 0.815
2.276GluMet: 2.276 ± 0.426
4.201GluAsn: 4.201 ± 0.531
1.663GluPro: 1.663 ± 0.386
4.288GluGln: 4.288 ± 0.809
3.063GluArg: 3.063 ± 0.662
3.063GluSer: 3.063 ± 0.395
3.938GluThr: 3.938 ± 0.65
3.938GluVal: 3.938 ± 0.684
0.613GluTrp: 0.613 ± 0.225
2.013GluTyr: 2.013 ± 0.439
0.0GluXaa: 0.0 ± 0.0
Phe
2.1PheAla: 2.1 ± 0.466
0.175PheCys: 0.175 ± 0.107
5.164PheAsp: 5.164 ± 0.672
4.814PheGlu: 4.814 ± 0.634
1.225PhePhe: 1.225 ± 0.378
2.626PheGly: 2.626 ± 0.491
0.438PheHis: 0.438 ± 0.19
1.838PheIle: 1.838 ± 0.415
4.464PheLys: 4.464 ± 0.63
2.713PheLeu: 2.713 ± 0.357
1.4PheMet: 1.4 ± 0.332
4.113PheAsn: 4.113 ± 0.599
0.525PhePro: 0.525 ± 0.184
2.013PheGln: 2.013 ± 0.354
1.488PheArg: 1.488 ± 0.364
3.063PheSer: 3.063 ± 0.573
2.713PheThr: 2.713 ± 0.441
1.838PheVal: 1.838 ± 0.329
0.35PheTrp: 0.35 ± 0.155
2.1PheTyr: 2.1 ± 0.511
0.0PheXaa: 0.0 ± 0.0
Gly
4.464GlyAla: 4.464 ± 1.505
0.175GlyCys: 0.175 ± 0.116
4.113GlyAsp: 4.113 ± 0.785
2.451GlyGlu: 2.451 ± 0.555
3.501GlyPhe: 3.501 ± 0.72
3.676GlyGly: 3.676 ± 0.682
1.05GlyHis: 1.05 ± 0.224
7.352GlyIle: 7.352 ± 1.342
4.901GlyLys: 4.901 ± 0.759
5.339GlyLeu: 5.339 ± 1.178
2.013GlyMet: 2.013 ± 0.705
3.238GlyAsn: 3.238 ± 0.538
0.088GlyPro: 0.088 ± 0.084
2.713GlyGln: 2.713 ± 0.344
1.75GlyArg: 1.75 ± 0.397
3.501GlySer: 3.501 ± 0.642
4.026GlyThr: 4.026 ± 0.778
4.288GlyVal: 4.288 ± 0.768
0.7GlyTrp: 0.7 ± 0.272
2.276GlyTyr: 2.276 ± 0.465
0.0GlyXaa: 0.0 ± 0.0
His
0.7HisAla: 0.7 ± 0.208
0.263HisCys: 0.263 ± 0.149
0.963HisAsp: 0.963 ± 0.358
0.875HisGlu: 0.875 ± 0.201
0.788HisPhe: 0.788 ± 0.297
0.263HisGly: 0.263 ± 0.17
0.088HisHis: 0.088 ± 0.088
0.963HisIle: 0.963 ± 0.31
0.613HisLys: 0.613 ± 0.247
0.7HisLeu: 0.7 ± 0.218
0.438HisMet: 0.438 ± 0.171
0.875HisAsn: 0.875 ± 0.345
0.7HisPro: 0.7 ± 0.24
0.438HisGln: 0.438 ± 0.177
1.05HisArg: 1.05 ± 0.329
0.875HisSer: 0.875 ± 0.287
0.7HisThr: 0.7 ± 0.254
0.875HisVal: 0.875 ± 0.252
0.175HisTrp: 0.175 ± 0.102
0.875HisTyr: 0.875 ± 0.356
0.0HisXaa: 0.0 ± 0.0
Ile
5.951IleAla: 5.951 ± 1.167
0.525IleCys: 0.525 ± 0.269
6.476IleAsp: 6.476 ± 0.787
6.214IleGlu: 6.214 ± 0.95
2.1IlePhe: 2.1 ± 0.37
5.426IleGly: 5.426 ± 1.23
1.313IleHis: 1.313 ± 0.323
5.601IleIle: 5.601 ± 0.675
7.264IleLys: 7.264 ± 0.837
4.551IleLeu: 4.551 ± 0.56
0.7IleMet: 0.7 ± 0.26
5.164IleAsn: 5.164 ± 1.022
2.1IlePro: 2.1 ± 0.415
1.663IleGln: 1.663 ± 0.354
2.713IleArg: 2.713 ± 0.52
6.214IleSer: 6.214 ± 1.055
5.339IleThr: 5.339 ± 0.826
4.113IleVal: 4.113 ± 0.52
0.875IleTrp: 0.875 ± 0.329
2.626IleTyr: 2.626 ± 0.538
0.0IleXaa: 0.0 ± 0.0
Lys
6.389LysAla: 6.389 ± 0.951
0.438LysCys: 0.438 ± 0.237
4.814LysAsp: 4.814 ± 0.808
7.002LysGlu: 7.002 ± 1.224
2.713LysPhe: 2.713 ± 0.464
4.814LysGly: 4.814 ± 0.669
0.875LysHis: 0.875 ± 0.321
5.164LysIle: 5.164 ± 0.901
7.527LysLys: 7.527 ± 1.426
7.264LysLeu: 7.264 ± 0.815
1.925LysMet: 1.925 ± 0.409
6.039LysAsn: 6.039 ± 0.585
3.063LysPro: 3.063 ± 0.606
3.238LysGln: 3.238 ± 0.576
2.976LysArg: 2.976 ± 0.649
5.689LysSer: 5.689 ± 0.873
4.726LysThr: 4.726 ± 0.672
7.002LysVal: 7.002 ± 0.836
1.05LysTrp: 1.05 ± 0.304
3.413LysTyr: 3.413 ± 0.562
0.0LysXaa: 0.0 ± 0.0
Leu
5.776LeuAla: 5.776 ± 0.867
0.438LeuCys: 0.438 ± 0.168
7.002LeuAsp: 7.002 ± 0.668
4.288LeuGlu: 4.288 ± 0.73
3.501LeuPhe: 3.501 ± 0.464
4.901LeuGly: 4.901 ± 0.904
1.4LeuHis: 1.4 ± 0.452
4.989LeuIle: 4.989 ± 0.709
6.827LeuLys: 6.827 ± 0.826
4.639LeuLeu: 4.639 ± 0.737
1.663LeuMet: 1.663 ± 0.347
6.389LeuAsn: 6.389 ± 0.886
3.413LeuPro: 3.413 ± 0.553
3.763LeuGln: 3.763 ± 0.63
2.626LeuArg: 2.626 ± 0.569
6.039LeuSer: 6.039 ± 0.579
4.814LeuThr: 4.814 ± 0.623
4.113LeuVal: 4.113 ± 0.695
0.613LeuTrp: 0.613 ± 0.332
2.713LeuTyr: 2.713 ± 0.526
0.0LeuXaa: 0.0 ± 0.0
Met
2.363MetAla: 2.363 ± 0.692
0.175MetCys: 0.175 ± 0.123
0.963MetAsp: 0.963 ± 0.341
1.575MetGlu: 1.575 ± 0.478
0.788MetPhe: 0.788 ± 0.222
1.488MetGly: 1.488 ± 0.455
0.525MetHis: 0.525 ± 0.206
1.75MetIle: 1.75 ± 0.369
1.75MetLys: 1.75 ± 0.491
1.925MetLeu: 1.925 ± 0.348
1.575MetMet: 1.575 ± 0.428
1.225MetAsn: 1.225 ± 0.268
0.788MetPro: 0.788 ± 0.244
1.75MetGln: 1.75 ± 0.299
1.225MetArg: 1.225 ± 0.268
2.1MetSer: 2.1 ± 0.48
1.925MetThr: 1.925 ± 0.381
0.788MetVal: 0.788 ± 0.226
0.263MetTrp: 0.263 ± 0.147
0.35MetTyr: 0.35 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
4.989AsnAla: 4.989 ± 0.843
0.438AsnCys: 0.438 ± 0.2
4.901AsnAsp: 4.901 ± 0.704
3.938AsnGlu: 3.938 ± 0.568
3.063AsnPhe: 3.063 ± 0.478
4.639AsnGly: 4.639 ± 0.712
1.138AsnHis: 1.138 ± 0.327
4.989AsnIle: 4.989 ± 0.688
4.989AsnLys: 4.989 ± 0.604
4.288AsnLeu: 4.288 ± 0.74
1.488AsnMet: 1.488 ± 0.314
4.464AsnAsn: 4.464 ± 1.067
2.276AsnPro: 2.276 ± 0.493
2.626AsnGln: 2.626 ± 0.947
1.313AsnArg: 1.313 ± 0.35
4.026AsnSer: 4.026 ± 0.74
3.413AsnThr: 3.413 ± 0.606
3.851AsnVal: 3.851 ± 0.539
0.875AsnTrp: 0.875 ± 0.278
2.538AsnTyr: 2.538 ± 0.56
0.0AsnXaa: 0.0 ± 0.0
Pro
2.276ProAla: 2.276 ± 0.387
0.088ProCys: 0.088 ± 0.119
2.013ProAsp: 2.013 ± 0.436
1.138ProGlu: 1.138 ± 0.345
1.838ProPhe: 1.838 ± 0.411
1.05ProGly: 1.05 ± 0.349
0.263ProHis: 0.263 ± 0.155
1.663ProIle: 1.663 ± 0.412
2.1ProLys: 2.1 ± 0.429
2.013ProLeu: 2.013 ± 0.441
0.525ProMet: 0.525 ± 0.185
1.225ProAsn: 1.225 ± 0.341
0.7ProPro: 0.7 ± 0.164
1.138ProGln: 1.138 ± 0.507
1.313ProArg: 1.313 ± 0.419
1.225ProSer: 1.225 ± 0.307
1.75ProThr: 1.75 ± 0.383
1.925ProVal: 1.925 ± 0.383
0.35ProTrp: 0.35 ± 0.17
1.313ProTyr: 1.313 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
2.713GlnAla: 2.713 ± 0.547
0.175GlnCys: 0.175 ± 0.119
2.188GlnAsp: 2.188 ± 0.391
2.363GlnGlu: 2.363 ± 0.564
1.575GlnPhe: 1.575 ± 0.277
2.626GlnGly: 2.626 ± 0.759
0.438GlnHis: 0.438 ± 0.2
4.113GlnIle: 4.113 ± 0.593
4.464GlnLys: 4.464 ± 0.537
3.063GlnLeu: 3.063 ± 0.572
1.138GlnMet: 1.138 ± 0.347
2.013GlnAsn: 2.013 ± 0.581
1.138GlnPro: 1.138 ± 0.455
2.626GlnGln: 2.626 ± 0.897
1.313GlnArg: 1.313 ± 0.311
3.763GlnSer: 3.763 ± 0.483
1.575GlnThr: 1.575 ± 0.396
2.538GlnVal: 2.538 ± 0.4
0.35GlnTrp: 0.35 ± 0.156
1.488GlnTyr: 1.488 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
2.188ArgAla: 2.188 ± 0.38
0.088ArgCys: 0.088 ± 0.086
1.925ArgAsp: 1.925 ± 0.368
2.188ArgGlu: 2.188 ± 0.459
1.4ArgPhe: 1.4 ± 0.31
1.838ArgGly: 1.838 ± 0.385
0.525ArgHis: 0.525 ± 0.241
2.363ArgIle: 2.363 ± 0.367
3.326ArgLys: 3.326 ± 0.648
3.238ArgLeu: 3.238 ± 0.58
1.05ArgMet: 1.05 ± 0.33
3.063ArgAsn: 3.063 ± 0.483
0.788ArgPro: 0.788 ± 0.298
1.225ArgGln: 1.225 ± 0.296
1.313ArgArg: 1.313 ± 0.381
1.575ArgSer: 1.575 ± 0.37
1.488ArgThr: 1.488 ± 0.359
2.276ArgVal: 2.276 ± 0.392
0.438ArgTrp: 0.438 ± 0.223
1.925ArgTyr: 1.925 ± 0.471
0.0ArgXaa: 0.0 ± 0.0
Ser
4.989SerAla: 4.989 ± 1.611
0.088SerCys: 0.088 ± 0.078
4.726SerAsp: 4.726 ± 0.791
4.026SerGlu: 4.026 ± 0.641
1.663SerPhe: 1.663 ± 0.351
4.113SerGly: 4.113 ± 0.958
0.788SerHis: 0.788 ± 0.32
4.201SerIle: 4.201 ± 0.44
6.039SerLys: 6.039 ± 0.754
6.651SerLeu: 6.651 ± 0.7
1.4SerMet: 1.4 ± 0.383
3.763SerAsn: 3.763 ± 0.557
1.663SerPro: 1.663 ± 0.344
2.451SerGln: 2.451 ± 0.36
2.363SerArg: 2.363 ± 0.326
5.164SerSer: 5.164 ± 0.981
4.113SerThr: 4.113 ± 0.771
3.938SerVal: 3.938 ± 0.565
1.138SerTrp: 1.138 ± 0.254
3.151SerTyr: 3.151 ± 0.657
0.0SerXaa: 0.0 ± 0.0
Thr
3.851ThrAla: 3.851 ± 1.181
0.35ThrCys: 0.35 ± 0.166
3.676ThrAsp: 3.676 ± 0.593
3.851ThrGlu: 3.851 ± 0.515
3.063ThrPhe: 3.063 ± 0.585
3.326ThrGly: 3.326 ± 0.735
0.613ThrHis: 0.613 ± 0.214
5.864ThrIle: 5.864 ± 0.716
5.076ThrLys: 5.076 ± 0.818
4.201ThrLeu: 4.201 ± 0.931
1.225ThrMet: 1.225 ± 0.303
3.851ThrAsn: 3.851 ± 0.765
1.488ThrPro: 1.488 ± 0.335
1.925ThrGln: 1.925 ± 0.398
1.225ThrArg: 1.225 ± 0.376
3.676ThrSer: 3.676 ± 0.908
2.538ThrThr: 2.538 ± 0.483
5.339ThrVal: 5.339 ± 0.716
0.525ThrTrp: 0.525 ± 0.22
2.188ThrTyr: 2.188 ± 0.377
0.0ThrXaa: 0.0 ± 0.0
Val
4.201ValAla: 4.201 ± 0.898
0.175ValCys: 0.175 ± 0.121
4.376ValAsp: 4.376 ± 0.525
3.676ValGlu: 3.676 ± 0.609
3.501ValPhe: 3.501 ± 0.68
4.201ValGly: 4.201 ± 0.885
0.963ValHis: 0.963 ± 0.358
5.689ValIle: 5.689 ± 0.742
5.076ValLys: 5.076 ± 0.678
4.288ValLeu: 4.288 ± 0.717
1.225ValMet: 1.225 ± 0.321
4.026ValAsn: 4.026 ± 0.518
1.925ValPro: 1.925 ± 0.385
1.925ValGln: 1.925 ± 0.622
1.575ValArg: 1.575 ± 0.281
4.113ValSer: 4.113 ± 0.496
3.938ValThr: 3.938 ± 0.444
2.888ValVal: 2.888 ± 0.443
1.05ValTrp: 1.05 ± 0.355
2.713ValTyr: 2.713 ± 0.703
0.0ValXaa: 0.0 ± 0.0
Trp
0.438TrpAla: 0.438 ± 0.174
0.0TrpCys: 0.0 ± 0.0
0.613TrpAsp: 0.613 ± 0.246
0.613TrpGlu: 0.613 ± 0.234
0.613TrpPhe: 0.613 ± 0.211
0.875TrpGly: 0.875 ± 0.431
0.263TrpHis: 0.263 ± 0.135
0.875TrpIle: 0.875 ± 0.265
1.05TrpLys: 1.05 ± 0.339
1.4TrpLeu: 1.4 ± 0.364
0.263TrpMet: 0.263 ± 0.161
0.263TrpAsn: 0.263 ± 0.146
0.088TrpPro: 0.088 ± 0.09
0.438TrpGln: 0.438 ± 0.215
0.438TrpArg: 0.438 ± 0.174
1.138TrpSer: 1.138 ± 0.298
0.438TrpThr: 0.438 ± 0.181
0.788TrpVal: 0.788 ± 0.208
0.35TrpTrp: 0.35 ± 0.161
0.438TrpTyr: 0.438 ± 0.22
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.75TyrAla: 1.75 ± 0.466
0.263TyrCys: 0.263 ± 0.196
2.363TyrAsp: 2.363 ± 0.577
2.888TyrGlu: 2.888 ± 0.509
3.151TyrPhe: 3.151 ± 0.695
2.363TyrGly: 2.363 ± 0.501
0.263TyrHis: 0.263 ± 0.12
2.451TyrIle: 2.451 ± 0.413
3.063TyrLys: 3.063 ± 0.407
3.588TyrLeu: 3.588 ± 0.776
0.438TyrMet: 0.438 ± 0.229
2.363TyrAsn: 2.363 ± 0.52
0.788TyrPro: 0.788 ± 0.285
2.188TyrGln: 2.188 ± 0.608
1.575TyrArg: 1.575 ± 0.406
3.151TyrSer: 3.151 ± 0.665
2.538TyrThr: 2.538 ± 0.462
1.838TyrVal: 1.838 ± 0.49
0.438TyrTrp: 0.438 ± 0.172
2.1TyrTyr: 2.1 ± 0.393
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11427 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski