Polymorphobacter arshaanensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Polymorphobacter

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9EQG0|A0A4Y9EQG0_9SPHN Rhodanese-like domain-containing protein OS=Polymorphobacter arshaanensis OX=2511025 GN=EUV02_00200 PE=4 SV=1
MM1 pKa = 6.46TTQTIQVSTAAGLMSALASATGGEE25 pKa = 4.66TIQLAAGNYY34 pKa = 8.67GGVYY38 pKa = 10.05LYY40 pKa = 10.52QYY42 pKa = 11.54NFAQKK47 pKa = 10.14VNIVGGTFSSIQVVSSNGLNFEE69 pKa = 4.1GTTVNFVPDD78 pKa = 3.54ATSTSNSQGVRR89 pKa = 11.84VYY91 pKa = 10.86LSQNINFNNSTVVGGLSVNGVDD113 pKa = 4.36PSAASGDD120 pKa = 3.46ATGNVLGYY128 pKa = 9.61PVGKK132 pKa = 10.05GVNFEE137 pKa = 4.21NSSFCSITNSDD148 pKa = 3.15VSVFAKK154 pKa = 10.66GITITGGHH162 pKa = 7.47DD163 pKa = 3.28YY164 pKa = 11.21LIDD167 pKa = 3.72NNTIHH172 pKa = 7.83DD173 pKa = 4.46LRR175 pKa = 11.84TTPISGSVTSNLTISNNHH193 pKa = 5.54TWDD196 pKa = 3.2SHH198 pKa = 4.52PWQFGTQDD206 pKa = 3.01HH207 pKa = 7.21GDD209 pKa = 4.2RR210 pKa = 11.84IHH212 pKa = 6.25VWTNGTPISGLVIANNYY229 pKa = 9.09LDD231 pKa = 4.17QGAGDD236 pKa = 4.17PMLGIYY242 pKa = 10.41LDD244 pKa = 4.48DD245 pKa = 4.52NGAGMGFTNAVISGNTLIDD264 pKa = 3.44GTGQGVLLEE273 pKa = 4.45NVTGTVSNNTLIWSGTGTQANNSPRR298 pKa = 11.84FDD300 pKa = 3.37INAGSDD306 pKa = 3.62HH307 pKa = 7.5INFTDD312 pKa = 3.21NTGNVSIQTGSTFLNFVRR330 pKa = 11.84QTGMIAEE337 pKa = 4.73DD338 pKa = 3.55ATMSSADD345 pKa = 4.3RR346 pKa = 11.84DD347 pKa = 4.45TITIDD352 pKa = 3.26YY353 pKa = 9.3MVITAHH359 pKa = 6.92NSYY362 pKa = 10.85ALNATTSDD370 pKa = 4.23LYY372 pKa = 10.88FQGGIGDD379 pKa = 4.07FVGTGNTLANHH390 pKa = 6.4IVGSTGNDD398 pKa = 3.04TLTGNGGADD407 pKa = 3.51YY408 pKa = 11.49LEE410 pKa = 4.78GKK412 pKa = 7.95TGNDD416 pKa = 3.16TYY418 pKa = 11.75VVDD421 pKa = 4.27NLSQVIYY428 pKa = 10.59DD429 pKa = 3.52SGGTEE434 pKa = 4.02LVKK437 pKa = 11.04SKK439 pKa = 10.68ISWTLQTGLEE449 pKa = 4.06NLTFIGTGSATLAGNAAPNIIIGGGGADD477 pKa = 3.26RR478 pKa = 11.84LIGNGGADD486 pKa = 3.63TLQGGLGNDD495 pKa = 3.57TYY497 pKa = 11.63VIDD500 pKa = 4.74NLGQKK505 pKa = 8.24ITDD508 pKa = 3.44TGGTDD513 pKa = 3.47TIEE516 pKa = 5.17ASLNWTLQSGIEE528 pKa = 3.97NLTLTGAAVFATGNTSANTLRR549 pKa = 11.84GNGLNNTLDD558 pKa = 3.83GKK560 pKa = 9.2TGADD564 pKa = 3.22SMYY567 pKa = 10.73GGAGDD572 pKa = 5.69DD573 pKa = 4.26IYY575 pKa = 10.98IVDD578 pKa = 3.71NAGDD582 pKa = 3.54KK583 pKa = 10.7VFEE586 pKa = 4.32VVAGIDD592 pKa = 3.11SGGFDD597 pKa = 3.57TVKK600 pKa = 9.88TALASWTMAAGVEE613 pKa = 4.27VLTYY617 pKa = 9.46TGSAAFTGTGNTLNNTISGGAAIDD641 pKa = 3.89TLNGLAGDD649 pKa = 4.76DD650 pKa = 4.1KK651 pKa = 11.67LIGNAGDD658 pKa = 4.06DD659 pKa = 4.56KK660 pKa = 11.46INGGAGSDD668 pKa = 3.65TLTGGTGNDD677 pKa = 2.87TFIFYY682 pKa = 10.53RR683 pKa = 11.84GEE685 pKa = 4.18AYY687 pKa = 10.45SDD689 pKa = 3.98SITDD693 pKa = 3.51FNGRR697 pKa = 11.84ISTVGDD703 pKa = 3.8SIEE706 pKa = 3.82LRR708 pKa = 11.84GWGTGSTFTNIAGTNFWVIHH728 pKa = 7.01DD729 pKa = 4.28GLDD732 pKa = 3.47GYY734 pKa = 10.45EE735 pKa = 4.27EE736 pKa = 4.7YY737 pKa = 10.53ISVTGGVDD745 pKa = 3.2PSDD748 pKa = 3.07WSFTQSVTSVVAPAAAVMPMSVLALDD774 pKa = 3.9TQGVDD779 pKa = 3.64LTSVAATEE787 pKa = 4.18TDD789 pKa = 3.69LVLATSDD796 pKa = 3.81TPDD799 pKa = 3.5LSSVLDD805 pKa = 4.15DD806 pKa = 5.72SVDD809 pKa = 3.63QTDD812 pKa = 3.9MFPDD816 pKa = 4.58DD817 pKa = 3.93SAQAAQQVTEE827 pKa = 4.43DD828 pKa = 4.21AGTFDD833 pKa = 3.94PAVPEE838 pKa = 3.75AAAAQGHH845 pKa = 6.6DD846 pKa = 3.6GGRR849 pKa = 11.84DD850 pKa = 3.98GVWLPFANADD860 pKa = 3.25HH861 pKa = 6.53HH862 pKa = 6.95VPYY865 pKa = 10.52HH866 pKa = 5.77HH867 pKa = 7.39AA868 pKa = 4.16

Molecular weight: 89.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9EN67|A0A4Y9EN67_9SPHN Replication-associated recombination protein A OS=Polymorphobacter arshaanensis OX=2511025 GN=EUV02_09930 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.36GFRR19 pKa = 11.84TRR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.01VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.45GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3035

0

3035

983693

28

3624

324.1

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.307 ± 0.065

0.763 ± 0.014

5.832 ± 0.034

4.551 ± 0.042

3.605 ± 0.023

8.996 ± 0.052

2.012 ± 0.022

4.946 ± 0.032

3.087 ± 0.036

9.958 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.022

2.742 ± 0.035

5.372 ± 0.035

2.865 ± 0.021

6.535 ± 0.057

4.958 ± 0.029

5.719 ± 0.042

7.57 ± 0.035

1.468 ± 0.019

2.261 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski