Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Micrococcus; Micrococcus luteus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5C7D8|C5C7D8_MICLC Uncharacterized protein OS=Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) OX=465515 GN=Mlut_21580 PE=4 SV=1
MM1 pKa = 7.69ALFSQPVFINLPTTDD16 pKa = 3.4TEE18 pKa = 4.6RR19 pKa = 11.84IRR21 pKa = 11.84EE22 pKa = 3.94FWTTLGATAMDD33 pKa = 5.22DD34 pKa = 3.72YY35 pKa = 11.53SDD37 pKa = 3.66EE38 pKa = 4.43HH39 pKa = 6.73SVCIEE44 pKa = 4.05LTEE47 pKa = 4.23STYY50 pKa = 11.85AMYY53 pKa = 10.73LSPGYY58 pKa = 8.9YY59 pKa = 9.32TDD61 pKa = 5.51FIGNRR66 pKa = 11.84EE67 pKa = 3.97IADD70 pKa = 3.86CLTTNSALMALTVGDD85 pKa = 4.06AAAVDD90 pKa = 4.0ALADD94 pKa = 3.97RR95 pKa = 11.84AIEE98 pKa = 4.09LGAAEE103 pKa = 4.44VPLPEE108 pKa = 4.87DD109 pKa = 3.74LRR111 pKa = 11.84EE112 pKa = 3.9EE113 pKa = 4.14MADD116 pKa = 3.12SGMHH120 pKa = 5.39SRR122 pKa = 11.84EE123 pKa = 3.67IVDD126 pKa = 3.61PDD128 pKa = 3.06GHH130 pKa = 5.02QWEE133 pKa = 5.71FITAA137 pKa = 3.95

Molecular weight: 15.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5CCU0|C5CCU0_MICLC Uncharacterized protein OS=Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) OX=465515 GN=Mlut_18090 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2207

0

2207

731209

32

1550

331.3

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.509 ± 0.082

0.521 ± 0.012

6.211 ± 0.047

6.195 ± 0.056

2.676 ± 0.03

9.183 ± 0.043

2.334 ± 0.027

3.237 ± 0.041

1.78 ± 0.038

10.142 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.02

1.565 ± 0.03

5.875 ± 0.042

2.823 ± 0.026

8.022 ± 0.065

4.623 ± 0.035

5.95 ± 0.038

9.235 ± 0.045

1.494 ± 0.025

1.712 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski