Amino acid dipepetide frequency for Propionibacterium phage PAS50

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.977AlaAla: 9.977 ± 1.565
1.134AlaCys: 1.134 ± 0.387
6.349AlaAsp: 6.349 ± 0.982
5.215AlaGlu: 5.215 ± 0.773
2.721AlaPhe: 2.721 ± 0.533
9.637AlaGly: 9.637 ± 1.373
2.041AlaHis: 2.041 ± 0.541
4.082AlaIle: 4.082 ± 0.74
4.082AlaLys: 4.082 ± 0.647
7.029AlaLeu: 7.029 ± 0.923
3.288AlaMet: 3.288 ± 1.133
2.154AlaAsn: 2.154 ± 0.417
2.154AlaPro: 2.154 ± 0.428
4.082AlaGln: 4.082 ± 0.64
5.782AlaArg: 5.782 ± 0.98
6.689AlaSer: 6.689 ± 0.82
6.009AlaThr: 6.009 ± 0.734
8.844AlaVal: 8.844 ± 1.145
1.361AlaTrp: 1.361 ± 0.295
2.834AlaTyr: 2.834 ± 0.509
0.0AlaXaa: 0.0 ± 0.0
Cys
1.361CysAla: 1.361 ± 0.432
0.227CysCys: 0.227 ± 0.168
1.361CysAsp: 1.361 ± 0.457
1.134CysGlu: 1.134 ± 0.324
0.0CysPhe: 0.0 ± 0.0
1.474CysGly: 1.474 ± 0.42
0.567CysHis: 0.567 ± 0.278
0.227CysIle: 0.227 ± 0.165
0.68CysLys: 0.68 ± 0.375
0.454CysLeu: 0.454 ± 0.222
0.0CysMet: 0.0 ± 0.0
0.227CysAsn: 0.227 ± 0.143
1.134CysPro: 1.134 ± 0.394
0.227CysGln: 0.227 ± 0.138
1.474CysArg: 1.474 ± 0.435
0.68CysSer: 0.68 ± 0.324
1.134CysThr: 1.134 ± 0.301
0.567CysVal: 0.567 ± 0.238
0.567CysTrp: 0.567 ± 0.296
0.227CysTyr: 0.227 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
4.649AspAla: 4.649 ± 0.638
0.794AspCys: 0.794 ± 0.255
5.215AspAsp: 5.215 ± 0.789
3.741AspGlu: 3.741 ± 0.563
1.701AspPhe: 1.701 ± 0.459
6.463AspGly: 6.463 ± 1.942
2.154AspHis: 2.154 ± 0.512
2.381AspIle: 2.381 ± 0.591
2.608AspLys: 2.608 ± 0.622
4.535AspLeu: 4.535 ± 0.818
1.361AspMet: 1.361 ± 0.355
2.608AspAsn: 2.608 ± 0.635
3.628AspPro: 3.628 ± 0.835
1.587AspGln: 1.587 ± 0.36
4.082AspArg: 4.082 ± 0.676
3.515AspSer: 3.515 ± 0.561
4.875AspThr: 4.875 ± 0.677
4.989AspVal: 4.989 ± 0.747
1.361AspTrp: 1.361 ± 0.375
2.494AspTyr: 2.494 ± 0.619
0.0AspXaa: 0.0 ± 0.0
Glu
4.989GluAla: 4.989 ± 0.81
0.454GluCys: 0.454 ± 0.202
1.701GluAsp: 1.701 ± 0.512
1.814GluGlu: 1.814 ± 0.517
1.587GluPhe: 1.587 ± 0.306
3.401GluGly: 3.401 ± 0.581
0.907GluHis: 0.907 ± 0.288
1.814GluIle: 1.814 ± 0.388
1.474GluLys: 1.474 ± 0.361
3.515GluLeu: 3.515 ± 0.759
1.134GluMet: 1.134 ± 0.327
1.474GluAsn: 1.474 ± 0.412
1.361GluPro: 1.361 ± 0.479
2.834GluGln: 2.834 ± 0.484
3.175GluArg: 3.175 ± 0.833
4.308GluSer: 4.308 ± 0.969
3.175GluThr: 3.175 ± 0.749
3.401GluVal: 3.401 ± 0.679
2.154GluTrp: 2.154 ± 0.568
1.701GluTyr: 1.701 ± 0.459
0.0GluXaa: 0.0 ± 0.0
Phe
2.721PheAla: 2.721 ± 0.857
0.34PheCys: 0.34 ± 0.185
2.041PheAsp: 2.041 ± 0.521
1.361PheGlu: 1.361 ± 0.349
1.247PhePhe: 1.247 ± 0.318
2.268PheGly: 2.268 ± 0.53
1.02PheHis: 1.02 ± 0.328
1.134PheIle: 1.134 ± 0.297
1.474PheLys: 1.474 ± 0.448
1.814PheLeu: 1.814 ± 0.439
0.907PheMet: 0.907 ± 0.301
0.907PheAsn: 0.907 ± 0.38
1.361PhePro: 1.361 ± 0.369
1.02PheGln: 1.02 ± 0.249
1.814PheArg: 1.814 ± 0.49
2.268PheSer: 2.268 ± 0.5
2.041PheThr: 2.041 ± 0.455
1.701PheVal: 1.701 ± 0.527
0.567PheTrp: 0.567 ± 0.248
0.113PheTyr: 0.113 ± 0.1
0.0PheXaa: 0.0 ± 0.0
Gly
6.463GlyAla: 6.463 ± 1.378
0.907GlyCys: 0.907 ± 0.386
5.782GlyAsp: 5.782 ± 0.906
4.762GlyGlu: 4.762 ± 0.797
2.834GlyPhe: 2.834 ± 0.755
6.803GlyGly: 6.803 ± 1.021
1.587GlyHis: 1.587 ± 0.432
2.721GlyIle: 2.721 ± 0.781
4.875GlyLys: 4.875 ± 0.669
7.823GlyLeu: 7.823 ± 1.264
2.154GlyMet: 2.154 ± 0.569
2.268GlyAsn: 2.268 ± 0.444
3.628GlyPro: 3.628 ± 1.044
3.288GlyGln: 3.288 ± 0.511
5.102GlyArg: 5.102 ± 1.018
6.689GlySer: 6.689 ± 1.161
4.422GlyThr: 4.422 ± 0.651
9.524GlyVal: 9.524 ± 1.542
1.927GlyTrp: 1.927 ± 0.437
2.948GlyTyr: 2.948 ± 0.519
0.0GlyXaa: 0.0 ± 0.0
His
1.701HisAla: 1.701 ± 0.528
1.02HisCys: 1.02 ± 0.3
1.474HisAsp: 1.474 ± 0.497
0.567HisGlu: 0.567 ± 0.252
0.567HisPhe: 0.567 ± 0.332
1.927HisGly: 1.927 ± 0.366
1.474HisHis: 1.474 ± 0.421
1.474HisIle: 1.474 ± 0.379
1.247HisLys: 1.247 ± 0.42
2.494HisLeu: 2.494 ± 0.626
0.454HisMet: 0.454 ± 0.198
1.134HisAsn: 1.134 ± 0.359
1.587HisPro: 1.587 ± 0.559
1.474HisGln: 1.474 ± 0.49
1.587HisArg: 1.587 ± 0.373
1.814HisSer: 1.814 ± 0.491
2.494HisThr: 2.494 ± 0.709
1.587HisVal: 1.587 ± 0.532
0.454HisTrp: 0.454 ± 0.243
0.907HisTyr: 0.907 ± 0.407
0.0HisXaa: 0.0 ± 0.0
Ile
3.968IleAla: 3.968 ± 0.651
0.794IleCys: 0.794 ± 0.297
4.422IleAsp: 4.422 ± 0.625
3.061IleGlu: 3.061 ± 0.646
1.134IlePhe: 1.134 ± 0.305
2.381IleGly: 2.381 ± 0.751
1.134IleHis: 1.134 ± 0.344
3.741IleIle: 3.741 ± 0.682
2.154IleLys: 2.154 ± 0.437
2.608IleLeu: 2.608 ± 0.438
1.134IleMet: 1.134 ± 0.394
1.927IleAsn: 1.927 ± 0.559
2.494IlePro: 2.494 ± 0.448
2.154IleGln: 2.154 ± 0.406
2.494IleArg: 2.494 ± 0.479
2.494IleSer: 2.494 ± 0.53
3.628IleThr: 3.628 ± 0.568
3.401IleVal: 3.401 ± 0.556
0.34IleTrp: 0.34 ± 0.193
1.361IleTyr: 1.361 ± 0.418
0.0IleXaa: 0.0 ± 0.0
Lys
4.875LysAla: 4.875 ± 0.874
0.34LysCys: 0.34 ± 0.152
3.175LysAsp: 3.175 ± 0.679
0.794LysGlu: 0.794 ± 0.234
0.794LysPhe: 0.794 ± 0.234
4.308LysGly: 4.308 ± 0.568
0.907LysHis: 0.907 ± 0.32
1.701LysIle: 1.701 ± 0.516
1.474LysLys: 1.474 ± 0.372
3.288LysLeu: 3.288 ± 0.531
0.68LysMet: 0.68 ± 0.246
1.814LysAsn: 1.814 ± 0.427
3.175LysPro: 3.175 ± 0.7
2.608LysGln: 2.608 ± 0.573
3.175LysArg: 3.175 ± 0.659
2.041LysSer: 2.041 ± 0.738
3.288LysThr: 3.288 ± 0.71
1.474LysVal: 1.474 ± 0.414
0.68LysTrp: 0.68 ± 0.232
1.247LysTyr: 1.247 ± 0.372
0.0LysXaa: 0.0 ± 0.0
Leu
9.07LeuAla: 9.07 ± 1.012
1.474LeuCys: 1.474 ± 0.431
5.102LeuAsp: 5.102 ± 0.959
2.948LeuGlu: 2.948 ± 0.636
1.701LeuPhe: 1.701 ± 0.456
6.349LeuGly: 6.349 ± 1.156
2.608LeuHis: 2.608 ± 0.503
2.948LeuIle: 2.948 ± 0.674
4.422LeuLys: 4.422 ± 0.639
4.535LeuLeu: 4.535 ± 0.704
1.474LeuMet: 1.474 ± 0.398
2.721LeuAsn: 2.721 ± 0.458
4.649LeuPro: 4.649 ± 0.701
2.948LeuGln: 2.948 ± 0.535
3.061LeuArg: 3.061 ± 0.487
6.122LeuSer: 6.122 ± 0.872
4.875LeuThr: 4.875 ± 0.91
3.968LeuVal: 3.968 ± 0.716
0.794LeuTrp: 0.794 ± 0.323
1.701LeuTyr: 1.701 ± 0.504
0.0LeuXaa: 0.0 ± 0.0
Met
3.175MetAla: 3.175 ± 0.525
0.68MetCys: 0.68 ± 0.247
1.02MetAsp: 1.02 ± 0.356
0.68MetGlu: 0.68 ± 0.337
1.02MetPhe: 1.02 ± 0.348
1.701MetGly: 1.701 ± 0.563
0.794MetHis: 0.794 ± 0.28
1.701MetIle: 1.701 ± 0.379
0.794MetLys: 0.794 ± 0.331
2.381MetLeu: 2.381 ± 0.454
0.454MetMet: 0.454 ± 0.183
1.02MetAsn: 1.02 ± 0.381
1.474MetPro: 1.474 ± 0.608
1.02MetGln: 1.02 ± 0.307
1.247MetArg: 1.247 ± 0.328
1.814MetSer: 1.814 ± 0.406
1.247MetThr: 1.247 ± 0.358
1.814MetVal: 1.814 ± 0.45
0.567MetTrp: 0.567 ± 0.247
1.02MetTyr: 1.02 ± 0.407
0.0MetXaa: 0.0 ± 0.0
Asn
2.721AsnAla: 2.721 ± 0.932
0.113AsnCys: 0.113 ± 0.1
1.474AsnAsp: 1.474 ± 0.403
1.134AsnGlu: 1.134 ± 0.308
0.567AsnPhe: 0.567 ± 0.218
5.215AsnGly: 5.215 ± 0.891
1.474AsnHis: 1.474 ± 0.449
1.814AsnIle: 1.814 ± 0.498
1.587AsnLys: 1.587 ± 0.521
2.041AsnLeu: 2.041 ± 0.482
1.247AsnMet: 1.247 ± 0.294
2.154AsnAsn: 2.154 ± 0.839
2.834AsnPro: 2.834 ± 0.739
1.474AsnGln: 1.474 ± 0.41
2.041AsnArg: 2.041 ± 0.469
1.927AsnSer: 1.927 ± 0.594
2.381AsnThr: 2.381 ± 0.546
2.834AsnVal: 2.834 ± 0.521
0.567AsnTrp: 0.567 ± 0.235
0.907AsnTyr: 0.907 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
5.102ProAla: 5.102 ± 0.987
0.34ProCys: 0.34 ± 0.202
4.195ProAsp: 4.195 ± 0.85
2.721ProGlu: 2.721 ± 0.638
1.134ProPhe: 1.134 ± 0.338
5.329ProGly: 5.329 ± 0.99
1.814ProHis: 1.814 ± 0.494
1.701ProIle: 1.701 ± 0.396
1.701ProLys: 1.701 ± 0.446
3.288ProLeu: 3.288 ± 0.613
0.907ProMet: 0.907 ± 0.334
2.154ProAsn: 2.154 ± 0.507
3.061ProPro: 3.061 ± 0.718
1.701ProGln: 1.701 ± 0.5
2.041ProArg: 2.041 ± 0.466
3.515ProSer: 3.515 ± 0.646
2.608ProThr: 2.608 ± 0.631
5.329ProVal: 5.329 ± 1.172
1.587ProTrp: 1.587 ± 0.476
0.907ProTyr: 0.907 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
4.195GlnAla: 4.195 ± 0.818
0.454GlnCys: 0.454 ± 0.278
1.701GlnAsp: 1.701 ± 0.348
1.361GlnGlu: 1.361 ± 0.389
0.794GlnPhe: 0.794 ± 0.262
1.814GlnGly: 1.814 ± 0.603
1.474GlnHis: 1.474 ± 0.534
2.834GlnIle: 2.834 ± 0.605
1.02GlnLys: 1.02 ± 0.332
4.082GlnLeu: 4.082 ± 0.667
1.134GlnMet: 1.134 ± 0.374
1.247GlnAsn: 1.247 ± 0.311
3.175GlnPro: 3.175 ± 0.767
3.175GlnGln: 3.175 ± 0.655
2.834GlnArg: 2.834 ± 0.521
1.701GlnSer: 1.701 ± 0.437
3.061GlnThr: 3.061 ± 0.69
2.834GlnVal: 2.834 ± 0.557
1.02GlnTrp: 1.02 ± 0.295
1.361GlnTyr: 1.361 ± 0.393
0.0GlnXaa: 0.0 ± 0.0
Arg
5.896ArgAla: 5.896 ± 0.946
0.567ArgCys: 0.567 ± 0.245
3.628ArgAsp: 3.628 ± 0.752
2.721ArgGlu: 2.721 ± 0.595
2.268ArgPhe: 2.268 ± 0.527
3.968ArgGly: 3.968 ± 0.672
1.474ArgHis: 1.474 ± 0.341
3.401ArgIle: 3.401 ± 0.665
3.061ArgLys: 3.061 ± 0.687
6.689ArgLeu: 6.689 ± 0.692
1.814ArgMet: 1.814 ± 0.447
2.608ArgAsn: 2.608 ± 0.707
1.701ArgPro: 1.701 ± 0.443
2.381ArgGln: 2.381 ± 0.615
5.102ArgArg: 5.102 ± 0.891
4.535ArgSer: 4.535 ± 0.713
2.721ArgThr: 2.721 ± 0.559
4.875ArgVal: 4.875 ± 0.844
0.907ArgTrp: 0.907 ± 0.319
1.927ArgTyr: 1.927 ± 0.459
0.0ArgXaa: 0.0 ± 0.0
Ser
6.916SerAla: 6.916 ± 0.871
0.567SerCys: 0.567 ± 0.326
3.855SerAsp: 3.855 ± 0.854
2.834SerGlu: 2.834 ± 0.543
3.288SerPhe: 3.288 ± 0.544
8.39SerGly: 8.39 ± 1.231
1.701SerHis: 1.701 ± 0.466
3.175SerIle: 3.175 ± 0.493
2.041SerLys: 2.041 ± 0.585
3.968SerLeu: 3.968 ± 0.578
2.041SerMet: 2.041 ± 0.337
2.494SerAsn: 2.494 ± 0.553
3.175SerPro: 3.175 ± 0.751
2.041SerGln: 2.041 ± 0.421
4.308SerArg: 4.308 ± 0.713
4.875SerSer: 4.875 ± 0.9
3.855SerThr: 3.855 ± 1.005
6.349SerVal: 6.349 ± 1.139
1.814SerTrp: 1.814 ± 0.382
0.68SerTyr: 0.68 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
6.463ThrAla: 6.463 ± 0.885
1.247ThrCys: 1.247 ± 0.399
3.855ThrAsp: 3.855 ± 0.535
2.154ThrGlu: 2.154 ± 0.503
1.814ThrPhe: 1.814 ± 0.411
5.215ThrGly: 5.215 ± 0.63
1.361ThrHis: 1.361 ± 0.47
4.649ThrIle: 4.649 ± 0.693
1.927ThrLys: 1.927 ± 0.435
4.082ThrLeu: 4.082 ± 0.896
1.361ThrMet: 1.361 ± 0.352
1.927ThrAsn: 1.927 ± 0.478
4.195ThrPro: 4.195 ± 0.769
2.721ThrGln: 2.721 ± 0.553
3.628ThrArg: 3.628 ± 0.554
4.535ThrSer: 4.535 ± 0.68
4.535ThrThr: 4.535 ± 1.06
5.782ThrVal: 5.782 ± 0.652
1.134ThrTrp: 1.134 ± 0.325
1.701ThrTyr: 1.701 ± 0.438
0.0ThrXaa: 0.0 ± 0.0
Val
7.483ValAla: 7.483 ± 1.381
1.247ValCys: 1.247 ± 0.379
5.442ValAsp: 5.442 ± 0.892
4.762ValGlu: 4.762 ± 0.755
1.927ValPhe: 1.927 ± 0.589
6.009ValGly: 6.009 ± 1.051
1.814ValHis: 1.814 ± 0.411
3.061ValIle: 3.061 ± 1.155
3.628ValLys: 3.628 ± 0.75
6.236ValLeu: 6.236 ± 0.74
2.721ValMet: 2.721 ± 0.665
3.401ValAsn: 3.401 ± 0.72
3.741ValPro: 3.741 ± 0.625
2.494ValGln: 2.494 ± 0.588
4.535ValArg: 4.535 ± 0.545
6.236ValSer: 6.236 ± 0.87
4.422ValThr: 4.422 ± 0.621
6.803ValVal: 6.803 ± 1.484
1.474ValTrp: 1.474 ± 0.509
1.361ValTyr: 1.361 ± 0.455
0.0ValXaa: 0.0 ± 0.0
Trp
1.814TrpAla: 1.814 ± 0.46
0.454TrpCys: 0.454 ± 0.256
0.794TrpAsp: 0.794 ± 0.264
1.134TrpGlu: 1.134 ± 0.326
0.454TrpPhe: 0.454 ± 0.292
1.361TrpGly: 1.361 ± 0.48
0.567TrpHis: 0.567 ± 0.295
1.02TrpIle: 1.02 ± 0.289
0.794TrpLys: 0.794 ± 0.341
1.247TrpLeu: 1.247 ± 0.431
0.794TrpMet: 0.794 ± 0.291
1.134TrpAsn: 1.134 ± 0.334
0.68TrpPro: 0.68 ± 0.221
1.02TrpGln: 1.02 ± 0.343
2.268TrpArg: 2.268 ± 0.567
1.247TrpSer: 1.247 ± 0.415
1.361TrpThr: 1.361 ± 0.302
0.794TrpVal: 0.794 ± 0.335
0.454TrpTrp: 0.454 ± 0.198
0.794TrpTyr: 0.794 ± 0.319
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.927TyrAla: 1.927 ± 0.407
0.567TyrCys: 0.567 ± 0.239
2.154TyrAsp: 2.154 ± 0.533
1.474TyrGlu: 1.474 ± 0.349
0.567TyrPhe: 0.567 ± 0.225
2.494TyrGly: 2.494 ± 0.422
0.567TyrHis: 0.567 ± 0.232
1.247TyrIle: 1.247 ± 0.368
0.794TyrLys: 0.794 ± 0.307
1.361TyrLeu: 1.361 ± 0.357
0.454TyrMet: 0.454 ± 0.222
1.134TyrAsn: 1.134 ± 0.399
1.927TyrPro: 1.927 ± 0.519
1.02TyrGln: 1.02 ± 0.395
2.494TyrArg: 2.494 ± 0.51
1.361TyrSer: 1.361 ± 0.389
2.154TyrThr: 2.154 ± 0.408
1.927TyrVal: 1.927 ± 0.455
0.567TyrTrp: 0.567 ± 0.235
0.907TyrTyr: 0.907 ± 0.301
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (8821 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski