Vibrio phage pYD21-A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QC66|M4QC66_9CAUD Uncharacterized protein OS=Vibrio phage pYD21-A OX=754049 GN=VPKG_00072 PE=4 SV=1
MM1 pKa = 7.58DD2 pKa = 4.64WSWIDD7 pKa = 3.24MTATTEE13 pKa = 3.76PKK15 pKa = 10.12YY16 pKa = 10.83FVIGPVLEE24 pKa = 4.79TINNAMLDD32 pKa = 3.81EE33 pKa = 4.62MEE35 pKa = 4.67PPYY38 pKa = 10.33KK39 pKa = 9.6IGMSSSVKK47 pKa = 9.83EE48 pKa = 4.29LYY50 pKa = 10.45DD51 pKa = 4.32SEE53 pKa = 4.63VEE55 pKa = 4.41QMKK58 pKa = 10.25HH59 pKa = 6.19DD60 pKa = 3.97KK61 pKa = 11.44AFMQCLGYY69 pKa = 10.7VDD71 pKa = 5.4IIVDD75 pKa = 4.87DD76 pKa = 4.55EE77 pKa = 4.54LDD79 pKa = 3.5SDD81 pKa = 4.75GVYY84 pKa = 11.09LLAA87 pKa = 6.46

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QC60|M4QC60_9CAUD Uncharacterized protein OS=Vibrio phage pYD21-A OX=754049 GN=VPKG_00067 PE=4 SV=1
MM1 pKa = 6.55QTQNNVRR8 pKa = 11.84TTNIEE13 pKa = 3.72VATIKK18 pKa = 10.86DD19 pKa = 3.34AMAFNYY25 pKa = 9.95GNQRR29 pKa = 11.84GMAKK33 pKa = 10.16HH34 pKa = 6.33LGINRR39 pKa = 11.84GTLAKK44 pKa = 10.24KK45 pKa = 9.78LASNRR50 pKa = 11.84DD51 pKa = 3.39YY52 pKa = 10.79YY53 pKa = 11.51VRR55 pKa = 11.84VHH57 pKa = 6.92RR58 pKa = 11.84DD59 pKa = 2.36GWNNITHH66 pKa = 6.91LEE68 pKa = 4.11WLNKK72 pKa = 9.64

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14180

49

1077

191.6

21.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.173 ± 0.446

1.403 ± 0.173

6.312 ± 0.234

7.073 ± 0.316

3.568 ± 0.137

7.32 ± 0.286

2.052 ± 0.242

5.994 ± 0.212

6.354 ± 0.403

7.292 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.231

5.176 ± 0.26

3.66 ± 0.181

3.505 ± 0.162

4.669 ± 0.21

6.608 ± 0.232

6.058 ± 0.319

6.7 ± 0.237

1.382 ± 0.1

3.843 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski