Amino acid dipepetide frequency for Escherichia phage vB_EcoP_F

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.911AlaAla: 9.911 ± 1.261
0.926AlaCys: 0.926 ± 0.347
5.558AlaAsp: 5.558 ± 0.844
5.743AlaGlu: 5.743 ± 0.802
3.057AlaPhe: 3.057 ± 0.443
6.762AlaGly: 6.762 ± 0.873
0.834AlaHis: 0.834 ± 0.273
5.002AlaIle: 5.002 ± 0.792
5.835AlaLys: 5.835 ± 0.662
6.577AlaLeu: 6.577 ± 0.999
2.594AlaMet: 2.594 ± 0.546
3.612AlaAsn: 3.612 ± 0.536
3.149AlaPro: 3.149 ± 0.61
3.057AlaGln: 3.057 ± 0.563
3.612AlaArg: 3.612 ± 0.519
5.187AlaSer: 5.187 ± 0.546
3.335AlaThr: 3.335 ± 0.491
6.391AlaVal: 6.391 ± 0.953
1.76AlaTrp: 1.76 ± 0.478
2.686AlaTyr: 2.686 ± 0.478
0.0AlaXaa: 0.0 ± 0.0
Cys
0.556CysAla: 0.556 ± 0.171
0.0CysCys: 0.0 ± 0.0
0.648CysAsp: 0.648 ± 0.378
0.926CysGlu: 0.926 ± 0.262
0.648CysPhe: 0.648 ± 0.243
0.834CysGly: 0.834 ± 0.302
0.185CysHis: 0.185 ± 0.137
0.278CysIle: 0.278 ± 0.142
0.556CysLys: 0.556 ± 0.244
0.834CysLeu: 0.834 ± 0.34
0.648CysMet: 0.648 ± 0.279
0.371CysAsn: 0.371 ± 0.213
0.371CysPro: 0.371 ± 0.208
0.185CysGln: 0.185 ± 0.15
0.648CysArg: 0.648 ± 0.284
0.556CysSer: 0.556 ± 0.249
0.093CysThr: 0.093 ± 0.103
0.463CysVal: 0.463 ± 0.202
0.185CysTrp: 0.185 ± 0.117
0.278CysTyr: 0.278 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
6.669AspAla: 6.669 ± 0.732
0.463AspCys: 0.463 ± 0.316
4.631AspAsp: 4.631 ± 0.796
4.076AspGlu: 4.076 ± 0.609
2.686AspPhe: 2.686 ± 0.484
6.577AspGly: 6.577 ± 0.763
1.297AspHis: 1.297 ± 0.294
3.149AspIle: 3.149 ± 0.498
3.52AspLys: 3.52 ± 0.631
5.372AspLeu: 5.372 ± 0.725
1.853AspMet: 1.853 ± 0.397
2.964AspAsn: 2.964 ± 0.49
2.223AspPro: 2.223 ± 0.538
1.945AspGln: 1.945 ± 0.423
2.594AspArg: 2.594 ± 0.491
2.964AspSer: 2.964 ± 0.447
4.724AspThr: 4.724 ± 0.621
5.094AspVal: 5.094 ± 0.665
1.112AspTrp: 1.112 ± 0.368
2.408AspTyr: 2.408 ± 0.462
0.0AspXaa: 0.0 ± 0.0
Glu
6.577GluAla: 6.577 ± 1.057
0.556GluCys: 0.556 ± 0.205
5.28GluAsp: 5.28 ± 0.733
5.372GluGlu: 5.372 ± 0.783
2.964GluPhe: 2.964 ± 0.45
5.094GluGly: 5.094 ± 0.965
1.204GluHis: 1.204 ± 0.334
2.316GluIle: 2.316 ± 0.472
3.427GluLys: 3.427 ± 0.548
5.372GluLeu: 5.372 ± 0.828
2.223GluMet: 2.223 ± 0.498
1.853GluAsn: 1.853 ± 0.356
2.594GluPro: 2.594 ± 0.474
2.871GluGln: 2.871 ± 0.553
4.261GluArg: 4.261 ± 0.566
4.168GluSer: 4.168 ± 0.614
3.612GluThr: 3.612 ± 0.485
4.261GluVal: 4.261 ± 0.748
1.575GluTrp: 1.575 ± 0.371
3.057GluTyr: 3.057 ± 0.437
0.0GluXaa: 0.0 ± 0.0
Phe
3.242PheAla: 3.242 ± 0.503
0.371PheCys: 0.371 ± 0.205
2.501PheAsp: 2.501 ± 0.45
1.667PheGlu: 1.667 ± 0.38
1.297PhePhe: 1.297 ± 0.368
2.316PheGly: 2.316 ± 0.439
0.834PheHis: 0.834 ± 0.24
2.223PheIle: 2.223 ± 0.447
3.242PheLys: 3.242 ± 0.66
3.149PheLeu: 3.149 ± 0.441
0.741PheMet: 0.741 ± 0.281
2.223PheAsn: 2.223 ± 0.4
1.575PhePro: 1.575 ± 0.408
0.834PheGln: 0.834 ± 0.27
1.389PheArg: 1.389 ± 0.302
2.501PheSer: 2.501 ± 0.397
2.038PheThr: 2.038 ± 0.369
3.057PheVal: 3.057 ± 0.599
0.371PheTrp: 0.371 ± 0.179
1.112PheTyr: 1.112 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
6.113GlyAla: 6.113 ± 1.013
0.371GlyCys: 0.371 ± 0.189
6.113GlyAsp: 6.113 ± 0.912
5.28GlyGlu: 5.28 ± 0.785
2.13GlyPhe: 2.13 ± 0.404
5.094GlyGly: 5.094 ± 0.562
1.204GlyHis: 1.204 ± 0.302
4.168GlyIle: 4.168 ± 0.514
5.28GlyLys: 5.28 ± 0.914
6.021GlyLeu: 6.021 ± 0.911
2.501GlyMet: 2.501 ± 0.57
2.686GlyAsn: 2.686 ± 0.501
1.112GlyPro: 1.112 ± 0.247
3.149GlyGln: 3.149 ± 0.405
4.909GlyArg: 4.909 ± 0.755
6.299GlySer: 6.299 ± 0.899
4.168GlyThr: 4.168 ± 0.465
5.094GlyVal: 5.094 ± 0.562
1.204GlyTrp: 1.204 ± 0.299
3.798GlyTyr: 3.798 ± 0.511
0.0GlyXaa: 0.0 ± 0.0
His
0.556HisAla: 0.556 ± 0.182
0.278HisCys: 0.278 ± 0.132
1.389HisAsp: 1.389 ± 0.495
1.76HisGlu: 1.76 ± 0.506
0.648HisPhe: 0.648 ± 0.264
1.019HisGly: 1.019 ± 0.33
0.463HisHis: 0.463 ± 0.194
1.112HisIle: 1.112 ± 0.235
1.019HisLys: 1.019 ± 0.272
2.13HisLeu: 2.13 ± 0.503
0.648HisMet: 0.648 ± 0.207
0.648HisAsn: 0.648 ± 0.243
0.648HisPro: 0.648 ± 0.2
0.463HisGln: 0.463 ± 0.175
1.204HisArg: 1.204 ± 0.35
0.926HisSer: 0.926 ± 0.263
1.297HisThr: 1.297 ± 0.295
1.389HisVal: 1.389 ± 0.374
0.371HisTrp: 0.371 ± 0.205
0.741HisTyr: 0.741 ± 0.297
0.0HisXaa: 0.0 ± 0.0
Ile
3.798IleAla: 3.798 ± 0.708
0.741IleCys: 0.741 ± 0.321
3.427IleAsp: 3.427 ± 0.492
3.427IleGlu: 3.427 ± 0.431
1.019IlePhe: 1.019 ± 0.341
4.076IleGly: 4.076 ± 0.522
1.482IleHis: 1.482 ± 0.411
2.13IleIle: 2.13 ± 0.459
3.149IleLys: 3.149 ± 0.563
3.335IleLeu: 3.335 ± 0.585
1.019IleMet: 1.019 ± 0.263
2.501IleAsn: 2.501 ± 0.474
2.223IlePro: 2.223 ± 0.541
1.853IleGln: 1.853 ± 0.482
2.779IleArg: 2.779 ± 0.494
3.242IleSer: 3.242 ± 0.633
3.705IleThr: 3.705 ± 0.554
4.353IleVal: 4.353 ± 0.412
0.648IleTrp: 0.648 ± 0.217
1.575IleTyr: 1.575 ± 0.317
0.0IleXaa: 0.0 ± 0.0
Lys
6.947LysAla: 6.947 ± 0.908
0.556LysCys: 0.556 ± 0.251
4.168LysAsp: 4.168 ± 0.526
3.798LysGlu: 3.798 ± 0.644
2.13LysPhe: 2.13 ± 0.572
4.076LysGly: 4.076 ± 0.571
1.482LysHis: 1.482 ± 0.462
2.316LysIle: 2.316 ± 0.353
3.89LysLys: 3.89 ± 0.867
4.724LysLeu: 4.724 ± 0.7
2.13LysMet: 2.13 ± 0.343
1.667LysAsn: 1.667 ± 0.336
2.871LysPro: 2.871 ± 0.632
1.76LysGln: 1.76 ± 0.487
3.89LysArg: 3.89 ± 0.712
4.446LysSer: 4.446 ± 0.61
3.612LysThr: 3.612 ± 0.527
5.743LysVal: 5.743 ± 0.865
1.389LysTrp: 1.389 ± 0.409
2.316LysTyr: 2.316 ± 0.464
0.0LysXaa: 0.0 ± 0.0
Leu
5.928LeuAla: 5.928 ± 0.972
0.371LeuCys: 0.371 ± 0.174
4.446LeuAsp: 4.446 ± 0.457
5.928LeuGlu: 5.928 ± 0.831
2.501LeuPhe: 2.501 ± 0.457
4.724LeuGly: 4.724 ± 0.582
1.389LeuHis: 1.389 ± 0.493
4.076LeuIle: 4.076 ± 0.615
6.391LeuLys: 6.391 ± 0.939
4.631LeuLeu: 4.631 ± 0.651
3.242LeuMet: 3.242 ± 0.54
4.446LeuAsn: 4.446 ± 0.65
3.057LeuPro: 3.057 ± 0.376
3.705LeuGln: 3.705 ± 0.67
4.076LeuArg: 4.076 ± 0.453
5.372LeuSer: 5.372 ± 0.95
4.539LeuThr: 4.539 ± 0.623
4.539LeuVal: 4.539 ± 0.561
0.926LeuTrp: 0.926 ± 0.325
2.871LeuTyr: 2.871 ± 0.598
0.0LeuXaa: 0.0 ± 0.0
Met
3.242MetAla: 3.242 ± 0.578
0.463MetCys: 0.463 ± 0.198
1.297MetAsp: 1.297 ± 0.303
2.13MetGlu: 2.13 ± 0.375
1.204MetPhe: 1.204 ± 0.364
2.686MetGly: 2.686 ± 0.522
0.278MetHis: 0.278 ± 0.141
1.297MetIle: 1.297 ± 0.239
1.112MetLys: 1.112 ± 0.335
2.316MetLeu: 2.316 ± 0.443
0.463MetMet: 0.463 ± 0.2
0.834MetAsn: 0.834 ± 0.255
1.019MetPro: 1.019 ± 0.32
0.741MetGln: 0.741 ± 0.286
1.297MetArg: 1.297 ± 0.373
2.038MetSer: 2.038 ± 0.478
1.853MetThr: 1.853 ± 0.399
2.779MetVal: 2.779 ± 0.468
0.278MetTrp: 0.278 ± 0.172
1.019MetTyr: 1.019 ± 0.237
0.0MetXaa: 0.0 ± 0.0
Asn
4.631AsnAla: 4.631 ± 0.697
0.648AsnCys: 0.648 ± 0.227
2.13AsnAsp: 2.13 ± 0.493
2.686AsnGlu: 2.686 ± 0.563
1.575AsnPhe: 1.575 ± 0.33
4.261AsnGly: 4.261 ± 0.693
0.556AsnHis: 0.556 ± 0.191
2.501AsnIle: 2.501 ± 0.4
2.501AsnLys: 2.501 ± 0.568
2.316AsnLeu: 2.316 ± 0.512
1.019AsnMet: 1.019 ± 0.299
1.76AsnAsn: 1.76 ± 0.41
2.871AsnPro: 2.871 ± 0.53
1.389AsnGln: 1.389 ± 0.276
2.316AsnArg: 2.316 ± 0.563
2.501AsnSer: 2.501 ± 0.55
2.038AsnThr: 2.038 ± 0.381
2.964AsnVal: 2.964 ± 0.586
0.278AsnTrp: 0.278 ± 0.181
1.945AsnTyr: 1.945 ± 0.481
0.0AsnXaa: 0.0 ± 0.0
Pro
2.594ProAla: 2.594 ± 0.6
0.463ProCys: 0.463 ± 0.234
2.779ProAsp: 2.779 ± 0.544
2.779ProGlu: 2.779 ± 0.501
1.389ProPhe: 1.389 ± 0.328
1.482ProGly: 1.482 ± 0.267
0.556ProHis: 0.556 ± 0.187
1.945ProIle: 1.945 ± 0.408
3.057ProLys: 3.057 ± 0.589
2.223ProLeu: 2.223 ± 0.429
1.112ProMet: 1.112 ± 0.308
2.13ProAsn: 2.13 ± 0.414
0.463ProPro: 0.463 ± 0.224
1.482ProGln: 1.482 ± 0.451
1.575ProArg: 1.575 ± 0.376
3.149ProSer: 3.149 ± 0.467
2.964ProThr: 2.964 ± 0.396
3.242ProVal: 3.242 ± 0.312
0.926ProTrp: 0.926 ± 0.252
1.389ProTyr: 1.389 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
3.89GlnAla: 3.89 ± 0.593
0.278GlnCys: 0.278 ± 0.171
3.149GlnAsp: 3.149 ± 0.78
2.038GlnGlu: 2.038 ± 0.435
1.76GlnPhe: 1.76 ± 0.385
2.686GlnGly: 2.686 ± 0.468
0.556GlnHis: 0.556 ± 0.234
1.389GlnIle: 1.389 ± 0.4
2.13GlnLys: 2.13 ± 0.448
4.076GlnLeu: 4.076 ± 0.585
1.204GlnMet: 1.204 ± 0.423
1.204GlnAsn: 1.204 ± 0.464
1.204GlnPro: 1.204 ± 0.344
2.13GlnGln: 2.13 ± 0.549
2.594GlnArg: 2.594 ± 0.694
2.686GlnSer: 2.686 ± 0.484
1.945GlnThr: 1.945 ± 0.551
2.408GlnVal: 2.408 ± 0.478
0.556GlnTrp: 0.556 ± 0.245
1.019GlnTyr: 1.019 ± 0.359
0.0GlnXaa: 0.0 ± 0.0
Arg
3.798ArgAla: 3.798 ± 0.594
0.371ArgCys: 0.371 ± 0.193
4.261ArgAsp: 4.261 ± 0.583
3.335ArgGlu: 3.335 ± 0.62
2.686ArgPhe: 2.686 ± 0.394
3.612ArgGly: 3.612 ± 0.525
0.741ArgHis: 0.741 ± 0.233
4.168ArgIle: 4.168 ± 0.719
3.612ArgLys: 3.612 ± 0.613
5.558ArgLeu: 5.558 ± 0.788
1.112ArgMet: 1.112 ± 0.345
2.223ArgAsn: 2.223 ± 0.418
1.667ArgPro: 1.667 ± 0.366
2.686ArgGln: 2.686 ± 0.478
2.594ArgArg: 2.594 ± 0.5
3.242ArgSer: 3.242 ± 0.552
1.853ArgThr: 1.853 ± 0.4
2.594ArgVal: 2.594 ± 0.67
0.741ArgTrp: 0.741 ± 0.227
1.853ArgTyr: 1.853 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
4.446SerAla: 4.446 ± 0.605
0.926SerCys: 0.926 ± 0.359
4.909SerAsp: 4.909 ± 0.541
4.168SerGlu: 4.168 ± 0.711
2.686SerPhe: 2.686 ± 0.496
6.021SerGly: 6.021 ± 0.914
2.408SerHis: 2.408 ± 0.46
2.964SerIle: 2.964 ± 0.614
3.149SerLys: 3.149 ± 0.566
3.983SerLeu: 3.983 ± 0.573
1.389SerMet: 1.389 ± 0.311
3.52SerAsn: 3.52 ± 0.728
2.686SerPro: 2.686 ± 0.393
2.686SerGln: 2.686 ± 0.522
3.612SerArg: 3.612 ± 0.697
4.353SerSer: 4.353 ± 0.631
3.057SerThr: 3.057 ± 0.492
3.798SerVal: 3.798 ± 0.538
0.834SerTrp: 0.834 ± 0.226
2.686SerTyr: 2.686 ± 0.637
0.0SerXaa: 0.0 ± 0.0
Thr
3.242ThrAla: 3.242 ± 0.722
0.278ThrCys: 0.278 ± 0.191
3.242ThrAsp: 3.242 ± 0.68
4.724ThrGlu: 4.724 ± 0.578
2.038ThrPhe: 2.038 ± 0.425
5.187ThrGly: 5.187 ± 0.433
0.648ThrHis: 0.648 ± 0.236
4.168ThrIle: 4.168 ± 0.666
2.871ThrLys: 2.871 ± 0.537
4.539ThrLeu: 4.539 ± 0.681
1.482ThrMet: 1.482 ± 0.336
2.13ThrAsn: 2.13 ± 0.446
3.057ThrPro: 3.057 ± 0.408
2.408ThrGln: 2.408 ± 0.441
2.223ThrArg: 2.223 ± 0.442
2.779ThrSer: 2.779 ± 0.617
3.798ThrThr: 3.798 ± 0.602
5.002ThrVal: 5.002 ± 0.597
0.741ThrTrp: 0.741 ± 0.259
1.482ThrTyr: 1.482 ± 0.293
0.0ThrXaa: 0.0 ± 0.0
Val
4.909ValAla: 4.909 ± 0.668
0.556ValCys: 0.556 ± 0.194
2.871ValAsp: 2.871 ± 0.62
5.372ValGlu: 5.372 ± 0.807
2.594ValPhe: 2.594 ± 0.515
6.113ValGly: 6.113 ± 0.646
1.389ValHis: 1.389 ± 0.517
2.964ValIle: 2.964 ± 0.434
5.835ValLys: 5.835 ± 0.771
5.372ValLeu: 5.372 ± 0.626
1.853ValMet: 1.853 ± 0.511
3.52ValAsn: 3.52 ± 0.589
3.057ValPro: 3.057 ± 0.451
3.057ValGln: 3.057 ± 0.517
4.261ValArg: 4.261 ± 0.613
4.817ValSer: 4.817 ± 0.623
4.631ValThr: 4.631 ± 0.64
6.484ValVal: 6.484 ± 0.896
0.741ValTrp: 0.741 ± 0.286
2.871ValTyr: 2.871 ± 0.535
0.0ValXaa: 0.0 ± 0.0
Trp
0.371TrpAla: 0.371 ± 0.152
0.185TrpCys: 0.185 ± 0.126
0.926TrpAsp: 0.926 ± 0.307
1.112TrpGlu: 1.112 ± 0.319
0.463TrpPhe: 0.463 ± 0.197
1.112TrpGly: 1.112 ± 0.342
0.556TrpHis: 0.556 ± 0.222
0.463TrpIle: 0.463 ± 0.212
1.297TrpLys: 1.297 ± 0.398
2.223TrpLeu: 2.223 ± 0.407
0.093TrpMet: 0.093 ± 0.093
0.834TrpAsn: 0.834 ± 0.276
0.371TrpPro: 0.371 ± 0.175
0.648TrpGln: 0.648 ± 0.253
0.556TrpArg: 0.556 ± 0.205
1.482TrpSer: 1.482 ± 0.558
0.648TrpThr: 0.648 ± 0.255
1.389TrpVal: 1.389 ± 0.38
0.185TrpTrp: 0.185 ± 0.128
0.371TrpTyr: 0.371 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.168TyrAla: 4.168 ± 0.71
0.463TyrCys: 0.463 ± 0.197
2.594TyrAsp: 2.594 ± 0.594
2.316TyrGlu: 2.316 ± 0.517
1.204TyrPhe: 1.204 ± 0.271
2.964TyrGly: 2.964 ± 0.587
0.648TyrHis: 0.648 ± 0.218
1.853TyrIle: 1.853 ± 0.529
2.038TyrLys: 2.038 ± 0.422
2.408TyrLeu: 2.408 ± 0.406
0.926TyrMet: 0.926 ± 0.302
1.76TyrAsn: 1.76 ± 0.412
1.482TyrPro: 1.482 ± 0.399
1.853TyrGln: 1.853 ± 0.671
2.316TyrArg: 2.316 ± 0.405
1.667TyrSer: 1.667 ± 0.426
2.13TyrThr: 2.13 ± 0.451
2.223TyrVal: 2.223 ± 0.526
0.463TyrTrp: 0.463 ± 0.197
1.667TyrTyr: 1.667 ± 0.461
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (10797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski