Tolypothrix bouteillei VB521301

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Tolypothrichaceae; Tolypothrix; Tolypothrix bouteillei

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C1QYQ7|A0A0C1QYQ7_9CYAN Urease accessory protein UreF OS=Tolypothrix bouteillei VB521301 OX=1479485 GN=ureF PE=3 SV=1
MM1 pKa = 7.87EE2 pKa = 4.26IQFRR6 pKa = 11.84EE7 pKa = 3.67IDD9 pKa = 4.12PFNVWIWLKK18 pKa = 10.74FSTVPSRR25 pKa = 11.84QEE27 pKa = 3.21RR28 pKa = 11.84QYY30 pKa = 10.75IEE32 pKa = 4.56EE33 pKa = 4.48VFDD36 pKa = 3.1SWFYY40 pKa = 10.95LGKK43 pKa = 10.68LGGFNAEE50 pKa = 3.91NLQVQEE56 pKa = 4.17TGVNLSYY63 pKa = 10.29MDD65 pKa = 4.38YY66 pKa = 11.1DD67 pKa = 3.59SEE69 pKa = 6.01GYY71 pKa = 9.9DD72 pKa = 3.18KK73 pKa = 11.83SMLALMHH80 pKa = 6.36NMGEE84 pKa = 4.21VEE86 pKa = 5.4YY87 pKa = 9.83EE88 pKa = 4.33GTWARR93 pKa = 11.84CWFDD97 pKa = 4.96LGTADD102 pKa = 6.27AIALDD107 pKa = 3.68ILINALKK114 pKa = 10.04QLSAEE119 pKa = 4.2YY120 pKa = 9.4VTIEE124 pKa = 3.58QLYY127 pKa = 10.33IGGEE131 pKa = 4.03NEE133 pKa = 3.99DD134 pKa = 3.76WPVEE138 pKa = 3.94NSDD141 pKa = 3.83NLNSFVFDD149 pKa = 3.59NN150 pKa = 4.19

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1R2N5|A0A0C1R2N5_9CYAN ABC transporter ATP-binding protein OS=Tolypothrix bouteillei VB521301 OX=1479485 GN=DA73_0213770 PE=4 SV=1
PP1 pKa = 7.42GGAKK5 pKa = 10.06VPSDD9 pKa = 3.28VTLLVEE15 pKa = 5.03SGPLLQMIPPGVLYY29 pKa = 10.65SVLLFRR35 pKa = 11.84VHH37 pKa = 6.48AVAIVRR43 pKa = 11.84AQLVPSPRR51 pKa = 11.84YY52 pKa = 9.17VPPLAAHH59 pKa = 6.1CVGRR63 pKa = 11.84STTQLCGVRR72 pKa = 11.84FGRR75 pKa = 11.84QQAPNDD81 pKa = 3.88PVGASAICSWRR92 pKa = 11.84RR93 pKa = 11.84MLVVSGAGGVPPWAVIFAKK112 pKa = 10.54LNKK115 pKa = 9.84LWAQYY120 pKa = 11.17NFVRR124 pKa = 11.84VDD126 pKa = 4.01FSSS129 pKa = 2.91

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7749

6

7755

2420647

31

4999

312.1

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.97 ± 0.03

1.004 ± 0.011

4.763 ± 0.022

6.348 ± 0.026

3.995 ± 0.018

6.599 ± 0.03

1.844 ± 0.017

6.579 ± 0.021

4.964 ± 0.027

10.949 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.012

4.371 ± 0.027

4.623 ± 0.023

5.248 ± 0.026

5.27 ± 0.022

6.626 ± 0.024

5.735 ± 0.022

6.813 ± 0.023

1.423 ± 0.012

3.07 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski