Beihai astro-like virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KNT1|A0A1L3KNT1_9VIRU Putative capsid OS=Beihai astro-like virus OX=1922353 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 4.98KK3 pKa = 9.65TPKK6 pKa = 9.07EE7 pKa = 3.79QRR9 pKa = 11.84TPRR12 pKa = 11.84VKK14 pKa = 10.37KK15 pKa = 9.36QQPQKK20 pKa = 10.86KK21 pKa = 8.83PAGQQQPRR29 pKa = 11.84KK30 pKa = 8.66PAQQKK35 pKa = 9.51RR36 pKa = 11.84RR37 pKa = 11.84SPMPQKK43 pKa = 9.18THH45 pKa = 6.97DD46 pKa = 3.67GEE48 pKa = 4.8IYY50 pKa = 10.41SSSSLVSIIHH60 pKa = 6.6GNQDD64 pKa = 4.01DD65 pKa = 4.42FTTPLAISLNPLVVAEE81 pKa = 4.17EE82 pKa = 4.18AKK84 pKa = 11.04DD85 pKa = 3.71PILNMKK91 pKa = 9.47CMSYY95 pKa = 11.13NCFKK99 pKa = 10.98VLSAKK104 pKa = 10.73LEE106 pKa = 4.18LKK108 pKa = 10.19AVCGPNAAVGTTATVGSWQNVDD130 pKa = 5.05DD131 pKa = 4.19PTGPQTPNTALKK143 pKa = 8.98MDD145 pKa = 4.11RR146 pKa = 11.84AVTVGIGMRR155 pKa = 11.84ACAPIKK161 pKa = 10.62HH162 pKa = 5.99SDD164 pKa = 3.55EE165 pKa = 4.34VKK167 pKa = 10.52VVYY170 pKa = 10.06TEE172 pKa = 3.79ATAAPRR178 pKa = 11.84TWNPGHH184 pKa = 7.12AIVSLIGQTGTVYY197 pKa = 10.6NQEE200 pKa = 3.84QTPVWKK206 pKa = 10.37RR207 pKa = 11.84PLWHH211 pKa = 7.05AFLHH215 pKa = 4.81YY216 pKa = 8.86TYY218 pKa = 10.58HH219 pKa = 5.56FTSPKK224 pKa = 10.12DD225 pKa = 3.39NRR227 pKa = 11.84NRR229 pKa = 11.84GSLAAGDD236 pKa = 5.09LDD238 pKa = 3.84DD239 pKa = 5.1QKK241 pKa = 11.71VSLHH245 pKa = 6.72EE246 pKa = 4.62IPDD249 pKa = 3.68EE250 pKa = 4.14PLVLEE255 pKa = 4.81MPNSPAARR263 pKa = 11.84KK264 pKa = 8.98LICRR268 pKa = 11.84GRR270 pKa = 11.84KK271 pKa = 7.69AQGRR275 pKa = 11.84GRR277 pKa = 11.84AGLWDD282 pKa = 4.16FASGILQGLSGVLPPPWGSLLNAGVALVRR311 pKa = 11.84PKK313 pKa = 10.08TATTRR318 pKa = 11.84EE319 pKa = 3.82GDD321 pKa = 3.87TIQLEE326 pKa = 4.39LFQTLSDD333 pKa = 3.88VAQDD337 pKa = 3.62QPVMGVEE344 pKa = 3.93ARR346 pKa = 11.84TTQMDD351 pKa = 5.76LINGHH356 pKa = 5.25YY357 pKa = 9.1TQMTDD362 pKa = 2.9PGANSVLEE370 pKa = 4.53DD371 pKa = 3.53SDD373 pKa = 4.05GGGGVLPPTGGGIVWHH389 pKa = 7.2DD390 pKa = 4.45ANPDD394 pKa = 3.32DD395 pKa = 4.6WSGDD399 pKa = 3.25EE400 pKa = 3.97QIIVNSYY407 pKa = 7.46VNKK410 pKa = 10.53YY411 pKa = 9.55ADD413 pKa = 3.21NYY415 pKa = 9.48FNEE418 pKa = 4.45VYY420 pKa = 9.87PVYY423 pKa = 10.68SSSDD427 pKa = 3.25VNYY430 pKa = 10.6NITVTKK436 pKa = 10.68GPLAGNNYY444 pKa = 8.69QFYY447 pKa = 10.46HH448 pKa = 7.71AMEE451 pKa = 4.18FQKK454 pKa = 10.16WKK456 pKa = 9.4HH457 pKa = 5.26QNTEE461 pKa = 3.81VTAVWVRR468 pKa = 11.84FQKK471 pKa = 9.98MSNMDD476 pKa = 3.95LQAKK480 pKa = 8.63GYY482 pKa = 10.4QMYY485 pKa = 10.5LPQFIEE491 pKa = 4.13KK492 pKa = 8.38FTNASSTSGEE502 pKa = 4.17EE503 pKa = 3.99KK504 pKa = 10.44FVMLAKK510 pKa = 10.73DD511 pKa = 3.5GGRR514 pKa = 11.84VQLQSKK520 pKa = 10.12SSPTVFTRR528 pKa = 11.84FLNMNSSTPWSALFLGEE545 pKa = 4.06SRR547 pKa = 11.84SIKK550 pKa = 9.84IYY552 pKa = 10.56RR553 pKa = 11.84DD554 pKa = 3.59GTSLAAGNWNPCQDD568 pKa = 3.78VLPGNNLSNLQATTAAAGDD587 pKa = 3.98EE588 pKa = 4.43EE589 pKa = 4.37EE590 pKa = 5.56AEE592 pKa = 4.05ADD594 pKa = 5.13KK595 pKa = 11.4EE596 pKa = 4.13DD597 pKa = 3.74LLDD600 pKa = 4.95RR601 pKa = 11.84IRR603 pKa = 11.84YY604 pKa = 8.77LEE606 pKa = 4.45RR607 pKa = 11.84EE608 pKa = 4.37LEE610 pKa = 4.2SAEE613 pKa = 4.14TEE615 pKa = 4.21LMVSRR620 pKa = 11.84GVGWDD625 pKa = 3.89GSCPVV630 pKa = 3.74

Molecular weight: 69.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNQ2|A0A1L3KNQ2_9VIRU RNA-dependent RNA polymerase OS=Beihai astro-like virus OX=1922353 PE=4 SV=1
MM1 pKa = 8.11SGMLLKK7 pKa = 10.42VLCSVMLLSLSMKK20 pKa = 9.23TDD22 pKa = 4.63AILVVGTCGQVNLPPVATAATAPLMHH48 pKa = 7.16LCTTTNCSVNCDD60 pKa = 3.45VYY62 pKa = 11.25RR63 pKa = 11.84DD64 pKa = 3.74ATRR67 pKa = 11.84YY68 pKa = 8.24PAEE71 pKa = 4.15EE72 pKa = 4.27NEE74 pKa = 4.15LEE76 pKa = 4.07SRR78 pKa = 11.84RR79 pKa = 11.84VRR81 pKa = 11.84RR82 pKa = 11.84HH83 pKa = 5.26CLMGFRR89 pKa = 11.84MNNTYY94 pKa = 10.91GEE96 pKa = 4.48YY97 pKa = 11.06GQFTPPGLSAMTTYY111 pKa = 11.25GNRR114 pKa = 11.84FGLIAPLNQTKK125 pKa = 8.84WEE127 pKa = 3.96NMIIALGGLHH137 pKa = 6.34NCPRR141 pKa = 11.84DD142 pKa = 3.28PTTAMNAAPTPEE154 pKa = 4.06EE155 pKa = 3.84TSAYY159 pKa = 10.36DD160 pKa = 3.64AFHH163 pKa = 6.69KK164 pKa = 9.88TLDD167 pKa = 4.84AIWAHH172 pKa = 5.32YY173 pKa = 10.67NRR175 pKa = 11.84FFSEE179 pKa = 3.92EE180 pKa = 3.72ARR182 pKa = 11.84AKK184 pKa = 10.31AHH186 pKa = 5.04QQRR189 pKa = 11.84LYY191 pKa = 10.4PEE193 pKa = 4.38RR194 pKa = 11.84LEE196 pKa = 4.19HH197 pKa = 6.31LWYY200 pKa = 9.4VQLMMVLSAWWQTLVDD216 pKa = 3.42WHH218 pKa = 6.56IVSAAVMSTFVLTKK232 pKa = 9.93IYY234 pKa = 9.97GQSTTTPWYY243 pKa = 10.24RR244 pKa = 11.84IIALIPAMFTKK255 pKa = 10.62GGLGLALLFTPFVNDD270 pKa = 3.28FNGVLLPMLYY280 pKa = 10.61LLSHH284 pKa = 6.73YY285 pKa = 10.27IYY287 pKa = 10.08LTGGCILIAITLNVGYY303 pKa = 10.98LMGRR307 pKa = 11.84WIVPEE312 pKa = 4.15EE313 pKa = 4.16KK314 pKa = 10.14EE315 pKa = 4.22QDD317 pKa = 3.57GATTNVDD324 pKa = 4.12FMVTTVHH331 pKa = 6.26TARR334 pKa = 11.84SLALVIVACVCGLLATLTPGFPFVLTVMVAISVALILPDD373 pKa = 4.27PPHH376 pKa = 6.48EE377 pKa = 4.44TTAHH381 pKa = 4.86VMVGNGPRR389 pKa = 11.84ARR391 pKa = 11.84RR392 pKa = 11.84VPVKK396 pKa = 10.74VYY398 pKa = 7.3TQPGSRR404 pKa = 11.84NNRR407 pKa = 11.84FWYY410 pKa = 9.76QLQANKK416 pKa = 9.95RR417 pKa = 11.84VQRR420 pKa = 11.84LPEE423 pKa = 3.91TAFGATLTVSQGDD436 pKa = 4.11AISTGFICRR445 pKa = 11.84GMVFTIAHH453 pKa = 5.39GTKK456 pKa = 8.47PTNEE460 pKa = 3.67GMEE463 pKa = 4.03VKK465 pKa = 10.39GPNKK469 pKa = 9.98IVFKK473 pKa = 10.39PGAYY477 pKa = 9.11VGKK480 pKa = 10.19IKK482 pKa = 10.7LSEE485 pKa = 4.25DD486 pKa = 3.17QAVYY490 pKa = 10.7AFKK493 pKa = 11.01LPTEE497 pKa = 4.34ASRR500 pKa = 11.84LKK502 pKa = 10.11SLPVATEE509 pKa = 3.86IKK511 pKa = 10.05EE512 pKa = 4.13GWHH515 pKa = 4.19TVIYY519 pKa = 10.46RR520 pKa = 11.84NEE522 pKa = 3.93DD523 pKa = 3.59CACAGEE529 pKa = 4.23MFYY532 pKa = 11.5GNWEE536 pKa = 4.1DD537 pKa = 4.25DD538 pKa = 4.05EE539 pKa = 5.54LRR541 pKa = 11.84GSYY544 pKa = 10.01TNARR548 pKa = 11.84GYY550 pKa = 10.43SGSPIVNSDD559 pKa = 3.22GKK561 pKa = 9.15ITAVHH566 pKa = 6.4CGGIGEE572 pKa = 4.52VGVSYY577 pKa = 10.94RR578 pKa = 11.84FTAQMIEE585 pKa = 3.95NMTKK589 pKa = 8.98TDD591 pKa = 3.49KK592 pKa = 10.65PKK594 pKa = 9.42KK595 pKa = 8.63TEE597 pKa = 4.0EE598 pKa = 4.03QVEE601 pKa = 4.23DD602 pKa = 3.55QALNPLKK609 pKa = 10.37EE610 pKa = 3.89LALALEE616 pKa = 4.41RR617 pKa = 11.84MTAQFLRR624 pKa = 11.84LEE626 pKa = 4.21AQVRR630 pKa = 11.84DD631 pKa = 3.79NEE633 pKa = 4.33AKK635 pKa = 8.85NTEE638 pKa = 4.06RR639 pKa = 11.84LDD641 pKa = 3.68EE642 pKa = 4.26VEE644 pKa = 5.11NMVNQKK650 pKa = 10.53KK651 pKa = 8.04MFTDD655 pKa = 3.86DD656 pKa = 3.35EE657 pKa = 4.71YY658 pKa = 11.27EE659 pKa = 4.16YY660 pKa = 10.38LTGRR664 pKa = 11.84RR665 pKa = 11.84GMSRR669 pKa = 11.84DD670 pKa = 3.14KK671 pKa = 10.41MRR673 pKa = 11.84EE674 pKa = 3.37IAKK677 pKa = 10.09RR678 pKa = 11.84RR679 pKa = 11.84IAEE682 pKa = 3.87RR683 pKa = 11.84DD684 pKa = 3.17YY685 pKa = 11.8DD686 pKa = 4.06EE687 pKa = 6.44QNDD690 pKa = 3.86QAKK693 pKa = 9.91KK694 pKa = 10.3KK695 pKa = 9.06PDD697 pKa = 3.43VVVASPGALPAIQPTPYY714 pKa = 9.32PAYY717 pKa = 9.62PVVTGKK723 pKa = 10.52APKK726 pKa = 10.1GKK728 pKa = 9.63RR729 pKa = 11.84LHH731 pKa = 5.13EE732 pKa = 4.38WKK734 pKa = 10.52VGMVYY739 pKa = 10.25RR740 pKa = 11.84PNTRR744 pKa = 11.84DD745 pKa = 3.06VHH747 pKa = 7.96VGEE750 pKa = 4.58KK751 pKa = 10.33CCNEE755 pKa = 3.84VVAGYY760 pKa = 10.24EE761 pKa = 4.16NEE763 pKa = 4.47PDD765 pKa = 3.56TQTSHH770 pKa = 7.07IVLTLCTVKK779 pKa = 10.68LVGAGDD785 pKa = 3.65VAYY788 pKa = 10.56CPACEE793 pKa = 3.95PDD795 pKa = 3.22QLRR798 pKa = 11.84FEE800 pKa = 4.61NQRR803 pKa = 11.84GHH805 pKa = 6.89GPLKK809 pKa = 9.06QTKK812 pKa = 8.94CGQKK816 pKa = 6.5TTSMPGHH823 pKa = 6.09VMHH826 pKa = 7.15CKK828 pKa = 8.95ATPKK832 pKa = 9.62CTEE835 pKa = 4.11SDD837 pKa = 4.2CINAPICTDD846 pKa = 3.33HH847 pKa = 6.95CPHH850 pKa = 6.31YY851 pKa = 10.49NCEE854 pKa = 3.85KK855 pKa = 10.25VRR857 pKa = 11.84RR858 pKa = 11.84EE859 pKa = 3.85DD860 pKa = 4.95GIATEE865 pKa = 5.09CPGAKK870 pKa = 9.65CKK872 pKa = 10.49NDD874 pKa = 3.25YY875 pKa = 10.25CKK877 pKa = 10.78KK878 pKa = 10.51KK879 pKa = 10.85GFLKK883 pKa = 10.93GG884 pKa = 3.29

Molecular weight: 98.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1905

391

884

635.0

70.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.717 ± 0.449

2.362 ± 0.609

4.829 ± 0.467

6.194 ± 0.416

2.835 ± 0.131

7.192 ± 0.333

2.415 ± 0.226

3.937 ± 0.201

5.722 ± 0.075

7.979 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.255 ± 0.26

4.829 ± 0.253

5.932 ± 0.374

4.094 ± 0.785

5.249 ± 0.406

4.987 ± 0.945

7.717 ± 0.407

7.139 ± 0.341

1.68 ± 0.2

3.937 ± 0.53
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski