Leptonema illini DSM 21528

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptonema; Leptonema illini

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2CF11|H2CF11_9LEPT Transposase IS4 family protein OS=Leptonema illini DSM 21528 OX=929563 GN=Lepil_0913 PE=4 SV=1
MM1 pKa = 7.47LLFVSVLSACPAGGGGDD18 pKa = 3.79AFLPPPGLTSGGTTTCPADD37 pKa = 3.23AWPFDD42 pKa = 3.58TGAYY46 pKa = 10.02LADD49 pKa = 4.39FVTEE53 pKa = 4.38APGEE57 pKa = 4.27TGGTFGNSLCAVNGVYY73 pKa = 10.85GNGLNRR79 pKa = 11.84GSGDD83 pKa = 3.6VYY85 pKa = 10.82SLKK88 pKa = 10.65SSPASTLCEE97 pKa = 4.0ANEE100 pKa = 3.96KK101 pKa = 10.79CIVLEE106 pKa = 3.85WAGRR110 pKa = 11.84RR111 pKa = 11.84VLSGAGADD119 pKa = 3.24FVVFEE124 pKa = 4.51NPFNRR129 pKa = 11.84TSGGRR134 pKa = 11.84FVEE137 pKa = 4.2AVIVEE142 pKa = 4.13VSQDD146 pKa = 3.22GTVWCGWNPAYY157 pKa = 9.74TGEE160 pKa = 4.28TDD162 pKa = 2.95SGAAQFSLDD171 pKa = 3.31IYY173 pKa = 11.36DD174 pKa = 3.44VAKK177 pKa = 10.62YY178 pKa = 10.87DD179 pKa = 3.93DD180 pKa = 3.94VAGLEE185 pKa = 4.07PVLFNQSTWTGTATDD200 pKa = 3.64VFDD203 pKa = 3.58VAKK206 pKa = 10.82AGGDD210 pKa = 3.59HH211 pKa = 7.32FDD213 pKa = 4.25LADD216 pKa = 3.87LVASGSCDD224 pKa = 3.12AGTVSAIQASGFVYY238 pKa = 10.71LRR240 pKa = 11.84MTTGNSRR247 pKa = 11.84SPASFPLPGDD257 pKa = 3.88SFDD260 pKa = 3.52QTGDD264 pKa = 2.75IDD266 pKa = 4.41GVVARR271 pKa = 11.84NVDD274 pKa = 3.41DD275 pKa = 3.86RR276 pKa = 5.34

Molecular weight: 28.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2CGQ2|H2CGQ2_9LEPT Activator of Hsp90 ATPase 1 family protein OS=Leptonema illini DSM 21528 OX=929563 GN=Lepil_0015 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 9.61RR3 pKa = 11.84TFQPGNISRR12 pKa = 11.84LRR14 pKa = 11.84THH16 pKa = 6.75GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTSGGRR28 pKa = 11.84KK29 pKa = 8.58VLANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.12KK37 pKa = 10.61GRR39 pKa = 11.84MRR41 pKa = 11.84LTVSSSTHH49 pKa = 5.58RR50 pKa = 11.84KK51 pKa = 8.72

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4129

0

4129

1346542

30

3820

326.1

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.686 ± 0.043

0.858 ± 0.013

5.546 ± 0.029

6.825 ± 0.037

4.65 ± 0.029

7.204 ± 0.034

2.128 ± 0.018

6.088 ± 0.031

4.495 ± 0.036

10.683 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.016

3.099 ± 0.02

4.499 ± 0.024

3.389 ± 0.023

7.222 ± 0.041

6.568 ± 0.035

4.941 ± 0.031

6.404 ± 0.033

1.112 ± 0.015

3.133 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski