Amino acid dipepetide frequency for Actinobacillus seminis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.967AlaAla: 6.967 ± 0.135
0.932AlaCys: 0.932 ± 0.042
4.516AlaAsp: 4.516 ± 0.099
5.942AlaGlu: 5.942 ± 0.128
3.349AlaPhe: 3.349 ± 0.09
5.909AlaGly: 5.909 ± 0.12
1.466AlaHis: 1.466 ± 0.045
6.325AlaIle: 6.325 ± 0.116
5.966AlaLys: 5.966 ± 0.151
10.17AlaLeu: 10.17 ± 0.129
2.235AlaMet: 2.235 ± 0.072
3.819AlaAsn: 3.819 ± 0.106
2.498AlaPro: 2.498 ± 0.067
4.371AlaGln: 4.371 ± 0.097
3.461AlaArg: 3.461 ± 0.097
3.896AlaSer: 3.896 ± 0.095
4.571AlaThr: 4.571 ± 0.115
6.212AlaVal: 6.212 ± 0.122
0.88AlaTrp: 0.88 ± 0.039
2.374AlaTyr: 2.374 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.842CysAla: 0.842 ± 0.04
0.139CysCys: 0.139 ± 0.017
0.567CysAsp: 0.567 ± 0.032
0.619CysGlu: 0.619 ± 0.036
0.5CysPhe: 0.5 ± 0.032
0.99CysGly: 0.99 ± 0.042
0.315CysHis: 0.315 ± 0.025
0.594CysIle: 0.594 ± 0.032
0.438CysLys: 0.438 ± 0.025
1.034CysLeu: 1.034 ± 0.042
0.172CysMet: 0.172 ± 0.019
0.35CysAsn: 0.35 ± 0.029
0.443CysPro: 0.443 ± 0.029
0.482CysGln: 0.482 ± 0.03
0.515CysArg: 0.515 ± 0.03
0.66CysSer: 0.66 ± 0.035
0.459CysThr: 0.459 ± 0.03
0.626CysVal: 0.626 ± 0.03
0.131CysTrp: 0.131 ± 0.015
0.394CysTyr: 0.394 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.595AspAla: 3.595 ± 0.098
0.577AspCys: 0.577 ± 0.032
2.416AspAsp: 2.416 ± 0.074
3.534AspGlu: 3.534 ± 0.098
2.539AspPhe: 2.539 ± 0.068
3.543AspGly: 3.543 ± 0.147
0.991AspHis: 0.991 ± 0.053
3.789AspIle: 3.789 ± 0.078
3.535AspLys: 3.535 ± 0.127
5.225AspLeu: 5.225 ± 0.109
1.181AspMet: 1.181 ± 0.043
2.538AspAsn: 2.538 ± 0.092
2.145AspPro: 2.145 ± 0.061
1.74AspGln: 1.74 ± 0.049
2.257AspArg: 2.257 ± 0.067
2.527AspSer: 2.527 ± 0.081
2.293AspThr: 2.293 ± 0.083
3.476AspVal: 3.476 ± 0.066
0.799AspTrp: 0.799 ± 0.038
2.082AspTyr: 2.082 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
4.642GluAla: 4.642 ± 0.104
0.44GluCys: 0.44 ± 0.027
2.746GluAsp: 2.746 ± 0.069
3.676GluGlu: 3.676 ± 0.11
2.347GluPhe: 2.347 ± 0.067
3.173GluGly: 3.173 ± 0.079
1.24GluHis: 1.24 ± 0.045
4.721GluIle: 4.721 ± 0.107
5.06GluLys: 5.06 ± 0.098
6.031GluLeu: 6.031 ± 0.1
1.759GluMet: 1.759 ± 0.058
3.726GluAsn: 3.726 ± 0.078
1.658GluPro: 1.658 ± 0.051
3.895GluGln: 3.895 ± 0.094
3.19GluArg: 3.19 ± 0.079
2.81GluSer: 2.81 ± 0.063
3.069GluThr: 3.069 ± 0.073
4.004GluVal: 4.004 ± 0.094
0.681GluTrp: 0.681 ± 0.036
1.8GluTyr: 1.8 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
3.807PheAla: 3.807 ± 0.079
0.599PheCys: 0.599 ± 0.031
2.607PheAsp: 2.607 ± 0.074
2.35PheGlu: 2.35 ± 0.072
1.936PhePhe: 1.936 ± 0.073
3.291PheGly: 3.291 ± 0.083
0.864PheHis: 0.864 ± 0.04
3.025PheIle: 3.025 ± 0.09
2.196PheLys: 2.196 ± 0.067
3.896PheLeu: 3.896 ± 0.098
1.037PheMet: 1.037 ± 0.046
2.152PheAsn: 2.152 ± 0.056
1.408PhePro: 1.408 ± 0.051
1.302PheGln: 1.302 ± 0.047
1.573PheArg: 1.573 ± 0.053
3.308PheSer: 3.308 ± 0.093
2.319PheThr: 2.319 ± 0.054
2.899PheVal: 2.899 ± 0.078
0.525PheTrp: 0.525 ± 0.029
1.515PheTyr: 1.515 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
5.364GlyAla: 5.364 ± 0.138
0.801GlyCys: 0.801 ± 0.04
3.332GlyAsp: 3.332 ± 0.099
4.462GlyGlu: 4.462 ± 0.103
3.133GlyPhe: 3.133 ± 0.079
4.736GlyGly: 4.736 ± 0.121
1.302GlyHis: 1.302 ± 0.046
5.365GlyIle: 5.365 ± 0.092
5.258GlyLys: 5.258 ± 0.135
6.968GlyLeu: 6.968 ± 0.13
1.884GlyMet: 1.884 ± 0.062
3.403GlyAsn: 3.403 ± 0.172
1.247GlyPro: 1.247 ± 0.043
2.621GlyGln: 2.621 ± 0.068
2.96GlyArg: 2.96 ± 0.07
3.948GlySer: 3.948 ± 0.125
3.569GlyThr: 3.569 ± 0.121
5.038GlyVal: 5.038 ± 0.094
0.914GlyTrp: 0.914 ± 0.052
2.618GlyTyr: 2.618 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
1.297HisAla: 1.297 ± 0.046
0.4HisCys: 0.4 ± 0.025
0.894HisAsp: 0.894 ± 0.054
0.908HisGlu: 0.908 ± 0.04
1.229HisPhe: 1.229 ± 0.044
1.322HisGly: 1.322 ± 0.051
0.684HisHis: 0.684 ± 0.046
1.608HisIle: 1.608 ± 0.059
1.08HisLys: 1.08 ± 0.044
2.284HisLeu: 2.284 ± 0.064
0.399HisMet: 0.399 ± 0.027
0.935HisAsn: 0.935 ± 0.039
1.039HisPro: 1.039 ± 0.048
1.292HisGln: 1.292 ± 0.045
1.007HisArg: 1.007 ± 0.041
1.318HisSer: 1.318 ± 0.044
0.955HisThr: 0.955 ± 0.04
0.971HisVal: 0.971 ± 0.039
0.314HisTrp: 0.314 ± 0.025
0.894HisTyr: 0.894 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
6.882IleAla: 6.882 ± 0.099
0.779IleCys: 0.779 ± 0.036
3.994IleAsp: 3.994 ± 0.082
4.527IleGlu: 4.527 ± 0.096
2.867IlePhe: 2.867 ± 0.072
5.599IleGly: 5.599 ± 0.12
1.31IleHis: 1.31 ± 0.05
4.587IleIle: 4.587 ± 0.109
3.868IleLys: 3.868 ± 0.089
6.339IleLeu: 6.339 ± 0.133
1.322IleMet: 1.322 ± 0.05
3.296IleAsn: 3.296 ± 0.07
2.795IlePro: 2.795 ± 0.072
2.639IleGln: 2.639 ± 0.075
2.976IleArg: 2.976 ± 0.068
4.681IleSer: 4.681 ± 0.091
3.832IleThr: 3.832 ± 0.093
4.394IleVal: 4.394 ± 0.092
0.671IleTrp: 0.671 ± 0.034
2.062IleTyr: 2.062 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
5.681LysAla: 5.681 ± 0.148
0.416LysCys: 0.416 ± 0.026
3.031LysAsp: 3.031 ± 0.135
3.704LysGlu: 3.704 ± 0.101
2.051LysPhe: 2.051 ± 0.064
3.97LysGly: 3.97 ± 0.121
1.091LysHis: 1.091 ± 0.046
4.333LysIle: 4.333 ± 0.103
4.018LysLys: 4.018 ± 0.106
6.035LysLeu: 6.035 ± 0.099
1.809LysMet: 1.809 ± 0.055
3.515LysAsn: 3.515 ± 0.122
2.341LysPro: 2.341 ± 0.066
3.239LysGln: 3.239 ± 0.076
2.919LysArg: 2.919 ± 0.077
3.493LysSer: 3.493 ± 0.094
3.591LysThr: 3.591 ± 0.102
4.169LysVal: 4.169 ± 0.085
0.582LysTrp: 0.582 ± 0.034
1.74LysTyr: 1.74 ± 0.067
0.0LysXaa: 0.0 ± 0.0
Leu
9.648LeuAla: 9.648 ± 0.153
1.113LeuCys: 1.113 ± 0.045
5.358LeuAsp: 5.358 ± 0.098
5.644LeuGlu: 5.644 ± 0.106
4.456LeuPhe: 4.456 ± 0.118
7.331LeuGly: 7.331 ± 0.121
2.074LeuHis: 2.074 ± 0.062
6.622LeuIle: 6.622 ± 0.139
5.964LeuLys: 5.964 ± 0.114
10.553LeuLeu: 10.553 ± 0.24
2.402LeuMet: 2.402 ± 0.069
5.001LeuAsn: 5.001 ± 0.086
4.697LeuPro: 4.697 ± 0.11
4.759LeuGln: 4.759 ± 0.105
4.719LeuArg: 4.719 ± 0.102
7.318LeuSer: 7.318 ± 0.13
5.997LeuThr: 5.997 ± 0.108
6.421LeuVal: 6.421 ± 0.117
1.086LeuTrp: 1.086 ± 0.037
2.702LeuTyr: 2.702 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
2.361MetAla: 2.361 ± 0.067
0.214MetCys: 0.214 ± 0.018
0.96MetAsp: 0.96 ± 0.036
1.147MetGlu: 1.147 ± 0.044
0.805MetPhe: 0.805 ± 0.034
1.491MetGly: 1.491 ± 0.053
0.4MetHis: 0.4 ± 0.028
1.518MetIle: 1.518 ± 0.065
1.579MetLys: 1.579 ± 0.052
2.669MetLeu: 2.669 ± 0.076
0.659MetMet: 0.659 ± 0.035
1.199MetAsn: 1.199 ± 0.047
1.021MetPro: 1.021 ± 0.045
1.344MetGln: 1.344 ± 0.052
1.094MetArg: 1.094 ± 0.041
1.518MetSer: 1.518 ± 0.05
1.501MetThr: 1.501 ± 0.052
1.49MetVal: 1.49 ± 0.056
0.224MetTrp: 0.224 ± 0.021
0.541MetTyr: 0.541 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
4.158AsnAla: 4.158 ± 0.106
0.426AsnCys: 0.426 ± 0.029
2.342AsnAsp: 2.342 ± 0.098
2.738AsnGlu: 2.738 ± 0.071
1.802AsnPhe: 1.802 ± 0.053
3.589AsnGly: 3.589 ± 0.13
0.938AsnHis: 0.938 ± 0.038
3.386AsnIle: 3.386 ± 0.074
2.897AsnLys: 2.897 ± 0.108
4.696AsnLeu: 4.696 ± 0.091
1.105AsnMet: 1.105 ± 0.043
2.311AsnAsn: 2.311 ± 0.094
2.36AsnPro: 2.36 ± 0.061
2.334AsnGln: 2.334 ± 0.062
2.23AsnArg: 2.23 ± 0.063
2.763AsnSer: 2.763 ± 0.094
2.542AsnThr: 2.542 ± 0.081
3.332AsnVal: 3.332 ± 0.095
0.591AsnTrp: 0.591 ± 0.031
1.619AsnTyr: 1.619 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
2.826ProAla: 2.826 ± 0.07
0.304ProCys: 0.304 ± 0.023
1.857ProAsp: 1.857 ± 0.062
2.78ProGlu: 2.78 ± 0.068
1.751ProPhe: 1.751 ± 0.059
1.614ProGly: 1.614 ± 0.055
1.023ProHis: 1.023 ± 0.042
2.539ProIle: 2.539 ± 0.083
2.183ProLys: 2.183 ± 0.063
3.849ProLeu: 3.849 ± 0.099
0.928ProMet: 0.928 ± 0.043
1.973ProAsn: 1.973 ± 0.054
1.113ProPro: 1.113 ± 0.043
2.003ProGln: 2.003 ± 0.064
1.259ProArg: 1.259 ± 0.051
2.142ProSer: 2.142 ± 0.053
2.3ProThr: 2.3 ± 0.065
2.659ProVal: 2.659 ± 0.073
0.355ProTrp: 0.355 ± 0.027
1.344ProTyr: 1.344 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
4.535GlnAla: 4.535 ± 0.085
0.408GlnCys: 0.408 ± 0.03
2.355GlnAsp: 2.355 ± 0.069
2.662GlnGlu: 2.662 ± 0.076
2.024GlnPhe: 2.024 ± 0.063
3.327GlnGly: 3.327 ± 0.086
1.132GlnHis: 1.132 ± 0.048
3.171GlnIle: 3.171 ± 0.073
2.823GlnLys: 2.823 ± 0.073
5.392GlnLeu: 5.392 ± 0.116
0.971GlnMet: 0.971 ± 0.046
2.186GlnAsn: 2.186 ± 0.063
1.742GlnPro: 1.742 ± 0.061
3.69GlnGln: 3.69 ± 0.115
2.552GlnArg: 2.552 ± 0.084
2.577GlnSer: 2.577 ± 0.076
2.325GlnThr: 2.325 ± 0.068
3.007GlnVal: 3.007 ± 0.08
0.676GlnTrp: 0.676 ± 0.03
1.594GlnTyr: 1.594 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
3.315ArgAla: 3.315 ± 0.08
0.456ArgCys: 0.456 ± 0.03
2.172ArgAsp: 2.172 ± 0.067
2.977ArgGlu: 2.977 ± 0.094
2.196ArgPhe: 2.196 ± 0.067
2.732ArgGly: 2.732 ± 0.065
1.196ArgHis: 1.196 ± 0.043
3.093ArgIle: 3.093 ± 0.08
2.538ArgLys: 2.538 ± 0.078
5.134ArgLeu: 5.134 ± 0.112
1.045ArgMet: 1.045 ± 0.048
1.951ArgAsn: 1.951 ± 0.065
1.578ArgPro: 1.578 ± 0.055
2.587ArgGln: 2.587 ± 0.073
2.434ArgArg: 2.434 ± 0.084
2.303ArgSer: 2.303 ± 0.074
2.219ArgThr: 2.219 ± 0.059
2.884ArgVal: 2.884 ± 0.071
0.55ArgTrp: 0.55 ± 0.031
1.884ArgTyr: 1.884 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
5.458SerAla: 5.458 ± 0.103
0.549SerCys: 0.549 ± 0.03
3.091SerAsp: 3.091 ± 0.091
3.329SerGlu: 3.329 ± 0.074
2.571SerPhe: 2.571 ± 0.062
4.569SerGly: 4.569 ± 0.11
1.352SerHis: 1.352 ± 0.05
3.773SerIle: 3.773 ± 0.09
3.029SerLys: 3.029 ± 0.089
5.886SerLeu: 5.886 ± 0.125
1.31SerMet: 1.31 ± 0.048
2.497SerAsn: 2.497 ± 0.087
2.117SerPro: 2.117 ± 0.063
2.705SerGln: 2.705 ± 0.071
2.535SerArg: 2.535 ± 0.063
3.498SerSer: 3.498 ± 0.089
3.064SerThr: 3.064 ± 0.092
4.286SerVal: 4.286 ± 0.092
0.676SerTrp: 0.676 ± 0.031
1.844SerTyr: 1.844 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.826ThrAla: 4.826 ± 0.121
0.443ThrCys: 0.443 ± 0.028
2.749ThrAsp: 2.749 ± 0.08
3.133ThrGlu: 3.133 ± 0.073
2.208ThrPhe: 2.208 ± 0.059
4.046ThrGly: 4.046 ± 0.14
1.174ThrHis: 1.174 ± 0.041
3.401ThrIle: 3.401 ± 0.079
2.807ThrLys: 2.807 ± 0.1
6.191ThrLeu: 6.191 ± 0.117
1.006ThrMet: 1.006 ± 0.04
2.232ThrAsn: 2.232 ± 0.083
2.615ThrPro: 2.615 ± 0.072
2.527ThrGln: 2.527 ± 0.072
2.043ThrArg: 2.043 ± 0.064
2.75ThrSer: 2.75 ± 0.075
2.908ThrThr: 2.908 ± 0.099
3.849ThrVal: 3.849 ± 0.11
0.561ThrTrp: 0.561 ± 0.033
1.499ThrTyr: 1.499 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
6.28ValAla: 6.28 ± 0.122
0.682ValCys: 0.682 ± 0.033
3.591ValAsp: 3.591 ± 0.075
4.429ValGlu: 4.429 ± 0.087
2.582ValPhe: 2.582 ± 0.081
4.787ValGly: 4.787 ± 0.107
1.166ValHis: 1.166 ± 0.044
5.036ValIle: 5.036 ± 0.08
4.317ValLys: 4.317 ± 0.092
6.538ValLeu: 6.538 ± 0.121
1.664ValMet: 1.664 ± 0.057
3.267ValAsn: 3.267 ± 0.081
2.407ValPro: 2.407 ± 0.067
2.569ValGln: 2.569 ± 0.075
3.036ValArg: 3.036 ± 0.084
4.15ValSer: 4.15 ± 0.081
3.512ValThr: 3.512 ± 0.086
4.963ValVal: 4.963 ± 0.101
0.638ValTrp: 0.638 ± 0.033
1.877ValTyr: 1.877 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.799TrpAla: 0.799 ± 0.035
0.15TrpCys: 0.15 ± 0.016
0.517TrpAsp: 0.517 ± 0.026
0.577TrpGlu: 0.577 ± 0.033
0.556TrpPhe: 0.556 ± 0.032
0.684TrpGly: 0.684 ± 0.034
0.265TrpHis: 0.265 ± 0.02
0.739TrpIle: 0.739 ± 0.034
0.638TrpLys: 0.638 ± 0.034
1.598TrpLeu: 1.598 ± 0.058
0.249TrpMet: 0.249 ± 0.019
0.501TrpAsn: 0.501 ± 0.029
0.158TrpPro: 0.158 ± 0.015
1.067TrpGln: 1.067 ± 0.043
0.659TrpArg: 0.659 ± 0.036
0.571TrpSer: 0.571 ± 0.034
0.462TrpThr: 0.462 ± 0.029
0.764TrpVal: 0.764 ± 0.035
0.173TrpTrp: 0.173 ± 0.019
0.361TrpTyr: 0.361 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.577TyrAla: 2.577 ± 0.068
0.444TyrCys: 0.444 ± 0.033
1.69TyrAsp: 1.69 ± 0.052
1.526TyrGlu: 1.526 ± 0.059
1.704TyrPhe: 1.704 ± 0.058
2.219TyrGly: 2.219 ± 0.063
0.846TyrHis: 0.846 ± 0.038
1.794TyrIle: 1.794 ± 0.063
1.532TyrLys: 1.532 ± 0.058
3.378TyrLeu: 3.378 ± 0.076
0.597TyrMet: 0.597 ± 0.032
1.305TyrAsn: 1.305 ± 0.048
1.425TyrPro: 1.425 ± 0.049
1.983TyrGln: 1.983 ± 0.059
1.822TyrArg: 1.822 ± 0.058
1.906TyrSer: 1.906 ± 0.057
1.483TyrThr: 1.483 ± 0.045
2.049TyrVal: 2.049 ± 0.051
0.462TyrTrp: 0.462 ± 0.03
1.129TyrTyr: 1.129 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2028 proteins (634438 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski