Acholeplasma brassicae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4KQ71|U4KQ71_9MOLU Pyrroline-5-carboxylate reductase OS=Acholeplasma brassicae OX=61635 GN=proC PE=3 SV=1
MM1 pKa = 7.6NEE3 pKa = 3.95EE4 pKa = 4.96AILTYY9 pKa = 10.18TVGDD13 pKa = 3.93PFSDD17 pKa = 3.89IIASTWCEE25 pKa = 3.9GIDD28 pKa = 4.05NVDD31 pKa = 3.38EE32 pKa = 4.35TVDD35 pKa = 4.72CEE37 pKa = 4.24FLSHH41 pKa = 6.93GIVNTSIAGTYY52 pKa = 9.38ILIYY56 pKa = 10.22QATDD60 pKa = 2.98NAGNTAEE67 pKa = 4.52LRR69 pKa = 11.84LTVTVSDD76 pKa = 4.12VVEE79 pKa = 4.48SNPDD83 pKa = 3.04VLAYY87 pKa = 10.25YY88 pKa = 10.58SSAEE92 pKa = 3.99GLSGNTLFLEE102 pKa = 4.43LRR104 pKa = 11.84SIIQADD110 pKa = 4.23MIKK113 pKa = 10.53VSYY116 pKa = 10.97SDD118 pKa = 3.0ARR120 pKa = 11.84YY121 pKa = 9.83ILDD124 pKa = 5.02EE125 pKa = 5.24ADD127 pKa = 3.23QDD129 pKa = 4.27PNNSNNVLTIYY140 pKa = 10.45DD141 pKa = 3.83RR142 pKa = 11.84QSVLGAWDD150 pKa = 3.28GTTYY154 pKa = 10.14TRR156 pKa = 11.84EE157 pKa = 4.24HH158 pKa = 5.59VWPNSRR164 pKa = 11.84LGVSRR169 pKa = 11.84VSNSTKK175 pKa = 10.77NIGTDD180 pKa = 3.18LHH182 pKa = 6.28NLRR185 pKa = 11.84ATIQSTNSSRR195 pKa = 11.84SNKK198 pKa = 9.67YY199 pKa = 10.44FDD201 pKa = 3.51FTTTNDD207 pKa = 2.91AYY209 pKa = 11.53YY210 pKa = 10.08PGEE213 pKa = 4.29DD214 pKa = 4.17DD215 pKa = 4.08KK216 pKa = 12.16GDD218 pKa = 3.36VARR221 pKa = 11.84ILFYY225 pKa = 10.54MVVMYY230 pKa = 10.04PNLDD234 pKa = 3.16IVNVITSAMDD244 pKa = 3.11EE245 pKa = 4.14ATYY248 pKa = 10.89KK249 pKa = 10.91EE250 pKa = 4.82DD251 pKa = 3.3GTYY254 pKa = 7.79MAKK257 pKa = 9.67MSVLLQWHH265 pKa = 6.66IEE267 pKa = 4.09DD268 pKa = 4.43PVDD271 pKa = 3.9DD272 pKa = 4.36FEE274 pKa = 5.95RR275 pKa = 11.84NRR277 pKa = 11.84NEE279 pKa = 4.49VIYY282 pKa = 10.49NYY284 pKa = 10.31QNNRR288 pKa = 11.84NPFIDD293 pKa = 3.72NPDD296 pKa = 3.4YY297 pKa = 11.32VALLWGNNPSTVSNSSQFINN317 pKa = 3.72

Molecular weight: 35.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4KQR7|U4KQR7_9MOLU Bifunctional protein GlmU OS=Acholeplasma brassicae OX=61635 GN=glmU PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.28QPSKK9 pKa = 10.61LKK11 pKa = 10.22RR12 pKa = 11.84QKK14 pKa = 8.58THH16 pKa = 5.13GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.4GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1671

0

1671

559421

37

6236

334.8

38.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.528 ± 0.062

0.53 ± 0.016

5.749 ± 0.049

6.714 ± 0.054

4.987 ± 0.052

5.886 ± 0.069

1.853 ± 0.029

8.547 ± 0.067

7.744 ± 0.074

10.272 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.037

5.389 ± 0.049

2.845 ± 0.027

3.455 ± 0.031

3.588 ± 0.038

6.37 ± 0.057

5.664 ± 0.056

7.038 ± 0.056

0.657 ± 0.019

4.658 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski