Streptococcus satellite phage Javan574

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW09|A0A4D5ZW09_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan574 OX=2558755 GN=JavanS574_0006 PE=4 SV=1
MM1 pKa = 7.56NEE3 pKa = 4.1LDD5 pKa = 4.25LTPGQYY11 pKa = 10.71LAFILITLVLLAYY24 pKa = 9.62EE25 pKa = 5.02LYY27 pKa = 10.57RR28 pKa = 11.84FYY30 pKa = 11.27NDD32 pKa = 3.26SEE34 pKa = 4.58IIEE37 pKa = 5.18LPDD40 pKa = 3.64KK41 pKa = 10.45PQEE44 pKa = 4.24EE45 pKa = 4.67PASQQLNPNYY55 pKa = 10.29GAYY58 pKa = 9.13IWLAGRR64 pKa = 11.84RR65 pKa = 11.84YY66 pKa = 9.82NN67 pKa = 4.44

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZQY4|A0A4D5ZQY4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan574 OX=2558755 GN=JavanS574_0011 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.33ITEE5 pKa = 4.19VKK7 pKa = 9.77KK8 pKa = 10.92KK9 pKa = 10.69NGATVYY15 pKa = 10.17RR16 pKa = 11.84ASVYY20 pKa = 10.76LGVDD24 pKa = 2.9QVTGKK29 pKa = 10.2KK30 pKa = 10.32VKK32 pKa = 9.81TKK34 pKa = 9.67VTGRR38 pKa = 11.84TQKK41 pKa = 10.06EE42 pKa = 4.34VKK44 pKa = 10.06QKK46 pKa = 11.0AKK48 pKa = 9.6QEE50 pKa = 4.44KK51 pKa = 8.56ITFQQDD57 pKa = 2.5GFTRR61 pKa = 11.84FQATSIASYY70 pKa = 11.04QEE72 pKa = 4.96LSSLWWEE79 pKa = 4.51SYY81 pKa = 9.66KK82 pKa = 9.92HH83 pKa = 4.54TVKK86 pKa = 10.73PNTQDD91 pKa = 3.23NVKK94 pKa = 10.45KK95 pKa = 10.83LLDD98 pKa = 3.61NHH100 pKa = 6.25VLPLFGVYY108 pKa = 10.41KK109 pKa = 10.33LDD111 pKa = 3.96KK112 pKa = 9.96LTTPLIQSIVNKK124 pKa = 10.33LADD127 pKa = 3.81KK128 pKa = 9.77TNKK131 pKa = 10.04GEE133 pKa = 4.16PGAYY137 pKa = 9.37LHH139 pKa = 6.54YY140 pKa = 10.99DD141 pKa = 3.97KK142 pKa = 11.05IHH144 pKa = 6.58ALNKK148 pKa = 10.18RR149 pKa = 11.84ILQYY153 pKa = 10.89GVTMQAISSNPARR166 pKa = 11.84DD167 pKa = 3.32VVLPRR172 pKa = 11.84NTQKK176 pKa = 11.2AKK178 pKa = 9.91RR179 pKa = 11.84KK180 pKa = 8.83KK181 pKa = 9.6VKK183 pKa = 10.37HH184 pKa = 6.08FEE186 pKa = 4.05NQDD189 pKa = 3.16LKK191 pKa = 11.47KK192 pKa = 10.72FLDD195 pKa = 3.76YY196 pKa = 11.37LGGLDD201 pKa = 3.33QAKK204 pKa = 9.96YY205 pKa = 9.87RR206 pKa = 11.84NLYY209 pKa = 9.15EE210 pKa = 3.83ATLYY214 pKa = 10.98KK215 pKa = 10.43FLLATGCRR223 pKa = 11.84INEE226 pKa = 4.0ALALSWSDD234 pKa = 2.93IDD236 pKa = 5.64LEE238 pKa = 4.2NATISITKK246 pKa = 8.36TLNHH250 pKa = 7.1LGQINSPKK258 pKa = 10.48SKK260 pKa = 10.64ASYY263 pKa = 10.37RR264 pKa = 11.84DD265 pKa = 2.9IDD267 pKa = 3.66IDD269 pKa = 3.88QEE271 pKa = 4.56TITMLKK277 pKa = 10.13AYY279 pKa = 7.54QLRR282 pKa = 11.84QIQEE286 pKa = 3.57AWKK289 pKa = 10.1LGQTEE294 pKa = 4.3TVVFSDD300 pKa = 6.17FIHH303 pKa = 7.81DD304 pKa = 3.85YY305 pKa = 11.0PNNKK309 pKa = 8.11TLATRR314 pKa = 11.84LRR316 pKa = 11.84THH318 pKa = 7.06FKK320 pKa = 10.33RR321 pKa = 11.84AGVSNIGFHH330 pKa = 6.37GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.58ASLLLNSGIPYY347 pKa = 10.0KK348 pKa = 10.34EE349 pKa = 3.8LQHH352 pKa = 6.73RR353 pKa = 11.84LGHH356 pKa = 5.68STLSMTMDD364 pKa = 3.58IYY366 pKa = 11.68SHH368 pKa = 6.98LSKK371 pKa = 11.0EE372 pKa = 4.25NAKK375 pKa = 10.37KK376 pKa = 10.37AVSFYY381 pKa = 9.42EE382 pKa = 4.24TALKK386 pKa = 10.67ALL388 pKa = 4.15

Molecular weight: 44.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2316

57

500

178.2

20.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.304 ± 0.436

0.389 ± 0.107

5.959 ± 0.675

8.204 ± 0.726

4.059 ± 0.398

4.922 ± 0.398

1.9 ± 0.395

6.174 ± 0.385

9.326 ± 0.804

10.579 ± 0.716

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.116 ± 0.23

5.311 ± 0.503

3.8 ± 0.609

4.188 ± 0.375

4.663 ± 0.518

4.663 ± 0.456

6.52 ± 0.377

5.397 ± 0.32

0.95 ± 0.188

4.577 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski