Prevotellaceae bacterium KH2P17

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; unclassified Prevotellaceae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239RJZ0|A0A239RJZ0_9BACT Alpha-amylase OS=Prevotellaceae bacterium KH2P17 OX=1945886 GN=SAMN06298210_10875 PE=3 SV=1
MM1 pKa = 7.89DD2 pKa = 5.15LSEE5 pKa = 3.8ARR7 pKa = 11.84IYY9 pKa = 10.82VGTYY13 pKa = 9.66AKK15 pKa = 10.48YY16 pKa = 11.08NNGSLQGEE24 pKa = 4.35WVEE27 pKa = 5.59LSDD30 pKa = 6.78FYY32 pKa = 11.84DD33 pKa = 4.32LDD35 pKa = 4.61DD36 pKa = 4.27FLEE39 pKa = 4.66RR40 pKa = 11.84CAEE43 pKa = 3.7IHH45 pKa = 6.55EE46 pKa = 5.36DD47 pKa = 3.81EE48 pKa = 4.77DD49 pKa = 3.82HH50 pKa = 6.99PEE52 pKa = 3.77YY53 pKa = 10.56MFQSWEE59 pKa = 4.45AIPDD63 pKa = 3.76CLIDD67 pKa = 4.34EE68 pKa = 4.51GHH70 pKa = 7.11LDD72 pKa = 3.57EE73 pKa = 6.73AFFCLRR79 pKa = 11.84DD80 pKa = 3.62EE81 pKa = 4.4MDD83 pKa = 3.67RR84 pKa = 11.84LHH86 pKa = 7.04GKK88 pKa = 7.25EE89 pKa = 4.98QEE91 pKa = 4.54AFWVWAEE98 pKa = 4.12GEE100 pKa = 4.68SINLTQDD107 pKa = 2.9AYY109 pKa = 11.24DD110 pKa = 3.72LVKK113 pKa = 10.69SFQCAYY119 pKa = 8.39MGSYY123 pKa = 10.42ASKK126 pKa = 10.76EE127 pKa = 3.88DD128 pKa = 3.58FAEE131 pKa = 4.38DD132 pKa = 3.81FVKK135 pKa = 11.03LEE137 pKa = 3.92MEE139 pKa = 4.32LPEE142 pKa = 4.08FALIYY147 pKa = 10.51FDD149 pKa = 3.87FSKK152 pKa = 11.21YY153 pKa = 10.79ADD155 pKa = 4.53DD156 pKa = 5.77LFCTDD161 pKa = 3.87FWYY164 pKa = 10.44EE165 pKa = 3.67DD166 pKa = 3.99GYY168 pKa = 10.75VFRR171 pKa = 11.84NEE173 pKa = 3.68

Molecular weight: 20.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239RJD4|A0A239RJD4_9BACT Carboxylic ester hydrolase OS=Prevotellaceae bacterium KH2P17 OX=1945886 GN=SAMN06298210_101158 PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2620

0

2620

960076

27

3287

366.4

41.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.103 ± 0.048

1.227 ± 0.021

5.739 ± 0.034

5.91 ± 0.045

4.278 ± 0.026

7.192 ± 0.044

2.099 ± 0.021

5.96 ± 0.047

5.725 ± 0.039

9.045 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.022

4.795 ± 0.039

3.843 ± 0.025

3.854 ± 0.029

5.453 ± 0.036

5.912 ± 0.042

5.747 ± 0.031

6.651 ± 0.039

1.306 ± 0.02

4.356 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski