Vibrio phage Vc1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Gutovirus; Vibrio virus Vc1

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2KUJ0|X2KUJ0_9CAUD DNA-directed DNA polymerase OS=Vibrio phage Vc1 OX=1480731 GN=PV_034 PE=3 SV=1
MM1 pKa = 7.09YY2 pKa = 9.95RR3 pKa = 11.84PLSFSDD9 pKa = 3.53WLEE12 pKa = 3.9TWEE15 pKa = 4.24VEE17 pKa = 4.88LMEE20 pKa = 4.95LPEE23 pKa = 5.16DD24 pKa = 4.22ADD26 pKa = 3.77LDD28 pKa = 4.19EE29 pKa = 5.24VYY31 pKa = 10.46KK32 pKa = 10.78QYY34 pKa = 11.36LEE36 pKa = 4.32SFEE39 pKa = 4.19EE40 pKa = 4.65AYY42 pKa = 9.93IDD44 pKa = 4.58ANTT47 pKa = 3.63

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2KT11|X2KT11_9CAUD Uncharacterized protein OS=Vibrio phage Vc1 OX=1480731 GN=PV_002 PE=4 SV=1
MM1 pKa = 7.5TKK3 pKa = 9.72TNVVVDD9 pKa = 4.3HH10 pKa = 6.39DD11 pKa = 4.44HH12 pKa = 6.44KK13 pKa = 11.31SGVIRR18 pKa = 11.84AALPRR23 pKa = 11.84AINGLEE29 pKa = 3.99GKK31 pKa = 9.86LVQLCIRR38 pKa = 11.84WGGCKK43 pKa = 10.15NKK45 pKa = 10.81GEE47 pKa = 4.51VIQMLRR53 pKa = 11.84GMADD57 pKa = 3.27YY58 pKa = 11.29LEE60 pKa = 4.35LHH62 pKa = 6.77RR63 pKa = 11.84VPQTEE68 pKa = 4.52WIHH71 pKa = 6.23PEE73 pKa = 3.76HH74 pKa = 6.46LTPAEE79 pKa = 4.13KK80 pKa = 9.9RR81 pKa = 11.84AKK83 pKa = 10.09ANKK86 pKa = 9.02KK87 pKa = 9.72ARR89 pKa = 11.84ARR91 pKa = 11.84YY92 pKa = 8.92AKK94 pKa = 10.3NKK96 pKa = 9.48EE97 pKa = 3.98KK98 pKa = 10.95

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

13111

47

1228

298.0

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.787 ± 0.424

1.083 ± 0.167

6.529 ± 0.276

7.078 ± 0.446

3.798 ± 0.205

7.452 ± 0.355

1.945 ± 0.187

5.217 ± 0.23

6.529 ± 0.432

7.986 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.127 ± 0.22

4.897 ± 0.284

3.356 ± 0.171

3.89 ± 0.298

5.049 ± 0.277

5.743 ± 0.267

6.033 ± 0.383

6.636 ± 0.302

1.373 ± 0.141

3.493 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski