Prochlorococcus phage P-TIM68

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0KWU4|A0A0K0KWU4_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-TIM68 OX=1542477 PE=4 SV=1
MM1 pKa = 7.87IDD3 pKa = 3.84LEE5 pKa = 4.5LTFDD9 pKa = 3.55EE10 pKa = 4.42QYY12 pKa = 11.6EE13 pKa = 4.52FIKK16 pKa = 10.82LYY18 pKa = 10.79DD19 pKa = 3.62ILRR22 pKa = 11.84DD23 pKa = 3.34MDD25 pKa = 4.95FEE27 pKa = 4.34LTPLQEE33 pKa = 4.54SVFEE37 pKa = 4.87KK38 pKa = 10.7IQFTPNYY45 pKa = 9.9SVYY48 pKa = 10.61QGAA51 pKa = 3.9

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0KVQ1|A0A0K0KVQ1_9CAUD Putative base plate hub assembly catalyst OS=Prochlorococcus phage P-TIM68 OX=1542477 PE=4 SV=1
MM1 pKa = 7.13KK2 pKa = 10.3QINLPMKK9 pKa = 10.33RR10 pKa = 11.84SFLEE14 pKa = 4.28FMSLCEE20 pKa = 3.89AVYY23 pKa = 10.86DD24 pKa = 3.84KK25 pKa = 11.53DD26 pKa = 3.9VMGRR30 pKa = 11.84SQIRR34 pKa = 11.84KK35 pKa = 7.13TGEE38 pKa = 3.62GGRR41 pKa = 11.84IQPKK45 pKa = 9.88RR46 pKa = 11.84KK47 pKa = 7.5QTEE50 pKa = 3.92PEE52 pKa = 3.75KK53 pKa = 10.88RR54 pKa = 11.84RR55 pKa = 11.84MKK57 pKa = 10.71AVGGGKK63 pKa = 8.23MAPAKK68 pKa = 9.59TYY70 pKa = 10.79KK71 pKa = 9.94DD72 pKa = 3.51RR73 pKa = 11.84KK74 pKa = 10.59DD75 pKa = 3.04IGQPNVKK82 pKa = 9.73RR83 pKa = 11.84SPAGRR88 pKa = 11.84QQQPTKK94 pKa = 10.63EE95 pKa = 3.95RR96 pKa = 11.84GTAGLSPRR104 pKa = 11.84EE105 pKa = 4.16QQRR108 pKa = 11.84KK109 pKa = 8.29AAMEE113 pKa = 3.55RR114 pKa = 11.84RR115 pKa = 11.84AAKK118 pKa = 10.26KK119 pKa = 9.91GVKK122 pKa = 8.89TKK124 pKa = 9.76TADD127 pKa = 3.13EE128 pKa = 4.33LLAKK132 pKa = 9.73KK133 pKa = 10.33AKK135 pKa = 10.66AKK137 pKa = 10.35VDD139 pKa = 3.9PNYY142 pKa = 10.55KK143 pKa = 8.44PQKK146 pKa = 9.11ASGMTRR152 pKa = 11.84AEE154 pKa = 3.95RR155 pKa = 11.84MSVVRR160 pKa = 11.84KK161 pKa = 10.62GEE163 pKa = 4.14TKK165 pKa = 10.23LRR167 pKa = 11.84NIMKK171 pKa = 10.1GQEE174 pKa = 3.37TDD176 pKa = 2.9KK177 pKa = 11.13YY178 pKa = 10.96KK179 pKa = 10.95KK180 pKa = 9.17EE181 pKa = 4.07TGQNPDD187 pKa = 3.4KK188 pKa = 10.4KK189 pKa = 10.89GKK191 pKa = 7.78MKK193 pKa = 10.58IMGRR197 pKa = 11.84VHH199 pKa = 6.15QRR201 pKa = 11.84MSTT204 pKa = 3.1

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

246

0

246

62701

36

4307

254.9

28.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.127 ± 0.165

0.935 ± 0.083

6.568 ± 0.132

6.094 ± 0.259

4.628 ± 0.138

7.847 ± 0.314

1.778 ± 0.115

6.802 ± 0.153

6.785 ± 0.45

7.352 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.164

5.946 ± 0.173

3.63 ± 0.12

3.443 ± 0.098

3.97 ± 0.112

7.159 ± 0.23

7.112 ± 0.349

6.623 ± 0.209

1.037 ± 0.102

4.038 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski