Acholeplasma palmae (strain ATCC 49389 / J233)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma; Acholeplasma palmae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1433 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4KJS2|U4KJS2_ACHPJ Putative septation protein SpoVG OS=Acholeplasma palmae (strain ATCC 49389 / J233) OX=1318466 GN=spoVG PE=3 SV=1
MM1 pKa = 7.81NEE3 pKa = 3.73EE4 pKa = 3.82QQAMEE9 pKa = 4.26EE10 pKa = 4.16QVKK13 pKa = 10.42VLIDD17 pKa = 3.31KK18 pKa = 8.97VRR20 pKa = 11.84PYY22 pKa = 10.57LQRR25 pKa = 11.84DD26 pKa = 3.44GGDD29 pKa = 3.12IEE31 pKa = 4.74IMNIEE36 pKa = 4.35DD37 pKa = 4.24GIVYY41 pKa = 9.94VQMLGACDD49 pKa = 3.37GCMAIDD55 pKa = 3.39ITLKK59 pKa = 10.68QGIEE63 pKa = 3.97TMLLEE68 pKa = 4.29NVPGVIAVVTVV79 pKa = 3.41

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4KN43|U4KN43_ACHPJ Predicted transcriptional regulator OS=Acholeplasma palmae (strain ATCC 49389 / J233) OX=1318466 GN=BN85400080 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.38RR10 pKa = 11.84KK11 pKa = 9.49RR12 pKa = 11.84QKK14 pKa = 8.39THH16 pKa = 4.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATASGRR28 pKa = 11.84KK29 pKa = 8.88VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84AQLTVV44 pKa = 2.91

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1433

0

1433

465257

37

5300

324.7

37.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.305 ± 0.074

0.454 ± 0.018

5.529 ± 0.046

7.268 ± 0.073

4.579 ± 0.045

5.417 ± 0.07

1.696 ± 0.028

9.62 ± 0.075

9.327 ± 0.07

10.003 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.035

6.121 ± 0.063

2.666 ± 0.031

3.084 ± 0.035

3.126 ± 0.042

6.024 ± 0.048

5.744 ± 0.068

6.371 ± 0.056

0.659 ± 0.019

4.761 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski