Pseudacidovorax intermedius

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Pseudacidovorax

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A147HC35|A0A147HC35_9BURK Ribosomal RNA large subunit methyltransferase N OS=Pseudacidovorax intermedius OX=433924 GN=NS331_01930 PE=3 SV=1
MM1 pKa = 6.84TAVAEE6 pKa = 4.54TTAPAVQAMPEE17 pKa = 4.39PIVFTDD23 pKa = 3.63SAAAKK28 pKa = 10.02VADD31 pKa = 5.45LIAEE35 pKa = 4.31EE36 pKa = 4.82GNPDD40 pKa = 2.99LKK42 pKa = 11.13LRR44 pKa = 11.84VFVQGGGCSGFQYY57 pKa = 10.9GFTFDD62 pKa = 3.93EE63 pKa = 4.69VTNEE67 pKa = 4.88DD68 pKa = 4.12DD69 pKa = 3.37TTMTKK74 pKa = 10.66NGVSLLIDD82 pKa = 3.54AMSYY86 pKa = 10.32QYY88 pKa = 11.5LVGAEE93 pKa = 3.79IDD95 pKa = 3.91YY96 pKa = 11.36KK97 pKa = 11.19EE98 pKa = 4.67DD99 pKa = 3.23LQGAQFVIKK108 pKa = 10.4NPNATTTCGCGSSFSTT124 pKa = 4.55

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A147GS64|A0A147GS64_9BURK Integral membrane protein OS=Pseudacidovorax intermedius OX=433924 GN=NS331_14290 PE=4 SV=1
MM1 pKa = 7.57HH2 pKa = 7.41IASNTGSARR11 pKa = 11.84GSGSLANSVRR21 pKa = 11.84NSGNLAANITILGNQGTASGGGVVNSVVNRR51 pKa = 11.84GNLSGAVAIVGNRR64 pKa = 11.84GTASNGGTVNSLVNTGTMAGKK85 pKa = 10.14VVIAGNQGSAGMGGTANSVINRR107 pKa = 11.84GVITGSVTIVGNNARR122 pKa = 11.84AGLGTTTASVRR133 pKa = 11.84NVGVLAGAAGVAGGAPWAASVGKK156 pKa = 8.72TITLPSTGVVNRR168 pKa = 11.84SVTVGPAVTVLNMM181 pKa = 3.79

Molecular weight: 17.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4770

0

4770

1558315

27

14037

326.7

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.244 ± 0.068

0.857 ± 0.014

5.342 ± 0.033

4.948 ± 0.043

3.329 ± 0.023

8.923 ± 0.055

2.193 ± 0.021

3.868 ± 0.027

2.624 ± 0.032

10.855 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.019

2.292 ± 0.036

5.598 ± 0.05

3.984 ± 0.025

7.398 ± 0.065

4.825 ± 0.048

5.107 ± 0.065

7.733 ± 0.035

1.481 ± 0.019

2.058 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski