Testicularia cyperi

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Anthracoideaceae; Testicularia

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7036 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317XM11|A0A317XM11_9BASI Lon protease homolog OS=Testicularia cyperi OX=1882483 GN=BCV70DRAFT_200921 PE=3 SV=1
MM1 pKa = 7.36PVSYY5 pKa = 10.66YY6 pKa = 10.97DD7 pKa = 3.95EE8 pKa = 6.02IEE10 pKa = 4.57LEE12 pKa = 4.1DD13 pKa = 3.49MSYY16 pKa = 11.37DD17 pKa = 3.61EE18 pKa = 5.29EE19 pKa = 5.88KK20 pKa = 11.17DD21 pKa = 3.56LFHH24 pKa = 6.71YY25 pKa = 7.97PCPCGDD31 pKa = 3.17RR32 pKa = 11.84FEE34 pKa = 4.4ITRR37 pKa = 11.84SQLKK41 pKa = 10.11DD42 pKa = 3.25AEE44 pKa = 4.37DD45 pKa = 3.59VARR48 pKa = 11.84CPSCSLIIRR57 pKa = 11.84VVFDD61 pKa = 5.15PIDD64 pKa = 4.01FEE66 pKa = 7.6DD67 pKa = 5.06DD68 pKa = 3.71DD69 pKa = 4.38EE70 pKa = 4.9PPRR73 pKa = 11.84NTVDD77 pKa = 3.06TSSNSVVAAAAA88 pKa = 3.6

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317XSA5|A0A317XSA5_9BASI Uncharacterized protein OS=Testicularia cyperi OX=1882483 GN=BCV70DRAFT_223429 PE=4 SV=1
MM1 pKa = 7.23NAEE4 pKa = 4.83LLNSIQKK11 pKa = 10.67GKK13 pKa = 10.09GLKK16 pKa = 10.05KK17 pKa = 10.43AVTNDD22 pKa = 2.89RR23 pKa = 11.84SAPQIDD29 pKa = 4.16SKK31 pKa = 11.59SGGGGGGGGGSGIVKK46 pKa = 10.24SGASGGGGGGPAMGGPPGLGGLFAGGMPKK75 pKa = 10.58LKK77 pKa = 10.27PSGGAPAVNTSLGKK91 pKa = 9.82PPARR95 pKa = 11.84PGASAPPPPAPPSSSSAAAPPAPPPPPGRR124 pKa = 11.84PMPPRR129 pKa = 11.84GPPPPPSGAAPSLPSRR145 pKa = 11.84GVPPPPSRR153 pKa = 11.84PSPASSAAAPPPPPPPPPSAAAPAVPPTPRR183 pKa = 11.84RR184 pKa = 11.84PGGAAPPPPPPPPTAARR201 pKa = 11.84PAIGSSSAPPPPPPPPGRR219 pKa = 11.84PSPAFGAPPPPPSRR233 pKa = 11.84PSPAAASAVAPAAPPPPPPPSRR255 pKa = 11.84PSGAAPPPPPPPSAPAPGLPSRR277 pKa = 11.84GVPAPPPAPSSPSPAAAATPPRR299 pKa = 11.84RR300 pKa = 11.84SAVPPPPPSRR310 pKa = 11.84APASPSTAPPPPPPPPSSSSRR331 pKa = 11.84APPPSRR337 pKa = 11.84VPPPPVAAPPSRR349 pKa = 11.84VPPPPVAAPPISTPPSRR366 pKa = 11.84TASSTIIPPSRR377 pKa = 11.84PTNGPPSTPAAPTRR391 pKa = 11.84RR392 pKa = 11.84IPSPPPTSGSLSFTPSANFPPPRR415 pKa = 11.84AFNASAASQHH425 pKa = 6.55RR426 pKa = 11.84YY427 pKa = 8.88PSGASKK433 pKa = 10.8GSSFNFASLGG443 pKa = 3.66

Molecular weight: 41.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7036

0

7036

3782685

51

4397

537.6

58.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.471 ± 0.033

1.06 ± 0.01

5.821 ± 0.023

5.425 ± 0.024

3.204 ± 0.018

7.119 ± 0.029

2.493 ± 0.013

3.951 ± 0.018

4.272 ± 0.025

8.757 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.01

3.227 ± 0.013

6.158 ± 0.025

4.306 ± 0.024

6.55 ± 0.026

10.241 ± 0.05

5.947 ± 0.019

5.744 ± 0.024

1.142 ± 0.01

2.194 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski