Amino acid dipepetide frequency for Microbacterium chocolatum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.533AlaAla: 21.533 ± 0.28	0.663AlaCys: 0.663 ± 0.028	9.076AlaAsp: 9.076 ± 0.114	8.678AlaGlu: 8.678 ± 0.109	4.02AlaPhe: 4.02 ± 0.069	12.316AlaGly: 12.316 ± 0.134	2.621AlaHis: 2.621 ± 0.065	5.693AlaIle: 5.693 ± 0.083	2.326AlaLys: 2.326 ± 0.062	14.706AlaLeu: 14.706 ± 0.177	2.673AlaMet: 2.673 ± 0.056	1.932AlaAsn: 1.932 ± 0.052	7.137AlaPro: 7.137 ± 0.117	3.718AlaGln: 3.718 ± 0.068	10.796AlaArg: 10.796 ± 0.138	7.151AlaSer: 7.151 ± 0.098	7.4AlaThr: 7.4 ± 0.09	12.207AlaVal: 12.207 ± 0.15	2.046AlaTrp: 2.046 ± 0.047	2.431AlaTyr: 2.431 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.692CysAla: 0.692 ± 0.03	0.037CysCys: 0.037 ± 0.007	0.303CysAsp: 0.303 ± 0.017	0.234CysGlu: 0.234 ± 0.017	0.175CysPhe: 0.175 ± 0.014	0.524CysGly: 0.524 ± 0.027	0.119CysHis: 0.119 ± 0.012	0.155CysIle: 0.155 ± 0.014	0.047CysLys: 0.047 ± 0.008	0.423CysLeu: 0.423 ± 0.022	0.064CysMet: 0.064 ± 0.008	0.088CysAsn: 0.088 ± 0.01	0.268CysPro: 0.268 ± 0.016	0.134CysGln: 0.134 ± 0.014	0.403CysArg: 0.403 ± 0.02	0.254CysSer: 0.254 ± 0.017	0.328CysThr: 0.328 ± 0.019	0.431CysVal: 0.431 ± 0.023	0.058CysTrp: 0.058 ± 0.008	0.099CysTyr: 0.099 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	10.765AspAla: 10.765 ± 0.123	0.218AspCys: 0.218 ± 0.016	4.838AspAsp: 4.838 ± 0.104	4.347AspGlu: 4.347 ± 0.087	1.763AspPhe: 1.763 ± 0.047	6.237AspGly: 6.237 ± 0.102	1.232AspHis: 1.232 ± 0.042	2.608AspIle: 2.608 ± 0.054	0.911AspLys: 0.911 ± 0.038	6.776AspLeu: 6.776 ± 0.09	0.844AspMet: 0.844 ± 0.027	0.904AspAsn: 0.904 ± 0.037	4.566AspPro: 4.566 ± 0.079	1.404AspGln: 1.404 ± 0.044	4.97AspArg: 4.97 ± 0.087	2.406AspSer: 2.406 ± 0.048	3.137AspThr: 3.137 ± 0.065	5.864AspVal: 5.864 ± 0.08	0.877AspTrp: 0.877 ± 0.032	1.219AspTyr: 1.219 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	7.289GluAla: 7.289 ± 0.097	0.25GluCys: 0.25 ± 0.017	3.167GluAsp: 3.167 ± 0.067	3.276GluGlu: 3.276 ± 0.072	1.679GluPhe: 1.679 ± 0.046	4.436GluGly: 4.436 ± 0.084	1.268GluHis: 1.268 ± 0.042	3.023GluIle: 3.023 ± 0.057	1.281GluLys: 1.281 ± 0.044	6.178GluLeu: 6.178 ± 0.096	0.921GluMet: 0.921 ± 0.037	1.193GluAsn: 1.193 ± 0.039	3.004GluPro: 3.004 ± 0.067	1.991GluGln: 1.991 ± 0.048	5.504GluArg: 5.504 ± 0.094	2.697GluSer: 2.697 ± 0.058	3.152GluThr: 3.152 ± 0.068	4.953GluVal: 4.953 ± 0.072	0.973GluTrp: 0.973 ± 0.038	1.198GluTyr: 1.198 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.426PheAla: 4.426 ± 0.064	0.161PheCys: 0.161 ± 0.012	2.473PheAsp: 2.473 ± 0.06	1.596PheGlu: 1.596 ± 0.045	1.102PhePhe: 1.102 ± 0.039	3.337PheGly: 3.337 ± 0.069	0.572PheHis: 0.572 ± 0.027	1.124PheIle: 1.124 ± 0.043	0.38PheLys: 0.38 ± 0.023	2.994PheLeu: 2.994 ± 0.067	0.379PheMet: 0.379 ± 0.024	0.58PheAsn: 0.58 ± 0.028	1.423PhePro: 1.423 ± 0.04	0.729PheGln: 0.729 ± 0.032	1.859PheArg: 1.859 ± 0.048	1.719PheSer: 1.719 ± 0.045	2.098PheThr: 2.098 ± 0.053	2.834PheVal: 2.834 ± 0.06	0.473PheTrp: 0.473 ± 0.024	0.631PheTyr: 0.631 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	11.1GlyAla: 11.1 ± 0.132	0.535GlyCys: 0.535 ± 0.027	5.532GlyAsp: 5.532 ± 0.082	5.134GlyGlu: 5.134 ± 0.081	3.209GlyPhe: 3.209 ± 0.061	7.64GlyGly: 7.64 ± 0.123	1.807GlyHis: 1.807 ± 0.045	5.02GlyIle: 5.02 ± 0.082	1.92GlyLys: 1.92 ± 0.051	8.436GlyLeu: 8.436 ± 0.115	1.944GlyMet: 1.944 ± 0.048	1.565GlyAsn: 1.565 ± 0.048	3.817GlyPro: 3.817 ± 0.071	2.27GlyGln: 2.27 ± 0.051	6.652GlyArg: 6.652 ± 0.094	4.896GlySer: 4.896 ± 0.081	5.357GlyThr: 5.357 ± 0.083	8.187GlyVal: 8.187 ± 0.101	1.599GlyTrp: 1.599 ± 0.046	2.092GlyTyr: 2.092 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.584HisAla: 2.584 ± 0.063	0.113HisCys: 0.113 ± 0.012	1.335HisAsp: 1.335 ± 0.046	1.101HisGlu: 1.101 ± 0.043	0.53HisPhe: 0.53 ± 0.027	1.862HisGly: 1.862 ± 0.046	0.563HisHis: 0.563 ± 0.024	0.742HisIle: 0.742 ± 0.032	0.252HisLys: 0.252 ± 0.017	1.996HisLeu: 1.996 ± 0.043	0.302HisMet: 0.302 ± 0.02	0.307HisAsn: 0.307 ± 0.02	1.595HisPro: 1.595 ± 0.042	0.446HisGln: 0.446 ± 0.023	1.637HisArg: 1.637 ± 0.043	0.868HisSer: 0.868 ± 0.033	0.996HisThr: 0.996 ± 0.034	1.787HisVal: 1.787 ± 0.045	0.289HisTrp: 0.289 ± 0.02	0.417HisTyr: 0.417 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.03IleAla: 7.03 ± 0.097	0.215IleCys: 0.215 ± 0.018	3.632IleAsp: 3.632 ± 0.069	2.773IleGlu: 2.773 ± 0.066	1.153IlePhe: 1.153 ± 0.043	4.513IleGly: 4.513 ± 0.088	0.705IleHis: 0.705 ± 0.026	1.731IleIle: 1.731 ± 0.053	0.686IleLys: 0.686 ± 0.038	4.157IleLeu: 4.157 ± 0.074	0.544IleMet: 0.544 ± 0.025	0.785IleAsn: 0.785 ± 0.033	2.52IlePro: 2.52 ± 0.055	0.965IleGln: 0.965 ± 0.034	3.004IleArg: 3.004 ± 0.051	2.226IleSer: 2.226 ± 0.056	2.668IleThr: 2.668 ± 0.057	4.558IleVal: 4.558 ± 0.071	0.497IleTrp: 0.497 ± 0.026	0.708IleTyr: 0.708 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	2.211LysAla: 2.211 ± 0.062	0.061LysCys: 0.061 ± 0.009	1.04LysAsp: 1.04 ± 0.041	0.844LysGlu: 0.844 ± 0.036	0.393LysPhe: 0.393 ± 0.022	1.322LysGly: 1.322 ± 0.05	0.366LysHis: 0.366 ± 0.02	0.836LysIle: 0.836 ± 0.034	0.674LysLys: 0.674 ± 0.035	1.604LysLeu: 1.604 ± 0.049	0.321LysMet: 0.321 ± 0.02	0.457LysAsn: 0.457 ± 0.027	0.962LysPro: 0.962 ± 0.039	0.592LysGln: 0.592 ± 0.032	1.374LysArg: 1.374 ± 0.043	0.953LysSer: 0.953 ± 0.041	1.219LysThr: 1.219 ± 0.037	1.479LysVal: 1.479 ± 0.046	0.22LysTrp: 0.22 ± 0.016	0.374LysTyr: 0.374 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	14.774LeuAla: 14.774 ± 0.141	0.495LeuCys: 0.495 ± 0.023	6.738LeuAsp: 6.738 ± 0.095	5.233LeuGlu: 5.233 ± 0.079	2.828LeuPhe: 2.828 ± 0.076	9.076LeuGly: 9.076 ± 0.109	1.863LeuHis: 1.863 ± 0.056	4.258LeuIle: 4.258 ± 0.093	1.506LeuLys: 1.506 ± 0.046	9.891LeuLeu: 9.891 ± 0.15	1.589LeuMet: 1.589 ± 0.046	1.648LeuAsn: 1.648 ± 0.051	5.522LeuPro: 5.522 ± 0.078	2.473LeuGln: 2.473 ± 0.061	7.95LeuArg: 7.95 ± 0.107	5.639LeuSer: 5.639 ± 0.083	6.282LeuThr: 6.282 ± 0.082	9.232LeuVal: 9.232 ± 0.138	1.3LeuTrp: 1.3 ± 0.041	1.623LeuTyr: 1.623 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	1.978MetAla: 1.978 ± 0.058	0.094MetCys: 0.094 ± 0.011	0.851MetAsp: 0.851 ± 0.031	0.632MetGlu: 0.632 ± 0.029	0.497MetPhe: 0.497 ± 0.025	1.214MetGly: 1.214 ± 0.039	0.306MetHis: 0.306 ± 0.019	0.881MetIle: 0.881 ± 0.032	0.387MetLys: 0.387 ± 0.024	1.773MetLeu: 1.773 ± 0.048	0.305MetMet: 0.305 ± 0.02	0.487MetAsn: 0.487 ± 0.022	1.254MetPro: 1.254 ± 0.032	0.524MetGln: 0.524 ± 0.023	1.397MetArg: 1.397 ± 0.042	1.457MetSer: 1.457 ± 0.039	1.625MetThr: 1.625 ± 0.046	1.29MetVal: 1.29 ± 0.039	0.205MetTrp: 0.205 ± 0.018	0.264MetTyr: 0.264 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.301AsnAla: 2.301 ± 0.058	0.097AsnCys: 0.097 ± 0.01	1.076AsnAsp: 1.076 ± 0.037	0.889AsnGlu: 0.889 ± 0.03	0.518AsnPhe: 0.518 ± 0.026	1.662AsnGly: 1.662 ± 0.047	0.329AsnHis: 0.329 ± 0.018	0.797AsnIle: 0.797 ± 0.032	0.332AsnLys: 0.332 ± 0.02	1.807AsnLeu: 1.807 ± 0.048	0.289AsnMet: 0.289 ± 0.021	0.386AsnAsn: 0.386 ± 0.026	1.483AsnPro: 1.483 ± 0.043	0.507AsnGln: 0.507 ± 0.029	1.203AsnArg: 1.203 ± 0.042	0.798AsnSer: 0.798 ± 0.03	1.001AsnThr: 1.001 ± 0.037	1.475AsnVal: 1.475 ± 0.043	0.256AsnTrp: 0.256 ± 0.019	0.374AsnTyr: 0.374 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.781ProAla: 7.781 ± 0.124	0.191ProCys: 0.191 ± 0.015	4.295ProAsp: 4.295 ± 0.086	3.967ProGlu: 3.967 ± 0.075	1.827ProPhe: 1.827 ± 0.044	5.267ProGly: 5.267 ± 0.087	1.16ProHis: 1.16 ± 0.036	2.106ProIle: 2.106 ± 0.052	0.892ProLys: 0.892 ± 0.037	4.925ProLeu: 4.925 ± 0.069	0.906ProMet: 0.906 ± 0.031	0.915ProAsn: 0.915 ± 0.031	2.429ProPro: 2.429 ± 0.065	1.421ProGln: 1.421 ± 0.037	3.852ProArg: 3.852 ± 0.073	2.938ProSer: 2.938 ± 0.06	3.417ProThr: 3.417 ± 0.07	5.047ProVal: 5.047 ± 0.077	0.887ProTrp: 0.887 ± 0.035	0.987ProTyr: 0.987 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.16GlnAla: 3.16 ± 0.07	0.097GlnCys: 0.097 ± 0.012	1.225GlnAsp: 1.225 ± 0.036	1.294GlnGlu: 1.294 ± 0.037	0.793GlnPhe: 0.793 ± 0.034	1.94GlnGly: 1.94 ± 0.045	0.567GlnHis: 0.567 ± 0.027	1.43GlnIle: 1.43 ± 0.043	0.621GlnLys: 0.621 ± 0.028	2.813GlnLeu: 2.813 ± 0.062	0.497GlnMet: 0.497 ± 0.03	0.607GlnAsn: 0.607 ± 0.028	1.415GlnPro: 1.415 ± 0.035	1.023GlnGln: 1.023 ± 0.043	2.341GlnArg: 2.341 ± 0.059	1.307GlnSer: 1.307 ± 0.042	1.547GlnThr: 1.547 ± 0.042	2.164GlnVal: 2.164 ± 0.049	0.441GlnTrp: 0.441 ± 0.022	0.557GlnTyr: 0.557 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	10.229ArgAla: 10.229 ± 0.127	0.372ArgCys: 0.372 ± 0.023	4.842ArgAsp: 4.842 ± 0.083	4.919ArgGlu: 4.919 ± 0.09	2.56ArgPhe: 2.56 ± 0.053	5.996ArgGly: 5.996 ± 0.099	1.815ArgHis: 1.815 ± 0.043	3.805ArgIle: 3.805 ± 0.066	1.254ArgLys: 1.254 ± 0.046	7.528ArgLeu: 7.528 ± 0.116	1.815ArgMet: 1.815 ± 0.049	1.307ArgAsn: 1.307 ± 0.04	3.949ArgPro: 3.949 ± 0.081	1.928ArgGln: 1.928 ± 0.052	7.546ArgArg: 7.546 ± 0.119	4.118ArgSer: 4.118 ± 0.075	4.485ArgThr: 4.485 ± 0.071	6.922ArgVal: 6.922 ± 0.098	1.271ArgTrp: 1.271 ± 0.043	1.586ArgTyr: 1.586 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	7.055SerAla: 7.055 ± 0.094	0.242SerCys: 0.242 ± 0.018	3.24SerAsp: 3.24 ± 0.069	2.662SerGlu: 2.662 ± 0.057	1.803SerPhe: 1.803 ± 0.043	5.499SerGly: 5.499 ± 0.078	0.905SerHis: 0.905 ± 0.03	2.279SerIle: 2.279 ± 0.05	0.81SerLys: 0.81 ± 0.032	5.003SerLeu: 5.003 ± 0.086	1.088SerMet: 1.088 ± 0.036	0.917SerAsn: 0.917 ± 0.036	3.079SerPro: 3.079 ± 0.057	1.213SerGln: 1.213 ± 0.038	3.875SerArg: 3.875 ± 0.062	3.032SerSer: 3.032 ± 0.064	3.248SerThr: 3.248 ± 0.059	4.578SerVal: 4.578 ± 0.072	0.913SerTrp: 0.913 ± 0.036	1.089SerTyr: 1.089 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	7.875ThrAla: 7.875 ± 0.108	0.282ThrCys: 0.282 ± 0.02	3.85ThrAsp: 3.85 ± 0.075	3.011ThrGlu: 3.011 ± 0.061	1.776ThrPhe: 1.776 ± 0.044	5.702ThrGly: 5.702 ± 0.092	1.105ThrHis: 1.105 ± 0.038	2.688ThrIle: 2.688 ± 0.061	1.025ThrLys: 1.025 ± 0.038	6.07ThrLeu: 6.07 ± 0.091	0.903ThrMet: 0.903 ± 0.035	1.0ThrAsn: 1.0 ± 0.037	4.146ThrPro: 4.146 ± 0.075	1.358ThrGln: 1.358 ± 0.038	4.061ThrArg: 4.061 ± 0.075	3.13ThrSer: 3.13 ± 0.061	3.721ThrThr: 3.721 ± 0.075	5.727ThrVal: 5.727 ± 0.08	0.849ThrTrp: 0.849 ± 0.03	1.129ThrTyr: 1.129 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	12.174ValAla: 12.174 ± 0.127	0.499ValCys: 0.499 ± 0.027	6.202ValAsp: 6.202 ± 0.078	5.016ValGlu: 5.016 ± 0.08	3.01ValPhe: 3.01 ± 0.062	7.212ValGly: 7.212 ± 0.109	1.769ValHis: 1.769 ± 0.047	4.485ValIle: 4.485 ± 0.075	1.437ValLys: 1.437 ± 0.049	9.181ValLeu: 9.181 ± 0.121	1.406ValMet: 1.406 ± 0.046	1.725ValAsn: 1.725 ± 0.05	4.862ValPro: 4.862 ± 0.082	2.123ValGln: 2.123 ± 0.051	6.754ValArg: 6.754 ± 0.101	5.105ValSer: 5.105 ± 0.089	5.721ValThr: 5.721 ± 0.078	9.299ValVal: 9.299 ± 0.121	1.251ValTrp: 1.251 ± 0.043	1.555ValTyr: 1.555 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.682TrpAla: 1.682 ± 0.046	0.099TrpCys: 0.099 ± 0.012	0.845TrpAsp: 0.845 ± 0.031	0.758TrpGlu: 0.758 ± 0.03	0.563TrpPhe: 0.563 ± 0.027	1.152TrpGly: 1.152 ± 0.037	0.355TrpHis: 0.355 ± 0.018	0.712TrpIle: 0.712 ± 0.032	0.258TrpLys: 0.258 ± 0.017	1.59TrpLeu: 1.59 ± 0.044	0.379TrpMet: 0.379 ± 0.022	0.373TrpAsn: 0.373 ± 0.023	0.767TrpPro: 0.767 ± 0.037	0.503TrpGln: 0.503 ± 0.023	1.323TrpArg: 1.323 ± 0.033	0.899TrpSer: 0.899 ± 0.038	0.918TrpThr: 0.918 ± 0.032	1.199TrpVal: 1.199 ± 0.04	0.392TrpTrp: 0.392 ± 0.022	0.295TrpTyr: 0.295 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.484TyrAla: 2.484 ± 0.056	0.114TyrCys: 0.114 ± 0.011	1.325TyrAsp: 1.325 ± 0.043	1.067TyrGlu: 1.067 ± 0.042	0.692TyrPhe: 0.692 ± 0.031	1.807TyrGly: 1.807 ± 0.044	0.295TyrHis: 0.295 ± 0.022	0.698TyrIle: 0.698 ± 0.033	0.304TyrLys: 0.304 ± 0.019	2.069TyrLeu: 2.069 ± 0.052	0.251TyrMet: 0.251 ± 0.018	0.402TyrAsn: 0.402 ± 0.024	1.013TyrPro: 1.013 ± 0.036	0.519TyrGln: 0.519 ± 0.028	1.743TyrArg: 1.743 ± 0.046	0.939TyrSer: 0.939 ± 0.032	1.087TyrThr: 1.087 ± 0.042	1.553TyrVal: 1.553 ± 0.041	0.264TyrTrp: 0.264 ± 0.02	0.403TyrTyr: 0.403 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2721 proteins (868408 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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