Esparto virus

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Nudiviridae; Alphanudivirus; Drosophila melanogaster nudivirus C

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7G301|A0A2I7G301_9VIRU Thymidylate synthase OS=Esparto virus OX=2072209 PE=3 SV=1
MM1 pKa = 7.35SFCLSSDD8 pKa = 3.74SLSSMDD14 pKa = 6.28DD15 pKa = 3.19IDD17 pKa = 3.82EE18 pKa = 4.42STPVVAGTTHH28 pKa = 7.01MIDD31 pKa = 3.02KK32 pKa = 10.3CVDD35 pKa = 2.77IAYY38 pKa = 10.35DD39 pKa = 4.0EE40 pKa = 6.0DD41 pKa = 6.56DD42 pKa = 6.33DD43 pKa = 7.24DD44 pKa = 5.5DD45 pKa = 6.5LPDD48 pKa = 3.66KK49 pKa = 11.32VIVNQEE55 pKa = 3.87NGNDD59 pKa = 3.51SDD61 pKa = 5.13GNNNDD66 pKa = 4.77DD67 pKa = 5.22DD68 pKa = 5.15DD69 pKa = 4.87AAADD73 pKa = 5.04DD74 pKa = 6.08DD75 pKa = 6.34DD76 pKa = 7.69DD77 pKa = 6.88DD78 pKa = 6.2DD79 pKa = 5.6EE80 pKa = 6.73NIANVNIKK88 pKa = 9.43TDD90 pKa = 3.36TSSDD94 pKa = 3.23EE95 pKa = 4.44GKK97 pKa = 8.54NTEE100 pKa = 4.04TFIIHH105 pKa = 6.88SDD107 pKa = 4.28DD108 pKa = 3.64EE109 pKa = 4.86DD110 pKa = 3.8SSYY113 pKa = 11.93NEE115 pKa = 3.82FDD117 pKa = 3.08QNYY120 pKa = 9.45INEE123 pKa = 4.38IKK125 pKa = 10.81HH126 pKa = 4.86NTEE129 pKa = 4.83DD130 pKa = 5.07LDD132 pKa = 3.93NTDD135 pKa = 5.83DD136 pKa = 5.76SDD138 pKa = 4.62DD139 pKa = 4.23DD140 pKa = 4.73SNSFEE145 pKa = 5.31HH146 pKa = 6.22YY147 pKa = 10.97NSMKK151 pKa = 10.57SFEE154 pKa = 4.28TNAIVGNTTFQRR166 pKa = 11.84TAIVKK171 pKa = 9.92SYY173 pKa = 10.76QNPIQTTEE181 pKa = 3.92ALSIFYY187 pKa = 9.91IDD189 pKa = 4.78SDD191 pKa = 4.65SDD193 pKa = 3.64DD194 pKa = 3.64TDD196 pKa = 3.69VDD198 pKa = 4.01DD199 pKa = 5.05EE200 pKa = 4.68NNEE203 pKa = 4.12HH204 pKa = 6.34EE205 pKa = 5.29HH206 pKa = 5.79YY207 pKa = 9.85TDD209 pKa = 3.08NDD211 pKa = 2.78NSYY214 pKa = 10.92RR215 pKa = 11.84YY216 pKa = 10.5DD217 pKa = 4.63DD218 pKa = 4.7DD219 pKa = 4.55TNSDD223 pKa = 3.32TDD225 pKa = 4.12DD226 pKa = 3.37EE227 pKa = 4.61HH228 pKa = 8.4VHH230 pKa = 5.87NKK232 pKa = 9.01EE233 pKa = 3.83NNEE236 pKa = 3.76IQNNNTQTIHH246 pKa = 6.06YY247 pKa = 10.25ARR249 pKa = 11.84IEE251 pKa = 4.28TNNLDD256 pKa = 3.93NFDD259 pKa = 3.92TTTTPLATVTPSTTMNNSRR278 pKa = 11.84IDD280 pKa = 3.36HH281 pKa = 7.01TYY283 pKa = 10.3TKK285 pKa = 10.39FDD287 pKa = 4.43DD288 pKa = 4.04VSSTNTNRR296 pKa = 11.84NKK298 pKa = 10.8

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7G314|A0A2I7G314_9VIRU Putative gp005-like protein OS=Esparto virus OX=2072209 PE=4 SV=1
MM1 pKa = 7.37SSVRR5 pKa = 11.84GPQSGLASPLNRR17 pKa = 11.84FTEE20 pKa = 4.28CANISKK26 pKa = 10.06SIKK29 pKa = 8.06VSAMIVLILIVLALILLVSAVLVNYY54 pKa = 9.9LYY56 pKa = 10.69KK57 pKa = 10.48INSSDD62 pKa = 3.48TASQADD68 pKa = 3.45KK69 pKa = 10.76EE70 pKa = 4.35KK71 pKa = 10.57RR72 pKa = 11.84KK73 pKa = 9.93KK74 pKa = 10.78AIVGGLTITSAVVAFLATFAAFWQMGVASKK104 pKa = 10.68AVKK107 pKa = 10.38ACIAA111 pKa = 3.82

Molecular weight: 11.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

40862

106

2294

469.7

53.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.077 ± 0.182

1.618 ± 0.113

6.784 ± 0.328

4.921 ± 0.366

3.583 ± 0.164

2.785 ± 0.151

2.296 ± 0.134

9.15 ± 0.162

6.698 ± 0.316

7.853 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.126

9.858 ± 0.363

4.114 ± 0.212

4.07 ± 0.258

3.835 ± 0.2

8.448 ± 0.342

8.034 ± 0.377

4.344 ± 0.142

0.47 ± 0.041

4.51 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski