Paenibacillus sp. 7541

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4741 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A269WB89|A0A269WB89_9BACL AI-2E family transporter OS=Paenibacillus sp. 7541 OX=2026236 GN=CHH75_04050 PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.16SLKK5 pKa = 10.33KK6 pKa = 10.58SVVLLLSLSLLLAACGGDD24 pKa = 3.36KK25 pKa = 10.76TEE27 pKa = 4.99SDD29 pKa = 3.79PSSSNQEE36 pKa = 4.01DD37 pKa = 4.43VKK39 pKa = 10.68TEE41 pKa = 3.6AAAEE45 pKa = 3.96QEE47 pKa = 4.43PKK49 pKa = 10.25KK50 pKa = 10.48EE51 pKa = 4.26PKK53 pKa = 10.04NEE55 pKa = 3.83PAEE58 pKa = 4.24EE59 pKa = 4.15VSTTLFSNEE68 pKa = 4.15YY69 pKa = 9.77IQFEE73 pKa = 4.59YY74 pKa = 10.25PSTWVDD80 pKa = 3.45PQVDD84 pKa = 3.52NALILAAFVDD94 pKa = 4.45ANAAGGFADD103 pKa = 3.82NVNIVLEE110 pKa = 4.47DD111 pKa = 3.4SAMTAQEE118 pKa = 4.2AANQAVSDD126 pKa = 4.11LTSSAGGEE134 pKa = 4.25VIQNYY139 pKa = 7.15QQISYY144 pKa = 9.95TDD146 pKa = 3.96ALNGDD151 pKa = 4.11YY152 pKa = 10.9DD153 pKa = 4.13AGILTGSYY161 pKa = 8.64TQGQSGVGVMLTQYY175 pKa = 10.33FVATGDD181 pKa = 3.34QMYY184 pKa = 10.24TMSLSFAKK192 pKa = 9.31DD193 pKa = 3.04TYY195 pKa = 11.31EE196 pKa = 5.73DD197 pKa = 3.4GGEE200 pKa = 3.91QLKK203 pKa = 10.6IPVGGGYY210 pKa = 10.42LCGSLFISTLL220 pKa = 3.44

Molecular weight: 23.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A269W035|A0A269W035_9BACL Alcohol dehydrogenase OS=Paenibacillus sp. 7541 OX=2026236 GN=CHH75_13955 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.55KK14 pKa = 8.83VHH16 pKa = 5.81GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTKK25 pKa = 10.15NGRR28 pKa = 11.84KK29 pKa = 8.44VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4741

0

4741

1530362

26

2123

322.8

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.414 ± 0.038

0.728 ± 0.011

5.164 ± 0.027

7.011 ± 0.042

3.954 ± 0.029

7.598 ± 0.032

2.215 ± 0.018

6.528 ± 0.03

4.758 ± 0.029

10.101 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.986 ± 0.018

3.518 ± 0.023

4.239 ± 0.021

3.929 ± 0.021

5.436 ± 0.032

6.228 ± 0.028

5.209 ± 0.025

7.136 ± 0.031

1.312 ± 0.014

3.537 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski